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The map label for this gene is pmbA [H]

Identifier: 73540614

GI number: 73540614

Start: 992225

End: 993607

Strand: Direct

Name: pmbA [H]

Synonym: Reut_A0911

Alternate gene names: 73540614

Gene position: 992225-993607 (Clockwise)

Preceding gene: 73540611

Following gene: 73540620

Centisome position: 26.07

GC content: 66.81

Gene sequence:

>1383_bases
ATGACACCGCCCAGCATGGACTCCATCGCCGAACAGACCGCGCATTTCACCCATACCCAGGCCCAGCTCAGCGAGATGGC
CGCCGATGTGCTGCGTGTGGCACGCGAGCTTGGGGCGACCGATGCCGCGACCGAGATCTCGGAAGGCAGCGGTCTGTCGG
TGTCGGTGCGAAAGGGGCAGGTCGAGACCATCGAGCAGAACCGCGACAAGGTGGTCGGCGTCACGGTGCTGATCGGCAAG
CGCCGCGGCAACGCCAGCACGTCGGATTTCTCGCCGGCCGCGCTGCGTGCCACGGCCGAGGCGGCCTACAACATCGCCCG
CTTCACGGCTGAAGACGATTGCGCCGGCCTGGCCGAGGAAGAGCTGCTGGAGCGTAACCCGCAAGACCTCGACCTGTTCC
ATCCGTGGACGGTCGATGCCGAGGGCGCCATCGACATCGCGCGCCGGGCCGAAGCGGCCGCGTTCGCCGTATCGCCGCGC
ATCAAGAACAGCGATGGCGCCAGCGTATCGGCGCAGCATTCGCAGTTCGTGCTCGCGACCACGCGCGGTTTCATCGGCGG
CTATCCGTACTCGCGCCATTTCATCTCGTGCGCGCCGATTGCCGGCAGCGGCGGCGGCATGCAGCGCGACGACTGGTACT
CGTCCAAGCGCTCGCCGCTGGCGCTGGCCGTGCCCGAGGACATCGGCCGCTATGCCGCTGAACGCGCGCTGGCCCGCCTG
AATGCGCGCAAGCTGTCCACGCGCCGCTGCCCCGTGCTGTTCGAGGCGCCGCTGGCCGCCGGCCTGCTCGGCGCATTCGT
GCAGGCGGTTTCCGGTGGCGCGCTGTACCGCAAGTCCACCTTCCTGCATGACACGCTGGGCAAGGCGGTTTTCGCTCCGC
ATATCCAGATCCACGAGCGTCCGCACGTGCCGGGTGCCATGGGCAGCGCGCCGTTCGACGAAGAGGGTGTGCGCACGCGC
GAACGCGATGTCGTGCGCGACGGCGTGGTGGAGGGTTACTTCCTGTCGACCTACTCGGCACGCAAGCTTGGCATGAAGAC
CACCGGCAACGCGGGTGGTTCGCACAACCTGACGCTGACGAGCGATCTCACTACGGCCAGCGACGATTTCCGCGGCATGC
TCAAGAAGCTGGGCACCGGCCTGCTGGTGACGGAGCTGATGGGGCAGGGCGTGAACTACGTGACCGGCGACTATTCGCGC
GGCGCGTCGGGCTACTGGGTCGAGAACGGCGAGATCCAGTATCCGGTGGAAGAAATCACCATCGCCGGCAATATGGGCGA
GATGTTCCGCCAGATCGTTGCCATCGGCGCGGATTCGCTGGTGCGCGGTACCAAGGAAACCGGCTCCATCCTGATCGAAC
AGATGACCATCGCCGGCAACTGA

Upstream 100 bases:

>100_bases
CCGGCGCAAAGGCGCCAGGAAAGCGGGAGCCGGGGGAATTACGGGAATTTCGCGTCATGGGCTGCATTTTATCTTGCTAT
GATTCCCGCTTGGACTTTTG

Downstream 100 bases:

>100_bases
TCGCGTACGTCGCAAGCCAGTGAAAAGAAAGACCGCCCGAAAAACGAGCGGTCTTTTTTTTGTCCCTGGCGTACGGCGGC
CGGGCCGGGCTTGGGGCGGA

Product: microcin-processing peptidase 1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MTPPSMDSIAEQTAHFTHTQAQLSEMAADVLRVARELGATDAATEISEGSGLSVSVRKGQVETIEQNRDKVVGVTVLIGK
RRGNASTSDFSPAALRATAEAAYNIARFTAEDDCAGLAEEELLERNPQDLDLFHPWTVDAEGAIDIARRAEAAAFAVSPR
IKNSDGASVSAQHSQFVLATTRGFIGGYPYSRHFISCAPIAGSGGGMQRDDWYSSKRSPLALAVPEDIGRYAAERALARL
NARKLSTRRCPVLFEAPLAAGLLGAFVQAVSGGALYRKSTFLHDTLGKAVFAPHIQIHERPHVPGAMGSAPFDEEGVRTR
ERDVVRDGVVEGYFLSTYSARKLGMKTTGNAGGSHNLTLTSDLTTASDDFRGMLKKLGTGLLVTELMGQGVNYVTGDYSR
GASGYWVENGEIQYPVEEITIAGNMGEMFRQIVAIGADSLVRGTKETGSILIEQMTIAGN

Sequences:

>Translated_460_residues
MTPPSMDSIAEQTAHFTHTQAQLSEMAADVLRVARELGATDAATEISEGSGLSVSVRKGQVETIEQNRDKVVGVTVLIGK
RRGNASTSDFSPAALRATAEAAYNIARFTAEDDCAGLAEEELLERNPQDLDLFHPWTVDAEGAIDIARRAEAAAFAVSPR
IKNSDGASVSAQHSQFVLATTRGFIGGYPYSRHFISCAPIAGSGGGMQRDDWYSSKRSPLALAVPEDIGRYAAERALARL
NARKLSTRRCPVLFEAPLAAGLLGAFVQAVSGGALYRKSTFLHDTLGKAVFAPHIQIHERPHVPGAMGSAPFDEEGVRTR
ERDVVRDGVVEGYFLSTYSARKLGMKTTGNAGGSHNLTLTSDLTTASDDFRGMLKKLGTGLLVTELMGQGVNYVTGDYSR
GASGYWVENGEIQYPVEEITIAGNMGEMFRQIVAIGADSLVRGTKETGSILIEQMTIAGN
>Mature_459_residues
TPPSMDSIAEQTAHFTHTQAQLSEMAADVLRVARELGATDAATEISEGSGLSVSVRKGQVETIEQNRDKVVGVTVLIGKR
RGNASTSDFSPAALRATAEAAYNIARFTAEDDCAGLAEEELLERNPQDLDLFHPWTVDAEGAIDIARRAEAAAFAVSPRI
KNSDGASVSAQHSQFVLATTRGFIGGYPYSRHFISCAPIAGSGGGMQRDDWYSSKRSPLALAVPEDIGRYAAERALARLN
ARKLSTRRCPVLFEAPLAAGLLGAFVQAVSGGALYRKSTFLHDTLGKAVFAPHIQIHERPHVPGAMGSAPFDEEGVRTRE
RDVVRDGVVEGYFLSTYSARKLGMKTTGNAGGSHNLTLTSDLTTASDDFRGMLKKLGTGLLVTELMGQGVNYVTGDYSRG
ASGYWVENGEIQYPVEEITIAGNMGEMFRQIVAIGADSLVRGTKETGSILIEQMTIAGN

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=439, Percent_Identity=47.380410022779, Blast_Score=408, Evalue=1e-115,
Organism=Escherichia coli, GI1789640, Length=408, Percent_Identity=22.3039215686275, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 49178; Mature: 49047

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPPSMDSIAEQTAHFTHTQAQLSEMAADVLRVARELGATDAATEISEGSGLSVSVRKGQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEECCC
VETIEQNRDKVVGVTVLIGKRRGNASTSDFSPAALRATAEAAYNIARFTAEDDCAGLAEE
HHHHHCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHCCCHHH
ELLERNPQDLDLFHPWTVDAEGAIDIARRAEAAAFAVSPRIKNSDGASVSAQHSQFVLAT
HHHHCCCCCCEEECCEECCCCCHHHHHHHHHHHHEEECCCCCCCCCCCEECCCCEEEEEE
TRGFIGGYPYSRHFISCAPIAGSGGGMQRDDWYSSKRSPLALAVPEDIGRYAAERALARL
CCHHHCCCCCCCCEEEEECCCCCCCCCCCCHHHCCCCCCEEEECCHHHHHHHHHHHHHHH
NARKLSTRRCPVLFEAPLAAGLLGAFVQAVSGGALYRKSTFLHDTLGKAVFAPHIQIHER
HHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECC
PHVPGAMGSAPFDEEGVRTRERDVVRDGVVEGYFLSTYSARKLGMKTTGNAGGSHNLTLT
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCHHHHCCCCCCCCCCCCCCEEEE
SDLTTASDDFRGMLKKLGTGLLVTELMGQGVNYVTGDYSRGASGYWVENGEIQYPVEEIT
CCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCEEECCCCCCCCCEEEECCEEECCHHHEE
IAGNMGEMFRQIVAIGADSLVRGTKETGSILIEQMTIAGN
EECCHHHHHHHHHHHCCHHHHCCCCHHHHEEEEEEEECCC
>Mature Secondary Structure 
TPPSMDSIAEQTAHFTHTQAQLSEMAADVLRVARELGATDAATEISEGSGLSVSVRKGQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEECCC
VETIEQNRDKVVGVTVLIGKRRGNASTSDFSPAALRATAEAAYNIARFTAEDDCAGLAEE
HHHHHCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHCCCHHH
ELLERNPQDLDLFHPWTVDAEGAIDIARRAEAAAFAVSPRIKNSDGASVSAQHSQFVLAT
HHHHCCCCCCEEECCEECCCCCHHHHHHHHHHHHEEECCCCCCCCCCCEECCCCEEEEEE
TRGFIGGYPYSRHFISCAPIAGSGGGMQRDDWYSSKRSPLALAVPEDIGRYAAERALARL
CCHHHCCCCCCCCEEEEECCCCCCCCCCCCHHHCCCCCCEEEECCHHHHHHHHHHHHHHH
NARKLSTRRCPVLFEAPLAAGLLGAFVQAVSGGALYRKSTFLHDTLGKAVFAPHIQIHER
HHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECC
PHVPGAMGSAPFDEEGVRTRERDVVRDGVVEGYFLSTYSARKLGMKTTGNAGGSHNLTLT
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCHHHHCCCCCCCCCCCCCCEEEE
SDLTTASDDFRGMLKKLGTGLLVTELMGQGVNYVTGDYSRGASGYWVENGEIQYPVEEIT
CCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCEEECCCCCCCCCEEEECCEEECCHHHEE
IAGNMGEMFRQIVAIGADSLVRGTKETGSILIEQMTIAGN
EECCHHHHHHHHHHHCCHHHHCCCCHHHHEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]