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The map label for this gene is czcB [H]

Identifier: 71907873

GI number: 71907873

Start: 2439146

End: 2440342

Strand: Reverse

Name: czcB [H]

Synonym: Daro_2253

Alternate gene names: 71907873

Gene position: 2440342-2439146 (Counterclockwise)

Preceding gene: 71907874

Following gene: 71907872

Centisome position: 54.22

GC content: 62.24

Gene sequence:

>1197_bases
ATGACCCGCATCCTCCCGACAACCCTCTTGGCCCTCTCGCTGGTGCTGGTCGCCTGCGGTCGTAACGCGCCTGAGAGCAG
CACCGTGACGGAAAAGCCCGCCGCTGCCCCAGAAAAAACTGCTACCGGCGAAAAAGCAGCACCGACCGAAGCGCCGGAAG
CCGGCCAGTTACATCTGACGGAGGCGGAAATCAAGGCCGCCGGCATTCGCACCGAAGACGCCACTGAAGCGGAGGTTCAC
GAGCGGATCGCCGTAACGGCAACCATCCAGGCCAATCAGGATCGGCTGGCCCATGTTGCCCCACGGGTGCCAGGCCGCAT
CGTCAGCATTGCCGCCAAATTGGGGGATCGGGTTAAGGCGGGGCAGGCCCTGGCCAGCCTGGACAGCATCGAACTGGGTG
AAGCCCACTCCACCTACCTCCAGGCCGACAGCCAGTACCGACTGGCCCAAGCCGATTTCGAGCGGGCCGAAAAGCTTTAC
GCCGACCAGATCGTGCCGCAAAAGGACTACCTGCGCAGCCGTGCAGAGCACGAGAAAGCCCGCGCCAACTTGCGGGCGTC
AACCGACAAGCTGCGTCTGATGGGCGTGACGCCGGCCCGCTCGGAAACCGCCATGTCGGTGTTCCCGGTCAATGCGCCAT
TTGCCGGAACAGTGATCGAAAAGACAGCGGTGTTAGGCGAACTCGCCCAGCCGGACAAGGCACTGTTCACCGTGGCCGAC
CTATCCGTGGTCTGGATCGAGGCCAGCCTGTTCGAGAAAGACCTCGGAAAGGTAAAAACCGGTGCCACGGTTGGCGTGAC
AGTCGCTGCTTATCCGGACGAAGTGTTCAAAGGGCGTCTGACCTACATCAGCAGCGTCATGGACAAGGAAAGCCGCACGA
TCAAGGCACGGGTGGAAGTCGCTAATGCCGACGGTCGCCTGAAGCCCGACATGTTCGCCAACGCAGCCATCGACACGACG
ACGGCGGGCAAGGCGCTGACGGTGCCCTCGGGGGCCGTGCTTTTGCTGGAAGGCAAGAAAGCGGTCTTTGTTCGCGAGAA
GGAAGGCTTCGAGAAGCGAGAAGTCGAACTCGGGGACAACCTCGGTGGCCGTTTCGTGATCAAGAGCGGCATCGAAGCCG
GCGAAGCCGTGGTGGTCGACGGTGCCTATGCGCTCAAGGCGCGGCTCCTGAAATCCAAAATCGGCGATCACGACTAA

Upstream 100 bases:

>100_bases
ACCTCGATGCCCTCGGCGACTTTATCCAGACCCGCATTGCCCTAGAGCAAACGGCCGGCTGGCCCGTCGCTCAAGCCAAC
CCATCCCCCGAGAAAAACAA

Downstream 100 bases:

>100_bases
GGAACGGCCATGCTCAACAAGATCGTCGATATCTCCCTGCGCTACAAGCTGCTGGTTCTGGTGGCTCTGGCGCTGATCGT
ACTGCTCGGGGCACGAGGCT

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MTRILPTTLLALSLVLVACGRNAPESSTVTEKPAAAPEKTATGEKAAPTEAPEAGQLHLTEAEIKAAGIRTEDATEAEVH
ERIAVTATIQANQDRLAHVAPRVPGRIVSIAAKLGDRVKAGQALASLDSIELGEAHSTYLQADSQYRLAQADFERAEKLY
ADQIVPQKDYLRSRAEHEKARANLRASTDKLRLMGVTPARSETAMSVFPVNAPFAGTVIEKTAVLGELAQPDKALFTVAD
LSVVWIEASLFEKDLGKVKTGATVGVTVAAYPDEVFKGRLTYISSVMDKESRTIKARVEVANADGRLKPDMFANAAIDTT
TAGKALTVPSGAVLLLEGKKAVFVREKEGFEKREVELGDNLGGRFVIKSGIEAGEAVVVDGAYALKARLLKSKIGDHD

Sequences:

>Translated_398_residues
MTRILPTTLLALSLVLVACGRNAPESSTVTEKPAAAPEKTATGEKAAPTEAPEAGQLHLTEAEIKAAGIRTEDATEAEVH
ERIAVTATIQANQDRLAHVAPRVPGRIVSIAAKLGDRVKAGQALASLDSIELGEAHSTYLQADSQYRLAQADFERAEKLY
ADQIVPQKDYLRSRAEHEKARANLRASTDKLRLMGVTPARSETAMSVFPVNAPFAGTVIEKTAVLGELAQPDKALFTVAD
LSVVWIEASLFEKDLGKVKTGATVGVTVAAYPDEVFKGRLTYISSVMDKESRTIKARVEVANADGRLKPDMFANAAIDTT
TAGKALTVPSGAVLLLEGKKAVFVREKEGFEKREVELGDNLGGRFVIKSGIEAGEAVVVDGAYALKARLLKSKIGDHD
>Mature_397_residues
TRILPTTLLALSLVLVACGRNAPESSTVTEKPAAAPEKTATGEKAAPTEAPEAGQLHLTEAEIKAAGIRTEDATEAEVHE
RIAVTATIQANQDRLAHVAPRVPGRIVSIAAKLGDRVKAGQALASLDSIELGEAHSTYLQADSQYRLAQADFERAEKLYA
DQIVPQKDYLRSRAEHEKARANLRASTDKLRLMGVTPARSETAMSVFPVNAPFAGTVIEKTAVLGELAQPDKALFTVADL
SVVWIEASLFEKDLGKVKTGATVGVTVAAYPDEVFKGRLTYISSVMDKESRTIKARVEVANADGRLKPDMFANAAIDTTT
AGKALTVPSGAVLLLEGKKAVFVREKEGFEKREVELGDNLGGRFVIKSGIEAGEAVVVDGAYALKARLLKSKIGDHD

Specific function: CzcA and CzcB together would act in zinc efflux nearly as effectively as the complete czc efflux system (CzcABC). The CzcB protein is thought to funnel zinc cations to the CzcA transport protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=315, Percent_Identity=26.6666666666667, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1789929, Length=321, Percent_Identity=23.98753894081, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789665, Length=316, Percent_Identity=25, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786787, Length=347, Percent_Identity=23.6311239193084, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787013, Length=285, Percent_Identity=23.1578947368421, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005695
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42451; Mature: 42320

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRILPTTLLALSLVLVACGRNAPESSTVTEKPAAAPEKTATGEKAAPTEAPEAGQLHLT
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
EAEIKAAGIRTEDATEAEVHERIAVTATIQANQDRLAHVAPRVPGRIVSIAAKLGDRVKA
HHHHHHCCCCCCCCCHHHHHCEEEEEEEEECCCHHHHHHCCCCCCHHHHHHHHHCCHHHH
GQALASLDSIELGEAHSTYLQADSQYRLAQADFERAEKLYADQIVPQKDYLRSRAEHEKA
HHHHHHHCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
RANLRASTDKLRLMGVTPARSETAMSVFPVNAPFAGTVIEKTAVLGELAQPDKALFTVAD
HHHHHCCCCCEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHEEEEEC
LSVVWIEASLFEKDLGKVKTGATVGVTVAAYPDEVFKGRLTYISSVMDKESRTIKARVEV
EEEEEEEHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEEE
ANADGRLKPDMFANAAIDTTTAGKALTVPSGAVLLLEGKKAVFVREKEGFEKREVELGDN
ECCCCCCCCHHHCCCEECCCCCCCEEECCCCEEEEEECCEEEEEECCCCCCHHHHCCCCC
LGGRFVIKSGIEAGEAVVVDGAYALKARLLKSKIGDHD
CCCEEEEECCCCCCCEEEEECHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TRILPTTLLALSLVLVACGRNAPESSTVTEKPAAAPEKTATGEKAAPTEAPEAGQLHLT
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
EAEIKAAGIRTEDATEAEVHERIAVTATIQANQDRLAHVAPRVPGRIVSIAAKLGDRVKA
HHHHHHCCCCCCCCCHHHHHCEEEEEEEEECCCHHHHHHCCCCCCHHHHHHHHHCCHHHH
GQALASLDSIELGEAHSTYLQADSQYRLAQADFERAEKLYADQIVPQKDYLRSRAEHEKA
HHHHHHHCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
RANLRASTDKLRLMGVTPARSETAMSVFPVNAPFAGTVIEKTAVLGELAQPDKALFTVAD
HHHHHCCCCCEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHEEEEEC
LSVVWIEASLFEKDLGKVKTGATVGVTVAAYPDEVFKGRLTYISSVMDKESRTIKARVEV
EEEEEEEHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEEE
ANADGRLKPDMFANAAIDTTTAGKALTVPSGAVLLLEGKKAVFVREKEGFEKREVELGDN
ECCCCCCCCHHHCCCEECCCCCCCEEECCCCEEEEEECCEEEEEECCCCCCHHHHCCCCC
LGGRFVIKSGIEAGEAVVVDGAYALKARLLKSKIGDHD
CCCEEEEECCCCCCCEEEEECHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8829543 [H]