Click here to switch to the map view.

The map label for this gene is glcA [H]

Identifier: 71907392

GI number: 71907392

Start: 1893217

End: 1894953

Strand: Reverse

Name: glcA [H]

Synonym: Daro_1762

Alternate gene names: 71907392

Gene position: 1894953-1893217 (Counterclockwise)

Preceding gene: 71907393

Following gene: 71907391

Centisome position: 42.1

GC content: 61.72

Gene sequence:

>1737_bases
ATGGTCTGGCAACAGATATACGATCCATTGGGCAGCATTGGGCTGTCCGCATTACTGGCGTCCGTTCCGGTCGTCGTCAT
GCTGGCCGCATTGGCCTTCTTCCACGTCAAGGCACACGTTGCGGCGCTGCTGGCCTTGATCTCAGCCCTTGGCATTGCCA
TTTTCGGTTTTGGCATGCCGGCCAACATGGCCACCGATGCGGCATTGTTCGGTGCCATCAACGGTCTGGTGCCGATCGGC
TGGATCGTGCTCAACATCATCTTCCTGCACCGCCTGACGACCGAAAACGGCTCGTTCAAGGTGCTGCAGGACTCCATCGC
CGGCATCACCGATGACCGTCGCCTGCAACTGCTGCTGATCGCCTTCTGCTTCGGTGCCTTCTTTGAAGGTGCTGCCGGCT
TCGGTACGCCGGTCGCTGTCACCGGTGCCGTGCTGATCGGTCTCGGTTTCTCGCCGCTGGCCGCTTCCGGCCTGGCCCTG
ATCGCCAACACTGCCCCGGTTGCCTACGGGGCGCTGGGTACCCCGGTCATCACGCTGGCCAAGGTCACCGGCCTTGACGA
GATGATGCTGTCGTCGATGATTGGTCGCCAGCTGCCGTTCTTCTCCCTGCTCGTGCCGTTCTGGCTGATCTGGGCCTTTG
CCGGGCGCAAGGGCATGATGCAGATCTGGCCGGCCATCCTGGTGGCCGGCGGTTCCTTTGCCATCGCCCAGTTCGTCGTA
TCCAACTACCACGGCCCGATGCTGGTCGATGTCATCGCCTCGCTGGTGTCCATGGCCAGCCTGATCGCCTTCCTGAAGGT
CTGGAAACCGAAGAAGGTGTGGACCTCCGCCAAGCTGATCGGCCACGAGGAAGATGGTGGCGAAGCCCATCCGGAGCCGA
CCGACCTCCCACAAAAGGCTGACAAGGTCGCCGTCCGTCGCGCCTGGATGCCGTGGGTTGTGCTGACTGTCTTCGTCTTC
ATCTGGGGCCTGCCCGAGTTCAAGAAGGCTGTCGATACCGCGCCGGTCCTCGAAAACGGCCAGCCGGTGCTCGACGCCAA
GGGCCTGCCGAAACTGGCCGGCAACACCTTCTTTGCCCCGGTTTTCAAGTTCGAGGATATCCACGAGAAAATCGAGAAGG
TGCCGCCCGTCGTTGCCAAGAGCAAGAAGGAAGAAGCTGCCTACAAGCTGAACTGGTTCACCACCACCGGCACCGGCATC
CTGCTTGCGGCCCTGTTCGCCGGCCTGATGATGGGCTACCGCCCGCTGCAACTGGTCAGCCAGTTCGGCAAGACGGTCTG
GATGGTGCGTTATTCACTGACCACCATTGTTGCCATGCTGGCCCTCGGCTACCTGACGCGCTTTGCCGGCATCGATGCCA
CCCTCGGCCTGGCCTTCGCCTTCACCGGCGTGTTCTATCCCTTCTTCGGCACCTTCCTCGGCTGGCTGGGTGTCGCGCTG
ACCGGTTCAGACACGGCTTCCAACGTGCTGTTCGGCGGCCTGCAACGGACCACCGCTGAACAGCTTGGCCTCTCGCCGAT
TCTGATGGCGGCTGCCAACAGCTCGGGTGGCGTGATGGGCAAGATGATCGACGCGCAATCGATCGTCGTCGCCTCCACCG
CGACCCACTGGTATGGCCATGAAGGCGAGATCCTGCGTTACGTCTTCTTCCATTCCATCGCGCTGTGCACCCTGATGGGC
TTCTTCATCACGGCACAAGCCTATGTCTGGCCCTTTACGGCCATGGTCCTCAAATAA

Upstream 100 bases:

>100_bases
GGGCCAAAAACTGAGACAAATCTGACACTAAAATGGTCACGCCACCCGTCTGCGCGCCAACGCAGCAAGTGGTCTGACCA
GATGATAAAGGAATTCATCC

Downstream 100 bases:

>100_bases
TCGGAGCCGCACACATGTATCAGAAAATCTTTGTCGCCATCGACGACAGCCCGACCTCGCAGAAGGCATTGGCCGAAGCC
ATCAATCTGGCCTGGATGTC

Product: L-lactate permease

Products: lactate [Cytoplasm]; Proton [Cytoplasm]; glycolate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 578; Mature: 578

Protein sequence:

>578_residues
MVWQQIYDPLGSIGLSALLASVPVVVMLAALAFFHVKAHVAALLALISALGIAIFGFGMPANMATDAALFGAINGLVPIG
WIVLNIIFLHRLTTENGSFKVLQDSIAGITDDRRLQLLLIAFCFGAFFEGAAGFGTPVAVTGAVLIGLGFSPLAASGLAL
IANTAPVAYGALGTPVITLAKVTGLDEMMLSSMIGRQLPFFSLLVPFWLIWAFAGRKGMMQIWPAILVAGGSFAIAQFVV
SNYHGPMLVDVIASLVSMASLIAFLKVWKPKKVWTSAKLIGHEEDGGEAHPEPTDLPQKADKVAVRRAWMPWVVLTVFVF
IWGLPEFKKAVDTAPVLENGQPVLDAKGLPKLAGNTFFAPVFKFEDIHEKIEKVPPVVAKSKKEEAAYKLNWFTTTGTGI
LLAALFAGLMMGYRPLQLVSQFGKTVWMVRYSLTTIVAMLALGYLTRFAGIDATLGLAFAFTGVFYPFFGTFLGWLGVAL
TGSDTASNVLFGGLQRTTAEQLGLSPILMAAANSSGGVMGKMIDAQSIVVASTATHWYGHEGEILRYVFFHSIALCTLMG
FFITAQAYVWPFTAMVLK

Sequences:

>Translated_578_residues
MVWQQIYDPLGSIGLSALLASVPVVVMLAALAFFHVKAHVAALLALISALGIAIFGFGMPANMATDAALFGAINGLVPIG
WIVLNIIFLHRLTTENGSFKVLQDSIAGITDDRRLQLLLIAFCFGAFFEGAAGFGTPVAVTGAVLIGLGFSPLAASGLAL
IANTAPVAYGALGTPVITLAKVTGLDEMMLSSMIGRQLPFFSLLVPFWLIWAFAGRKGMMQIWPAILVAGGSFAIAQFVV
SNYHGPMLVDVIASLVSMASLIAFLKVWKPKKVWTSAKLIGHEEDGGEAHPEPTDLPQKADKVAVRRAWMPWVVLTVFVF
IWGLPEFKKAVDTAPVLENGQPVLDAKGLPKLAGNTFFAPVFKFEDIHEKIEKVPPVVAKSKKEEAAYKLNWFTTTGTGI
LLAALFAGLMMGYRPLQLVSQFGKTVWMVRYSLTTIVAMLALGYLTRFAGIDATLGLAFAFTGVFYPFFGTFLGWLGVAL
TGSDTASNVLFGGLQRTTAEQLGLSPILMAAANSSGGVMGKMIDAQSIVVASTATHWYGHEGEILRYVFFHSIALCTLMG
FFITAQAYVWPFTAMVLK
>Mature_578_residues
MVWQQIYDPLGSIGLSALLASVPVVVMLAALAFFHVKAHVAALLALISALGIAIFGFGMPANMATDAALFGAINGLVPIG
WIVLNIIFLHRLTTENGSFKVLQDSIAGITDDRRLQLLLIAFCFGAFFEGAAGFGTPVAVTGAVLIGLGFSPLAASGLAL
IANTAPVAYGALGTPVITLAKVTGLDEMMLSSMIGRQLPFFSLLVPFWLIWAFAGRKGMMQIWPAILVAGGSFAIAQFVV
SNYHGPMLVDVIASLVSMASLIAFLKVWKPKKVWTSAKLIGHEEDGGEAHPEPTDLPQKADKVAVRRAWMPWVVLTVFVF
IWGLPEFKKAVDTAPVLENGQPVLDAKGLPKLAGNTFFAPVFKFEDIHEKIEKVPPVVAKSKKEEAAYKLNWFTTTGTGI
LLAALFAGLMMGYRPLQLVSQFGKTVWMVRYSLTTIVAMLALGYLTRFAGIDATLGLAFAFTGVFYPFFGTFLGWLGVAL
TGSDTASNVLFGGLQRTTAEQLGLSPILMAAANSSGGVMGKMIDAQSIVVASTATHWYGHEGEILRYVFFHSIALCTLMG
FFITAQAYVWPFTAMVLK

Specific function: Transports glycolate across the membrane. Can also transport L-lactate and D-lactate. Seems to be driven by a proton motive force [H]

COG id: COG1620

COG function: function code C; L-lactate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lactate permease family [H]

Homologues:

Organism=Escherichia coli, GI1789347, Length=583, Percent_Identity=44.9399656946827, Blast_Score=470, Evalue=1e-133,
Organism=Escherichia coli, GI1790031, Length=568, Percent_Identity=41.9014084507042, Blast_Score=424, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003804 [H]

Pfam domain/function: PF02652 Lactate_perm [H]

EC number: NA

Molecular weight: Translated: 61946; Mature: 61946

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVWQQIYDPLGSIGLSALLASVPVVVMLAALAFFHVKAHVAALLALISALGIAIFGFGMP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ANMATDAALFGAINGLVPIGWIVLNIIFLHRLTTENGSFKVLQDSIAGITDDRRLQLLLI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHCCCCCCHHHHHHHH
AFCFGAFFEGAAGFGTPVAVTGAVLIGLGFSPLAASGLALIANTAPVAYGALGTPVITLA
HHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHCCHHEEECCCCHHHCCCCCHHHHHH
KVTGLDEMMLSSMIGRQLPFFSLLVPFWLIWAFAGRKGMMQIWPAILVAGGSFAIAQFVV
HHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHH
SNYHGPMLVDVIASLVSMASLIAFLKVWKPKKVWTSAKLIGHEEDGGEAHPEPTDLPQKA
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHH
DKVAVRRAWMPWVVLTVFVFIWGLPEFKKAVDTAPVLENGQPVLDAKGLPKLAGNTFFAP
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHCCCCHHHH
VFKFEDIHEKIEKVPPVVAKSKKEEAAYKLNWFTTTGTGILLAALFAGLMMGYRPLQLVS
HHHHHHHHHHHHHCCCHHHCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHCCHHHHHHH
QFGKTVWMVRYSLTTIVAMLALGYLTRFAGIDATLGLAFAFTGVFYPFFGTFLGWLGVAL
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEE
TGSDTASNVLFGGLQRTTAEQLGLSPILMAAANSSGGVMGKMIDAQSIVVASTATHWYGH
CCCCHHHHHHHCCHHHHHHHHHCCCHHHHEECCCCCCCHHHHCCCCCEEEEECHHHCCCC
EGEILRYVFFHSIALCTLMGFFITAQAYVWPFTAMVLK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVWQQIYDPLGSIGLSALLASVPVVVMLAALAFFHVKAHVAALLALISALGIAIFGFGMP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ANMATDAALFGAINGLVPIGWIVLNIIFLHRLTTENGSFKVLQDSIAGITDDRRLQLLLI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHCCCCCCHHHHHHHH
AFCFGAFFEGAAGFGTPVAVTGAVLIGLGFSPLAASGLALIANTAPVAYGALGTPVITLA
HHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHCCHHEEECCCCHHHCCCCCHHHHHH
KVTGLDEMMLSSMIGRQLPFFSLLVPFWLIWAFAGRKGMMQIWPAILVAGGSFAIAQFVV
HHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHH
SNYHGPMLVDVIASLVSMASLIAFLKVWKPKKVWTSAKLIGHEEDGGEAHPEPTDLPQKA
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHH
DKVAVRRAWMPWVVLTVFVFIWGLPEFKKAVDTAPVLENGQPVLDAKGLPKLAGNTFFAP
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHCCCCHHHH
VFKFEDIHEKIEKVPPVVAKSKKEEAAYKLNWFTTTGTGILLAALFAGLMMGYRPLQLVS
HHHHHHHHHHHHHCCCHHHCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHCCHHHHHHH
QFGKTVWMVRYSLTTIVAMLALGYLTRFAGIDATLGLAFAFTGVFYPFFGTFLGWLGVAL
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEE
TGSDTASNVLFGGLQRTTAEQLGLSPILMAAANSSGGVMGKMIDAQSIVVASTATHWYGH
CCCCHHHHHHHCCHHHHHHHHHCCCHHHHEECCCCCCCHHHHCCCCCEEEEECHHHCCCC
EGEILRYVFFHSIALCTLMGFFITAQAYVWPFTAMVLK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactate [Periplasm]; Proton [Periplasm]; glycolate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactate [Periplasm] = Proton [Cytoplasm] + lactate [Cytoplasm] Proton [Periplasm] + glycolate [Periplasm] = Proton [Cytoplasm] + glycolate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 11283302; 11785976 [H]