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The map label for this gene is kdsA

Identifier: 71907984

GI number: 71907984

Start: 2549256

End: 2550092

Strand: Reverse

Name: kdsA

Synonym: Daro_2365

Alternate gene names: 71907984

Gene position: 2550092-2549256 (Counterclockwise)

Preceding gene: 71907985

Following gene: 71907983

Centisome position: 56.65

GC content: 56.99

Gene sequence:

>837_bases
ATGAAGCTCTGCGGTTTCGAGGCTGGTCTTGACCAGCCTTTTTTCCTGATTGCCGGGCCATGTACTGCTGAATCCGAGCA
GCTGTGCCTCGATGTGGCTGGCCATATGAAAGAGGTTTGCGCTCGCCTTGGCATCAACTACATTTTCAAGGCGTCCTACG
ACAAGGCCAATCGCAGCTCCGGCAAGTCGGTACGCGGCCTTGGTCTTGAAGAAGGTCTGCGTATCTTCTCGGAAGTACAG
CGCCAGATTGGCGTGCCGGTGCTGACCGACGTTCATACCATCGAAGACATTCCCGCTGTAGCGGCAGTGGTCGATGTGCT
GCAGACGCCGGCTTTCCTGTGCCGCCAGACTGATTTCATTCACGCGGTTGCTACCTGCGGCAAGCCGGTGAATATCAAGA
AGGGGCAGTTTCTGGCACCGGGTGACATGAAGAACGTGGTCGACAAGGCGCGTGAAGTGAACAACGGTGCCGACAACATC
ATGGTTTGTGAGCGCGGAGCCTCTTTCGGCTACAACAATCTGGTTTCCGACATGCGTAGCTTGGCAATCATGCGCGAGAC
GGGTTGCCCGGTCGTATTCGATGCCACGCACAGCGTGCAGTTGCCGGGCGGCCAAGGGACGACTTCCGGTGGTCAGCGCG
AGTTTGTGCCGGTGCTGGCGCGAGCCGCGATCGCGGTCGGCATTTCGGGTCTGTTCATGGAAACCCACCCTTGTCCTGAA
AAGGCGTGGTCGGATGGCCCGAACAGTTGGCCGCTCGATCGCATGGAAAGCCTTTTGGCAACACTAGTTGCGCTGGATAA
GGCCGTAAAAACTGCTGGTTTTGAAGAGTTGAAATAA

Upstream 100 bases:

>100_bases
GTCAGTTCCACCCGGAATTCACCTCCAATCCGCGTCAAGGTCATCCGCTCTTCTCTTCTTACGTGAAGGCGGCGCTGGCC
AATCAAAAGGCCAAAGGCAA

Downstream 100 bases:

>100_bases
GCCGCCGCTTAATTCTGATAATAATATTTTAATTTAAGTTGTCTATTTAACCTTCTGATAATAAGGAAGAAACATGAGTT
CTATCGTTGATGTTATTGCC

Product: 2-dehydro-3-deoxyphosphooctonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase; KDO-8-phosphate synthase; KDO 8-P synthase; KDOPS; Phospho-2-dehydro-3-deoxyoctonate aldolase

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MKLCGFEAGLDQPFFLIAGPCTAESEQLCLDVAGHMKEVCARLGINYIFKASYDKANRSSGKSVRGLGLEEGLRIFSEVQ
RQIGVPVLTDVHTIEDIPAVAAVVDVLQTPAFLCRQTDFIHAVATCGKPVNIKKGQFLAPGDMKNVVDKAREVNNGADNI
MVCERGASFGYNNLVSDMRSLAIMRETGCPVVFDATHSVQLPGGQGTTSGGQREFVPVLARAAIAVGISGLFMETHPCPE
KAWSDGPNSWPLDRMESLLATLVALDKAVKTAGFEELK

Sequences:

>Translated_278_residues
MKLCGFEAGLDQPFFLIAGPCTAESEQLCLDVAGHMKEVCARLGINYIFKASYDKANRSSGKSVRGLGLEEGLRIFSEVQ
RQIGVPVLTDVHTIEDIPAVAAVVDVLQTPAFLCRQTDFIHAVATCGKPVNIKKGQFLAPGDMKNVVDKAREVNNGADNI
MVCERGASFGYNNLVSDMRSLAIMRETGCPVVFDATHSVQLPGGQGTTSGGQREFVPVLARAAIAVGISGLFMETHPCPE
KAWSDGPNSWPLDRMESLLATLVALDKAVKTAGFEELK
>Mature_278_residues
MKLCGFEAGLDQPFFLIAGPCTAESEQLCLDVAGHMKEVCARLGINYIFKASYDKANRSSGKSVRGLGLEEGLRIFSEVQ
RQIGVPVLTDVHTIEDIPAVAAVVDVLQTPAFLCRQTDFIHAVATCGKPVNIKKGQFLAPGDMKNVVDKAREVNNGADNI
MVCERGASFGYNNLVSDMRSLAIMRETGCPVVFDATHSVQLPGGQGTTSGGQREFVPVLARAAIAVGISGLFMETHPCPE
KAWSDGPNSWPLDRMESLLATLVALDKAVKTAGFEELK

Specific function: Synthesis Of Kdo 8-P Which Is Required For Lipid A Maturation And Cellular Growth. [C]

COG id: COG2877

COG function: function code M; 3-deoxy-D-manno-octulosonic acid (KDO) 8-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsA family

Homologues:

Organism=Escherichia coli, GI1787466, Length=269, Percent_Identity=49.4423791821561, Blast_Score=259, Evalue=1e-70,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KDSA_DECAR (Q47DI0)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_285571.1
- HSSP:   O66496
- ProteinModelPortal:   Q47DI0
- SMR:   Q47DI0
- STRING:   Q47DI0
- GeneID:   3566047
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_2365
- NMPDR:   fig|159087.4.peg.2341
- eggNOG:   COG2877
- HOGENOM:   HBG575990
- OMA:   PDPSKAM
- ProtClustDB:   PRK05198
- BioCyc:   DARO159087:DARO_2365-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00056
- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006269
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21057:SF2
- TIGRFAMs:   TIGR01362

Pfam domain/function: PF00793 DAHP_synth_1

EC number: =2.5.1.55

Molecular weight: Translated: 29844; Mature: 29844

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLCGFEAGLDQPFFLIAGPCTAESEQLCLDVAGHMKEVCARLGINYIFKASYDKANRSS
CCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCCC
GKSVRGLGLEEGLRIFSEVQRQIGVPVLTDVHTIEDIPAVAAVVDVLQTPAFLCRQTDFI
CCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
HAVATCGKPVNIKKGQFLAPGDMKNVVDKAREVNNGADNIMVCERGASFGYNNLVSDMRS
HHHHHCCCCCCCCCCCEECCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHH
LAIMRETGCPVVFDATHSVQLPGGQGTTSGGQREFVPVLARAAIAVGISGLFMETHPCPE
HHHHHHCCCCEEEECCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCH
KAWSDGPNSWPLDRMESLLATLVALDKAVKTAGFEELK
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCC
>Mature Secondary Structure
MKLCGFEAGLDQPFFLIAGPCTAESEQLCLDVAGHMKEVCARLGINYIFKASYDKANRSS
CCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCCC
GKSVRGLGLEEGLRIFSEVQRQIGVPVLTDVHTIEDIPAVAAVVDVLQTPAFLCRQTDFI
CCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
HAVATCGKPVNIKKGQFLAPGDMKNVVDKAREVNNGADNIMVCERGASFGYNNLVSDMRS
HHHHHCCCCCCCCCCCEECCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHH
LAIMRETGCPVVFDATHSVQLPGGQGTTSGGQREFVPVLARAAIAVGISGLFMETHPCPE
HHHHHHCCCCEEEECCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCH
KAWSDGPNSWPLDRMESLLATLVALDKAVKTAGFEELK
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA