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The map label for this gene is eno

Identifier: 71907983

GI number: 71907983

Start: 2547899

End: 2549182

Strand: Reverse

Name: eno

Synonym: Daro_2364

Alternate gene names: 71907983

Gene position: 2549182-2547899 (Counterclockwise)

Preceding gene: 71907984

Following gene: 71907982

Centisome position: 56.63

GC content: 57.32

Gene sequence:

>1284_bases
ATGAGTTCTATCGTTGATGTTATTGCCCGTGAAATTCTGGACTCCCGTGGCAATCCCACTGTCGAAGCTGACGTTCTGCT
CGAATCCGGCGTCATGGGCCGTGCTGCCGTCCCGTCCGGCGCCTCGACCGGTACCCGCGAAGCCATCGAACTGCGTGATG
GTGATGCCAGCCGTTATCTCGGCAAGGGCGTCATGCAGGCTGTCGAGAACGTCAACACCGAGATCTCCGAAGCCATCATC
GGCCTTGACGCACAAGAGCAGGCTTTCATCGATCAGACCATGATTGACCTCGACGGTACGGACAACAAGTCCCGCCTCGG
TGCCAACGCCATTCTGGCCGTGTCGATGGCTGTCGCCAAGGCTGCTGCCGAAGAGTCCGGTCTGCCGCTCTATCGCTACT
TCGGTGGCATGAGCCCGATGCAGATGCCGGTGCCGATGATGAACATCATCAACGGTGGTGAGCACGCCAACAACAGCCTG
GATATTCAGGAATTCATGGTGATGCCGGTTGGTGCTGCCAATATTCGTGAAGCGATCCGTTGCGGTGCAGAAATTTTTCA
TGCGCTGAAGAAGCTGCTGAACAAGAAGGGTCATTCGACCGCCGTCGGCGATGAAGGTGGCTTTGCCCCGAATCTGGGTA
GCCATGCCGAAGCCCTGCAGATCATCATGGAAGCCATCGAAATTGCCGGTTATGTGCCGGGCCAGGATGTGCTGCTGGCG
CTCGACTGCGCTGCTTCCGAGTTCTACAAGGACGGCAAATACAAGTTGGCTGGTGAGGGACTGGAGCTGACCTCGGCCCA
GTTCGTCGATTACCTGGCCAACCTGGCTGACCAGTTCCCGATCGTTTCGATCGAAGACGGCATGTCCGAAGCCGACTGGG
ATGGCTGGAAGCTGCTGACCGATCGCCTCGGCGACAAGGTACAGATCGTTGGTGATGACATCTTCGTCACCAACACCAAG
ATCTTCAAGGAAGGTATCAAGAAGGGGATCGGCAATTCGATCCTGATCAAGATCAACCAGATCGGCACCCTGTCCGAAAC
CTTTGCTGCCGTTGAAATGGCCAAGCGTGCCGGTTACACCGCCGTGATCTCGCATCGCTCCGGTGAAACCGAAGACAGCA
CCATCGCCGACATCGCCGTTGGCCTGAACGCCGGTCAGATCAAGACCGGTTCGCTGTCGCGTTCCGACCGCATTGCCAAG
TACAACCAGCTGATCCGTATCGAGGAAGATCTGGGCGATACCGCTTCCTACCCGGGCCGCGAAACCTTCTACAACCTGCG
TTAA

Upstream 100 bases:

>100_bases
ACTGCTGGTTTTGAAGAGTTGAAATAAGCCGCCGCTTAATTCTGATAATAATATTTTAATTTAAGTTGTCTATTTAACCT
TCTGATAATAAGGAAGAAAC

Downstream 100 bases:

>100_bases
TCACGCATGCGTTGGCTGACGGTCGGCCTCCTCGCAGCCATCGGCCTGCTCCAATACCCCCTGTGGGTGGGTAAGGGCGG
CTGGCTGAAGGTATGGGAGT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSSIVDVIAREILDSRGNPTVEADVLLESGVMGRAAVPSGASTGTREAIELRDGDASRYLGKGVMQAVENVNTEISEAII
GLDAQEQAFIDQTMIDLDGTDNKSRLGANAILAVSMAVAKAAAEESGLPLYRYFGGMSPMQMPVPMMNIINGGEHANNSL
DIQEFMVMPVGAANIREAIRCGAEIFHALKKLLNKKGHSTAVGDEGGFAPNLGSHAEALQIIMEAIEIAGYVPGQDVLLA
LDCAASEFYKDGKYKLAGEGLELTSAQFVDYLANLADQFPIVSIEDGMSEADWDGWKLLTDRLGDKVQIVGDDIFVTNTK
IFKEGIKKGIGNSILIKINQIGTLSETFAAVEMAKRAGYTAVISHRSGETEDSTIADIAVGLNAGQIKTGSLSRSDRIAK
YNQLIRIEEDLGDTASYPGRETFYNLR

Sequences:

>Translated_427_residues
MSSIVDVIAREILDSRGNPTVEADVLLESGVMGRAAVPSGASTGTREAIELRDGDASRYLGKGVMQAVENVNTEISEAII
GLDAQEQAFIDQTMIDLDGTDNKSRLGANAILAVSMAVAKAAAEESGLPLYRYFGGMSPMQMPVPMMNIINGGEHANNSL
DIQEFMVMPVGAANIREAIRCGAEIFHALKKLLNKKGHSTAVGDEGGFAPNLGSHAEALQIIMEAIEIAGYVPGQDVLLA
LDCAASEFYKDGKYKLAGEGLELTSAQFVDYLANLADQFPIVSIEDGMSEADWDGWKLLTDRLGDKVQIVGDDIFVTNTK
IFKEGIKKGIGNSILIKINQIGTLSETFAAVEMAKRAGYTAVISHRSGETEDSTIADIAVGLNAGQIKTGSLSRSDRIAK
YNQLIRIEEDLGDTASYPGRETFYNLR
>Mature_426_residues
SSIVDVIAREILDSRGNPTVEADVLLESGVMGRAAVPSGASTGTREAIELRDGDASRYLGKGVMQAVENVNTEISEAIIG
LDAQEQAFIDQTMIDLDGTDNKSRLGANAILAVSMAVAKAAAEESGLPLYRYFGGMSPMQMPVPMMNIINGGEHANNSLD
IQEFMVMPVGAANIREAIRCGAEIFHALKKLLNKKGHSTAVGDEGGFAPNLGSHAEALQIIMEAIEIAGYVPGQDVLLAL
DCAASEFYKDGKYKLAGEGLELTSAQFVDYLANLADQFPIVSIEDGMSEADWDGWKLLTDRLGDKVQIVGDDIFVTNTKI
FKEGIKKGIGNSILIKINQIGTLSETFAAVEMAKRAGYTAVISHRSGETEDSTIADIAVGLNAGQIKTGSLSRSDRIAKY
NQLIRIEEDLGDTASYPGRETFYNLR

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=437, Percent_Identity=50.5720823798627, Blast_Score=424, Evalue=1e-119,
Organism=Homo sapiens, GI4503571, Length=430, Percent_Identity=50, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI301897477, Length=427, Percent_Identity=50.3512880562061, Blast_Score=413, Evalue=1e-115,
Organism=Homo sapiens, GI301897469, Length=427, Percent_Identity=50.3512880562061, Blast_Score=413, Evalue=1e-115,
Organism=Homo sapiens, GI301897479, Length=425, Percent_Identity=45.6470588235294, Blast_Score=356, Evalue=2e-98,
Organism=Homo sapiens, GI169201331, Length=351, Percent_Identity=26.4957264957265, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI169201757, Length=351, Percent_Identity=26.4957264957265, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI239744207, Length=351, Percent_Identity=26.4957264957265, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1789141, Length=426, Percent_Identity=68.3098591549296, Blast_Score=579, Evalue=1e-166,
Organism=Caenorhabditis elegans, GI71995829, Length=437, Percent_Identity=49.1990846681922, Blast_Score=408, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17536383, Length=437, Percent_Identity=49.1990846681922, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI32563855, Length=197, Percent_Identity=43.6548223350254, Blast_Score=169, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6321693, Length=438, Percent_Identity=48.4018264840183, Blast_Score=400, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=49.1916859122402, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=49.1916859122402, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=48.9607390300231, Blast_Score=392, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6321968, Length=438, Percent_Identity=48.8584474885845, Blast_Score=376, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580918, Length=431, Percent_Identity=50.1160092807425, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580916, Length=431, Percent_Identity=50.1160092807425, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580920, Length=431, Percent_Identity=50.1160092807425, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580914, Length=431, Percent_Identity=50.1160092807425, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI281360527, Length=431, Percent_Identity=50.1160092807425, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI17137654, Length=431, Percent_Identity=50.1160092807425, Blast_Score=387, Evalue=1e-108,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_DECAR (Q47DI1)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_285570.1
- HSSP:   P0A6P9
- ProteinModelPortal:   Q47DI1
- SMR:   Q47DI1
- STRING:   Q47DI1
- GeneID:   3566046
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_2364
- NMPDR:   fig|159087.4.peg.2340
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- BioCyc:   DARO159087:DARO_2364-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45720; Mature: 45589

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 337-337 BINDING 155-155 BINDING 164-164 BINDING 285-285 BINDING 312-312 BINDING 337-337 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIVDVIAREILDSRGNPTVEADVLLESGVMGRAAVPSGASTGTREAIELRDGDASRYL
CHHHHHHHHHHHHHCCCCCCEEHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHH
GKGVMQAVENVNTEISEAIIGLDAQEQAFIDQTMIDLDGTDNKSRLGANAILAVSMAVAK
HHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHEECCCCCCHHHHCCHHHHHHHHHHHH
AAAEESGLPLYRYFGGMSPMQMPVPMMNIINGGEHANNSLDIQEFMVMPVGAANIREAIR
HHHHHCCCCHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHCCCCCHHHHHHHHH
CGAEIFHALKKLLNKKGHSTAVGDEGGFAPNLGSHAEALQIIMEAIEIAGYVPGQDVLLA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
LDCAASEFYKDGKYKLAGEGLELTSAQFVDYLANLADQFPIVSIEDGMSEADWDGWKLLT
EEHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHH
DRLGDKVQIVGDDIFVTNTKIFKEGIKKGIGNSILIKINQIGTLSETFAAVEMAKRAGYT
HHCCCEEEEEECCEEEECHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCE
AVISHRSGETEDSTIADIAVGLNAGQIKTGSLSRSDRIAKYNQLIRIEEDLGDTASYPGR
EEEECCCCCCCCCHHHHHEECCCCCCEECCCCCCHHHHHHHHHHEEEHHHCCCCCCCCCC
ETFYNLR
CCCCCCC
>Mature Secondary Structure 
SSIVDVIAREILDSRGNPTVEADVLLESGVMGRAAVPSGASTGTREAIELRDGDASRYL
HHHHHHHHHHHHHCCCCCCEEHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHH
GKGVMQAVENVNTEISEAIIGLDAQEQAFIDQTMIDLDGTDNKSRLGANAILAVSMAVAK
HHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHEECCCCCCHHHHCCHHHHHHHHHHHH
AAAEESGLPLYRYFGGMSPMQMPVPMMNIINGGEHANNSLDIQEFMVMPVGAANIREAIR
HHHHHCCCCHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHCCCCCHHHHHHHHH
CGAEIFHALKKLLNKKGHSTAVGDEGGFAPNLGSHAEALQIIMEAIEIAGYVPGQDVLLA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
LDCAASEFYKDGKYKLAGEGLELTSAQFVDYLANLADQFPIVSIEDGMSEADWDGWKLLT
EEHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHH
DRLGDKVQIVGDDIFVTNTKIFKEGIKKGIGNSILIKINQIGTLSETFAAVEMAKRAGYT
HHCCCEEEEEECCEEEECHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCE
AVISHRSGETEDSTIADIAVGLNAGQIKTGSLSRSDRIAKYNQLIRIEEDLGDTASYPGR
EEEECCCCCCCCCHHHHHEECCCCCCEECCCCCCHHHHHHHHHHEEEHHHCCCCCCCCCC
ETFYNLR
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA