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The map label for this gene is cho [H]

Identifier: 71907536

GI number: 71907536

Start: 2049139

End: 2050500

Strand: Reverse

Name: cho [H]

Synonym: Daro_1907

Alternate gene names: 71907536

Gene position: 2050500-2049139 (Counterclockwise)

Preceding gene: 71907539

Following gene: 71907535

Centisome position: 45.56

GC content: 61.75

Gene sequence:

>1362_bases
ATGCGTCCATTAGCCTTCGTCGACCTCGAAACCACCGGCGCCACCGCCACCCATGACCGCATCACCGAAATCGGCATTGT
CGAAGTTGATGCCGATGGCTCGGTGCGCGAATGGCAGCAGCTGGTCAATCCCGGCATGCGGATTCCACCTTTCATCGAGC
AGCTGACCGGCATCAGCGACGAAATGGTGGCCGATGCCCCCCCCTTCGAGGCAATCGCCGGCGAGGTCATGAAGCGGCTG
GAGGGGCGGCTGTTCATCGCCCATAACGCACGCTTCGACTATGGCTTCCTGAAGAATGAATTCAAGCGGCTCGGCGTCAC
CTTCCGCGCCGCCGTGTTGTGCACCGTCAAGCTGTCACGCACCCTGTTTCCCGAACACAAGCGGCACAACCTCGACAGTT
TGATCGAGCGCCACGAACTGCGCGCCGAGGCCCGTCACCGGGCGCTGGCCGACGCCCAGCTGATCCACCAGTTCTGGCAA
AAGATCCACCTCGAGCGCAGCCTTGAAGCCATCGATGCCGCCCTCAAGGCCCAGAATACCCACCCTAGCCTGCCGCCGCA
GCTTGATGCCGGCATTCTTGAAGATCTGCCCGAGTCGCCAGGTGTTTACCTGTTCTACGGCGACAACGACCTGCCGATTT
ATATCGGCAAGGCCAAGGATATCCGCCAGCGCGTGCTGTCGCACTTCTCCGGCGACCATAGCTCCGCCAAGGAAATGGCG
CTCTCCCAGCAGCTCAGGCGTATCGACTGGATCGAAACAGCGGGCGAGATCGGTGCCCTGCTCAAGGAGTCGAGGCTGGT
CAAGACATTAATGCCGACGGCCAATCGGCAACTGCGCAAGAACGATGATGTCTGCACCTGGACGCTGGTCGATGAAGGTG
AAGGCTGGCTGCGCCCCCAGCTGGCCCTGGCCGCCGATCTTGATTTCGACATTCACACCGCCTGCTACGGGCTGTTCAAG
AGCACCAGGGAGGCCAACGATGTGCTGCGAGCGCTGGTCGAATCACACCATCTGTGTGATGTCCTGACCGGCCTGGATAA
GGCGAAGCCCGGCAAACCCTGCTTCGGCTACCAGATCAAGCGCTGCAAGGGTGCCTGCATCGGCAAGGAGCCGCTGGCCA
AGCACACGATGCGCCTGATGACCGCGCTGACCAGCATGAAGCTGGTTTCCTGGCCTTTCACCGGCCCGGCAGTGATCCGC
GAAGGCGAGGAAGCCCATCTGATCAACGGCTGGCGCTACCTCGGCACGGCCAAAAGCGACGAAGAACTCCACGCCCTGCT
CGACGGCTCGCGTCCGCCATTCGACCGCGACGCTTACAAGCTACTGGCCAAATACGTCGGCAAGATGGTGCCGTTGCGCT
GA

Upstream 100 bases:

>100_bases
AAACACTGGATACCGCCCTCAATAGACCGCCATACACTAGCGGTAGCCCATTCGTGGCAAAAGAAGCGTTGGAACAAGCC
GGCCGTGAGAAAATTCGCCC

Downstream 100 bases:

>100_bases
ACACGTCAATAAGCGGGGGCGTGACATCGTCATCAAGCAGCACGAATCGTGGCAAACTGAAGGAATGCCATTCGCCCGGA
ATCCCGTCATGACATCGCAC

Product: DNA polymerase III, epsilon subunit

Products: diphosphate; DNAn+1

Alternate protein names: Endonuclease cho; UvrC homolog protein [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MRPLAFVDLETTGATATHDRITEIGIVEVDADGSVREWQQLVNPGMRIPPFIEQLTGISDEMVADAPPFEAIAGEVMKRL
EGRLFIAHNARFDYGFLKNEFKRLGVTFRAAVLCTVKLSRTLFPEHKRHNLDSLIERHELRAEARHRALADAQLIHQFWQ
KIHLERSLEAIDAALKAQNTHPSLPPQLDAGILEDLPESPGVYLFYGDNDLPIYIGKAKDIRQRVLSHFSGDHSSAKEMA
LSQQLRRIDWIETAGEIGALLKESRLVKTLMPTANRQLRKNDDVCTWTLVDEGEGWLRPQLALAADLDFDIHTACYGLFK
STREANDVLRALVESHHLCDVLTGLDKAKPGKPCFGYQIKRCKGACIGKEPLAKHTMRLMTALTSMKLVSWPFTGPAVIR
EGEEAHLINGWRYLGTAKSDEELHALLDGSRPPFDRDAYKLLAKYVGKMVPLR

Sequences:

>Translated_453_residues
MRPLAFVDLETTGATATHDRITEIGIVEVDADGSVREWQQLVNPGMRIPPFIEQLTGISDEMVADAPPFEAIAGEVMKRL
EGRLFIAHNARFDYGFLKNEFKRLGVTFRAAVLCTVKLSRTLFPEHKRHNLDSLIERHELRAEARHRALADAQLIHQFWQ
KIHLERSLEAIDAALKAQNTHPSLPPQLDAGILEDLPESPGVYLFYGDNDLPIYIGKAKDIRQRVLSHFSGDHSSAKEMA
LSQQLRRIDWIETAGEIGALLKESRLVKTLMPTANRQLRKNDDVCTWTLVDEGEGWLRPQLALAADLDFDIHTACYGLFK
STREANDVLRALVESHHLCDVLTGLDKAKPGKPCFGYQIKRCKGACIGKEPLAKHTMRLMTALTSMKLVSWPFTGPAVIR
EGEEAHLINGWRYLGTAKSDEELHALLDGSRPPFDRDAYKLLAKYVGKMVPLR
>Mature_453_residues
MRPLAFVDLETTGATATHDRITEIGIVEVDADGSVREWQQLVNPGMRIPPFIEQLTGISDEMVADAPPFEAIAGEVMKRL
EGRLFIAHNARFDYGFLKNEFKRLGVTFRAAVLCTVKLSRTLFPEHKRHNLDSLIERHELRAEARHRALADAQLIHQFWQ
KIHLERSLEAIDAALKAQNTHPSLPPQLDAGILEDLPESPGVYLFYGDNDLPIYIGKAKDIRQRVLSHFSGDHSSAKEMA
LSQQLRRIDWIETAGEIGALLKESRLVKTLMPTANRQLRKNDDVCTWTLVDEGEGWLRPQLALAADLDFDIHTACYGLFK
STREANDVLRALVESHHLCDVLTGLDKAKPGKPCFGYQIKRCKGACIGKEPLAKHTMRLMTALTSMKLVSWPFTGPAVIR
EGEEAHLINGWRYLGTAKSDEELHALLDGSRPPFDRDAYKLLAKYVGKMVPLR

Specific function: Incises the DNA at the 3' side of a lesion during nucleotide excision repair. Incises the DNA farther away from the lesion than uvrC. Not able to incise the 5' site of a lesion. When a lesion remains because uvrC is not able to induce the 3' incision, cho

COG id: COG0322

COG function: function code L; Nuclease subunit of the excinuclease complex

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrC C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788037, Length=266, Percent_Identity=40.2255639097744, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1786409, Length=166, Percent_Identity=33.1325301204819, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081999, Length=185, Percent_Identity=29.7297297297297, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000305 [H]

Pfam domain/function: PF01541 GIY-YIG [H]

EC number: 2.7.7.7

Molecular weight: Translated: 50950; Mature: 50950

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS50164 UVRC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPLAFVDLETTGATATHDRITEIGIVEVDADGSVREWQQLVNPGMRIPPFIEQLTGISD
CCCEEEEEEECCCCCCCHHHHHEEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHCCCCH
EMVADAPPFEAIAGEVMKRLEGRLFIAHNARFDYGFLKNEFKRLGVTFRAAVLCTVKLSR
HHHCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHH
TLFPEHKRHNLDSLIERHELRAEARHRALADAQLIHQFWQKIHLERSLEAIDAALKAQNT
HHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HPSLPPQLDAGILEDLPESPGVYLFYGDNDLPIYIGKAKDIRQRVLSHFSGDHSSAKEMA
CCCCCCCCCCHHHHHCCCCCCEEEEECCCCCEEEECCHHHHHHHHHHHHCCCCHHHHHHH
LSQQLRRIDWIETAGEIGALLKESRLVKTLMPTANRQLRKNDDVCTWTLVDEGEGWLRPQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEEEECCCCCCCCC
LALAADLDFDIHTACYGLFKSTREANDVLRALVESHHLCDVLTGLDKAKPGKPCFGYQIK
CCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
RCKGACIGKEPLAKHTMRLMTALTSMKLVSWPFTGPAVIREGEEAHLINGWRYLGTAKSD
HHCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCHHHHCCCCCCEEECCEEEECCCCCH
EELHALLDGSRPPFDRDAYKLLAKYVGKMVPLR
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRPLAFVDLETTGATATHDRITEIGIVEVDADGSVREWQQLVNPGMRIPPFIEQLTGISD
CCCEEEEEEECCCCCCCHHHHHEEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHCCCCH
EMVADAPPFEAIAGEVMKRLEGRLFIAHNARFDYGFLKNEFKRLGVTFRAAVLCTVKLSR
HHHCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHH
TLFPEHKRHNLDSLIERHELRAEARHRALADAQLIHQFWQKIHLERSLEAIDAALKAQNT
HHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HPSLPPQLDAGILEDLPESPGVYLFYGDNDLPIYIGKAKDIRQRVLSHFSGDHSSAKEMA
CCCCCCCCCCHHHHHCCCCCCEEEEECCCCCEEEECCHHHHHHHHHHHHCCCCHHHHHHH
LSQQLRRIDWIETAGEIGALLKESRLVKTLMPTANRQLRKNDDVCTWTLVDEGEGWLRPQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEEEECCCCCCCCC
LALAADLDFDIHTACYGLFKSTREANDVLRALVESHHLCDVLTGLDKAKPGKPCFGYQIK
CCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
RCKGACIGKEPLAKHTMRLMTALTSMKLVSWPFTGPAVIREGEEAHLINGWRYLGTAKSD
HHCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCHHHHCCCCCCEEECCEEEECCCCCH
EELHALLDGSRPPFDRDAYKLLAKYVGKMVPLR
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxynucleoside triphosphate; DNAn

Specific reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]