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The map label for this gene is alaS [H]

Identifier: 73540553

GI number: 73540553

Start: 920856

End: 923480

Strand: Direct

Name: alaS [H]

Synonym: Reut_A0850

Alternate gene names: 73540553

Gene position: 920856-923480 (Clockwise)

Preceding gene: 73540549

Following gene: 73540554

Centisome position: 24.19

GC content: 64.88

Gene sequence:

>2625_bases
ATGAAAGTCTCAGACATTCGCAGCAAGTTCCTGCAGTTCTTCGAATCGAAGGGCCATACCGTGGTCCGCTCGTCCAGCCT
GGTGCCGGCGAATGACCCGACGCTGCTGTTCACCAACTCCGGCATGGTGCAGTTCAAGGACGTGTTCCTGGGCACCGACA
AGCGCCCGTACAGCCGCGCGACTTCGTCGCAGCGTTCGGTGCGCGCCGGCGGCAAGCACAATGACCTGGAGAACGTGGGC
TACACCGCCCGCCACCATACGTTCTTCGAGATGCTGGGCAACTTCTCGTTCGGCGACTACTTCAAGCGTGAAGCGATCCA
GTACGCGTGGGAACTGCTGACCAAGGTCTACCAGCTCCCGGCCGAGAAGCTGTGGGTGACGGTCTATGCGGAAGACGACG
AAGCCTATGACATCTGGGCCAAGGAAGTCGGCGTGCCGACCGAGCGCATCGTGCGCATCGGCGACAACAAGGGTTCGCGC
TACGCATCGGACAACTTCTGGCAGATGGCCGACACCGGCCCGTGCGGCCCGTGCTCGGAAATCTTCTTCGACCACGGCCC
GGACGTGTGGGGCGGCCCCCCGGGATCGCCCGAGGAAGACGGCGACCGCTACATCGAGATCTGGAACCTCGTGTTCATGC
AGTTCAACCGCGACGAGCAGGGCAACATGACGCCGCTGCCCAAGCCATGCGTGGACACCGGCATGGGCCTGGAGCGCATT
GCCGCGGTGCTGCAGCACGTGCACAGCAACTACGAGATCGACCTGTTCCAGGCGCTGATCAAGGCCGCCGCGCGTGAAAC
GCATATCGACAACCTGAACCAGAACTCGCTGAAGGTCATCGCCGACCATATCCGCGCGTGCTCGTTCCTGATCGTCGACG
GCGTGATCCCCGGCAACGAAGGCCGGGGCTACGTGCTGCGCCGTATCGTGCGCCGCGCGATCCGCCATGGCTACAAGCTG
GGCCAGAAGACCCCGTTCTTCCACAAGCTCGTGGCTGACCTCGTGGCGCAGATGGGTGAAGCCTATCCGGAACTGGCCGA
CGCCCAGGGCCGTGTGACCGAAGTGCTGAAGGCCGAGGAAGAGCGTTTCTTCGAGACCATCGAGAACGGCATGTCGATCC
TCGACGCTGCGCTGGCCGACCTGAAGCTCAAGGGCGGCAAGACGCTCGACGGCGAACTGGCGTTCAAGCTGCACGACACC
TTCGGCTTCCCGCTGGACCTGACGCAGGACGTCTGCCGCGAGCAGGAAATCGCCGTGGACGAGGCCGCGTTCGACGCCGC
CATGAACCGCCAGCGCGAGCAGGCACGTGCCGCCGGCAAGTTCAAGATGGCCGCTGGTCTGGAATACTCGGGCGACAAGA
CCGTGTTCCATGGCTACGAAAAGCTGGAGCTGCCGCAGGCAAAGATCATCGCACTGTACGTGGACGGCGCTGCTGTCGAC
GCCATGCAGCCGGGCCAGACCGGCGTGATCGTGCTCGACAACACCCCGTTCTACGCGGAATCCGGTGGCCAGGCTGGCGA
CCAGGGCGTGCTCAAGGCTGGCAATGCCGTGTTCGCCGTGGCTGACACCACCAAGATCCAGGCCGACGTGTTCGGTCACC
AGGGCACGCTGGAAGGCGGCGCGCTGAAGGTCGGCGACACCGTGCAGCCGCAGGTCGATGCACTGCGCCGCGCGCGCACC
GTGCGCAACCACTCGGCCACCCACCTGATGCACAAGGCGCTGCGCGAAGTGCTGGGCACCCACGTGCAACAGAAGGGCTC
GCTGGTCGATGCCGACAAGACGCGTTTCGACTTCTCGCACAACGCGCCGATGACCGACGAGCAGATCCGTCGCGTCGAAG
ACATCGTCAACGCCGAAATTCTGCGCAACGAAGACACGCACGCCGAGATCATGCCGTTCGACGACGCGGTCAAGAGCGGC
GCGATGGCACTGTTCGGCGAGAAGTACGCCGATGACGTGCGCGTGCTGTCGATCGGCACCTCGAAGGAACTGTGTGGCGG
CACCCACGTGCACCGCACCGGCGACATCGGCCTGTTCAAGATCGTGGTGGAAGGCGGTGTGGCCGCGGGCATCCGCCGCG
TGGAAGCGATCACCGGCGACAACGCGCTGCACTACTTGCAGAAGCTCGACGCCAAGCTCAACGAAGCCGCTGCCGCGCTG
AAGGCGCAGCCTTCGGAGCTGGTGCCGCGCATCGGCCAGGTGCAGGAGCAGGTACGCGCGCTCGAGAAGGAGTTGGAGCG
CCTGAAGAGCAAGCTCGCTTCGTCGCAAGGCGACGAGCTCGCCGAGCAGGCGGTCGACGTGAAGGGCCTCAAGGTGCTGG
CCGCCCAACTCGAAGGCGCAGATGTGAAGACGCTGCGCGAGACCATGGACAAGCTCAAGGACAAGCTGCAGAGCGCGGCG
ATCGTGCTGGCCGCAGTGGCCGACGGCAAGGTCAGCCTGATCGCCGGCGTGACCGCCGATGCGACCGGCAAGGTCAAGGC
TGGCGAGCTGGTCAACTTTGTCGCGCAGCAGGTTGGCGGCAAGGGCGGTGGCCGTCCGGATATGGCTCAGGCTGGCGGTA
CCGAGCCGGGCAAGCTGCCGCAGGCGCTGTCGGGTGTGGTGGAGTGGGTGTCGGCGAAGGTTTAA

Upstream 100 bases:

>100_bases
CATGCGTTTTTTGAGGCTGGCTTGCCCGTATAATCAACGGTTTGCAAATCCAACACTGATTCGTCGCGCCCTGCCTGCCG
CGGGAAGCGTCCGCCATCCC

Downstream 100 bases:

>100_bases
AGTTTTTCCGGCTAGGATAAAAGGGCGCCTCGGCGCCCTTTTTCTTTATCATCCAACGTCATGTATTGACCGTGTCCGGA
ACCACCATGAGAGCCACAGT

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 874; Mature: 874

Protein sequence:

>874_residues
MKVSDIRSKFLQFFESKGHTVVRSSSLVPANDPTLLFTNSGMVQFKDVFLGTDKRPYSRATSSQRSVRAGGKHNDLENVG
YTARHHTFFEMLGNFSFGDYFKREAIQYAWELLTKVYQLPAEKLWVTVYAEDDEAYDIWAKEVGVPTERIVRIGDNKGSR
YASDNFWQMADTGPCGPCSEIFFDHGPDVWGGPPGSPEEDGDRYIEIWNLVFMQFNRDEQGNMTPLPKPCVDTGMGLERI
AAVLQHVHSNYEIDLFQALIKAAARETHIDNLNQNSLKVIADHIRACSFLIVDGVIPGNEGRGYVLRRIVRRAIRHGYKL
GQKTPFFHKLVADLVAQMGEAYPELADAQGRVTEVLKAEEERFFETIENGMSILDAALADLKLKGGKTLDGELAFKLHDT
FGFPLDLTQDVCREQEIAVDEAAFDAAMNRQREQARAAGKFKMAAGLEYSGDKTVFHGYEKLELPQAKIIALYVDGAAVD
AMQPGQTGVIVLDNTPFYAESGGQAGDQGVLKAGNAVFAVADTTKIQADVFGHQGTLEGGALKVGDTVQPQVDALRRART
VRNHSATHLMHKALREVLGTHVQQKGSLVDADKTRFDFSHNAPMTDEQIRRVEDIVNAEILRNEDTHAEIMPFDDAVKSG
AMALFGEKYADDVRVLSIGTSKELCGGTHVHRTGDIGLFKIVVEGGVAAGIRRVEAITGDNALHYLQKLDAKLNEAAAAL
KAQPSELVPRIGQVQEQVRALEKELERLKSKLASSQGDELAEQAVDVKGLKVLAAQLEGADVKTLRETMDKLKDKLQSAA
IVLAAVADGKVSLIAGVTADATGKVKAGELVNFVAQQVGGKGGGRPDMAQAGGTEPGKLPQALSGVVEWVSAKV

Sequences:

>Translated_874_residues
MKVSDIRSKFLQFFESKGHTVVRSSSLVPANDPTLLFTNSGMVQFKDVFLGTDKRPYSRATSSQRSVRAGGKHNDLENVG
YTARHHTFFEMLGNFSFGDYFKREAIQYAWELLTKVYQLPAEKLWVTVYAEDDEAYDIWAKEVGVPTERIVRIGDNKGSR
YASDNFWQMADTGPCGPCSEIFFDHGPDVWGGPPGSPEEDGDRYIEIWNLVFMQFNRDEQGNMTPLPKPCVDTGMGLERI
AAVLQHVHSNYEIDLFQALIKAAARETHIDNLNQNSLKVIADHIRACSFLIVDGVIPGNEGRGYVLRRIVRRAIRHGYKL
GQKTPFFHKLVADLVAQMGEAYPELADAQGRVTEVLKAEEERFFETIENGMSILDAALADLKLKGGKTLDGELAFKLHDT
FGFPLDLTQDVCREQEIAVDEAAFDAAMNRQREQARAAGKFKMAAGLEYSGDKTVFHGYEKLELPQAKIIALYVDGAAVD
AMQPGQTGVIVLDNTPFYAESGGQAGDQGVLKAGNAVFAVADTTKIQADVFGHQGTLEGGALKVGDTVQPQVDALRRART
VRNHSATHLMHKALREVLGTHVQQKGSLVDADKTRFDFSHNAPMTDEQIRRVEDIVNAEILRNEDTHAEIMPFDDAVKSG
AMALFGEKYADDVRVLSIGTSKELCGGTHVHRTGDIGLFKIVVEGGVAAGIRRVEAITGDNALHYLQKLDAKLNEAAAAL
KAQPSELVPRIGQVQEQVRALEKELERLKSKLASSQGDELAEQAVDVKGLKVLAAQLEGADVKTLRETMDKLKDKLQSAA
IVLAAVADGKVSLIAGVTADATGKVKAGELVNFVAQQVGGKGGGRPDMAQAGGTEPGKLPQALSGVVEWVSAKV
>Mature_874_residues
MKVSDIRSKFLQFFESKGHTVVRSSSLVPANDPTLLFTNSGMVQFKDVFLGTDKRPYSRATSSQRSVRAGGKHNDLENVG
YTARHHTFFEMLGNFSFGDYFKREAIQYAWELLTKVYQLPAEKLWVTVYAEDDEAYDIWAKEVGVPTERIVRIGDNKGSR
YASDNFWQMADTGPCGPCSEIFFDHGPDVWGGPPGSPEEDGDRYIEIWNLVFMQFNRDEQGNMTPLPKPCVDTGMGLERI
AAVLQHVHSNYEIDLFQALIKAAARETHIDNLNQNSLKVIADHIRACSFLIVDGVIPGNEGRGYVLRRIVRRAIRHGYKL
GQKTPFFHKLVADLVAQMGEAYPELADAQGRVTEVLKAEEERFFETIENGMSILDAALADLKLKGGKTLDGELAFKLHDT
FGFPLDLTQDVCREQEIAVDEAAFDAAMNRQREQARAAGKFKMAAGLEYSGDKTVFHGYEKLELPQAKIIALYVDGAAVD
AMQPGQTGVIVLDNTPFYAESGGQAGDQGVLKAGNAVFAVADTTKIQADVFGHQGTLEGGALKVGDTVQPQVDALRRART
VRNHSATHLMHKALREVLGTHVQQKGSLVDADKTRFDFSHNAPMTDEQIRRVEDIVNAEILRNEDTHAEIMPFDDAVKSG
AMALFGEKYADDVRVLSIGTSKELCGGTHVHRTGDIGLFKIVVEGGVAAGIRRVEAITGDNALHYLQKLDAKLNEAAAAL
KAQPSELVPRIGQVQEQVRALEKELERLKSKLASSQGDELAEQAVDVKGLKVLAAQLEGADVKTLRETMDKLKDKLQSAA
IVLAAVADGKVSLIAGVTADATGKVKAGELVNFVAQQVGGKGGGRPDMAQAGGTEPGKLPQALSGVVEWVSAKV

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=965, Percent_Identity=37.5129533678756, Blast_Score=517, Evalue=1e-146,
Organism=Homo sapiens, GI38569417, Length=762, Percent_Identity=36.745406824147, Blast_Score=448, Evalue=1e-126,
Organism=Escherichia coli, GI1789048, Length=880, Percent_Identity=62.9545454545455, Blast_Score=1103, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=977, Percent_Identity=36.7451381780962, Blast_Score=543, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI17536681, Length=743, Percent_Identity=34.4549125168237, Blast_Score=388, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324911, Length=839, Percent_Identity=39.8092967818832, Blast_Score=556, Evalue=1e-159,
Organism=Drosophila melanogaster, GI24582809, Length=979, Percent_Identity=35.7507660878447, Blast_Score=535, Evalue=1e-152,
Organism=Drosophila melanogaster, GI45552267, Length=979, Percent_Identity=35.7507660878447, Blast_Score=535, Evalue=1e-152,
Organism=Drosophila melanogaster, GI24658214, Length=860, Percent_Identity=30.5813953488372, Blast_Score=343, Evalue=3e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 95418; Mature: 95418

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00087 SOD_CU_ZN_1 ; PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSDIRSKFLQFFESKGHTVVRSSSLVPANDPTLLFTNSGMVQFKDVFLGTDKRPYSRA
CCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECCCCEEEEHEEECCCCCCCHHH
TSSQRSVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKREAIQYAWELLTKVYQLP
CCCHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
AEKLWVTVYAEDDEAYDIWAKEVGVPTERIVRIGDNKGSRYASDNFWQMADTGPCGPCSE
CCCEEEEEEECCCCCHHHHHHHHCCCHHHEEEECCCCCCCCCCCCCEEECCCCCCCCHHH
IFFDHGPDVWGGPPGSPEEDGDRYIEIWNLVFMQFNRDEQGNMTPLPKPCVDTGMGLERI
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCHHHH
AAVLQHVHSNYEIDLFQALIKAAARETHIDNLNQNSLKVIADHIRACSFLIVDGVIPGNE
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEECCCCCCC
GRGYVLRRIVRRAIRHGYKLGQKTPFFHKLVADLVAQMGEAYPELADAQGRVTEVLKAEE
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCHHHHCCCHHHHHHHHHHH
ERFFETIENGMSILDAALADLKLKGGKTLDGELAFKLHDTFGFPLDLTQDVCREQEIAVD
HHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHH
EAAFDAAMNRQREQARAAGKFKMAAGLEYSGDKTVFHGYEKLELPQAKIIALYVDGAAVD
HHHHHHHHHHHHHHHHHCCCEEHHCCCCCCCCCCEECCHHHHCCCCEEEEEEEECCCEEC
AMQPGQTGVIVLDNTPFYAESGGQAGDQGVLKAGNAVFAVADTTKIQADVFGHQGTLEGG
CCCCCCCCEEEECCCCCEECCCCCCCCCCHHCCCCEEEEEECCCEEEHHHCCCCCCCCCC
ALKVGDTVQPQVDALRRARTVRNHSATHLMHKALREVLGTHVQQKGSLVDADKTRFDFSH
CEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEECCCC
NAPMTDEQIRRVEDIVNAEILRNEDTHAEIMPFDDAVKSGAMALFGEKYADDVRVLSIGT
CCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHCCCHHHHHHHHCCCEEEEEECC
SKELCGGTHVHRTGDIGLFKIVVEGGVAAGIRRVEAITGDNALHYLQKLDAKLNEAAAAL
CHHHCCCCCEEECCCCEEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
KAQPSELVPRIGQVQEQVRALEKELERLKSKLASSQGDELAEQAVDVKGLKVLAAQLEGA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHCCC
DVKTLRETMDKLKDKLQSAAIVLAAVADGKVSLIAGVTADATGKVKAGELVNFVAQQVGG
HHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCC
KGGGRPDMAQAGGTEPGKLPQALSGVVEWVSAKV
CCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVSDIRSKFLQFFESKGHTVVRSSSLVPANDPTLLFTNSGMVQFKDVFLGTDKRPYSRA
CCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECCCCEEEEHEEECCCCCCCHHH
TSSQRSVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKREAIQYAWELLTKVYQLP
CCCHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
AEKLWVTVYAEDDEAYDIWAKEVGVPTERIVRIGDNKGSRYASDNFWQMADTGPCGPCSE
CCCEEEEEEECCCCCHHHHHHHHCCCHHHEEEECCCCCCCCCCCCCEEECCCCCCCCHHH
IFFDHGPDVWGGPPGSPEEDGDRYIEIWNLVFMQFNRDEQGNMTPLPKPCVDTGMGLERI
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCHHHH
AAVLQHVHSNYEIDLFQALIKAAARETHIDNLNQNSLKVIADHIRACSFLIVDGVIPGNE
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEECCCCCCC
GRGYVLRRIVRRAIRHGYKLGQKTPFFHKLVADLVAQMGEAYPELADAQGRVTEVLKAEE
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCHHHHCCCHHHHHHHHHHH
ERFFETIENGMSILDAALADLKLKGGKTLDGELAFKLHDTFGFPLDLTQDVCREQEIAVD
HHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHH
EAAFDAAMNRQREQARAAGKFKMAAGLEYSGDKTVFHGYEKLELPQAKIIALYVDGAAVD
HHHHHHHHHHHHHHHHHCCCEEHHCCCCCCCCCCEECCHHHHCCCCEEEEEEEECCCEEC
AMQPGQTGVIVLDNTPFYAESGGQAGDQGVLKAGNAVFAVADTTKIQADVFGHQGTLEGG
CCCCCCCCEEEECCCCCEECCCCCCCCCCHHCCCCEEEEEECCCEEEHHHCCCCCCCCCC
ALKVGDTVQPQVDALRRARTVRNHSATHLMHKALREVLGTHVQQKGSLVDADKTRFDFSH
CEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEECCCC
NAPMTDEQIRRVEDIVNAEILRNEDTHAEIMPFDDAVKSGAMALFGEKYADDVRVLSIGT
CCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHCCCHHHHHHHHCCCEEEEEECC
SKELCGGTHVHRTGDIGLFKIVVEGGVAAGIRRVEAITGDNALHYLQKLDAKLNEAAAAL
CHHHCCCCCEEECCCCEEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
KAQPSELVPRIGQVQEQVRALEKELERLKSKLASSQGDELAEQAVDVKGLKVLAAQLEGA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHCCC
DVKTLRETMDKLKDKLQSAAIVLAAVADGKVSLIAGVTADATGKVKAGELVNFVAQQVGG
HHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCC
KGGGRPDMAQAGGTEPGKLPQALSGVVEWVSAKV
CCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA