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The map label for this gene is gcvA [H]

Identifier: 73540722

GI number: 73540722

Start: 1107758

End: 1108651

Strand: Direct

Name: gcvA [H]

Synonym: Reut_A1019

Alternate gene names: 73540722

Gene position: 1107758-1108651 (Clockwise)

Preceding gene: 73540718

Following gene: 73540723

Centisome position: 29.1

GC content: 65.88

Gene sequence:

>894_bases
ATGATGCCGTCGATGACCGAATTGCAGGCGTTTGAAGCGTCGGCCAGGCACAGCAGCTTTACCGCGGCCGCGCGGGCGCT
GCATGTGACGCAGGGGGCGGTGAGCAAGCAGGTGCGCAGCCTGGAGTCTCTTCTCGGCGTAGAGTTGTTCGAACGCGTGC
GCCAGCGGCTAATACTGACGCCGGCCGGCGTGCGCTATCTGGAACGAGTCCGGCCCACGCTCGACGAGATCGAGGCGGCC
ACGGTAGAAGCGGCGGGACGAGAACAACAGGCGGGGACGCTGCATATCGCGAGCATGCCGACGCTCGGCGCCAAATGGCT
GATCCCGCGCTTGCCCCAGTTCTTTGCGCGGCATCCCGAAGTCCGGCTCGAGTTTGTCCCGCACGCGCAGGGCTATGTTT
TTGCCGAGCCCGAACTGGACGCGGCGATCCGCTTTGGCGTGGGCGTCTGGCCCGGGAGTCTGGCCGACTACATGATCGGC
CGCGAAATCGTGCCTGTGTGCCGGCCGGCTTTGGTGGCCGGCCATCCGGGCGGCGTGGTCGGCGCGCCCGCAGAGCTGCA
GCAGTATCCATTGCTGCACCACACGACAGTGCCGGAAGCCTGGCCCGACTGGTTCGCTGAACTTGGCGTGCCGACGCGCG
ATGCCTGGGCCGGGGCGCGTTTCGACCAGTTTTCGCTGCTCACGCAAGCGGCGCTATCGGGTCTTGGCATTGCGCTGATT
CCACGTTGCCTGATCGAGGAAGAGCTGGCCAACGGCGCACTGGTGGTCGCGCATCCGGCGCGCGTGCTCAGCAACAAGGG
CTATTACCTGTGCTACCCCGAGCACAAGCAACATCTGCCTGCCTTGCAGACTTTCCGTGCCTGGCTGATGGAGGGTACCG
ACACGCGCGGCTGA

Upstream 100 bases:

>100_bases
GTTTCCCCGATTCTGCTCGCGCCGGCAAGATGCCCGCAAACGAAATAAAATCGCACGGTCATTCCATTTGGTCGTGAAGT
TGAGGTGATGTATGCGGCGC

Downstream 100 bases:

>100_bases
CTTTGTTGCCTCGCGGCCGCAACAGACAGGGTTTGCCGCCATGTTGCGCGCCTGAGCCGTCGCGGACACTGCCCCGCAAA
AGAACCGAGGCGGGGTCAAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MMPSMTELQAFEASARHSSFTAAARALHVTQGAVSKQVRSLESLLGVELFERVRQRLILTPAGVRYLERVRPTLDEIEAA
TVEAAGREQQAGTLHIASMPTLGAKWLIPRLPQFFARHPEVRLEFVPHAQGYVFAEPELDAAIRFGVGVWPGSLADYMIG
REIVPVCRPALVAGHPGGVVGAPAELQQYPLLHHTTVPEAWPDWFAELGVPTRDAWAGARFDQFSLLTQAALSGLGIALI
PRCLIEEELANGALVVAHPARVLSNKGYYLCYPEHKQHLPALQTFRAWLMEGTDTRG

Sequences:

>Translated_297_residues
MMPSMTELQAFEASARHSSFTAAARALHVTQGAVSKQVRSLESLLGVELFERVRQRLILTPAGVRYLERVRPTLDEIEAA
TVEAAGREQQAGTLHIASMPTLGAKWLIPRLPQFFARHPEVRLEFVPHAQGYVFAEPELDAAIRFGVGVWPGSLADYMIG
REIVPVCRPALVAGHPGGVVGAPAELQQYPLLHHTTVPEAWPDWFAELGVPTRDAWAGARFDQFSLLTQAALSGLGIALI
PRCLIEEELANGALVVAHPARVLSNKGYYLCYPEHKQHLPALQTFRAWLMEGTDTRG
>Mature_297_residues
MMPSMTELQAFEASARHSSFTAAARALHVTQGAVSKQVRSLESLLGVELFERVRQRLILTPAGVRYLERVRPTLDEIEAA
TVEAAGREQQAGTLHIASMPTLGAKWLIPRLPQFFARHPEVRLEFVPHAQGYVFAEPELDAAIRFGVGVWPGSLADYMIG
REIVPVCRPALVAGHPGGVVGAPAELQQYPLLHHTTVPEAWPDWFAELGVPTRDAWAGARFDQFSLLTQAALSGLGIALI
PRCLIEEELANGALVVAHPARVLSNKGYYLCYPEHKQHLPALQTFRAWLMEGTDTRG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=291, Percent_Identity=31.2714776632302, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1786448, Length=286, Percent_Identity=29.3706293706294, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1788706, Length=300, Percent_Identity=28, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI145693193, Length=306, Percent_Identity=27.4509803921569, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1786401, Length=269, Percent_Identity=30.4832713754647, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787589, Length=298, Percent_Identity=26.1744966442953, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787128, Length=297, Percent_Identity=25.9259259259259, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI157672245, Length=145, Percent_Identity=36.551724137931, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1789204, Length=128, Percent_Identity=36.71875, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787806, Length=255, Percent_Identity=27.0588235294118, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790399, Length=118, Percent_Identity=35.5932203389831, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789639, Length=282, Percent_Identity=26.5957446808511, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788887, Length=119, Percent_Identity=34.453781512605, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787601, Length=285, Percent_Identity=23.859649122807, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32621; Mature: 32621

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPSMTELQAFEASARHSSFTAAARALHVTQGAVSKQVRSLESLLGVELFERVRQRLILT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PAGVRYLERVRPTLDEIEAATVEAAGREQQAGTLHIASMPTLGAKWLIPRLPQFFARHPE
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCC
VRLEFVPHAQGYVFAEPELDAAIRFGVGVWPGSLADYMIGREIVPVCRPALVAGHPGGVV
EEEEEECCCCCEEEECCCCCHHHHHCCCCCCCHHHHHHHCCHHHHHHCHHHCCCCCCCCC
GAPAELQQYPLLHHTTVPEAWPDWFAELGVPTRDAWAGARFDQFSLLTQAALSGLGIALI
CCCHHHHHCCCHHCCCCCCHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHCCCHHHH
PRCLIEEELANGALVVAHPARVLSNKGYYLCYPEHKQHLPALQTFRAWLMEGTDTRG
HHHHHHHHHCCCCEEEECCHHHHCCCCEEEEECCHHHHCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MMPSMTELQAFEASARHSSFTAAARALHVTQGAVSKQVRSLESLLGVELFERVRQRLILT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PAGVRYLERVRPTLDEIEAATVEAAGREQQAGTLHIASMPTLGAKWLIPRLPQFFARHPE
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCC
VRLEFVPHAQGYVFAEPELDAAIRFGVGVWPGSLADYMIGREIVPVCRPALVAGHPGGVV
EEEEEECCCCCEEEECCCCCHHHHHCCCCCCCHHHHHHHCCHHHHHHCHHHCCCCCCCCC
GAPAELQQYPLLHHTTVPEAWPDWFAELGVPTRDAWAGARFDQFSLLTQAALSGLGIALI
CCCHHHHHCCCHHCCCCCCHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHCCCHHHH
PRCLIEEELANGALVVAHPARVLSNKGYYLCYPEHKQHLPALQTFRAWLMEGTDTRG
HHHHHHHHHCCCCEEEECCHHHHCCCCEEEEECCHHHHCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]