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The map label for this gene is pyrG

Identifier: 71907985

GI number: 71907985

Start: 2550089

End: 2551729

Strand: Reverse

Name: pyrG

Synonym: Daro_2366

Alternate gene names: 71907985

Gene position: 2551729-2550089 (Counterclockwise)

Preceding gene: 71908000

Following gene: 71907984

Centisome position: 56.69

GC content: 57.53

Gene sequence:

>1641_bases
ATGACCAAATATGTTTTCGTTACGGGTGGTGTCGTGTCCTCTCTGGGCAAAGGCATCGCCGCCGCGTCGCTCGGGGCCAT
TCTGGAATCCCGCGGCATCAAAGTCACCCACCTCAAGCTTGACCCCTACATCAACGTCGACCCCGGTACGATGAGTCCTT
TCCAGCATGGAGAGGTTTTCGTCACCGATGACGGAGCCGAGACCGATCTGGATCTGGGGCATTACGAGCGCTTTACCAGC
GCCAAGATGGCCAAGCGCAACAACTTCACGACCGGCCAGATTTACGATTCGGTGCTCAAGAAAGAGCGTCGTGGCGAATA
TCTCGGCAAGACCGTGCAAGTCATCCCGCACATTACCGACGAGATCAAGGATCGCATCAAGGCCGGCGCCGAAGGTGCAG
ACGTGGCCATCGTTGAAGTCGGTGGCACGGTTGGCGACATCGAGTCGCTGCCCTTCCTCGAAGCCATTCGTCAAATGGGT
ATCATGGAGGGGCGTAACAACGTCTGCTTCATGCATCTGACGCTGTTGCCCTACATCCCGACTGCCGGTGAGCTGAAGAC
CAAGCCGACTCAGCATTCCGTCAAGGAACTGCGCGAAATCGGTATCCAGCCGGACATCCTGCTGTGTCGCGCCGATCGTT
CGATTCCAGTCGAAGAGCGCCGCAAGATCGCACTGTTCTGCAATGTGATGCCGGAAGCCGTCATCGAGTGTCTTGATGCC
GATTCGATCTACAAGATTCCGGGCATGCTGCACGAACAGATGCTCGACGAGATCGTCTGCCACAAGCTGAATATCCTGGC
CAAGGCAGCCGACCTGACGGTCTGGGAAAATCTGATCGTGGCGCTGGAGCACCCGCTGCACCAGGTCAAGATCGCTTTTG
TCGGCAAGTACGTTGATCTGACCGAGTCCTACAAGTCCTTGATTGAAGCCATCAAGCATGCCGGTATCCATACCCGCAGC
CAGGTCAACATCATTTATCTCGATTCCGAAGATATCGAGAAGAATGGCACCGGCGTCCTTGAAGACCTCGATGCCATCCT
CGTTCCCGGCGGTTTCGGTCGTCGCGGTACGGAAGGCAAGATTGCCGCGATCCGCTATGCCCGCGAGAACAAGGTGCCCT
ATCTCGGTATCTGTCTCGGCATGCAACTGGCCGTCGTTGAATTCGCTCGTGATGTGGCCGGTATGCCCGGTGCGCACTCC
ACCGAATTCGTGCAGGACACGCCATACCCGGTAATCGGCCTGATTACCGAGTGGCTGGACGCTTCCGGCAAGATCGAGAA
GCGTACCGAGGATTCCGATATCGGCGGCACCATGCGTCTTGGCGCTCAGGTCTGCAAGCTGGGTGAGGGCACGCTGGCTC
GTAGCATCTATGGCGCGCCGGAAATCACCGAACGCCATCGTCACCGCTACGAAGTGAACAACACGCTGCTCGCCAAGCTG
GAAGAAAAGGGCCTGATCGTCGCCGGTCGTGCCCCTGGTACCGATCTGTGCGAAATGGTCGAGTTGCCGGCCGATGTGCA
TCCGTGGTTCGTCGGTTGTCAGTTCCACCCGGAATTCACCTCCAATCCGCGTCAAGGTCATCCGCTCTTCTCTTCTTACG
TGAAGGCGGCGCTGGCCAATCAAAAGGCCAAAGGCAAATGA

Upstream 100 bases:

>100_bases
GCTACGGCACTAGTCACTTTGAATGAGGGCTTGTGTCGACGGGCTTGGGAAAATGTCAGCTTATCGGCTATCATTCTGCT
TTCCCGCAGCCTGTCTTTCC

Downstream 100 bases:

>100_bases
AGCTCTGCGGTTTCGAGGCTGGTCTTGACCAGCCTTTTTTCCTGATTGCCGGGCCATGTACTGCTGAATCCGAGCAGCTG
TGCCTCGATGTGGCTGGCCA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MTKYVFVTGGVVSSLGKGIAAASLGAILESRGIKVTHLKLDPYINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTS
AKMAKRNNFTTGQIYDSVLKKERRGEYLGKTVQVIPHITDEIKDRIKAGAEGADVAIVEVGGTVGDIESLPFLEAIRQMG
IMEGRNNVCFMHLTLLPYIPTAGELKTKPTQHSVKELREIGIQPDILLCRADRSIPVEERRKIALFCNVMPEAVIECLDA
DSIYKIPGMLHEQMLDEIVCHKLNILAKAADLTVWENLIVALEHPLHQVKIAFVGKYVDLTESYKSLIEAIKHAGIHTRS
QVNIIYLDSEDIEKNGTGVLEDLDAILVPGGFGRRGTEGKIAAIRYARENKVPYLGICLGMQLAVVEFARDVAGMPGAHS
TEFVQDTPYPVIGLITEWLDASGKIEKRTEDSDIGGTMRLGAQVCKLGEGTLARSIYGAPEITERHRHRYEVNNTLLAKL
EEKGLIVAGRAPGTDLCEMVELPADVHPWFVGCQFHPEFTSNPRQGHPLFSSYVKAALANQKAKGK

Sequences:

>Translated_546_residues
MTKYVFVTGGVVSSLGKGIAAASLGAILESRGIKVTHLKLDPYINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTS
AKMAKRNNFTTGQIYDSVLKKERRGEYLGKTVQVIPHITDEIKDRIKAGAEGADVAIVEVGGTVGDIESLPFLEAIRQMG
IMEGRNNVCFMHLTLLPYIPTAGELKTKPTQHSVKELREIGIQPDILLCRADRSIPVEERRKIALFCNVMPEAVIECLDA
DSIYKIPGMLHEQMLDEIVCHKLNILAKAADLTVWENLIVALEHPLHQVKIAFVGKYVDLTESYKSLIEAIKHAGIHTRS
QVNIIYLDSEDIEKNGTGVLEDLDAILVPGGFGRRGTEGKIAAIRYARENKVPYLGICLGMQLAVVEFARDVAGMPGAHS
TEFVQDTPYPVIGLITEWLDASGKIEKRTEDSDIGGTMRLGAQVCKLGEGTLARSIYGAPEITERHRHRYEVNNTLLAKL
EEKGLIVAGRAPGTDLCEMVELPADVHPWFVGCQFHPEFTSNPRQGHPLFSSYVKAALANQKAKGK
>Mature_545_residues
TKYVFVTGGVVSSLGKGIAAASLGAILESRGIKVTHLKLDPYINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTSA
KMAKRNNFTTGQIYDSVLKKERRGEYLGKTVQVIPHITDEIKDRIKAGAEGADVAIVEVGGTVGDIESLPFLEAIRQMGI
MEGRNNVCFMHLTLLPYIPTAGELKTKPTQHSVKELREIGIQPDILLCRADRSIPVEERRKIALFCNVMPEAVIECLDAD
SIYKIPGMLHEQMLDEIVCHKLNILAKAADLTVWENLIVALEHPLHQVKIAFVGKYVDLTESYKSLIEAIKHAGIHTRSQ
VNIIYLDSEDIEKNGTGVLEDLDAILVPGGFGRRGTEGKIAAIRYARENKVPYLGICLGMQLAVVEFARDVAGMPGAHST
EFVQDTPYPVIGLITEWLDASGKIEKRTEDSDIGGTMRLGAQVCKLGEGTLARSIYGAPEITERHRHRYEVNNTLLAKLE
EKGLIVAGRAPGTDLCEMVELPADVHPWFVGCQFHPEFTSNPRQGHPLFSSYVKAALANQKAKGK

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI28559085, Length=565, Percent_Identity=44.070796460177, Blast_Score=475, Evalue=1e-134,
Organism=Homo sapiens, GI28559083, Length=565, Percent_Identity=44.070796460177, Blast_Score=475, Evalue=1e-134,
Organism=Homo sapiens, GI221316689, Length=565, Percent_Identity=44.070796460177, Blast_Score=475, Evalue=1e-134,
Organism=Homo sapiens, GI148491070, Length=561, Percent_Identity=44.5632798573975, Blast_Score=465, Evalue=1e-131,
Organism=Escherichia coli, GI1789142, Length=545, Percent_Identity=64.2201834862385, Blast_Score=723, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25148299, Length=613, Percent_Identity=38.4991843393148, Blast_Score=409, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6322563, Length=572, Percent_Identity=42.3076923076923, Blast_Score=450, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6319432, Length=552, Percent_Identity=42.7536231884058, Blast_Score=443, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24664469, Length=560, Percent_Identity=42.6785714285714, Blast_Score=453, Evalue=1e-127,
Organism=Drosophila melanogaster, GI21357815, Length=506, Percent_Identity=40.9090909090909, Blast_Score=379, Evalue=1e-105,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_DECAR (Q47DH9)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_285572.1
- HSSP:   P0A7E5
- ProteinModelPortal:   Q47DH9
- SMR:   Q47DH9
- STRING:   Q47DH9
- GeneID:   3566048
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_2366
- NMPDR:   fig|159087.4.peg.2342
- eggNOG:   COG0504
- HOGENOM:   HBG597806
- OMA:   RVTMQKL
- ProtClustDB:   PRK05380
- BioCyc:   DARO159087:DARO_2366-MONOMER
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 60018; Mature: 59887

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 378-378 ACT_SITE 516-516 ACT_SITE 518-518

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKYVFVTGGVVSSLGKGIAAASLGAILESRGIKVTHLKLDPYINVDPGTMSPFQHGEVF
CCEEEEEECHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTSAKMAKRNNFTTGQIYDSVLKKERRGEYLGKTVQVIPHITD
EECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
EIKDRIKAGAEGADVAIVEVGGTVGDIESLPFLEAIRQMGIMEGRNNVCFMHLTLLPYIP
HHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCC
TAGELKTKPTQHSVKELREIGIQPDILLCRADRSIPVEERRKIALFCNVMPEAVIECLDA
CCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHCCEEEEEEHHHHHHHHHHHCC
DSIYKIPGMLHEQMLDEIVCHKLNILAKAADLTVWENLIVALEHPLHQVKIAFVGKYVDL
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEHHHHHHHHHH
TESYKSLIEAIKHAGIHTRSQVNIIYLDSEDIEKNGTGVLEDLDAILVPGGFGRRGTEGK
HHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCHHHCCCEEEECCCCCCCCCCCC
IAAIRYARENKVPYLGICLGMQLAVVEFARDVAGMPGAHSTEFVQDTPYPVIGLITEWLD
EEEEEEHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCHHHHHHHHHHC
ASGKIEKRTEDSDIGGTMRLGAQVCKLGEGTLARSIYGAPEITERHRHRYEVNNTLLAKL
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHCCCHHHEEH
EEKGLIVAGRAPGTDLCEMVELPADVHPWFVGCQFHPEFTSNPRQGHPLFSSYVKAALAN
HHCCEEEECCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHC
QKAKGK
CCCCCC
>Mature Secondary Structure 
TKYVFVTGGVVSSLGKGIAAASLGAILESRGIKVTHLKLDPYINVDPGTMSPFQHGEVF
CEEEEEECHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTSAKMAKRNNFTTGQIYDSVLKKERRGEYLGKTVQVIPHITD
EECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
EIKDRIKAGAEGADVAIVEVGGTVGDIESLPFLEAIRQMGIMEGRNNVCFMHLTLLPYIP
HHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCC
TAGELKTKPTQHSVKELREIGIQPDILLCRADRSIPVEERRKIALFCNVMPEAVIECLDA
CCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHCCEEEEEEHHHHHHHHHHHCC
DSIYKIPGMLHEQMLDEIVCHKLNILAKAADLTVWENLIVALEHPLHQVKIAFVGKYVDL
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEHHHHHHHHHH
TESYKSLIEAIKHAGIHTRSQVNIIYLDSEDIEKNGTGVLEDLDAILVPGGFGRRGTEGK
HHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCHHHCCCEEEECCCCCCCCCCCC
IAAIRYARENKVPYLGICLGMQLAVVEFARDVAGMPGAHSTEFVQDTPYPVIGLITEWLD
EEEEEEHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCHHHHHHHHHHC
ASGKIEKRTEDSDIGGTMRLGAQVCKLGEGTLARSIYGAPEITERHRHRYEVNNTLLAKL
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHCCCHHHEEH
EEKGLIVAGRAPGTDLCEMVELPADVHPWFVGCQFHPEFTSNPRQGHPLFSSYVKAALAN
HHCCEEEECCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHC
QKAKGK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA