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The map label for this gene is pheS

Identifier: 71908286

GI number: 71908286

Start: 2870386

End: 2871432

Strand: Reverse

Name: pheS

Synonym: Daro_2673

Alternate gene names: 71908286

Gene position: 2871432-2870386 (Counterclockwise)

Preceding gene: 71908287

Following gene: 71908285

Centisome position: 63.79

GC content: 57.21

Gene sequence:

>1047_bases
ATGGAAAATCTCGATCAGATCGTTAGCGAAGCACAAGTTGCATTTGCTGCCATTTCCGACCCGGATGCGCTGGAACAGGT
CAAGGCCCGTTTCCTCGGCAAGAGCGGTCAGATTACCGAACTCCTTAAGGGCTTGGGCAAGCTGCCGCCCGAGGAGAAGA
AAACCGCCGGTGCAGCGATCAACGTCGCCAAGACTGCTGTTGAAACCGCGCTTAATGAGCGGCGCGAGGCGATCCGCAAG
GCTGCGCTGGATGCCCGTTTGGCTGAAGAGGCGTTGGATGTGACGCTGCCCGGTCGCGCTGAAGCGCGCGGTGGCCTTCA
CCCGGTGACACGTACGCTGGAGCGTATCGAATCTTTGTTCGCCTCGATCGGCTTTGAGGTCGCTGACGGCCCGGAAATCG
AAGAGGATTTCTACAATTTTACGGCCATGAACACGCCCGAGGATCATCCTGCACGTTCGATGCACGATACCTTTTACCTG
CAGAACTCGGACGGTACTTTGGCCGACAAGGTGCTGCTGCGCACTCATACCAGCCCGATCCAGGCCCGATACATGCAAGC
GCATGTAAAGCGCTACGGCCAGCTGGGAAAAATGCCTGAAATCCGCATCATCGCGCCGGGGCGCGTCTATCGCGTCGATT
CCGATGCGACCCATTCGCCAATGTTTCATCAGGTCGAAGGCCTGTGGGTTGGTGAAGGTGTTTCCTTCGCCGACCTGAAG
GGCGTCATTGCCAATTTTCTGAAAAGCTTCTTTGAGACGGACGACCTGACGGTGCGTTTCCGTCCATCCTTCTTCCCGTT
TACCGAGCCATCGGCTGAAATTGACGTCGCCTTCATGAGCGGTGCGCTGAAGGGCCGATGGCTGGAAATCGCCGGTTGCG
GCATGGTCCATCCTAACGTGCTGCGCATTGCCGGCATTGATCCGGAAAAGTACACCGGCTTTGCCTTTGGCTTCGGTCAG
GATCGCCTGACCATGCTGCGCTACGGCATCAATGATCTTCGCCTGTTCTTTGAAGGCGACCTGCGTTTCCTGCGTCAGTT
CAACTGA

Upstream 100 bases:

>100_bases
GGCACAGCTCGGCGCCTGAGCGAAAGCTTGATGAAAAAAAGGAGGCGCGAGCCTCCTTTTTTGTTTGTGGTTTTGTCGCA
GTTTGCAGGTATTGAAGTCC

Downstream 100 bases:

>100_bases
CCTACAATTCGAGTCACGCCAATGAAATTCTCCGAATCCTGGCTGCGTACCCTCGTCGATCCCAAATTGTCGAGCGAGGA
ACTCTCCCATCTGTTGACTA

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MENLDQIVSEAQVAFAAISDPDALEQVKARFLGKSGQITELLKGLGKLPPEEKKTAGAAINVAKTAVETALNERREAIRK
AALDARLAEEALDVTLPGRAEARGGLHPVTRTLERIESLFASIGFEVADGPEIEEDFYNFTAMNTPEDHPARSMHDTFYL
QNSDGTLADKVLLRTHTSPIQARYMQAHVKRYGQLGKMPEIRIIAPGRVYRVDSDATHSPMFHQVEGLWVGEGVSFADLK
GVIANFLKSFFETDDLTVRFRPSFFPFTEPSAEIDVAFMSGALKGRWLEIAGCGMVHPNVLRIAGIDPEKYTGFAFGFGQ
DRLTMLRYGINDLRLFFEGDLRFLRQFN

Sequences:

>Translated_348_residues
MENLDQIVSEAQVAFAAISDPDALEQVKARFLGKSGQITELLKGLGKLPPEEKKTAGAAINVAKTAVETALNERREAIRK
AALDARLAEEALDVTLPGRAEARGGLHPVTRTLERIESLFASIGFEVADGPEIEEDFYNFTAMNTPEDHPARSMHDTFYL
QNSDGTLADKVLLRTHTSPIQARYMQAHVKRYGQLGKMPEIRIIAPGRVYRVDSDATHSPMFHQVEGLWVGEGVSFADLK
GVIANFLKSFFETDDLTVRFRPSFFPFTEPSAEIDVAFMSGALKGRWLEIAGCGMVHPNVLRIAGIDPEKYTGFAFGFGQ
DRLTMLRYGINDLRLFFEGDLRFLRQFN
>Mature_348_residues
MENLDQIVSEAQVAFAAISDPDALEQVKARFLGKSGQITELLKGLGKLPPEEKKTAGAAINVAKTAVETALNERREAIRK
AALDARLAEEALDVTLPGRAEARGGLHPVTRTLERIESLFASIGFEVADGPEIEEDFYNFTAMNTPEDHPARSMHDTFYL
QNSDGTLADKVLLRTHTSPIQARYMQAHVKRYGQLGKMPEIRIIAPGRVYRVDSDATHSPMFHQVEGLWVGEGVSFADLK
GVIANFLKSFFETDDLTVRFRPSFFPFTEPSAEIDVAFMSGALKGRWLEIAGCGMVHPNVLRIAGIDPEKYTGFAFGFGQ
DRLTMLRYGINDLRLFFEGDLRFLRQFN

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=266, Percent_Identity=32.3308270676692, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI5729820, Length=299, Percent_Identity=29.4314381270903, Blast_Score=112, Evalue=4e-25,
Organism=Escherichia coli, GI1788007, Length=347, Percent_Identity=56.1959654178674, Blast_Score=393, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI32563657, Length=274, Percent_Identity=33.2116788321168, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17508957, Length=274, Percent_Identity=33.2116788321168, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI32566635, Length=258, Percent_Identity=31.3953488372093, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321087, Length=377, Percent_Identity=28.1167108753316, Blast_Score=136, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6325304, Length=94, Percent_Identity=44.6808510638298, Blast_Score=90, Evalue=4e-19,
Organism=Drosophila melanogaster, GI18858079, Length=271, Percent_Identity=31.3653136531365, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI17137424, Length=259, Percent_Identity=27.027027027027, Blast_Score=95, Evalue=7e-20,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_DECAR (Q47CM8)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_285873.1
- ProteinModelPortal:   Q47CM8
- SMR:   Q47CM8
- STRING:   Q47CM8
- GeneID:   3567014
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_2673
- NMPDR:   fig|159087.4.peg.3449
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- BioCyc:   DARO159087:DARO_2673-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 38716; Mature: 38716

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENLDQIVSEAQVAFAAISDPDALEQVKARFLGKSGQITELLKGLGKLPPEEKKTAGAAI
CCHHHHHHHHHHHHHEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHCCHHH
NVAKTAVETALNERREAIRKAALDARLAEEALDVTLPGRAEARGGLHPVTRTLERIESLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCHHHHHHHHHHHHH
ASIGFEVADGPEIEEDFYNFTAMNTPEDHPARSMHDTFYLQNSDGTLADKVLLRTHTSPI
HHCCCCCCCCCCHHHHHHCCEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCHH
QARYMQAHVKRYGQLGKMPEIRIIAPGRVYRVDSDATHSPMFHQVEGLWVGEGVSFADLK
HHHHHHHHHHHHHHCCCCCCEEEECCCEEEEECCCCCCCCHHHHHCCEEEECCCCHHHHH
GVIANFLKSFFETDDLTVRFRPSFFPFTEPSAEIDVAFMSGALKGRWLEIAGCGMVHPNV
HHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCEEEHHHCCCCCCCEEEEECCCCCCCCE
LRIAGIDPEKYTGFAFGFGQDRLTMLRYGINDLRLFFEGDLRFLRQFN
EEEECCCCCCCCCEEEECCCHHHHHHHHCHHHEEEEEHHHHHHHHCCC
>Mature Secondary Structure
MENLDQIVSEAQVAFAAISDPDALEQVKARFLGKSGQITELLKGLGKLPPEEKKTAGAAI
CCHHHHHHHHHHHHHEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHCCHHH
NVAKTAVETALNERREAIRKAALDARLAEEALDVTLPGRAEARGGLHPVTRTLERIESLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCHHHHHHHHHHHHH
ASIGFEVADGPEIEEDFYNFTAMNTPEDHPARSMHDTFYLQNSDGTLADKVLLRTHTSPI
HHCCCCCCCCCCHHHHHHCCEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCHH
QARYMQAHVKRYGQLGKMPEIRIIAPGRVYRVDSDATHSPMFHQVEGLWVGEGVSFADLK
HHHHHHHHHHHHHHCCCCCCEEEECCCEEEEECCCCCCCCHHHHHCCEEEECCCCHHHHH
GVIANFLKSFFETDDLTVRFRPSFFPFTEPSAEIDVAFMSGALKGRWLEIAGCGMVHPNV
HHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCEEEHHHCCCCCCCEEEEECCCCCCCCE
LRIAGIDPEKYTGFAFGFGQDRLTMLRYGINDLRLFFEGDLRFLRQFN
EEEECCCCCCCCCEEEECCCHHHHHHHHCHHHEEEEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA