Click here to switch to the map view.

The map label for this gene is cls [H]

Identifier: 71907708

GI number: 71907708

Start: 2240138

End: 2241565

Strand: Reverse

Name: cls [H]

Synonym: Daro_2081

Alternate gene names: 71907708

Gene position: 2241565-2240138 (Counterclockwise)

Preceding gene: 71907710

Following gene: 71907707

Centisome position: 49.8

GC content: 59.31

Gene sequence:

>1428_bases
GTGGTTTTGCTGCCCAGCCCGGATATCGACCGCATGGTGAAGGCCTGTTCGCACTTGAGACTGGCGCTGACGTCAGCGTT
TCTGCTGTTCGTTGCCGCCGGTTGTGCGATGCTGCCCGATACCGATGCCATCATCGCCCGTCATTCCGCGCAAACGGCCC
GTTTCGAGAGTGCGCGCGGGACGATTTCGCCCGAGAAAAGCGCCGCCATCGTCGCCAGTCTGAAAAGCAGCGCTGGCGAC
ATCGATATTCTCGACAAGCAAATCGCGCTCGAGCAAGCCATCGTGGGCAGTCCGCTGATCGTCGGCAACAAGGTCATCCT
GCTTCAGGACGGCGAGGCCACCTACGCCGCCATGTTTGCCGCCATCCGTGCCGCCCGTGACCATATCAACCTGGAGTCCT
ACATCATCGAGGACGATGAAATCGGGCGCCAGTTTGCTGACTTGTTGCTGGCTCAACAGCAGCAGGGCGTGCAGGTCAAT
GTGATCTATGACAGCTTCGGCGGCATGAATACCCCAGCGGCGTATTTCGACCGCCTGCGGCAAGGCGGCATCAACGTCGT
CGAGTTCAACCCGATCAACCCGCTCATCGCCAAGGGGCAGTGGCTGTTCAACAATCGGGATCACCGGAAGCTGCTGGTCG
TCGATGGCCGGATCGCCTTCATTGGCGGCATCAATATCAGCAATGTCTATTCATCGAGCCCTGGCTCACGGCGCGCCAAG
AAGGCGCTGGGTAGCGCGATTGACTGGCGCGATACCGATTTGCAGATCGAAGGGCCAGTCGCTGGCGAGTTGCAGAAATT
GTTCATGGACACCTGGTCCAAACAGCAGGGAAAGCCGCTGGCTGGCCGAAATTACTTTCCGGAACTCAAGGCGCAGGGCA
AGGATATCGTCCGTGCCATCGGCAGTACGCCGGACGACCCCTACAGCCTGATTTACCTGACGCTGATCTCGGCCATAGGG
AACGCCGAAAGAGAGATTCACCTGACCAATGCCTATTTCGTTCCCGATCCCCGGTTGCTCAAGGCCCTCAGCGATGCGGC
CGGGCGCGGTGTCGATGTCAATCTGATCTTGCCCAGTCAGACCGATTCGAAAATTGTCTTTCATGCCGGCCGCTCGCACT
ATTCAGCGCTGCTGAAGGTGGGCGTCAAGATTCACGAGAGGGGCGGGGCGCTGCTTCATTCCAAGACGGCGCTGATCGAT
GGCGTCTGGTCATGTGTCGGTTCGACCAATCTGGACTGGCGCAGCTTTCTCGATAATGACGAGGTCAATGCCGTGATCCT
CGGTCGCGATTTCGGCTTGCAGATGAAGGCCATGATTGCCCGCGACCTTGCGGCATCGAAGGCGATCAGTGCGGAAGACT
GGGCGAACCGATCCCTGTTGTTGCGCCTCCAGGAGTGGGGCGCCAGCCTGCTGCAGAGACTGCTCTAA

Upstream 100 bases:

>100_bases
AAACCAGCAGCGACACGGTTATCGTCATCCCGCCAACCCCTGCGCCGGCCCGTTGAGCCTTCGCTAGATCGAGTCTGGGA
CAGGCGGTGAGAAGGCAATC

Downstream 100 bases:

>100_bases
GTCGAATTTGCGTTCGCACGGTTCAGCGGGGATCGAGAGCGATGATGCAAAATCTATATTCGTCTCGCTTCTGCGGCCTC
TATCTGTGTCCTGGCGTACA

Product: phospholipase D/transphosphatidylase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MVLLPSPDIDRMVKACSHLRLALTSAFLLFVAAGCAMLPDTDAIIARHSAQTARFESARGTISPEKSAAIVASLKSSAGD
IDILDKQIALEQAIVGSPLIVGNKVILLQDGEATYAAMFAAIRAARDHINLESYIIEDDEIGRQFADLLLAQQQQGVQVN
VIYDSFGGMNTPAAYFDRLRQGGINVVEFNPINPLIAKGQWLFNNRDHRKLLVVDGRIAFIGGINISNVYSSSPGSRRAK
KALGSAIDWRDTDLQIEGPVAGELQKLFMDTWSKQQGKPLAGRNYFPELKAQGKDIVRAIGSTPDDPYSLIYLTLISAIG
NAEREIHLTNAYFVPDPRLLKALSDAAGRGVDVNLILPSQTDSKIVFHAGRSHYSALLKVGVKIHERGGALLHSKTALID
GVWSCVGSTNLDWRSFLDNDEVNAVILGRDFGLQMKAMIARDLAASKAISAEDWANRSLLLRLQEWGASLLQRLL

Sequences:

>Translated_475_residues
MVLLPSPDIDRMVKACSHLRLALTSAFLLFVAAGCAMLPDTDAIIARHSAQTARFESARGTISPEKSAAIVASLKSSAGD
IDILDKQIALEQAIVGSPLIVGNKVILLQDGEATYAAMFAAIRAARDHINLESYIIEDDEIGRQFADLLLAQQQQGVQVN
VIYDSFGGMNTPAAYFDRLRQGGINVVEFNPINPLIAKGQWLFNNRDHRKLLVVDGRIAFIGGINISNVYSSSPGSRRAK
KALGSAIDWRDTDLQIEGPVAGELQKLFMDTWSKQQGKPLAGRNYFPELKAQGKDIVRAIGSTPDDPYSLIYLTLISAIG
NAEREIHLTNAYFVPDPRLLKALSDAAGRGVDVNLILPSQTDSKIVFHAGRSHYSALLKVGVKIHERGGALLHSKTALID
GVWSCVGSTNLDWRSFLDNDEVNAVILGRDFGLQMKAMIARDLAASKAISAEDWANRSLLLRLQEWGASLLQRLL
>Mature_475_residues
MVLLPSPDIDRMVKACSHLRLALTSAFLLFVAAGCAMLPDTDAIIARHSAQTARFESARGTISPEKSAAIVASLKSSAGD
IDILDKQIALEQAIVGSPLIVGNKVILLQDGEATYAAMFAAIRAARDHINLESYIIEDDEIGRQFADLLLAQQQQGVQVN
VIYDSFGGMNTPAAYFDRLRQGGINVVEFNPINPLIAKGQWLFNNRDHRKLLVVDGRIAFIGGINISNVYSSSPGSRRAK
KALGSAIDWRDTDLQIEGPVAGELQKLFMDTWSKQQGKPLAGRNYFPELKAQGKDIVRAIGSTPDDPYSLIYLTLISAIG
NAEREIHLTNAYFVPDPRLLKALSDAAGRGVDVNLILPSQTDSKIVFHAGRSHYSALLKVGVKIHERGGALLHSKTALID
GVWSCVGSTNLDWRSFLDNDEVNAVILGRDFGLQMKAMIARDLAASKAISAEDWANRSLLLRLQEWGASLLQRLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=422, Percent_Identity=31.042654028436, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1787007, Length=337, Percent_Identity=33.5311572700297, Blast_Score=162, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR015679
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 51752; Mature: 51752

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLLPSPDIDRMVKACSHLRLALTSAFLLFVAAGCAMLPDTDAIIARHSAQTARFESARG
CEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHCCCC
TISPEKSAAIVASLKSSAGDIDILDKQIALEQAIVGSPLIVGNKVILLQDGEATYAAMFA
CCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEECCEEEEEECCCHHHHHHHH
AIRAARDHINLESYIIEDDEIGRQFADLLLAQQQQGVQVNVIYDSFGGMNTPAAYFDRLR
HHHHHHHCCCCHHEEECCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHH
QGGINVVEFNPINPLIAKGQWLFNNRDHRKLLVVDGRIAFIGGINISNVYSSSPGSRRAK
HCCCEEEEECCCCCHHHCCEEEECCCCCCEEEEECCEEEEEECCCHHHCCCCCCCHHHHH
KALGSAIDWRDTDLQIEGPVAGELQKLFMDTWSKQQGKPLAGRNYFPELKAQGKDIVRAI
HHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCHHHHHHH
GSTPDDPYSLIYLTLISAIGNAEREIHLTNAYFVPDPRLLKALSDAAGRGVDVNLILPSQ
CCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHHHHCCCCEEEEEEECCC
TDSKIVFHAGRSHYSALLKVGVKIHERGGALLHSKTALIDGVWSCVGSTNLDWRSFLDND
CCCEEEEECCCHHHHHHHHHCCEEECCCCCEEEHHHHHHHHHHHHHCCCCCCHHHHCCCC
EVNAVILGRDFGLQMKAMIARDLAASKAISAEDWANRSLLLRLQEWGASLLQRLL
CCCEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHCCCHHEEHHHHHHHHHHHHHC
>Mature Secondary Structure
MVLLPSPDIDRMVKACSHLRLALTSAFLLFVAAGCAMLPDTDAIIARHSAQTARFESARG
CEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHCCCC
TISPEKSAAIVASLKSSAGDIDILDKQIALEQAIVGSPLIVGNKVILLQDGEATYAAMFA
CCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEECCEEEEEECCCHHHHHHHH
AIRAARDHINLESYIIEDDEIGRQFADLLLAQQQQGVQVNVIYDSFGGMNTPAAYFDRLR
HHHHHHHCCCCHHEEECCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHH
QGGINVVEFNPINPLIAKGQWLFNNRDHRKLLVVDGRIAFIGGINISNVYSSSPGSRRAK
HCCCEEEEECCCCCHHHCCEEEECCCCCCEEEEECCEEEEEECCCHHHCCCCCCCHHHHH
KALGSAIDWRDTDLQIEGPVAGELQKLFMDTWSKQQGKPLAGRNYFPELKAQGKDIVRAI
HHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCHHHHHHH
GSTPDDPYSLIYLTLISAIGNAEREIHLTNAYFVPDPRLLKALSDAAGRGVDVNLILPSQ
CCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHHHHCCCCEEEEEEECCC
TDSKIVFHAGRSHYSALLKVGVKIHERGGALLHSKTALIDGVWSCVGSTNLDWRSFLDND
CCCEEEEECCCHHHHHHHHHCCEEECCCCCEEEHHHHHHHHHHHHHCCCCCCHHHHCCCC
EVNAVILGRDFGLQMKAMIARDLAASKAISAEDWANRSLLLRLQEWGASLLQRLL
CCCEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHCCCHHEEHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA