Click here to switch to the map view.

The map label for this gene is holB [H]

Identifier: 71907818

GI number: 71907818

Start: 2371760

End: 2372779

Strand: Reverse

Name: holB [H]

Synonym: Daro_2196

Alternate gene names: 71907818

Gene position: 2372779-2371760 (Counterclockwise)

Preceding gene: 71907819

Following gene: 71907817

Centisome position: 52.72

GC content: 54.31

Gene sequence:

>1020_bases
ATGAATGTAATTGAGCTTCATAGTAAGGTATGGTTAGGCTTGCAGGCAAGGCGAGAGCGCCTGCCCCATTCCTTGTTGCT
GGTCGGGCAAAAAGGGCTCGGAAAATTCGAACTGGCGCGACGTTTTGCCGCCAGCCTGTTATGCGAGTCTCCTCAGTCGA
ATGGTCTGGCTTGTGGAAAATGCCTGGCCTGCAATTGGTTCGAGCAAGGAAATCATCCTGACTTTCGCCTTTTGCAGCCC
GACGCACTCGGCGAGGATGTCGAAGTCGAAGATGGCAAGAAGAAGCCGAGTCAGCAGATCACTATCGATCAGGTTCGGGC
GCTCGATGAGTTCTTCAACGTAGGAACGCACCGGGGTGGTTTGCGGATTGTCATCGTCAATCCGACCGAAGCAATGAACC
GCAGTACGGCAAACTCTCTTCTTAAAACACTTGAAGAACCGTCTGCAGATACATTGTTTTTATTGGTTTCAAGTGAGCCT
ATGCGCTTGCTGCCGACGATTCGAAGCCGTTGTCAGGTGGTTCCCGTGCCCGTTCCTTCGGCGAAGCTGGCAGGAAAGAT
ACTGGCCGACGAAGGGCTGCAGCAATCCGAGCGTTGGCTGGCGCTGGCCGGTGGTTCGCCGGTATTGGCGCTGGATCTTG
CTGCATCCGGTCAAAGCGCCTGGATTGAGCTGTTGGTGGCCAGATTGGGCAATGGCCGGAATGCAGACCCTTTGGCGATG
GCGGCTGAACTGGAAAAAGCGGTCAAGGACAGCAAAGGAAAACTTTTGCTGAAGCATGTTGTAGAAGGGGTGCAGAAATG
GCTGGTCGATTTGACGCTGGCCAAGAACGGCATGCCTGTGCGTTATTTTCTGCCGCAACAGCCCACAATTTCAGGGCTGG
CTGATATGATTCCGGCTGCGCGGCTGATACATTCTTATCGAGCCGTAATATCGCGCCGCCAGGAGGCCGAGCAGCCGCTA
AATGCGCGCCTCTTTCTGGAAGGCCTCTTTCTGGATTATCGAGCCCTGTTTGCCAATTGA

Upstream 100 bases:

>100_bases
GAGCGCATACCAGTCCGTCGAGAATCCGGGTAATTGACGCCAATCAGAGCATTGAAAATATTCGCAAACAGCTTGAACAA
ATCGTCTCAACTGACTGTTT

Downstream 100 bases:

>100_bases
TCAACGATGAGTGAAGCAAAACCCGCTCCCCAGCGCCCGAGCGTTCTCTCGCTGAACATCAATTCAAAATCCGCGCTTTA
TGCTGCGTTTATGCCGCATT

Product: DNA polymerase III, delta prime subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MNVIELHSKVWLGLQARRERLPHSLLLVGQKGLGKFELARRFAASLLCESPQSNGLACGKCLACNWFEQGNHPDFRLLQP
DALGEDVEVEDGKKKPSQQITIDQVRALDEFFNVGTHRGGLRIVIVNPTEAMNRSTANSLLKTLEEPSADTLFLLVSSEP
MRLLPTIRSRCQVVPVPVPSAKLAGKILADEGLQQSERWLALAGGSPVLALDLAASGQSAWIELLVARLGNGRNADPLAM
AAELEKAVKDSKGKLLLKHVVEGVQKWLVDLTLAKNGMPVRYFLPQQPTISGLADMIPAARLIHSYRAVISRRQEAEQPL
NARLFLEGLFLDYRALFAN

Sequences:

>Translated_339_residues
MNVIELHSKVWLGLQARRERLPHSLLLVGQKGLGKFELARRFAASLLCESPQSNGLACGKCLACNWFEQGNHPDFRLLQP
DALGEDVEVEDGKKKPSQQITIDQVRALDEFFNVGTHRGGLRIVIVNPTEAMNRSTANSLLKTLEEPSADTLFLLVSSEP
MRLLPTIRSRCQVVPVPVPSAKLAGKILADEGLQQSERWLALAGGSPVLALDLAASGQSAWIELLVARLGNGRNADPLAM
AAELEKAVKDSKGKLLLKHVVEGVQKWLVDLTLAKNGMPVRYFLPQQPTISGLADMIPAARLIHSYRAVISRRQEAEQPL
NARLFLEGLFLDYRALFAN
>Mature_339_residues
MNVIELHSKVWLGLQARRERLPHSLLLVGQKGLGKFELARRFAASLLCESPQSNGLACGKCLACNWFEQGNHPDFRLLQP
DALGEDVEVEDGKKKPSQQITIDQVRALDEFFNVGTHRGGLRIVIVNPTEAMNRSTANSLLKTLEEPSADTLFLLVSSEP
MRLLPTIRSRCQVVPVPVPSAKLAGKILADEGLQQSERWLALAGGSPVLALDLAASGQSAWIELLVARLGNGRNADPLAM
AAELEKAVKDSKGKLLLKHVVEGVQKWLVDLTLAKNGMPVRYFLPQQPTISGLADMIPAARLIHSYRAVISRRQEAEQPL
NARLFLEGLFLDYRALFAN

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=169, Percent_Identity=36.094674556213, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786676, Length=152, Percent_Identity=31.5789473684211, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37258; Mature: 37258

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVIELHSKVWLGLQARRERLPHSLLLVGQKGLGKFELARRFAASLLCESPQSNGLACGK
CCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCH
CLACNWFEQGNHPDFRLLQPDALGEDVEVEDGKKKPSQQITIDQVRALDEFFNVGTHRGG
HEEECHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
LRIVIVNPTEAMNRSTANSLLKTLEEPSADTLFLLVSSEPMRLLPTIRSRCQVVPVPVPS
EEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCEEEECCCCC
AKLAGKILADEGLQQSERWLALAGGSPVLALDLAASGQSAWIELLVARLGNGRNADPLAM
HHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHH
AAELEKAVKDSKGKLLLKHVVEGVQKWLVDLTLAKNGMPVRYFLPQQPTISGLADMIPAA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHH
RLIHSYRAVISRRQEAEQPLNARLFLEGLFLDYRALFAN
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNVIELHSKVWLGLQARRERLPHSLLLVGQKGLGKFELARRFAASLLCESPQSNGLACGK
CCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCH
CLACNWFEQGNHPDFRLLQPDALGEDVEVEDGKKKPSQQITIDQVRALDEFFNVGTHRGG
HEEECHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
LRIVIVNPTEAMNRSTANSLLKTLEEPSADTLFLLVSSEPMRLLPTIRSRCQVVPVPVPS
EEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCEEEECCCCC
AKLAGKILADEGLQQSERWLALAGGSPVLALDLAASGQSAWIELLVARLGNGRNADPLAM
HHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHH
AAELEKAVKDSKGKLLLKHVVEGVQKWLVDLTLAKNGMPVRYFLPQQPTISGLADMIPAA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHH
RLIHSYRAVISRRQEAEQPLNARLFLEGLFLDYRALFAN
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]