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The map label for this gene is livM [H]

Identifier: 71907977

GI number: 71907977

Start: 2542871

End: 2543917

Strand: Reverse

Name: livM [H]

Synonym: Daro_2358

Alternate gene names: 71907977

Gene position: 2543917-2542871 (Counterclockwise)

Preceding gene: 71907978

Following gene: 71907976

Centisome position: 56.52

GC content: 61.99

Gene sequence:

>1047_bases
ATGCGTATCGGTACCGCCAAGCAAAGCTACGCCCACGACGAGTCGCTGTTCGACACCAGGACCCAGTGGATCTGGCTGGG
CCTCCTCGGCGTCAGTCTGCTGGTCTTCCCCTTCGTCGTCGATGATTACTGGCTCTATCTGGCCTGTCTGGTTGCGATCA
ACATCGCCAGCGCCACCGGGCTGAACATCCTGACCGGCTATACCGGCCTGGTCAGCCTTGGGCAGGCTGCTTTCATGGGG
CTGGGCGCTTACACGGTGGCCATTCTGGAACAGCGTTTCGGTACGCCGGCCGTGCTTAATCTGGTGGCCGGCGGCGCCGT
TGCCATGCTGGTCGGCGTTGTCGTCGGCATTCCATCCTTGCGGGTCAAGGGGCTGTACCTGGCCATTTCGACCATTGCGG
CATCGTTCATCCTGCACTTCGTCTTTGCCAACTGGAGCAGCCTGACCGGTGGCACAGGCGGGATGACAGTGCCGCCGGCC
AACTTCTTCGGTATTCCGCTGGATACATCGTTCCGCCTGTACTGGCTGATCGTCCCGGTGGCGCTGACCATGCTGGTCTG
TGCCGCGAACCTGTTCCGCACGCGGATCGGCCGGGCCTTCATCGCCATCCGTGATCGCGACATCTCGGCTGAGGTGCTGG
GCATTCCGCTGCTTCGCTACAAGCTGACCTCATTTGCGCTGTCGTCGTTTTACGCCGGCGTCGCTGGTGGCTTGTGGGCC
TACTTCTTCCGGGTCGTTACGCCGGAAAGCTTCCCGCTGATGATGTCCATCTTCTTCCTGGCCGCGGTGATCGTCGGCGG
CATGGGCACCATCCTTGGCGGCATCCTCGGTGCCATCTTCATGACCATGGTGCCGGAAGTCCTCAAGCTGATCGTTGGCT
GGCTGCCGCTGGGCAGTGAGGGCGCCGTGATTCTTTCGCCGGTGCGGACCATCGTGTTTGGTCTGCTCATCGTCGGATTC
CTGATCTTCGAGCCCCTTGGGCTCCAGGAACTGTGGCGCCGTGTGCGCCGCTTCTTTCACCTGTGGCCTTTCCGGGCTCA
GGGATAA

Upstream 100 bases:

>100_bases
GTGGCGAATTCAAACTGCTGGCCACCTTCTCCACCCTGATCCTGGTGCTGATGATCCGTCCCTACGGTCTGTTCGGCACC
CATGAAATCGAGAGGCTCTG

Downstream 100 bases:

>100_bases
CAACGGAGGAGACACCGTGGAACAAAAGAAAATCATCAAATGGCTGGGTTCGCTGGCTGCTGCTGCCAGCCTGATCGCTG
CACCGCTGGCGCAGGCCGAG

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MRIGTAKQSYAHDESLFDTRTQWIWLGLLGVSLLVFPFVVDDYWLYLACLVAINIASATGLNILTGYTGLVSLGQAAFMG
LGAYTVAILEQRFGTPAVLNLVAGGAVAMLVGVVVGIPSLRVKGLYLAISTIAASFILHFVFANWSSLTGGTGGMTVPPA
NFFGIPLDTSFRLYWLIVPVALTMLVCAANLFRTRIGRAFIAIRDRDISAEVLGIPLLRYKLTSFALSSFYAGVAGGLWA
YFFRVVTPESFPLMMSIFFLAAVIVGGMGTILGGILGAIFMTMVPEVLKLIVGWLPLGSEGAVILSPVRTIVFGLLIVGF
LIFEPLGLQELWRRVRRFFHLWPFRAQG

Sequences:

>Translated_348_residues
MRIGTAKQSYAHDESLFDTRTQWIWLGLLGVSLLVFPFVVDDYWLYLACLVAINIASATGLNILTGYTGLVSLGQAAFMG
LGAYTVAILEQRFGTPAVLNLVAGGAVAMLVGVVVGIPSLRVKGLYLAISTIAASFILHFVFANWSSLTGGTGGMTVPPA
NFFGIPLDTSFRLYWLIVPVALTMLVCAANLFRTRIGRAFIAIRDRDISAEVLGIPLLRYKLTSFALSSFYAGVAGGLWA
YFFRVVTPESFPLMMSIFFLAAVIVGGMGTILGGILGAIFMTMVPEVLKLIVGWLPLGSEGAVILSPVRTIVFGLLIVGF
LIFEPLGLQELWRRVRRFFHLWPFRAQG
>Mature_348_residues
MRIGTAKQSYAHDESLFDTRTQWIWLGLLGVSLLVFPFVVDDYWLYLACLVAINIASATGLNILTGYTGLVSLGQAAFMG
LGAYTVAILEQRFGTPAVLNLVAGGAVAMLVGVVVGIPSLRVKGLYLAISTIAASFILHFVFANWSSLTGGTGGMTVPPA
NFFGIPLDTSFRLYWLIVPVALTMLVCAANLFRTRIGRAFIAIRDRDISAEVLGIPLLRYKLTSFALSSFYAGVAGGLWA
YFFRVVTPESFPLMMSIFFLAAVIVGGMGTILGGILGAIFMTMVPEVLKLIVGWLPLGSEGAVILSPVRTIVFGLLIVGF
LIFEPLGLQELWRRVRRFFHLWPFRAQG

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=277, Percent_Identity=33.9350180505415, Blast_Score=126, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 37761; Mature: 37761

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGTAKQSYAHDESLFDTRTQWIWLGLLGVSLLVFPFVVDDYWLYLACLVAINIASATG
CCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LNILTGYTGLVSLGQAAFMGLGAYTVAILEQRFGTPAVLNLVAGGAVAMLVGVVVGIPSL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHCCCCH
RVKGLYLAISTIAASFILHFVFANWSSLTGGTGGMTVPPANFFGIPLDTSFRLYWLIVPV
HHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHH
ALTMLVCAANLFRTRIGRAFIAIRDRDISAEVLGIPLLRYKLTSFALSSFYAGVAGGLWA
HHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YFFRVVTPESFPLMMSIFFLAAVIVGGMGTILGGILGAIFMTMVPEVLKLIVGWLPLGSE
HHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GAVILSPVRTIVFGLLIVGFLIFEPLGLQELWRRVRRFFHLWPFRAQG
CCEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRIGTAKQSYAHDESLFDTRTQWIWLGLLGVSLLVFPFVVDDYWLYLACLVAINIASATG
CCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LNILTGYTGLVSLGQAAFMGLGAYTVAILEQRFGTPAVLNLVAGGAVAMLVGVVVGIPSL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHCCCCH
RVKGLYLAISTIAASFILHFVFANWSSLTGGTGGMTVPPANFFGIPLDTSFRLYWLIVPV
HHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHH
ALTMLVCAANLFRTRIGRAFIAIRDRDISAEVLGIPLLRYKLTSFALSSFYAGVAGGLWA
HHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YFFRVVTPESFPLMMSIFFLAAVIVGGMGTILGGILGAIFMTMVPEVLKLIVGWLPLGSE
HHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GAVILSPVRTIVFGLLIVGFLIFEPLGLQELWRRVRRFFHLWPFRAQG
CCEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]