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The map label for this gene is moaA [H]

Identifier: 71908191

GI number: 71908191

Start: 2774444

End: 2775415

Strand: Reverse

Name: moaA [H]

Synonym: Daro_2577

Alternate gene names: 71908191

Gene position: 2775415-2774444 (Counterclockwise)

Preceding gene: 71908192

Following gene: 71908184

Centisome position: 61.66

GC content: 49.69

Gene sequence:

>972_bases
ATGTTAATTGATGGTTGTGGTCGTCGGATAGATTATCTCCGATTGTCGGTGACTGATCGTTGCGACTTGCGATGCGCTTA
CTGCATGCCGCCGGAGTTTAACGATTATGAAATTCCCAATCATTGGTTAATGTTCAGTGAGATCGTGAGGGTTTGCAGGA
TATTCATTGATCTTGGGGTTTCCAGAGTGCGTCTCACTGGCGGCGAACCCTTGGTAAGATCCAGAATCGGCGATCTAGTC
CAAGAAATCGGACAACTTCCCGGACTGAACGATCTTTCCCTCAGCACCAATGGCACACGATTGAAGCAATTTGCACCTAA
ACTTCTTGCTTCCGGTGTTACGCGACTAAATGTTAGCCTTGATACGTTGTCGCCAAGTCGATTTCTGACATTGACTCGGC
GTGACGCTCTGCACGATGTCCTCGCAGGACTCGATGTGGCGAGAAAGTGTGGATTTCGCCTGATAAAGATCAATATGGTT
TGGTTGCCCGAATTCAACGGTAACGAACTAGACGCAATGATCGACTACTGCATGGAACGCAGTTTTGTGTTGCGCTTGAT
CGAGAACATGCCAATGGGGGATACTGCACGTCAACTTGGCACCTCATCCCTCCAGCCCCTAATCGGCCACCTTAGAGAGC
GCTTCCATCTGGTCGATCACATCGTTGCTGGCGGAGGTCCTGCTCGCTACCTTGCGACTCCGGATCGGTCCTTCTCTGTC
GGTTTTATTACCCCTATGTCCCAACACTTTTGCGAGGCATGCAACCGTGTCAGGCTTTCGGTAACCGGCACGTTACACTT
ATGTCTTGGTCAGAATGACCACCTCGAACTATTGCCAATGTTGCGTGGAGGATCGACCGATGAGGAAATCGCCGATCAAA
TTCGCGCAGCAGTTATGCAAAAGCCATTCAGCCACGAATTCCAAACAAATCCCAAGAAGATCGTTCGTATCATGGCATCG
ACTGGTGGATAA

Upstream 100 bases:

>100_bases
CAGCATTTCCGTTGGCAATAAGATGCAACATTGTCTTTTTGCATTTTTTTGGTCGTTGGCGCGTAAAAGTTCGCGCCTAT
TTTGGTGAAGTCATGGTTCG

Downstream 100 bases:

>100_bases
TTAGCGCTACGGCATCTATAAATGTGCTGCTTCATGCTTGGGAATGAGCAGCGGTTGAAAGCATGCCACATGCCATTTCC
ATGACAACTGCCGATGTGGC

Product: radical SAM:molybdenum cofactor synthesis C-terminal

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MLIDGCGRRIDYLRLSVTDRCDLRCAYCMPPEFNDYEIPNHWLMFSEIVRVCRIFIDLGVSRVRLTGGEPLVRSRIGDLV
QEIGQLPGLNDLSLSTNGTRLKQFAPKLLASGVTRLNVSLDTLSPSRFLTLTRRDALHDVLAGLDVARKCGFRLIKINMV
WLPEFNGNELDAMIDYCMERSFVLRLIENMPMGDTARQLGTSSLQPLIGHLRERFHLVDHIVAGGGPARYLATPDRSFSV
GFITPMSQHFCEACNRVRLSVTGTLHLCLGQNDHLELLPMLRGGSTDEEIADQIRAAVMQKPFSHEFQTNPKKIVRIMAS
TGG

Sequences:

>Translated_323_residues
MLIDGCGRRIDYLRLSVTDRCDLRCAYCMPPEFNDYEIPNHWLMFSEIVRVCRIFIDLGVSRVRLTGGEPLVRSRIGDLV
QEIGQLPGLNDLSLSTNGTRLKQFAPKLLASGVTRLNVSLDTLSPSRFLTLTRRDALHDVLAGLDVARKCGFRLIKINMV
WLPEFNGNELDAMIDYCMERSFVLRLIENMPMGDTARQLGTSSLQPLIGHLRERFHLVDHIVAGGGPARYLATPDRSFSV
GFITPMSQHFCEACNRVRLSVTGTLHLCLGQNDHLELLPMLRGGSTDEEIADQIRAAVMQKPFSHEFQTNPKKIVRIMAS
TGG
>Mature_323_residues
MLIDGCGRRIDYLRLSVTDRCDLRCAYCMPPEFNDYEIPNHWLMFSEIVRVCRIFIDLGVSRVRLTGGEPLVRSRIGDLV
QEIGQLPGLNDLSLSTNGTRLKQFAPKLLASGVTRLNVSLDTLSPSRFLTLTRRDALHDVLAGLDVARKCGFRLIKINMV
WLPEFNGNELDAMIDYCMERSFVLRLIENMPMGDTARQLGTSSLQPLIGHLRERFHLVDHIVAGGGPARYLATPDRSFSV
GFITPMSQHFCEACNRVRLSVTGTLHLCLGQNDHLELLPMLRGGSTDEEIADQIRAAVMQKPFSHEFQTNPKKIVRIMAS
TGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z [H]

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family [H]

Homologues:

Organism=Homo sapiens, GI116734668, Length=307, Percent_Identity=33.8762214983713, Blast_Score=193, Evalue=1e-49,
Organism=Homo sapiens, GI22027536, Length=307, Percent_Identity=33.8762214983713, Blast_Score=193, Evalue=2e-49,
Organism=Escherichia coli, GI1786999, Length=326, Percent_Identity=32.8220858895706, Blast_Score=194, Evalue=6e-51,
Organism=Caenorhabditis elegans, GI32566331, Length=309, Percent_Identity=35.2750809061489, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI32566329, Length=309, Percent_Identity=35.2750809061489, Blast_Score=191, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17561210, Length=309, Percent_Identity=34.6278317152104, Blast_Score=185, Evalue=3e-47,
Organism=Drosophila melanogaster, GI28574488, Length=309, Percent_Identity=38.1877022653722, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI28574490, Length=309, Percent_Identity=38.1877022653722, Blast_Score=200, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 36213; Mature: 36213

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS01305 MOAA_NIFB_PQQE ; PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIDGCGRRIDYLRLSVTDRCDLRCAYCMPPEFNDYEIPNHWLMFSEIVRVCRIFIDLGV
CCCCCCCCEEEEEEEEECCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
SRVRLTGGEPLVRSRIGDLVQEIGQLPGLNDLSLSTNGTRLKQFAPKLLASGVTRLNVSL
CEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHCCCCEEEEEE
DTLSPSRFLTLTRRDALHDVLAGLDVARKCGFRLIKINMVWLPEFNGNELDAMIDYCMER
ECCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCHHHHHHHHHHHH
SFVLRLIENMPMGDTARQLGTSSLQPLIGHLRERFHLVDHIVAGGGPARYLATPDRSFSV
HHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEE
GFITPMSQHFCEACNRVRLSVTGTLHLCLGQNDHLELLPMLRGGSTDEEIADQIRAAVMQ
EEECHHHHHHHHHHCCEEEEEEEEEEEEECCCCCEEEEEHHCCCCCHHHHHHHHHHHHHH
KPFSHEFQTNPKKIVRIMASTGG
CCCCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MLIDGCGRRIDYLRLSVTDRCDLRCAYCMPPEFNDYEIPNHWLMFSEIVRVCRIFIDLGV
CCCCCCCCEEEEEEEEECCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
SRVRLTGGEPLVRSRIGDLVQEIGQLPGLNDLSLSTNGTRLKQFAPKLLASGVTRLNVSL
CEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHCCCCEEEEEE
DTLSPSRFLTLTRRDALHDVLAGLDVARKCGFRLIKINMVWLPEFNGNELDAMIDYCMER
ECCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCHHHHHHHHHHHH
SFVLRLIENMPMGDTARQLGTSSLQPLIGHLRERFHLVDHIVAGGGPARYLATPDRSFSV
HHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEE
GFITPMSQHFCEACNRVRLSVTGTLHLCLGQNDHLELLPMLRGGSTDEEIADQIRAAVMQ
EEECHHHHHHHHHHCCEEEEEEEEEEEEECCCCCEEEEEHHCCCCCHHHHHHHHHHHHHH
KPFSHEFQTNPKKIVRIMASTGG
CCCCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA