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The map label for this gene is yneJ [H]

Identifier: 73540557

GI number: 73540557

Start: 926090

End: 926983

Strand: Reverse

Name: yneJ [H]

Synonym: Reut_A0854

Alternate gene names: 73540557

Gene position: 926983-926090 (Counterclockwise)

Preceding gene: 73540565

Following gene: 73540556

Centisome position: 24.35

GC content: 69.8

Gene sequence:

>894_bases
ATGGACCTGGCCACGCTTGAAATCTTCCGGACGGTGGTGGAGGCAGGCGGCGTCACACGCGCCGCAGAACGTCTGCACCG
GGTGCAATCCAACGTCACAACGCGCATCCGCCAGCTCGAGGAATCACTTGGCGTGGAGCTGTTCGTGCGTGACGGCAAGC
GCATGGTGCTGACGCCGGCCGGGCAGACACTGCTCGATTACGCCGGCCGTATGCTGCGGCTGGCCGACGAGGCGCGCCAT
GCGGTGCTGCCATCCCGCCCGCACGGTCGGCTGCGCATCGCATCGATGGAAAGCACTGCGGCCAGCCGGCTGCCCGCGAT
GCTGGCCGCCTATCACCAGGCCTGGTCGGAGGTCCAGCTCGAACTGGTCACATTGCCGACCCGGCAGGCCATGGAGGCGC
TGGAGCGCTTCGAGGCCGACTGCGCCTTCGTCGCCGAGCCGCTTGGCAATCCCGTGCTGGCATCGCGGCCGGCGTTCGAG
GAAGAACTGGTGGTGATCGCCCGCGCCGGACATCGCCCGATCCACAGTGCCGCGGATCTCGAAACTGGCACGCTGCTCGG
CTTCGAGCCTGGCTGCAATTACCGGGCCCGGCTTGAAGCGTGGCATGCGGCCGGCGGCGGACCGGCGCCGCGCGTGCTGG
AGCTGAATTCCTATCACGCAATCGTGGCCTGCGCGGCGGCAGGCATCGGCGCCGCAATCGTGCCGCGCTCGGTGCTCGGC
CTGTATCGCGACTTGCCTGCCATCAGCCTGCACAGTCTTGGCGAGGCGGGGTGCGTGCGAACGCTGCTGGCGTGGCATCC
GGCCATGACCAGCGCGGCGCTCGACGCGCTGGTTCAGGCGCTGCCAACGCCGGCCGCTCCGGCCGGCAACCCCGCCCTGC
CTGCCGCCGCCTGA

Upstream 100 bases:

>100_bases
TCAACTATGCTACGCCGTGATAATCATCTTCATAAATGAATTATCAAGATGCCATACATCTTTGATAGAGATGATACAAC
TCTGACTGACTGGAGCCGCC

Downstream 100 bases:

>100_bases
GCGCCCCATTCGCCGCGGGCCGTGCAGGATGTGCCCGGTCGCCTACACTGGAAGTGGCGCCACCATGGCACTCGGTGTGC
CACGCCCCTTGCGATGAGAT

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MDLATLEIFRTVVEAGGVTRAAERLHRVQSNVTTRIRQLEESLGVELFVRDGKRMVLTPAGQTLLDYAGRMLRLADEARH
AVLPSRPHGRLRIASMESTAASRLPAMLAAYHQAWSEVQLELVTLPTRQAMEALERFEADCAFVAEPLGNPVLASRPAFE
EELVVIARAGHRPIHSAADLETGTLLGFEPGCNYRARLEAWHAAGGGPAPRVLELNSYHAIVACAAAGIGAAIVPRSVLG
LYRDLPAISLHSLGEAGCVRTLLAWHPAMTSAALDALVQALPTPAAPAGNPALPAAA

Sequences:

>Translated_297_residues
MDLATLEIFRTVVEAGGVTRAAERLHRVQSNVTTRIRQLEESLGVELFVRDGKRMVLTPAGQTLLDYAGRMLRLADEARH
AVLPSRPHGRLRIASMESTAASRLPAMLAAYHQAWSEVQLELVTLPTRQAMEALERFEADCAFVAEPLGNPVLASRPAFE
EELVVIARAGHRPIHSAADLETGTLLGFEPGCNYRARLEAWHAAGGGPAPRVLELNSYHAIVACAAAGIGAAIVPRSVLG
LYRDLPAISLHSLGEAGCVRTLLAWHPAMTSAALDALVQALPTPAAPAGNPALPAAA
>Mature_297_residues
MDLATLEIFRTVVEAGGVTRAAERLHRVQSNVTTRIRQLEESLGVELFVRDGKRMVLTPAGQTLLDYAGRMLRLADEARH
AVLPSRPHGRLRIASMESTAASRLPAMLAAYHQAWSEVQLELVTLPTRQAMEALERFEADCAFVAEPLGNPVLASRPAFE
EELVVIARAGHRPIHSAADLETGTLLGFEPGCNYRARLEAWHAAGGGPAPRVLELNSYHAIVACAAAGIGAAIVPRSVLG
LYRDLPAISLHSLGEAGCVRTLLAWHPAMTSAALDALVQALPTPAAPAGNPALPAAA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=285, Percent_Identity=36.4912280701754, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI87082132, Length=243, Percent_Identity=32.9218106995885, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1788887, Length=291, Percent_Identity=27.4914089347079, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788296, Length=244, Percent_Identity=29.9180327868852, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI157672245, Length=254, Percent_Identity=29.1338582677165, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788481, Length=167, Percent_Identity=32.9341317365269, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1790262, Length=261, Percent_Identity=25.2873563218391, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790208, Length=248, Percent_Identity=29.8387096774194, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788748, Length=247, Percent_Identity=27.5303643724696, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI145693105, Length=190, Percent_Identity=23.6842105263158, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786401, Length=204, Percent_Identity=30.3921568627451, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790399, Length=241, Percent_Identity=24.896265560166, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788626, Length=140, Percent_Identity=32.1428571428571, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787589, Length=252, Percent_Identity=28.1746031746032, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI48994962, Length=113, Percent_Identity=37.1681415929204, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31624; Mature: 31624

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLATLEIFRTVVEAGGVTRAAERLHRVQSNVTTRIRQLEESLGVELFVRDGKRMVLTPA
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEECCC
GQTLLDYAGRMLRLADEARHAVLPSRPHGRLRIASMESTAASRLPAMLAAYHQAWSEVQL
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHEE
ELVTLPTRQAMEALERFEADCAFVAEPLGNPVLASRPAFEEELVVIARAGHRPIHSAADL
EEEECCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCHHHEEEEEECCCCCCCCCCCC
ETGTLLGFEPGCNYRARLEAWHAAGGGPAPRVLELNSYHAIVACAAAGIGAAIVPRSVLG
CCCEEEECCCCCCCHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHH
LYRDLPAISLHSLGEAGCVRTLLAWHPAMTSAALDALVQALPTPAAPAGNPALPAAA
HHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDLATLEIFRTVVEAGGVTRAAERLHRVQSNVTTRIRQLEESLGVELFVRDGKRMVLTPA
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEECCC
GQTLLDYAGRMLRLADEARHAVLPSRPHGRLRIASMESTAASRLPAMLAAYHQAWSEVQL
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHEE
ELVTLPTRQAMEALERFEADCAFVAEPLGNPVLASRPAFEEELVVIARAGHRPIHSAADL
EEEECCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCHHHEEEEEECCCCCCCCCCCC
ETGTLLGFEPGCNYRARLEAWHAAGGGPAPRVLELNSYHAIVACAAAGIGAAIVPRSVLG
CCCEEEECCCCCCCHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHH
LYRDLPAISLHSLGEAGCVRTLLAWHPAMTSAALDALVQALPTPAAPAGNPALPAAA
HHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]