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The map label for this gene is iscS [H]

Identifier: 73540760

GI number: 73540760

Start: 1150462

End: 1151679

Strand: Direct

Name: iscS [H]

Synonym: Reut_A1058

Alternate gene names: 73540760

Gene position: 1150462-1151679 (Clockwise)

Preceding gene: 73540759

Following gene: 73540761

Centisome position: 30.22

GC content: 63.22

Gene sequence:

>1218_bases
ATGAGCACCCCACATTTCCCCATCTACATGGATTACTCGGCCACCACGCCGGTGGACCCGCGCGTGGCGGACAAGATGAT
TCCGTACCTGCGCGAGCAGTTCGGCAATCCCGCGTCGCGCAGTCACGCATATGGCTGGGAAGCGGAGCGTGCGGTGGAAG
AAGCACGCGAGCAGGTGGCCGCGCTGGTCGGTGCCGATCCGCGTGAAATCGTGTGGACGTCTGGGGCGACCGAGTCGAAC
AACCTCGCGATCAAGGGCGCCGCGAACTTCTATTCGGGCAAGGGCAAGCACCTGATCACGGTGAAGACCGAGCACAAGGC
CGTGCTCGACACCACGCGCGAACTGGAGCGCCAGGGCTTCGAGGTGACCTACCTCGACGTGAAGGAAAACGGCCTGATCG
ACATGGATGTGCTCAAGCAGGCCATCCGCCCGGACACGATCCTGGTGTCGGTCATGTTCGTGAACAACGAGATCGGCGTG
ATCCAGGACATCGAGCAGATCGGCGAGATCTGCCGCGAGAAGGGCATCATCTTCCATTGCGACGCCGCGCAGGCGACCGG
CAAGGTGGTGATCGACCTCAACAAGCTGAAGGTCGACCTGATGTCGTTCTCGGCCCACAAGACTTATGGCCCGAAGGGTA
TCGGCGCGCTGTACGTGCGCCGCAAGCCGCGCGTCCGCATCGAGGCGCAGATGCACGGTGGCGGCCATGAGCGCGGCATG
CGCTCGGGCACGCTGGCCACGCACCAGATCGTCGGCATGGGCGAAGCGTTCCGTATCGCCCGCGAAGAAATGGCGACCGA
GAACGAACGCATCCGCATGCTGCGCGACCGCCTGTGGAATGGCCTGAAGAGCATGGAAGAGGTCTACCTGAACGGCGACC
TCGAACAGCGCGTGCCGCACAACCTGAACGTCAGCTTCAACTTCGTCGAAGGCGAGTCGCTGATCATGGCGATCAAGGAT
GTGGCCGTGTCGTCGGGCTCGGCCTGCACCTCGGCCTCGCTGGAGCCGTCGTACGTGCTGCGCGCACTGGGCCGCAATGA
CGAACTCGCGCACAGCTCGATCCGTTTCACGGTCGGCCGTTTCACGACCGAGCAGGAAGTCGACTACACCGTCGAACTGC
TCAAGAACAAGATCGGCAAGCTGCGCGACCTGTCGCCGCTGTGGGAGATGTTCAAGGACGGCGTCGACCTGAACTCGATC
CAGTGGGCCGCGCACTGA

Upstream 100 bases:

>100_bases
ATGGTGAATTCGGTTTTCAGCCTGGCGCAGTCCTGAAGCGCCCGGTAGCAAGCAGCGATACAAGCGATCAGCGGTCCCCC
TGAAAAAGGCAGTCATAACG

Downstream 100 bases:

>100_bases
GCGTTCGCCGGATCCCGCAAGAATAAAAGCAAGACCCCCGTAAAAGATACGCAAGAGGTAAGCAAAATGTCATACAGCAC
GAAGGTTCTCGACCACTATG

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MSTPHFPIYMDYSATTPVDPRVADKMIPYLREQFGNPASRSHAYGWEAERAVEEAREQVAALVGADPREIVWTSGATESN
NLAIKGAANFYSGKGKHLITVKTEHKAVLDTTRELERQGFEVTYLDVKENGLIDMDVLKQAIRPDTILVSVMFVNNEIGV
IQDIEQIGEICREKGIIFHCDAAQATGKVVIDLNKLKVDLMSFSAHKTYGPKGIGALYVRRKPRVRIEAQMHGGGHERGM
RSGTLATHQIVGMGEAFRIAREEMATENERIRMLRDRLWNGLKSMEEVYLNGDLEQRVPHNLNVSFNFVEGESLIMAIKD
VAVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTVGRFTTEQEVDYTVELLKNKIGKLRDLSPLWEMFKDGVDLNSI
QWAAH

Sequences:

>Translated_405_residues
MSTPHFPIYMDYSATTPVDPRVADKMIPYLREQFGNPASRSHAYGWEAERAVEEAREQVAALVGADPREIVWTSGATESN
NLAIKGAANFYSGKGKHLITVKTEHKAVLDTTRELERQGFEVTYLDVKENGLIDMDVLKQAIRPDTILVSVMFVNNEIGV
IQDIEQIGEICREKGIIFHCDAAQATGKVVIDLNKLKVDLMSFSAHKTYGPKGIGALYVRRKPRVRIEAQMHGGGHERGM
RSGTLATHQIVGMGEAFRIAREEMATENERIRMLRDRLWNGLKSMEEVYLNGDLEQRVPHNLNVSFNFVEGESLIMAIKD
VAVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTVGRFTTEQEVDYTVELLKNKIGKLRDLSPLWEMFKDGVDLNSI
QWAAH
>Mature_404_residues
STPHFPIYMDYSATTPVDPRVADKMIPYLREQFGNPASRSHAYGWEAERAVEEAREQVAALVGADPREIVWTSGATESNN
LAIKGAANFYSGKGKHLITVKTEHKAVLDTTRELERQGFEVTYLDVKENGLIDMDVLKQAIRPDTILVSVMFVNNEIGVI
QDIEQIGEICREKGIIFHCDAAQATGKVVIDLNKLKVDLMSFSAHKTYGPKGIGALYVRRKPRVRIEAQMHGGGHERGMR
SGTLATHQIVGMGEAFRIAREEMATENERIRMLRDRLWNGLKSMEEVYLNGDLEQRVPHNLNVSFNFVEGESLIMAIKDV
AVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTVGRFTTEQEVDYTVELLKNKIGKLRDLSPLWEMFKDGVDLNSIQ
WAAH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=400, Percent_Identity=62, Blast_Score=538, Evalue=1e-153,
Organism=Homo sapiens, GI156713448, Length=420, Percent_Identity=32.8571428571429, Blast_Score=210, Evalue=2e-54,
Organism=Escherichia coli, GI48994898, Length=402, Percent_Identity=76.3681592039801, Blast_Score=649, Evalue=0.0,
Organism=Escherichia coli, GI1789175, Length=229, Percent_Identity=29.2576419213974, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1787970, Length=214, Percent_Identity=26.1682242990654, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI25143064, Length=401, Percent_Identity=61.5960099750624, Blast_Score=526, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI17533177, Length=327, Percent_Identity=32.4159021406728, Blast_Score=146, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6319831, Length=399, Percent_Identity=58.6466165413534, Blast_Score=509, Evalue=1e-145,
Organism=Drosophila melanogaster, GI20129463, Length=400, Percent_Identity=58.25, Blast_Score=498, Evalue=1e-141,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 45324; Mature: 45193

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPHFPIYMDYSATTPVDPRVADKMIPYLREQFGNPASRSHAYGWEAERAVEEAREQVA
CCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
ALVGADPREIVWTSGATESNNLAIKGAANFYSGKGKHLITVKTEHKAVLDTTRELERQGF
HHHCCCCHHEEEECCCCCCCCEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCC
EVTYLDVKENGLIDMDVLKQAIRPDTILVSVMFVNNEIGVIQDIEQIGEICREKGIIFHC
EEEEEEECCCCEEEHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEE
DAAQATGKVVIDLNKLKVDLMSFSAHKTYGPKGIGALYVRRKPRVRIEAQMHGGGHERGM
CCCCCCCEEEEEEEHHEEEEHHCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCHHCCC
RSGTLATHQIVGMGEAFRIAREEMATENERIRMLRDRLWNGLKSMEEVYLNGDLEQRVPH
CCCCCHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCC
NLNVSFNFVEGESLIMAIKDVAVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTVGR
CCCEEEEEECCCEEEEEEEHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEEC
FTTEQEVDYTVELLKNKIGKLRDLSPLWEMFKDGVDLNSIQWAAH
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEECC
>Mature Secondary Structure 
STPHFPIYMDYSATTPVDPRVADKMIPYLREQFGNPASRSHAYGWEAERAVEEAREQVA
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
ALVGADPREIVWTSGATESNNLAIKGAANFYSGKGKHLITVKTEHKAVLDTTRELERQGF
HHHCCCCHHEEEECCCCCCCCEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCC
EVTYLDVKENGLIDMDVLKQAIRPDTILVSVMFVNNEIGVIQDIEQIGEICREKGIIFHC
EEEEEEECCCCEEEHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEE
DAAQATGKVVIDLNKLKVDLMSFSAHKTYGPKGIGALYVRRKPRVRIEAQMHGGGHERGM
CCCCCCCEEEEEEEHHEEEEHHCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCHHCCC
RSGTLATHQIVGMGEAFRIAREEMATENERIRMLRDRLWNGLKSMEEVYLNGDLEQRVPH
CCCCCHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCC
NLNVSFNFVEGESLIMAIKDVAVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTVGR
CCCEEEEEECCCEEEEEEEHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEEC
FTTEQEVDYTVELLKNKIGKLRDLSPLWEMFKDGVDLNSIQWAAH
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA