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The map label for this gene is grpE

Identifier: 73540743

GI number: 73540743

Start: 1134091

End: 1134645

Strand: Direct

Name: grpE

Synonym: Reut_A1040

Alternate gene names: 73540743

Gene position: 1134091-1134645 (Clockwise)

Preceding gene: 73540741

Following gene: 73540744

Centisome position: 29.79

GC content: 65.77

Gene sequence:

>555_bases
ATGGAAGAACAGAAGCAGACGCCATCCACCCCGACGCCTGACACGGCCGCCGAAGCGGCCGTCAACGCCGCAACGGCCGC
GCCCGAGACTGCCGGCGCGGATGACGTGGCGGCGCAGCTTGCCGCGCTGGAAGCTAAGGCGCGCGAGCACTACGACATGT
ACGTGCGTGCCGTCGCGGAGGGTGAAAACATCCGCCGCCGGGCACAGGAAGACGTGTCCAAGGCGCACAAGTTCGCCATC
GAGAATTTTGCCGACAATCTGCTGCCCGTGATGGACAGCCTGCAAGCCGCGCTCGCCGACGGCTCGGGCGATATCGCCAA
GCTGCGTGAAGGTGTCGAGTTGACCGCGCGCCAGCTGTCGGCAGCCTTCGAGCGCGGCAAGATCGTCGAACTGAACCCGG
TCGGCGAAAAGTTCGACCCGCATCGCCACCAGGCCATCTCGATGGTCCCGTCCGAGCAGGAGTCGAACACGGTCGTGACC
GTGCTGCAGCGCGGCTACACGATTGCGGATCGCGTGCTGCGTCCGGCGCTGGTGACAGTATCGGCCCCTAAGTAA

Upstream 100 bases:

>100_bases
TTGAAAACTCACGGTTTGGTACAACATCCTCGTGACACCGCCGATTTACCCACCGCGGCCCGCATTGGCCGGTCGCGACA
ATGTATTGATACGGATCGAC

Downstream 100 bases:

>100_bases
GCGGTAGCGGCGGGCGCGGCTGATGCGCCCGCAGGCTCTGCCCAACGACGCCCCAGCGCCATCCTGGCCCTGGGGCGTCT
TGCCATTCCGCGAGGTATGA

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MEEQKQTPSTPTPDTAAEAAVNAATAAPETAGADDVAAQLAALEAKAREHYDMYVRAVAEGENIRRRAQEDVSKAHKFAI
ENFADNLLPVMDSLQAALADGSGDIAKLREGVELTARQLSAAFERGKIVELNPVGEKFDPHRHQAISMVPSEQESNTVVT
VLQRGYTIADRVLRPALVTVSAPK

Sequences:

>Translated_184_residues
MEEQKQTPSTPTPDTAAEAAVNAATAAPETAGADDVAAQLAALEAKAREHYDMYVRAVAEGENIRRRAQEDVSKAHKFAI
ENFADNLLPVMDSLQAALADGSGDIAKLREGVELTARQLSAAFERGKIVELNPVGEKFDPHRHQAISMVPSEQESNTVVT
VLQRGYTIADRVLRPALVTVSAPK
>Mature_184_residues
MEEQKQTPSTPTPDTAAEAAVNAATAAPETAGADDVAAQLAALEAKAREHYDMYVRAVAEGENIRRRAQEDVSKAHKFAI
ENFADNLLPVMDSLQAALADGSGDIAKLREGVELTARQLSAAFERGKIVELNPVGEKFDPHRHQAISMVPSEQESNTVVT
VLQRGYTIADRVLRPALVTVSAPK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=147, Percent_Identity=38.0952380952381, Blast_Score=100, Evalue=8e-22,
Organism=Homo sapiens, GI40255109, Length=150, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=1e-15,
Organism=Escherichia coli, GI1788967, Length=173, Percent_Identity=39.3063583815029, Blast_Score=105, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17552458, Length=161, Percent_Identity=34.1614906832298, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324806, Length=173, Percent_Identity=32.3699421965318, Blast_Score=93, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24653432, Length=177, Percent_Identity=36.1581920903955, Blast_Score=101, Evalue=2e-22,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_CUPPJ (Q473L4)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_295263.1
- ProteinModelPortal:   Q473L4
- SMR:   Q473L4
- GeneID:   3610492
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A1040
- NMPDR:   fig|264198.3.peg.1255
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- ProtClustDB:   PRK14139
- BioCyc:   REUT264198:REUT_A1040-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 19773; Mature: 19773

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEQKQTPSTPTPDTAAEAAVNAATAAPETAGADDVAAQLAALEAKAREHYDMYVRAVAE
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GENIRRRAQEDVSKAHKFAIENFADNLLPVMDSLQAALADGSGDIAKLREGVELTARQLS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
AAFERGKIVELNPVGEKFDPHRHQAISMVPSEQESNTVVTVLQRGYTIADRVLRPALVTV
HHHHCCCEEEECCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHEEE
SAPK
ECCC
>Mature Secondary Structure
MEEQKQTPSTPTPDTAAEAAVNAATAAPETAGADDVAAQLAALEAKAREHYDMYVRAVAE
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GENIRRRAQEDVSKAHKFAIENFADNLLPVMDSLQAALADGSGDIAKLREGVELTARQLS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
AAFERGKIVELNPVGEKFDPHRHQAISMVPSEQESNTVVTVLQRGYTIADRVLRPALVTV
HHHHCCCEEEECCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHEEE
SAPK
ECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA