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The map label for this gene is trmA

Identifier: 71907452

GI number: 71907452

Start: 1960388

End: 1961476

Strand: Reverse

Name: trmA

Synonym: Daro_1823

Alternate gene names: 71907452

Gene position: 1961476-1960388 (Counterclockwise)

Preceding gene: 71907453

Following gene: 71907451

Centisome position: 43.58

GC content: 62.08

Gene sequence:

>1089_bases
ATGCCGCTCCCGACCTACGATCCCGCCGACTACCCGACCCAGCTTGCGACCAAGGTCGCCCATTTCGAGCAAAACTTCGC
TCCCTTCGGCGTTGCAAATACGGCCATACATGCCTCCGCGCCGCTGCATTACCGGATGCGCGCCGAGTTCCGCATCTGGC
ACGAGGGCGATGATCTCAACTACGCGATGTTCGATCCGGCCGATCCCAAACAACCGATCACCCTCGAAACCTTCCCGCCG
GCTGCCGAATCGATTTGCCACCTGATGCCGCGCCTGCGCGACAAGCTCCGGGGCAACGAGAGCCTGCGCCGCCGGCTGTT
CCAGGCCGATTTCCTGGCCACGCTGAGCGGCGAAATGCTGGTCACCTTGATCTACCACCGCCAACTCGACGAGGCTTGGG
AAGCTGCGGCCCGTGAAATGGCGGCCGAACTCGGCATCGGCCTGATCGGCCGCAGCCGGGGTCAGAAAATCGTCCTCGAC
CGCGACTGGGTGCTCGAAGGCTTCGAACTGAACGGCCGGCAGCTCCGTTACAAGCAGGTCGAAGGCAGCTTCACCCAGCC
CAACGGCGGGGTCAATCGCCAGATGCTGGGCTGGGCCTGCCAGCAAGCCGCCGGGTTTGGCGGCAACCTGGTGGAGCTTT
ACTGCGGCAACGGCAATTTCACTATCGCGCTGTCGCCGCTGTTCGAGCGCGTACTGGCCACCGAAGTCAGCAAATCGTCG
GTCCATGCCGCCCAGTACAACCTGGCCGCCAATCAGGTCGAAAACGTCGCGCTGGTTCGCATGTCGAGCGAAGAATTCAG
TAATGCCCTGGCTGGCCGCGAAGAATTCCAGCGGCTGAAGGATATCGATCTCGAGCCCTTCCGCCACGCCACCTTGTTCG
TCGATCCGCCACGCAGTGGGCTGGATGCCGTCACACTCGAACTCGCCCGTGGTTTCGACCGTATCCTGTACATTTCCTGC
AACCAGGAAACCCTGCGGGAGAATGTCATCGCGCTGCAGGACACCCACGAAATCAAAGCTTCAGCCGTGTTCGACCAGTT
CCCCTACACACACCATCTGGAATGCGGGCTGTTGCTGACCCGGCGATAG

Upstream 100 bases:

>100_bases
CGGATGCGCTGACCGCTCGCCCCACCCGGCAGACCGGCGTATATTTCGCCCCTTGCCGCGACCGCCACCCGGCGCCCTAA
CCTGCTCCTGCTCCAAGACC

Downstream 100 bases:

>100_bases
CAGTACGATCCAGCATCCCTTTCCAGCGCTGACTGATTGACTTAAGCAGAACAGACCATGATGCCCGCTCCGCCACAGCC
CATCGACCGGGATGTTCCCT

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: tRNA(M-5-U54)-methyltransferase; RUMT

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MPLPTYDPADYPTQLATKVAHFEQNFAPFGVANTAIHASAPLHYRMRAEFRIWHEGDDLNYAMFDPADPKQPITLETFPP
AAESICHLMPRLRDKLRGNESLRRRLFQADFLATLSGEMLVTLIYHRQLDEAWEAAAREMAAELGIGLIGRSRGQKIVLD
RDWVLEGFELNGRQLRYKQVEGSFTQPNGGVNRQMLGWACQQAAGFGGNLVELYCGNGNFTIALSPLFERVLATEVSKSS
VHAAQYNLAANQVENVALVRMSSEEFSNALAGREEFQRLKDIDLEPFRHATLFVDPPRSGLDAVTLELARGFDRILYISC
NQETLRENVIALQDTHEIKASAVFDQFPYTHHLECGLLLTRR

Sequences:

>Translated_362_residues
MPLPTYDPADYPTQLATKVAHFEQNFAPFGVANTAIHASAPLHYRMRAEFRIWHEGDDLNYAMFDPADPKQPITLETFPP
AAESICHLMPRLRDKLRGNESLRRRLFQADFLATLSGEMLVTLIYHRQLDEAWEAAAREMAAELGIGLIGRSRGQKIVLD
RDWVLEGFELNGRQLRYKQVEGSFTQPNGGVNRQMLGWACQQAAGFGGNLVELYCGNGNFTIALSPLFERVLATEVSKSS
VHAAQYNLAANQVENVALVRMSSEEFSNALAGREEFQRLKDIDLEPFRHATLFVDPPRSGLDAVTLELARGFDRILYISC
NQETLRENVIALQDTHEIKASAVFDQFPYTHHLECGLLLTRR
>Mature_361_residues
PLPTYDPADYPTQLATKVAHFEQNFAPFGVANTAIHASAPLHYRMRAEFRIWHEGDDLNYAMFDPADPKQPITLETFPPA
AESICHLMPRLRDKLRGNESLRRRLFQADFLATLSGEMLVTLIYHRQLDEAWEAAAREMAAELGIGLIGRSRGQKIVLDR
DWVLEGFELNGRQLRYKQVEGSFTQPNGGVNRQMLGWACQQAAGFGGNLVELYCGNGNFTIALSPLFERVLATEVSKSSV
HAAQYNLAANQVENVALVRMSSEEFSNALAGREEFQRLKDIDLEPFRHATLFVDPPRSGLDAVTLELARGFDRILYISCN
QETLRENVIALQDTHEIKASAVFDQFPYTHHLECGLLLTRR

Specific function: Catalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily

Homologues:

Organism=Escherichia coli, GI1790403, Length=365, Percent_Identity=49.041095890411, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI1789148, Length=338, Percent_Identity=25.1479289940828, Blast_Score=77, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24666579, Length=166, Percent_Identity=27.1084337349398, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRMA_DECAR (Q47F12)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_285039.1
- ProteinModelPortal:   Q47F12
- SMR:   Q47F12
- STRING:   Q47F12
- GeneID:   3566077
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_1823
- NMPDR:   fig|159087.4.peg.1834
- eggNOG:   COG2265
- HOGENOM:   HBG608402
- OMA:   NEVRPFR
- ProtClustDB:   PRK05031
- BioCyc:   DARO159087:DARO_1823-MONOMER
- HAMAP:   MF_01011
- InterPro:   IPR011869
- InterPro:   IPR010280
- TIGRFAMs:   TIGR02143

Pfam domain/function: PF05958 tRNA_U5-meth_tr

EC number: =2.1.1.35

Molecular weight: Translated: 40809; Mature: 40678

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 320-320 BINDING 186-186 BINDING 214-214 BINDING 219-219 BINDING 235-235 BINDING 295-295

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLPTYDPADYPTQLATKVAHFEQNFAPFGVANTAIHASAPLHYRMRAEFRIWHEGDDLN
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCCEEEEEEEEEEEEECCCCCC
YAMFDPADPKQPITLETFPPAAESICHLMPRLRDKLRGNESLRRRLFQADFLATLSGEML
EEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHH
VTLIYHRQLDEAWEAAAREMAAELGIGLIGRSRGQKIVLDRDWVLEGFELNGRQLRYKQV
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEECCCEEECEECCCCEEEEEEC
EGSFTQPNGGVNRQMLGWACQQAAGFGGNLVELYCGNGNFTIALSPLFERVLATEVSKSS
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEHHHHHHHHHHHHHHHH
VHAAQYNLAANQVENVALVRMSSEEFSNALAGREEFQRLKDIDLEPFRHATLFVDPPRSG
HHHHHHHHHHHHHCCEEEEEECHHHHHHHHCCHHHHHHHHCCCCCCCCCEEEEECCCCCC
LDAVTLELARGFDRILYISCNQETLRENVIALQDTHEIKASAVFDQFPYTHHLECGLLLT
HHHHHHHHHCCCCEEEEEECCHHHHHHCCEEEECCHHHHHHHHHHHCCCCEECEECEEEE
RR
CC
>Mature Secondary Structure 
PLPTYDPADYPTQLATKVAHFEQNFAPFGVANTAIHASAPLHYRMRAEFRIWHEGDDLN
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCCEEEEEEEEEEEEECCCCCC
YAMFDPADPKQPITLETFPPAAESICHLMPRLRDKLRGNESLRRRLFQADFLATLSGEML
EEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHH
VTLIYHRQLDEAWEAAAREMAAELGIGLIGRSRGQKIVLDRDWVLEGFELNGRQLRYKQV
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEECCCEEECEECCCCEEEEEEC
EGSFTQPNGGVNRQMLGWACQQAAGFGGNLVELYCGNGNFTIALSPLFERVLATEVSKSS
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEHHHHHHHHHHHHHHHH
VHAAQYNLAANQVENVALVRMSSEEFSNALAGREEFQRLKDIDLEPFRHATLFVDPPRSG
HHHHHHHHHHHHHCCEEEEEECHHHHHHHHCCHHHHHHHHCCCCCCCCCEEEEECCCCCC
LDAVTLELARGFDRILYISCNQETLRENVIALQDTHEIKASAVFDQFPYTHHLECGLLLT
HHHHHHHHHCCCCEEEEEECCHHHHHHCCEEEECCHHHHHHHHHHHCCCCEECEECEEEE
RR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA