Click here to switch to the map view.

The map label for this gene is pdxH

Identifier: 73540518

GI number: 73540518

Start: 884598

End: 885236

Strand: Reverse

Name: pdxH

Synonym: Reut_A0815

Alternate gene names: 73540518

Gene position: 885236-884598 (Counterclockwise)

Preceding gene: 73540519

Following gene: 73540517

Centisome position: 23.26

GC content: 65.57

Gene sequence:

>639_bases
ATGACCCAACTCGCTGACCTCCGCCGTACCTATGTCCTGGGCTCCCTCAACGAATCGGACGTGGCAGGCGACCCCATCGC
CCAGTTCAAGCGCTGGTTCGACGAGGCCGTGACCGCCAAGCTGCCCGAGCCCAATGCGATGACGCTGGCTACCGTCGGCG
CCGACGGCCAGCCGTCGGCACGCATCGTGCTTCTCAAAGGCATGGACGAGAAAGGCTTCACTTTCTTTACCAACTACGAA
AGCCGCAAGGGACTGGACATGGCCGCCAACCCGCGCGCCGCGCTGCTGTTCCACTGGGTGCAACTCGAGCGCCAGGTGCG
TGTGGAAGGCCGCGTTGAAAAAGTTGCCGACGACGAGAGCGATGCTTACTACGCGTCGCGCCCGCTCGGCTCACGCCTGG
GCGCCTGGGCTTCGGAGCAGAGCAGGGAAGTGCCGGGCCGCGATGTGCTCGAGCAGCGCGAATCCGAATACCGTGCGAAG
TTCGGCGAGAACCCGCCACGGCCTGCGCACTGGGGCGGCTATCGGCTCGTGCCGACCGCGCTCGAATTCTGGCAAGGGCG
GCCATCGCGGCTGCATGACCGCATTGCGTACCGCGTCGAAGCCGACGGTAGCTGGAAGATCGTGCGGCTGTCGCCTTGA

Upstream 100 bases:

>100_bases
TCCACCCTCGCATTGATTTGCCGGCGCACAAAAGCGGCTTGCAACGCATTCGCTATACTCAAATCAGCCGGATTTCCGGC
AGATTCCAATCGATTGCGAC

Downstream 100 bases:

>100_bases
TCGCTGCGCAGCACAAGCCGGGGAATTGTCCTAAAGTGGGATAAGCGTCGCTTTGAGGACGTGACGAGACACCGCGGGCG
AAAATCCCCGCGGGTCGCTG

Product: pyridoxamine 5'-phosphate oxidase

Products: NA

Alternate protein names: PNP/PMP oxidase; PNPOx; Pyridoxal 5'-phosphate synthase

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MTQLADLRRTYVLGSLNESDVAGDPIAQFKRWFDEAVTAKLPEPNAMTLATVGADGQPSARIVLLKGMDEKGFTFFTNYE
SRKGLDMAANPRAALLFHWVQLERQVRVEGRVEKVADDESDAYYASRPLGSRLGAWASEQSREVPGRDVLEQRESEYRAK
FGENPPRPAHWGGYRLVPTALEFWQGRPSRLHDRIAYRVEADGSWKIVRLSP

Sequences:

>Translated_212_residues
MTQLADLRRTYVLGSLNESDVAGDPIAQFKRWFDEAVTAKLPEPNAMTLATVGADGQPSARIVLLKGMDEKGFTFFTNYE
SRKGLDMAANPRAALLFHWVQLERQVRVEGRVEKVADDESDAYYASRPLGSRLGAWASEQSREVPGRDVLEQRESEYRAK
FGENPPRPAHWGGYRLVPTALEFWQGRPSRLHDRIAYRVEADGSWKIVRLSP
>Mature_211_residues
TQLADLRRTYVLGSLNESDVAGDPIAQFKRWFDEAVTAKLPEPNAMTLATVGADGQPSARIVLLKGMDEKGFTFFTNYES
RKGLDMAANPRAALLFHWVQLERQVRVEGRVEKVADDESDAYYASRPLGSRLGAWASEQSREVPGRDVLEQRESEYRAKF
GENPPRPAHWGGYRLVPTALEFWQGRPSRLHDRIAYRVEADGSWKIVRLSP

Specific function: Catalyzes the oxidation of either pyridoxine 5'- phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP)

COG id: COG0259

COG function: function code H; Pyridoxamine-phosphate oxidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyridoxamine 5'-phosphate oxidase family

Homologues:

Organism=Homo sapiens, GI8922498, Length=177, Percent_Identity=49.7175141242938, Blast_Score=183, Evalue=1e-46,
Organism=Escherichia coli, GI1787926, Length=213, Percent_Identity=47.4178403755869, Blast_Score=197, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI17553712, Length=195, Percent_Identity=45.1282051282051, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6319509, Length=210, Percent_Identity=46.6666666666667, Blast_Score=181, Evalue=1e-46,
Organism=Drosophila melanogaster, GI45551845, Length=199, Percent_Identity=42.713567839196, Blast_Score=159, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24644901, Length=199, Percent_Identity=42.713567839196, Blast_Score=159, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24644903, Length=182, Percent_Identity=30.2197802197802, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXH_CUPPJ (Q474I9)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_295038.1
- ProteinModelPortal:   Q474I9
- SMR:   Q474I9
- GeneID:   3610809
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A0815
- NMPDR:   fig|264198.3.peg.1328
- HOGENOM:   HBG327559
- OMA:   FTFFTNY
- ProtClustDB:   PRK05679
- BioCyc:   REUT264198:REUT_A0815-MONOMER
- HAMAP:   MF_01629
- InterPro:   IPR000659
- InterPro:   IPR019740
- InterPro:   IPR019576
- InterPro:   IPR011576
- InterPro:   IPR012349
- InterPro:   IPR009002
- Gene3D:   G3DSA:2.30.110.10
- PANTHER:   PTHR10851
- PIRSF:   PIRSF000190
- TIGRFAMs:   TIGR00558

Pfam domain/function: PF10590 PNPOx_C; PF01243 Pyridox_oxidase; SSF50475 FMN_binding

EC number: =1.4.3.5

Molecular weight: Translated: 23980; Mature: 23849

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS01064 PYRIDOX_OXIDASE

Important sites: BINDING 61-61 BINDING 64-64 BINDING 66-66 BINDING 83-83 BINDING 123-123 BINDING 127-127 BINDING 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLADLRRTYVLGSLNESDVAGDPIAQFKRWFDEAVTAKLPEPNAMTLATVGADGQPSA
CCCHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCE
RIVLLKGMDEKGFTFFTNYESRKGLDMAANPRAALLFHWVQLERQVRVEGRVEKVADDES
EEEEEECCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCC
DAYYASRPLGSRLGAWASEQSREVPGRDVLEQRESEYRAKFGENPPRPAHWGGYRLVPTA
CCEEECCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHH
LEFWQGRPSRLHDRIAYRVEADGSWKIVRLSP
HHHHCCCHHHHHHHEEEEEECCCCEEEEEECC
>Mature Secondary Structure 
TQLADLRRTYVLGSLNESDVAGDPIAQFKRWFDEAVTAKLPEPNAMTLATVGADGQPSA
CCHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCE
RIVLLKGMDEKGFTFFTNYESRKGLDMAANPRAALLFHWVQLERQVRVEGRVEKVADDES
EEEEEECCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCC
DAYYASRPLGSRLGAWASEQSREVPGRDVLEQRESEYRAKFGENPPRPAHWGGYRLVPTA
CCEEECCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHH
LEFWQGRPSRLHDRIAYRVEADGSWKIVRLSP
HHHHCCCHHHHHHHEEEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA