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The map label for this gene is nuoM [H]

Identifier: 73540676

GI number: 73540676

Start: 1061389

End: 1062855

Strand: Direct

Name: nuoM [H]

Synonym: Reut_A0973

Alternate gene names: 73540676

Gene position: 1061389-1062855 (Clockwise)

Preceding gene: 73540675

Following gene: 73540677

Centisome position: 27.88

GC content: 60.53

Gene sequence:

>1467_bases
ATGGTTCTGTCTTTCGCAATCTGGCTGCCTATCTTCTTCGGCTTGCTGGTACTGGCCTCCGGCTCGGACCGCAGCGCCGG
CTACGTGAGGTGGATGTCGCTGTTCGGCTCGATCGCCAGCTTCATCGTTACGCTGCCCCTGGTGTTCCACTTCGACAAAT
CCACGGCGGCGATGCAGTTCGTGGAGAAGTCGTCCTGGATCGAACGCTTCAATATCCAGTACTACCTGGGCGTTGACGGC
ATCTCGATGTGGTTCGTCGTGCTGACGGCCTTCATCACCGTGATCGTCGTGATTTCGGCCTGGGAAGTGATCACCGAACG
CGTGGCCGAGTACATGGCTTCGTTCCTGATCCTGTCTGGCGTGATGGTCGGCGTGTTCTGCGCGCTGGACGGCATGCTGT
TCTACGTGTTCTTCGAAGCCACGCTGATCCCGATGTACATCATCATCGGCGTGTGGGGCGGTCCGAACCGTGTGTACGCG
GCCTTCAAGTTCTTCCTGTACACGCTGCTGGGCTCGCTGCTGACGCTGGTCGCGCTGCTGTACCTGTACAGCAAGACCGG
TACTTTCGAGATCCTGCAGTGGCACCAGGCCAAGCTGTCGCTGAACGAGCAGATCGCGCTGTTCATCGCTTTCTTCATGG
CTTTCGCCGTCAAGGTTCCGATGTGGCCGGTGCACACCTGGCTGCCCGATGCCCACGTGGAAGCGCCGACCGGCGGTTCC
GTCGTGCTGGCCGCGATCATGCTGAAGCTCGGTGCCTACGGTTTCCTGCGGTTCTCGCTGCCGATCGCGCCTGACGCAAG
CCATTCGCTGGCGGCGTTCATCATCACCATCTCGCTGATCGCCGTGATCTATATCGGCCTGGTGGCGCTGGTTCAGGCCG
ATATGAAGAAGCTGGTCGCGTATTCGTCGATCGCGCACATGGGTTTTGTAACGCTCGGTTTCTTCATCTTCAACGAAATC
GGCATCGAAGGCGGCATCGTGCAGATGATCTCGCACGGCTTCATCTCGGGCGCCATGTTCCTGTGCATCGGCGTGCTGTA
CGACCGCGTGCACTCGCGCCAGATTGCCGACTACGGCGGCGTGGTGAACACCATGCCGAAGTTCGCCGCGCTGTCGGTGT
TCTTCGCGATGGCCAACTGCGGCCTGCCGGCGACCTCTGGTTTCGTGGGCGAGTTCATGGTGATCCTGGGCTCGGTCGAG
TTCAACTTCTGGATCGGCCTGCTTGCCGCCACTGCACTGATCTTCGGCGCCGCCTACTCGCTGTGGATGGTCAAGCGCGT
GATCTTCGGCGACATCGTGCACCAGCACGTCCGCGAGCTGGTCGACCTGAACAAGCGCGAGTTCCTGATGCTGGGCCTTC
TCGCCATCATGACGCTGTACATGGGCCTGCACCCGAAGCCCTTCACCGACGTGATGCACGCTTCGGTGGTCAACCTGCTG
TCCCACGTGGCGCAGTCGAAGCTGTAA

Upstream 100 bases:

>100_bases
CGCATTCGCGATGATCGTCGGCATGCTGGTGCTGCTGACGCTCACCGTCACGGGCGTGATTGGCACCAAGTGATAGGCAC
CGAGTAAGGAAAACAAAGAA

Downstream 100 bases:

>100_bases
GCGGGGAGAGATAACACTATGCAACCGTCCTCGAGTCTAGCCCCTGTGGCGCCGATTATTTTCCTTGCGATCGCGATTGC
CGCGGTCAACTGGATCGACC

Product: NADH dehydrogenase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MVLSFAIWLPIFFGLLVLASGSDRSAGYVRWMSLFGSIASFIVTLPLVFHFDKSTAAMQFVEKSSWIERFNIQYYLGVDG
ISMWFVVLTAFITVIVVISAWEVITERVAEYMASFLILSGVMVGVFCALDGMLFYVFFEATLIPMYIIIGVWGGPNRVYA
AFKFFLYTLLGSLLTLVALLYLYSKTGTFEILQWHQAKLSLNEQIALFIAFFMAFAVKVPMWPVHTWLPDAHVEAPTGGS
VVLAAIMLKLGAYGFLRFSLPIAPDASHSLAAFIITISLIAVIYIGLVALVQADMKKLVAYSSIAHMGFVTLGFFIFNEI
GIEGGIVQMISHGFISGAMFLCIGVLYDRVHSRQIADYGGVVNTMPKFAALSVFFAMANCGLPATSGFVGEFMVILGSVE
FNFWIGLLAATALIFGAAYSLWMVKRVIFGDIVHQHVRELVDLNKREFLMLGLLAIMTLYMGLHPKPFTDVMHASVVNLL
SHVAQSKL

Sequences:

>Translated_488_residues
MVLSFAIWLPIFFGLLVLASGSDRSAGYVRWMSLFGSIASFIVTLPLVFHFDKSTAAMQFVEKSSWIERFNIQYYLGVDG
ISMWFVVLTAFITVIVVISAWEVITERVAEYMASFLILSGVMVGVFCALDGMLFYVFFEATLIPMYIIIGVWGGPNRVYA
AFKFFLYTLLGSLLTLVALLYLYSKTGTFEILQWHQAKLSLNEQIALFIAFFMAFAVKVPMWPVHTWLPDAHVEAPTGGS
VVLAAIMLKLGAYGFLRFSLPIAPDASHSLAAFIITISLIAVIYIGLVALVQADMKKLVAYSSIAHMGFVTLGFFIFNEI
GIEGGIVQMISHGFISGAMFLCIGVLYDRVHSRQIADYGGVVNTMPKFAALSVFFAMANCGLPATSGFVGEFMVILGSVE
FNFWIGLLAATALIFGAAYSLWMVKRVIFGDIVHQHVRELVDLNKREFLMLGLLAIMTLYMGLHPKPFTDVMHASVVNLL
SHVAQSKL
>Mature_488_residues
MVLSFAIWLPIFFGLLVLASGSDRSAGYVRWMSLFGSIASFIVTLPLVFHFDKSTAAMQFVEKSSWIERFNIQYYLGVDG
ISMWFVVLTAFITVIVVISAWEVITERVAEYMASFLILSGVMVGVFCALDGMLFYVFFEATLIPMYIIIGVWGGPNRVYA
AFKFFLYTLLGSLLTLVALLYLYSKTGTFEILQWHQAKLSLNEQIALFIAFFMAFAVKVPMWPVHTWLPDAHVEAPTGGS
VVLAAIMLKLGAYGFLRFSLPIAPDASHSLAAFIITISLIAVIYIGLVALVQADMKKLVAYSSIAHMGFVTLGFFIFNEI
GIEGGIVQMISHGFISGAMFLCIGVLYDRVHSRQIADYGGVVNTMPKFAALSVFFAMANCGLPATSGFVGEFMVILGSVE
FNFWIGLLAATALIFGAAYSLWMVKRVIFGDIVHQHVRELVDLNKREFLMLGLLAIMTLYMGLHPKPFTDVMHASVVNLL
SHVAQSKL

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=346, Percent_Identity=35.5491329479769, Blast_Score=199, Evalue=5e-51,
Organism=Homo sapiens, GI251831117, Length=384, Percent_Identity=23.9583333333333, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI1788613, Length=426, Percent_Identity=39.4366197183099, Blast_Score=310, Evalue=2e-85,
Organism=Escherichia coli, GI1788827, Length=284, Percent_Identity=24.2957746478873, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788829, Length=469, Percent_Identity=27.0788912579957, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1788831, Length=357, Percent_Identity=24.9299719887955, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI145693160, Length=424, Percent_Identity=24.0566037735849, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1788614, Length=345, Percent_Identity=24.0579710144928, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI2367154, Length=244, Percent_Identity=25.8196721311475, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 53956; Mature: 53956

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCC
>Mature Secondary Structure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HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8566820 [H]