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The map label for this gene is pyrG [H]

Identifier: 73540790

GI number: 73540790

Start: 1181826

End: 1183478

Strand: Direct

Name: pyrG [H]

Synonym: Reut_A1088

Alternate gene names: 73540790

Gene position: 1181826-1183478 (Clockwise)

Preceding gene: 73540789

Following gene: 73540791

Centisome position: 31.05

GC content: 63.46

Gene sequence:

>1653_bases
ATGACCAAATTCGTCTTCGTCACCGGTGGCGTCGTCTCTTCTCTCGGCAAGGGCATCGCTGCCGCCTCGCTCGCGGCTAT
TCTCGAGTCGCGCGGCCTCAAAGTCACCCTCCTCAAGCTAGATCCCTACATCAACGTCGATCCCGGCACGATGAGCCCCT
TCCAGCATGGCGAGGTGTTTGTCACGGAAGACGGTGCGGAAACCGACCTCGATCTTGGCCACTACGAGCGCTTCGTCTCG
GCCAAGATGCGCAAGTCGAACAACTTCACTACGGGCCAAATCTACGAATCGGTGATCCGCAAGGAGCGCCGCGGCGAATA
CCTGGGCAAGACCGTGCAGGTGATTCCGCATATCACCAACGAGATCCAGGCCTTTATCGAGCGTGGCGCTGCAGCGTCGC
ACGACGGCAAGGCTGATGTGGCGCTGGTGGAAATCGGCGGCACCGTCGGTGACATCGAATCGCTGCCGTTCCTGGAAGCC
GCGCGCCAGATGAGCCTGCGCATGGGCCGCAACCGCTGCGCGTTCGTGCACCTGACCCTGGTGCCGTTCATTGCCAGCGC
CGGCGAGCTGAAGACCAAGCCGACCCAGCACTCGGTGCAGAAGCTGCGTGAAATCGGCATTTCGCCGACCGCGCTGCTGT
GCCGCGCTGACCGTCCGATCCCGGACGACGAGCGCGCCAAGATTTCGCTGTTCGCCAACATCCCGCAAGACGCCGTGATT
TCGGTGTGGGATGCCGACAGCATCTACAAGATCCCGCAGATGCTCAACGAGCAGGGCCTGGACCGCCTGATCTGCGAAGA
GCTGCGCCTGGACCCGAAGCCGGCCGACCTGTCGATGTGGCAGAAGCTGGTGAACGCGCAGGAAAACCCCGAGCACGAAA
TCACCATCGGCATGGTCGGCAAGTACGTCGACCTGACCGAGTCGTACAAGTCGTTGATCGAGGCGCTGCGCCACGCCGGC
ATGCACACCGCCACGCGCGTGAACATCGAGTACATCGATTCCGAAGAACTCGAATCGGGCCATCTCGAAGTGCTGGCCCC
GCTGGACGCCATCCTGGTGCCGGGCGGTTTCGGCAAGCGCGGCACGGAAGGCAAGATCCGCGCGATCCAGTACGCCCGCG
AGAACAAGGTTCCGTACCTGGGCATCTGCCTGGGCATGCAGCTCGCCGTGATCGAGTTCGCCCGTCACGTGGCTGGCATG
ACCGACGCCAACTCGACCGAGTTCAACCTGGAAACCGAACATCCGGTGGTCGCGCTGATCACGGAGTGGGTGGACCGCGA
AGGCAAGGTCGAGCAGCGTTCGGCCGAATCCGACCTGGGCGGCACCATGCGCCTGGGTGCCCAACGCGTACCGGTGAAGG
AAGGGACCAAGGCGGCGTCGATCTACGGCGCGGAGGTCAACGAGCGTCACCGTCACCGCTACGAAGTCAACAACCACTAT
GTGCCGACGCTGGAAAACGCCGGCATGGTGATTTCGGCCCGTACCCCGACCGAGAACCTGCCCGAGATGATGGAACTGCC
GGGGTCGATGCACCCGTGGTTCGTCGGCGTGCAGTTCCACCCGGAATTCACCTCCACGCCGCGTGACGGCCATCCGCTGT
TCAAGGCCTACGTCGAAGCCGCGCTGGCTGGCCAGCAGCGCAAGGGCGCCTGA

Upstream 100 bases:

>100_bases
TGCGCCGCAGGCGTTGCGCAAGGCGGTGGTGTCGCAATTCGCGGCCGTTCGGGGGCCGGGGTCTCATGTATAATCCTAAT
TTCCCCGGCAAGCTCGGTTT

Downstream 100 bases:

>100_bases
AGCCGGCGCCGAGCAGGAGACTGTCATGAAACTCTGCGGATTCGAGGTTGGCCTCGACAAGCCGTTCTTTCTGATTGCCG
GCCCGTGCGTGATCGAGTCG

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 550; Mature: 549

Protein sequence:

>550_residues
MTKFVFVTGGVVSSLGKGIAAASLAAILESRGLKVTLLKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFVS
AKMRKSNNFTTGQIYESVIRKERRGEYLGKTVQVIPHITNEIQAFIERGAAASHDGKADVALVEIGGTVGDIESLPFLEA
ARQMSLRMGRNRCAFVHLTLVPFIASAGELKTKPTQHSVQKLREIGISPTALLCRADRPIPDDERAKISLFANIPQDAVI
SVWDADSIYKIPQMLNEQGLDRLICEELRLDPKPADLSMWQKLVNAQENPEHEITIGMVGKYVDLTESYKSLIEALRHAG
MHTATRVNIEYIDSEELESGHLEVLAPLDAILVPGGFGKRGTEGKIRAIQYARENKVPYLGICLGMQLAVIEFARHVAGM
TDANSTEFNLETEHPVVALITEWVDREGKVEQRSAESDLGGTMRLGAQRVPVKEGTKAASIYGAEVNERHRHRYEVNNHY
VPTLENAGMVISARTPTENLPEMMELPGSMHPWFVGVQFHPEFTSTPRDGHPLFKAYVEAALAGQQRKGA

Sequences:

>Translated_550_residues
MTKFVFVTGGVVSSLGKGIAAASLAAILESRGLKVTLLKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFVS
AKMRKSNNFTTGQIYESVIRKERRGEYLGKTVQVIPHITNEIQAFIERGAAASHDGKADVALVEIGGTVGDIESLPFLEA
ARQMSLRMGRNRCAFVHLTLVPFIASAGELKTKPTQHSVQKLREIGISPTALLCRADRPIPDDERAKISLFANIPQDAVI
SVWDADSIYKIPQMLNEQGLDRLICEELRLDPKPADLSMWQKLVNAQENPEHEITIGMVGKYVDLTESYKSLIEALRHAG
MHTATRVNIEYIDSEELESGHLEVLAPLDAILVPGGFGKRGTEGKIRAIQYARENKVPYLGICLGMQLAVIEFARHVAGM
TDANSTEFNLETEHPVVALITEWVDREGKVEQRSAESDLGGTMRLGAQRVPVKEGTKAASIYGAEVNERHRHRYEVNNHY
VPTLENAGMVISARTPTENLPEMMELPGSMHPWFVGVQFHPEFTSTPRDGHPLFKAYVEAALAGQQRKGA
>Mature_549_residues
TKFVFVTGGVVSSLGKGIAAASLAAILESRGLKVTLLKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFVSA
KMRKSNNFTTGQIYESVIRKERRGEYLGKTVQVIPHITNEIQAFIERGAAASHDGKADVALVEIGGTVGDIESLPFLEAA
RQMSLRMGRNRCAFVHLTLVPFIASAGELKTKPTQHSVQKLREIGISPTALLCRADRPIPDDERAKISLFANIPQDAVIS
VWDADSIYKIPQMLNEQGLDRLICEELRLDPKPADLSMWQKLVNAQENPEHEITIGMVGKYVDLTESYKSLIEALRHAGM
HTATRVNIEYIDSEELESGHLEVLAPLDAILVPGGFGKRGTEGKIRAIQYARENKVPYLGICLGMQLAVIEFARHVAGMT
DANSTEFNLETEHPVVALITEWVDREGKVEQRSAESDLGGTMRLGAQRVPVKEGTKAASIYGAEVNERHRHRYEVNNHYV
PTLENAGMVISARTPTENLPEMMELPGSMHPWFVGVQFHPEFTSTPRDGHPLFKAYVEAALAGQQRKGA

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=564, Percent_Identity=44.8581560283688, Blast_Score=473, Evalue=1e-133,
Organism=Homo sapiens, GI28559085, Length=559, Percent_Identity=43.649373881932, Blast_Score=468, Evalue=1e-132,
Organism=Homo sapiens, GI28559083, Length=559, Percent_Identity=43.649373881932, Blast_Score=468, Evalue=1e-132,
Organism=Homo sapiens, GI221316689, Length=559, Percent_Identity=43.649373881932, Blast_Score=468, Evalue=1e-132,
Organism=Escherichia coli, GI1789142, Length=548, Percent_Identity=61.8613138686131, Blast_Score=687, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25148299, Length=611, Percent_Identity=38.1342062193126, Blast_Score=415, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6319432, Length=570, Percent_Identity=41.0526315789474, Blast_Score=444, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6322563, Length=574, Percent_Identity=41.8118466898955, Blast_Score=439, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24664469, Length=560, Percent_Identity=44.8214285714286, Blast_Score=473, Evalue=1e-133,
Organism=Drosophila melanogaster, GI21357815, Length=505, Percent_Identity=43.5643564356436, Blast_Score=400, Evalue=1e-111,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 60684; Mature: 60553

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKFVFVTGGVVSSLGKGIAAASLAAILESRGLKVTLLKLDPYINVDPGTMSPFQHGEVF
CCEEEEEECHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCCCCCCCCEEE
VTEDGAETDLDLGHYERFVSAKMRKSNNFTTGQIYESVIRKERRGEYLGKTVQVIPHITN
EECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
EIQAFIERGAAASHDGKADVALVEIGGTVGDIESLPFLEAARQMSLRMGRNRCAFVHLTL
HHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEH
VPFIASAGELKTKPTQHSVQKLREIGISPTALLCRADRPIPDDERAKISLFANIPQDAVI
HHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCEE
SVWDADSIYKIPQMLNEQGLDRLICEELRLDPKPADLSMWQKLVNAQENPEHEITIGMVG
EEECCCHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCEEEEEECC
KYVDLTESYKSLIEALRHAGMHTATRVNIEYIDSEELESGHLEVLAPLDAILVPGGFGKR
CHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCEEEEECCCEEEECCCCCCC
GTEGKIRAIQYARENKVPYLGICLGMQLAVIEFARHVAGMTDANSTEFNLETEHPVVALI
CCCCCEEEEEEHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHH
TEWVDREGKVEQRSAESDLGGTMRLGAQRVPVKEGTKAASIYGAEVNERHRHRYEVNNHY
HHHHCCCCCCHHHHCCCCCCCHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHEECCCCC
VPTLENAGMVISARTPTENLPEMMELPGSMHPWFVGVQFHPEFTSTPRDGHPLFKAYVEA
CCCCCCCCEEEEECCCCCCCHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHH
ALAGQQRKGA
HHCCCCCCCC
>Mature Secondary Structure 
TKFVFVTGGVVSSLGKGIAAASLAAILESRGLKVTLLKLDPYINVDPGTMSPFQHGEVF
CEEEEEECHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCCCCCCCCEEE
VTEDGAETDLDLGHYERFVSAKMRKSNNFTTGQIYESVIRKERRGEYLGKTVQVIPHITN
EECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
EIQAFIERGAAASHDGKADVALVEIGGTVGDIESLPFLEAARQMSLRMGRNRCAFVHLTL
HHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEH
VPFIASAGELKTKPTQHSVQKLREIGISPTALLCRADRPIPDDERAKISLFANIPQDAVI
HHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCEE
SVWDADSIYKIPQMLNEQGLDRLICEELRLDPKPADLSMWQKLVNAQENPEHEITIGMVG
EEECCCHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCEEEEEECC
KYVDLTESYKSLIEALRHAGMHTATRVNIEYIDSEELESGHLEVLAPLDAILVPGGFGKR
CHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCEEEEECCCEEEECCCCCCC
GTEGKIRAIQYARENKVPYLGICLGMQLAVIEFARHVAGMTDANSTEFNLETEHPVVALI
CCCCCEEEEEEHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHH
TEWVDREGKVEQRSAESDLGGTMRLGAQRVPVKEGTKAASIYGAEVNERHRHRYEVNNHY
HHHHCCCCCCHHHHCCCCCCCHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHEECCCCC
VPTLENAGMVISARTPTENLPEMMELPGSMHPWFVGVQFHPEFTSTPRDGHPLFKAYVEA
CCCCCCCCEEEEECCCCCCCHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHH
ALAGQQRKGA
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]