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The map label for this gene is oprM [H]

Identifier: 73540920

GI number: 73540920

Start: 1309084

End: 1310547

Strand: Direct

Name: oprM [H]

Synonym: Reut_A1225

Alternate gene names: 73540920

Gene position: 1309084-1310547 (Clockwise)

Preceding gene: 73540919

Following gene: 73540921

Centisome position: 34.39

GC content: 67.96

Gene sequence:

>1464_bases
GTGAGCTGGCATTGGCCCTTGCGGACGCTCCCGATGGCCGTATTTGCGGTGGTGGCGCTTTGCGGCTGCGCGGTGGGACC
CGACTTTGTCCCGCCTGAGGAACCTGCCATTTCACAGTATGTTCGCGGCGGGGACGCCAGCAGCACGCCCGCCGGCGACG
GACGGGCCCAGCACTTTGCTGCCGGCGCGATGGTGCCCGCCGACTGGTGGCGCATGTTTGGCAGTGCGGAACTGGACCAG
GCCGTAGATGGAGCGTTGGCCGCGAATCCGAGTCTGCAGGTCGCTGAAGCGAACCTGCGGCAGGCTGATGCGGTAGCGCG
TTCCGGCGCCGGCGTGTTCTATCCGCAGGTTGCGGCACAGGCGCAAGCGTCGCGACAGACGACGACGCCCGTCCGGTTGG
GAAGCAGCGCCCCGCCCGGCATATTCAATGTCTTCACGCTGGGCGCCACGATCAGCTATGCGCTGGACCTGTTCGGCGGC
AACCGCCGGGCGGTGGAAGCGCTGGCTGCGCAAGCAGAAGTGCAGCGCTACGCCGTTGGTGCGGCCTGGCTCACGCTGAC
CGGCACACTGGTCAATACCGCGATCGCGCGCGCCGGCTATGCCGCCCAGATCGCTGCCACGGATACGCTGGTGAAGATCG
TGCGCGAGCAAGTCGACGTCACGCGCGCGCAGGTAAGCGCCGGCATGACGACCCACGCCAGCGAACTGAGCCTGCTGAGC
CAGCTTGGGTCGCTGGAAGCGACACTTCCTCCGCTGGCGCAACGCATGGATCAGGCGGATCACCTTGGCGCGACGCTTGC
CGGGCGCTTCCCTTCAGAGGCCGGGCCGCTGGTCCTTTCGCTCGATTCGCTGGTGTTGCCGTCCGACCTGCCGCGCATGC
TGCCTTCCGAGCTGGTCCGGCACAGGCCAGACGTGCTGGCCGCGCAGGCAGAACTGCACGTGGCCGCGGCACAAGTCGGC
GTGGCAACGGCTGCCATGCTCCCGAACATTGTGCTGAGTGCCAGCTATGGCCGCGAAGGCGTACAGCCTGGCAGCTTGCT
GGGTGCGGGCGGAGGCGTCTGGGGCATCGCTGCGGCCGCCTTTGCGCCGGTGTTCCAGGGCGGCACGCTGTACTTCAGGC
GCCGCGCGGCACAAGCGCAATTTGAAGCGGCACAGGCCAACTACCGGCAAGTCGTGCTGGCGGCCTTTGCCCAGGTGGCC
GATGCACTGCGTGCGCTGGAACACGACGCACTCACGGTGGATGCGCAGGACCGCGCGCGGCAGGCCGCGGGGGAGCTGAT
GACCGAGGTGCAGGCCAACTACCGGGCCGGCGCGGCGGGATACCTGCAGCTTCTCGTCGCCAATGCGCAGTACCAGCAGG
CGACGATCGGCTATATACAGGCTCACACCCAAAGGCTGCAGGACACCGCAGCATTGTTCCTTGCCTTGGGCGCCGGCTGG
GAAGCCGCGTCGCCGGCTCGTTGA

Upstream 100 bases:

>100_bases
TCGACGTACGCGTACTGCCGGTAATCTTCCGCTTCAGGCCGCCCGCCCAGCCACCGATCTACCCGGGGCAACTGGTCGAT
GTCTATCTGGGCGGGACACC

Downstream 100 bases:

>100_bases
CGCATATCAACGGATATCAGGCGCTACCGCATACGCTGAGTCAGGGGAAGGCCAAGGCGGGCAACGCTGGGTGAGATGCC
GGGGCAGTCCGCTGATCATC

Product: RND efflux system, outer membrane lipoprotein, NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MSWHWPLRTLPMAVFAVVALCGCAVGPDFVPPEEPAISQYVRGGDASSTPAGDGRAQHFAAGAMVPADWWRMFGSAELDQ
AVDGALAANPSLQVAEANLRQADAVARSGAGVFYPQVAAQAQASRQTTTPVRLGSSAPPGIFNVFTLGATISYALDLFGG
NRRAVEALAAQAEVQRYAVGAAWLTLTGTLVNTAIARAGYAAQIAATDTLVKIVREQVDVTRAQVSAGMTTHASELSLLS
QLGSLEATLPPLAQRMDQADHLGATLAGRFPSEAGPLVLSLDSLVLPSDLPRMLPSELVRHRPDVLAAQAELHVAAAQVG
VATAAMLPNIVLSASYGREGVQPGSLLGAGGGVWGIAAAAFAPVFQGGTLYFRRRAAQAQFEAAQANYRQVVLAAFAQVA
DALRALEHDALTVDAQDRARQAAGELMTEVQANYRAGAAGYLQLLVANAQYQQATIGYIQAHTQRLQDTAALFLALGAGW
EAASPAR

Sequences:

>Translated_487_residues
MSWHWPLRTLPMAVFAVVALCGCAVGPDFVPPEEPAISQYVRGGDASSTPAGDGRAQHFAAGAMVPADWWRMFGSAELDQ
AVDGALAANPSLQVAEANLRQADAVARSGAGVFYPQVAAQAQASRQTTTPVRLGSSAPPGIFNVFTLGATISYALDLFGG
NRRAVEALAAQAEVQRYAVGAAWLTLTGTLVNTAIARAGYAAQIAATDTLVKIVREQVDVTRAQVSAGMTTHASELSLLS
QLGSLEATLPPLAQRMDQADHLGATLAGRFPSEAGPLVLSLDSLVLPSDLPRMLPSELVRHRPDVLAAQAELHVAAAQVG
VATAAMLPNIVLSASYGREGVQPGSLLGAGGGVWGIAAAAFAPVFQGGTLYFRRRAAQAQFEAAQANYRQVVLAAFAQVA
DALRALEHDALTVDAQDRARQAAGELMTEVQANYRAGAAGYLQLLVANAQYQQATIGYIQAHTQRLQDTAALFLALGAGW
EAASPAR
>Mature_486_residues
SWHWPLRTLPMAVFAVVALCGCAVGPDFVPPEEPAISQYVRGGDASSTPAGDGRAQHFAAGAMVPADWWRMFGSAELDQA
VDGALAANPSLQVAEANLRQADAVARSGAGVFYPQVAAQAQASRQTTTPVRLGSSAPPGIFNVFTLGATISYALDLFGGN
RRAVEALAAQAEVQRYAVGAAWLTLTGTLVNTAIARAGYAAQIAATDTLVKIVREQVDVTRAQVSAGMTTHASELSLLSQ
LGSLEATLPPLAQRMDQADHLGATLAGRFPSEAGPLVLSLDSLVLPSDLPRMLPSELVRHRPDVLAAQAELHVAAAQVGV
ATAAMLPNIVLSASYGREGVQPGSLLGAGGGVWGIAAAAFAPVFQGGTLYFRRRAAQAQFEAAQANYRQVVLAAFAQVAD
ALRALEHDALTVDAQDRARQAAGELMTEVQANYRAGAAGYLQLLVANAQYQQATIGYIQAHTQRLQDTAALFLALGAGWE
AASPAR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=481, Percent_Identity=25.5717255717256, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 50754; Mature: 50623

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCC
>Mature Secondary Structure 
SWHWPLRTLPMAVFAVVALCGCAVGPDFVPPEEPAISQYVRGGDASSTPAGDGRAQHFA
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHH
AGAMVPADWWRMFGSAELDQAVDGALAANPSLQVAEANLRQADAVARSGAGVFYPQVAAQ
CCCCCCHHHHHHHCCCHHHHHHCCHHCCCCCCEEHHHHHHHHHHHHHCCCCCCCHHHHHH
AQASRQTTTPVRLGSSAPPGIFNVFTLGATISYALDLFGGNRRAVEALAAQAEVQRYAVG
HHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AAWLTLTGTLVNTAIARAGYAAQIAATDTLVKIVREQVDVTRAQVSAGMTTHASELSLLS
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
QLGSLEATLPPLAQRMDQADHLGATLAGRFPSEAGPLVLSLDSLVLPSDLPRMLPSELVR
HHCCCCCCCCHHHHHHHHHHHCCCHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHH
HRPDVLAAQAELHVAAAQVGVATAAMLPNIVLSASYGREGVQPGSLLGAGGGVWGIAAAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCEEECCCCHHHHHHHH
FAPVFQGGTLYFRRRAAQAQFEAAQANYRQVVLAAFAQVADALRALEHDALTVDAQDRAR
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHH
QAAGELMTEVQANYRAGAAGYLQLLVANAQYQQATIGYIQAHTQRLQDTAALFLALGAGW
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EAASPAR
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]