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The map label for this gene is nirT [H]

Identifier: 71908193

GI number: 71908193

Start: 2776744

End: 2777319

Strand: Reverse

Name: nirT [H]

Synonym: Daro_2579

Alternate gene names: 71908193

Gene position: 2777319-2776744 (Counterclockwise)

Preceding gene: 71908194

Following gene: 71908192

Centisome position: 61.7

GC content: 47.4

Gene sequence:

>576_bases
ATGAAAAACATTCTCAGATCGCTACGCCAACCAAGCACTAAATATTCACTTTTGGGCTTGCTCTCTATAGGCTTTATCGC
AGGCATCTTCTTTTGGGGGGGCTTTAATACCGTAATGGAAGCCACCAACACACTTGAATTCTGTATTTCATGTCATGAAA
TGCGTGACAACGTATATCAGGAATACAAGGAAACCATTCACTTTACCAATCGCACTGGCGTTCGCGCCGTTTGCTCGGAC
TGCCATGTACCCAAGGACTGGGGCCACAAGATGATGCGCAAGATCCAAGCGAGTGGCGAAGTCTACGGCAAACTCATGGG
AACCATTGATACCAAGGAAAAGTTTGAAGCCAAGCGCCTCGAACTAGCCGAACACGAATGGGCACGAATGAAAGCCAGCG
ATTCGCGCGAATGCCGCAACTGTCACAGTTTTGAAGGCATGAACACGGAAAAGCAAAAATTACGCGGTGCCAAAATGCAC
AAAATCGCTCAGGACGATAAAAAGACCTGTATCGATTGCCACAAAGGTATCGCCCACCGCAAACCGAAAGGTATGAAAGA
GGAAGATGACGAGTGA

Upstream 100 bases:

>100_bases
GTCGCCATATGCCTCGGTATTTGCGCATTTGAGAATCTGAAACTCACCTTGCTCGTTTCAGATCGCCGAGTAACCGAATC
GCAATTAAGCATGCTAATCA

Downstream 100 bases:

>100_bases
TCGTCGCCTATCGGTGACACCAATCATCGACAACCGTCTGGAGAAATTAAATGAAAAAAAGTGCCATGTCAGTCGCCATT
CTTGGTGCCGTATTTTCCCT

Product: NapC/NirT cytochrome c, N-terminal

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MKNILRSLRQPSTKYSLLGLLSIGFIAGIFFWGGFNTVMEATNTLEFCISCHEMRDNVYQEYKETIHFTNRTGVRAVCSD
CHVPKDWGHKMMRKIQASGEVYGKLMGTIDTKEKFEAKRLELAEHEWARMKASDSRECRNCHSFEGMNTEKQKLRGAKMH
KIAQDDKKTCIDCHKGIAHRKPKGMKEEDDE

Sequences:

>Translated_191_residues
MKNILRSLRQPSTKYSLLGLLSIGFIAGIFFWGGFNTVMEATNTLEFCISCHEMRDNVYQEYKETIHFTNRTGVRAVCSD
CHVPKDWGHKMMRKIQASGEVYGKLMGTIDTKEKFEAKRLELAEHEWARMKASDSRECRNCHSFEGMNTEKQKLRGAKMH
KIAQDDKKTCIDCHKGIAHRKPKGMKEEDDE
>Mature_191_residues
MKNILRSLRQPSTKYSLLGLLSIGFIAGIFFWGGFNTVMEATNTLEFCISCHEMRDNVYQEYKETIHFTNRTGVRAVCSD
CHVPKDWGHKMMRKIQASGEVYGKLMGTIDTKEKFEAKRLELAEHEWARMKASDSRECRNCHSFEGMNTEKQKLRGAKMH
KIAQDDKKTCIDCHKGIAHRKPKGMKEEDDE

Specific function: Mediates Electron Flow From Quinones To The Napab Complex. [C]

COG id: COG3005

COG function: function code C; Nitrate/TMAO reductases, membrane-bound tetraheme cytochrome c subunit

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788530, Length=182, Percent_Identity=59.3406593406593, Blast_Score=233, Evalue=6e-63,
Organism=Escherichia coli, GI1787230, Length=195, Percent_Identity=46.1538461538462, Blast_Score=192, Evalue=1e-50,
Organism=Escherichia coli, GI1788181, Length=147, Percent_Identity=46.9387755102041, Blast_Score=151, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011031
- InterPro:   IPR005126
- InterPro:   IPR011885 [H]

Pfam domain/function: PF03264 Cytochrom_NNT [H]

EC number: NA

Molecular weight: Translated: 22069; Mature: 22069

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
9.4 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
9.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNILRSLRQPSTKYSLLGLLSIGFIAGIFFWGGFNTVMEATNTLEFCISCHEMRDNVYQ
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EYKETIHFTNRTGVRAVCSDCHVPKDWGHKMMRKIQASGEVYGKLMGTIDTKEKFEAKRL
HHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
ELAEHEWARMKASDSRECRNCHSFEGMNTEKQKLRGAKMHKIAQDDKKTCIDCHKGIAHR
HHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KPKGMKEEDDE
CCCCCCCCCCC
>Mature Secondary Structure
MKNILRSLRQPSTKYSLLGLLSIGFIAGIFFWGGFNTVMEATNTLEFCISCHEMRDNVYQ
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EYKETIHFTNRTGVRAVCSDCHVPKDWGHKMMRKIQASGEVYGKLMGTIDTKEKFEAKRL
HHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
ELAEHEWARMKASDSRECRNCHSFEGMNTEKQKLRGAKMHKIAQDDKKTCIDCHKGIAHR
HHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KPKGMKEEDDE
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2001732 [H]