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The map label for this gene is trmJ [H]

Identifier: 73540818

GI number: 73540818

Start: 1209025

End: 1209969

Strand: Direct

Name: trmJ [H]

Synonym: Reut_A1116

Alternate gene names: 73540818

Gene position: 1209025-1209969 (Clockwise)

Preceding gene: 73540811

Following gene: 73540819

Centisome position: 31.76

GC content: 67.83

Gene sequence:

>945_bases
GTGCTGCAAGCGTCTCGCTGGCAAGCAAAATATCGGCGCCGCGCCGAAGAAGAAGCTGAATTGTATATGAACCCGGCAAA
CGAGACGAGCCAGGCCGCCGGAACGGCCGCGCTGGCCATGCCTGCGGACGGCATGTTCGCCCGCGTGCGCTTTGTGCTGG
TCGAAACCAGCCACCCCGGCAATGTCGGCTCGGTGGCGCGCGCGATCAAGACCATGGGCTTTGGCAGCCTGGTGCTGGTC
TCGCCACGTGAGCCTGGCGTGCGCGAGCACCCGGATGCCGTGGCCATGGCCAGCGGCGCTGACGACGTGCTGGCCGGCGC
GGTGATTGTGGACAGCATCGAAGCCGCGCTGGAGGGGGCCACGCAGACCGTGGCGATGACCGCACGGCAACGCGAGTTCG
GCCCGCCCCGGCTGCTGCCGCGTGACGCTGCCGCACGCGGTGCCGAGTCGCTGGCTGCCCGGGCCGGGGACGAGATCGCA
TTCGTATTCGGCAACGAGCGCTACGGCCTGCCCAACGACGTCGTCGAGCGCTGCGTGGCGGTGACGCATATCCCCGCCAA
TCCGGTCTATGCCTCGCTCAATCTGGCCCAGGCCGTGCAGCTCATTGCCTACGAGATGCGCCTGGCGCTGCTGGACCGGG
CGCAGGCCGCGCCGGACGACAGTGGCAATATCGGCTATGCTGGTGAGCCGGCGACGGCGGAGCAGGTCGAATCGATGTTC
GGCCACCTGCAGCAGGGGCTGGAAGCGATTGGCTTCCTGGACCCGGCCAATCCGCGCAAGCTGATGACGCGCCTGCGGCG
GCTGCTCGCACGTGCCGGGTTGGAGCGTGAGGAAGTGAACATCCTGCGCGGCATCGCCAAGCACATGCTGATCGCCGTGC
GCGGCGGTCGCACCAAGCCAGAACAATCAGGGCCGTCCACACCGGCCAGGCAAGAGGAACATTGA

Upstream 100 bases:

>100_bases
CCGCACCGGATTTCCGCCATGGCGCGAACGATTTCGCGCACCGGGGTCCGGGCAGCTGACAACACAACGAATTGTAGAAG
AACGTGGGGCGTCGCCATAT

Downstream 100 bases:

>100_bases
ACCGTGGCGCAGAACCAGGCCCAGACACCGAGTTTCCCCACTGCGACGCGCCGATGCGCGCGCAGGCCGGTGGCTGGTCG
CGCGTCCCTGTCCCAGCGAT

Product: RNA methyltransferase TrmH, group 1

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MLQASRWQAKYRRRAEEEAELYMNPANETSQAAGTAALAMPADGMFARVRFVLVETSHPGNVGSVARAIKTMGFGSLVLV
SPREPGVREHPDAVAMASGADDVLAGAVIVDSIEAALEGATQTVAMTARQREFGPPRLLPRDAAARGAESLAARAGDEIA
FVFGNERYGLPNDVVERCVAVTHIPANPVYASLNLAQAVQLIAYEMRLALLDRAQAAPDDSGNIGYAGEPATAEQVESMF
GHLQQGLEAIGFLDPANPRKLMTRLRRLLARAGLEREEVNILRGIAKHMLIAVRGGRTKPEQSGPSTPARQEEH

Sequences:

>Translated_314_residues
MLQASRWQAKYRRRAEEEAELYMNPANETSQAAGTAALAMPADGMFARVRFVLVETSHPGNVGSVARAIKTMGFGSLVLV
SPREPGVREHPDAVAMASGADDVLAGAVIVDSIEAALEGATQTVAMTARQREFGPPRLLPRDAAARGAESLAARAGDEIA
FVFGNERYGLPNDVVERCVAVTHIPANPVYASLNLAQAVQLIAYEMRLALLDRAQAAPDDSGNIGYAGEPATAEQVESMF
GHLQQGLEAIGFLDPANPRKLMTRLRRLLARAGLEREEVNILRGIAKHMLIAVRGGRTKPEQSGPSTPARQEEH
>Mature_314_residues
MLQASRWQAKYRRRAEEEAELYMNPANETSQAAGTAALAMPADGMFARVRFVLVETSHPGNVGSVARAIKTMGFGSLVLV
SPREPGVREHPDAVAMASGADDVLAGAVIVDSIEAALEGATQTVAMTARQREFGPPRLLPRDAAARGAESLAARAGDEIA
FVFGNERYGLPNDVVERCVAVTHIPANPVYASLNLAQAVQLIAYEMRLALLDRAQAAPDDSGNIGYAGEPATAEQVESMF
GHLQQGLEAIGFLDPANPRKLMTRLRRLLARAGLEREEVNILRGIAKHMLIAVRGGRTKPEQSGPSTPARQEEH

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=242, Percent_Identity=45.0413223140496, Blast_Score=190, Evalue=8e-50,
Organism=Escherichia coli, GI1790865, Length=167, Percent_Identity=31.7365269461078, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 33667; Mature: 33667

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQASRWQAKYRRRAEEEAELYMNPANETSQAAGTAALAMPADGMFARVRFVLVETSHPG
CCCCHHHHHHHHHCCHHHHHEEECCCCCHHHHCCCEEEECCCCCCEEEEEEEEEECCCCC
NVGSVARAIKTMGFGSLVLVSPREPGVREHPDAVAMASGADDVLAGAVIVDSIEAALEGA
CHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCC
TQTVAMTARQREFGPPRLLPRDAAARGAESLAARAGDEIAFVFGNERYGLPNDVVERCVA
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHH
VTHIPANPVYASLNLAQAVQLIAYEMRLALLDRAQAAPDDSGNIGYAGEPATAEQVESMF
HHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
GHLQQGLEAIGFLDPANPRKLMTRLRRLLARAGLEREEVNILRGIAKHMLIAVRGGRTKP
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEECCCCCC
EQSGPSTPARQEEH
CCCCCCCCCCCCCC
>Mature Secondary Structure
MLQASRWQAKYRRRAEEEAELYMNPANETSQAAGTAALAMPADGMFARVRFVLVETSHPG
CCCCHHHHHHHHHCCHHHHHEEECCCCCHHHHCCCEEEECCCCCCEEEEEEEEEECCCCC
NVGSVARAIKTMGFGSLVLVSPREPGVREHPDAVAMASGADDVLAGAVIVDSIEAALEGA
CHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCC
TQTVAMTARQREFGPPRLLPRDAAARGAESLAARAGDEIAFVFGNERYGLPNDVVERCVA
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHH
VTHIPANPVYASLNLAQAVQLIAYEMRLALLDRAQAAPDDSGNIGYAGEPATAEQVESMF
HHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
GHLQQGLEAIGFLDPANPRKLMTRLRRLLARAGLEREEVNILRGIAKHMLIAVRGGRTKP
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEECCCCCC
EQSGPSTPARQEEH
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA