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The map label for this gene is tmk

Identifier: 71907819

GI number: 71907819

Start: 2372776

End: 2373390

Strand: Reverse

Name: tmk

Synonym: Daro_2197

Alternate gene names: 71907819

Gene position: 2373390-2372776 (Counterclockwise)

Preceding gene: 71907820

Following gene: 71907818

Centisome position: 52.73

GC content: 56.26

Gene sequence:

>615_bases
GTGAAAGGCAAATTCATCACTTTCGAAGGCATCGACGGTGCCGGCAAAAGTAGCCACGTCGAATGGCTGGCTGGATTGCT
GCGCCAACGCGGTATCGTTGTTCATGTAACGCGCGAGCCGGGCGGCACCGAATTGGGTGAAAAGCTGCGCGAATTGTTGC
TCAACGAACCGATGCATCTGGAAACCGAAACGCTGCTGATGTTCGCTGCTCGTCGTGAACACCTGGCGCGGCTGATCGAG
CCGGCCCTGGCGCGGGGTGAATGGGTGGTCTGCGACCGCTTTAGCGATGCCACCTATGCCTACCAGGGGGGAGGGCGCGG
TCTGGACCGGGCCAAGTTCCTCGCGCTGGAACATTGGGTCCATGGGCATTTGCAACCCGACCTGACGCTACTGTTCGATT
TGCCGCTGGACGTTGCGCGTGAACGCATCGTGCTGGCCAATCGTGTACTGGACAAGTTTGAGCAGGAGCGCGTCGATTTT
CATGAGCGTGTTCGTCAGGCTTATCTGGAAAGAGCGCATACCAGTCCGTCGAGAATCCGGGTAATTGACGCCAATCAGAG
CATTGAAAATATTCGCAAACAGCTTGAACAAATCGTCTCAACTGACTGTTTATGA

Upstream 100 bases:

>100_bases
ACGTCATCTATTTCGTGGCTCGAGGCGATGGCAGCAGTGAATTTTCCCGGACCCTGGACGAACACAACCGGGCGGTTAAC
AAATATCAGAGAGGTGGGAA

Downstream 100 bases:

>100_bases
ATGTAATTGAGCTTCATAGTAAGGTATGGTTAGGCTTGCAGGCAAGGCGAGAGCGCCTGCCCCATTCCTTGTTGCTGGTC
GGGCAAAAAGGGCTCGGAAA

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MKGKFITFEGIDGAGKSSHVEWLAGLLRQRGIVVHVTREPGGTELGEKLRELLLNEPMHLETETLLMFAARREHLARLIE
PALARGEWVVCDRFSDATYAYQGGGRGLDRAKFLALEHWVHGHLQPDLTLLFDLPLDVARERIVLANRVLDKFEQERVDF
HERVRQAYLERAHTSPSRIRVIDANQSIENIRKQLEQIVSTDCL

Sequences:

>Translated_204_residues
MKGKFITFEGIDGAGKSSHVEWLAGLLRQRGIVVHVTREPGGTELGEKLRELLLNEPMHLETETLLMFAARREHLARLIE
PALARGEWVVCDRFSDATYAYQGGGRGLDRAKFLALEHWVHGHLQPDLTLLFDLPLDVARERIVLANRVLDKFEQERVDF
HERVRQAYLERAHTSPSRIRVIDANQSIENIRKQLEQIVSTDCL
>Mature_204_residues
MKGKFITFEGIDGAGKSSHVEWLAGLLRQRGIVVHVTREPGGTELGEKLRELLLNEPMHLETETLLMFAARREHLARLIE
PALARGEWVVCDRFSDATYAYQGGGRGLDRAKFLALEHWVHGHLQPDLTLLFDLPLDVARERIVLANRVLDKFEQERVDF
HERVRQAYLERAHTSPSRIRVIDANQSIENIRKQLEQIVSTDCL

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family

Homologues:

Organism=Escherichia coli, GI1787340, Length=207, Percent_Identity=42.0289855072464, Blast_Score=140, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KTHY_DECAR (Q47DZ5)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_285406.1
- HSSP:   P65248
- ProteinModelPortal:   Q47DZ5
- STRING:   Q47DZ5
- GeneID:   3566652
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_2197
- NMPDR:   fig|159087.4.peg.2177
- eggNOG:   COG0125
- HOGENOM:   HBG626009
- OMA:   KGHIVIS
- ProtClustDB:   PRK00698
- BioCyc:   DARO159087:DARO_2197-MONOMER
- HAMAP:   MF_00165
- InterPro:   IPR018094
- PANTHER:   PTHR10344
- TIGRFAMs:   TIGR00041

Pfam domain/function: PF02223 Thymidylate_kin

EC number: =2.7.4.9

Molecular weight: Translated: 23368; Mature: 23368

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGKFITFEGIDGAGKSSHVEWLAGLLRQRGIVVHVTREPGGTELGEKLRELLLNEPMHL
CCCCEEEEECCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCCCC
ETETLLMFAARREHLARLIEPALARGEWVVCDRFSDATYAYQGGGRGLDRAKFLALEHWV
CHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHH
HGHLQPDLTLLFDLPLDVARERIVLANRVLDKFEQERVDFHERVRQAYLERAHTSPSRIR
HCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
VIDANQSIENIRKQLEQIVSTDCL
EEECCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKGKFITFEGIDGAGKSSHVEWLAGLLRQRGIVVHVTREPGGTELGEKLRELLLNEPMHL
CCCCEEEEECCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCCCC
ETETLLMFAARREHLARLIEPALARGEWVVCDRFSDATYAYQGGGRGLDRAKFLALEHWV
CHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHH
HGHLQPDLTLLFDLPLDVARERIVLANRVLDKFEQERVDFHERVRQAYLERAHTSPSRIR
HCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
VIDANQSIENIRKQLEQIVSTDCL
EEECCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA