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The map label for this gene is dapE

Identifier: 71907354

GI number: 71907354

Start: 1852193

End: 1853326

Strand: Reverse

Name: dapE

Synonym: Daro_1724

Alternate gene names: 71907354

Gene position: 1853326-1852193 (Counterclockwise)

Preceding gene: 71907355

Following gene: 71907353

Centisome position: 41.17

GC content: 62.17

Gene sequence:

>1134_bases
ATGTCCGACACCACGCTCGAACTGGCCAAGCGAATCATCGCCTGCGCCTCCGTGACGCCTGAAGACGCAGGCTGCATGGA
CATTCTGATCGAACGGCTGAAGCCGCTCGGCTTCAGCATCGAATTCATCAATCGCAACGGCGTGACCAACCTGTGGGCCC
GCCGCGGCACGACCGCACCGCTGTTCGTCTTCGCTGGCCATACCGACGTCGTGCCGACCGGCCCGCTCGACAAATGGACC
TCGCCCCCCTTCGCCCCGGAAATCCGCGACGGCGTGCTGTATGGGCGTGGCACGGCCGACATGAAATCCTCGGTCGCCGC
TTCGGTAACCGCCGTCGAAGCCTTTGTTGCGGCCAACCCACAACACCCCGGCTCCCTGGCTTTCCTGCTTACCTCCGATG
AAGAAGGTGACGCCAATGACGGCACCATCGCTGTCGTTGAGGCGCTGAAAGCCCGTGGCGAAACGCTCGATTTCTGCATC
ATCGGCGAACCGACCTCGGTCGATACGCTCGGCGACATGGTCAAGAACGGCCGCCGCGGATCGCTGTCCGGCGTGCTGAC
GGTCAAGGGCATCCAGTGCCACATCGCCTATCCGGAAAAGGGGCGCAATCCGATCCACGAAGCCGCTCCGGCGCTGGCCG
AACTGGCTGCGACCGAATGGGATCAGGGCAACGAGTATTACCAGCCGACTACCTGGCAAATTTCCAACATTCACGGCGGC
ACCGGCGCCACTAACGTTGTACCGGGCAGCGTCGACATCAAGTTCAATTTCCGTTTCTCGACGGCCAGCACGCCGGAAGG
GCTGCAACAACGCCTGTCCGCCATCCTGGAAAAGCACAAGCTCGACTACGAAATCAAATGGACGCTGGGCGCCCGCCCCT
TCCTGACCGGCCGTGGCCCACTGGCCGATGCGGCGACGACGGCCATCCGCGAAATCTGCGGCATCGAAACCGAACTATCG
ACCACCGGCGGCACCTCCGACGGCCGTTTCATTGCCGAAATCTGCCGCCAGATGCTCGAAATCGGCCCGGTCAATGCCAC
CAGCCACAAGATCGACGAATGCATTGCCGTCGACGCATTGCCCAAGCTGTCCGCCATCTATCGCCGCATCCTTGAACAAT
TGATGACCGCATGA

Upstream 100 bases:

>100_bases
AAACCGATGTCGCTCTCGAGTTCGACAGCACCAGCGATGGCGGCACTTCCTTCAAGGAATTCACGCTCAGCCTGGCTGAC
CACGACGAAGAGTCGCACTG

Downstream 100 bases:

>100_bases
GCGACCTTTCCGCCAAAAATGAACTGATCACCGTTCGCGACTATCTCCGCTACGCCGTCAGCCGCTTCAACGCGGCCCAG
CTTTTCTTCGGACACGGCAG

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MSDTTLELAKRIIACASVTPEDAGCMDILIERLKPLGFSIEFINRNGVTNLWARRGTTAPLFVFAGHTDVVPTGPLDKWT
SPPFAPEIRDGVLYGRGTADMKSSVAASVTAVEAFVAANPQHPGSLAFLLTSDEEGDANDGTIAVVEALKARGETLDFCI
IGEPTSVDTLGDMVKNGRRGSLSGVLTVKGIQCHIAYPEKGRNPIHEAAPALAELAATEWDQGNEYYQPTTWQISNIHGG
TGATNVVPGSVDIKFNFRFSTASTPEGLQQRLSAILEKHKLDYEIKWTLGARPFLTGRGPLADAATTAIREICGIETELS
TTGGTSDGRFIAEICRQMLEIGPVNATSHKIDECIAVDALPKLSAIYRRILEQLMTA

Sequences:

>Translated_377_residues
MSDTTLELAKRIIACASVTPEDAGCMDILIERLKPLGFSIEFINRNGVTNLWARRGTTAPLFVFAGHTDVVPTGPLDKWT
SPPFAPEIRDGVLYGRGTADMKSSVAASVTAVEAFVAANPQHPGSLAFLLTSDEEGDANDGTIAVVEALKARGETLDFCI
IGEPTSVDTLGDMVKNGRRGSLSGVLTVKGIQCHIAYPEKGRNPIHEAAPALAELAATEWDQGNEYYQPTTWQISNIHGG
TGATNVVPGSVDIKFNFRFSTASTPEGLQQRLSAILEKHKLDYEIKWTLGARPFLTGRGPLADAATTAIREICGIETELS
TTGGTSDGRFIAEICRQMLEIGPVNATSHKIDECIAVDALPKLSAIYRRILEQLMTA
>Mature_376_residues
SDTTLELAKRIIACASVTPEDAGCMDILIERLKPLGFSIEFINRNGVTNLWARRGTTAPLFVFAGHTDVVPTGPLDKWTS
PPFAPEIRDGVLYGRGTADMKSSVAASVTAVEAFVAANPQHPGSLAFLLTSDEEGDANDGTIAVVEALKARGETLDFCII
GEPTSVDTLGDMVKNGRRGSLSGVLTVKGIQCHIAYPEKGRNPIHEAAPALAELAATEWDQGNEYYQPTTWQISNIHGGT
GATNVVPGSVDIKFNFRFSTASTPEGLQQRLSAILEKHKLDYEIKWTLGARPFLTGRGPLADAATTAIREICGIETELST
TGGTSDGRFIAEICRQMLEIGPVNATSHKIDECIAVDALPKLSAIYRRILEQLMTA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=375, Percent_Identity=54.1333333333333, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI1790395, Length=270, Percent_Identity=28.5185185185185, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789236, Length=289, Percent_Identity=24.2214532871972, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17551016, Length=381, Percent_Identity=25.1968503937008, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_DECAR (Q47FB0)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_284941.1
- ProteinModelPortal:   Q47FB0
- SMR:   Q47FB0
- STRING:   Q47FB0
- MEROPS:   M20.010
- GeneID:   3568543
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_1724
- NMPDR:   fig|159087.4.peg.1737
- eggNOG:   COG0624
- HOGENOM:   HBG728841
- OMA:   PMVFEDT
- ProtClustDB:   PRK13009
- BioCyc:   DARO159087:DARO_1724-MONOMER
- GO:   GO:0006508
- HAMAP:   MF_01690
- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 40441; Mature: 40310

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 69-69 ACT_SITE 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTTLELAKRIIACASVTPEDAGCMDILIERLKPLGFSIEFINRNGVTNLWARRGTTAP
CCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCCE
LFVFAGHTDVVPTGPLDKWTSPPFAPEIRDGVLYGRGTADMKSSVAASVTAVEAFVAANP
EEEEECCCCCCCCCCCCCCCCCCCCCCHHCCEEECCCCHHHHHHHHHHHHHHHHHHHCCC
QHPGSLAFLLTSDEEGDANDGTIAVVEALKARGETLDFCIIGEPTSVDTLGDMVKNGRRG
CCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCCC
SLSGVLTVKGIQCHIAYPEKGRNPIHEAAPALAELAATEWDQGNEYYQPTTWQISNIHGG
CCCEEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCC
TGATNVVPGSVDIKFNFRFSTASTPEGLQQRLSAILEKHKLDYEIKWTLGARPFLTGRGP
CCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCEECCCCC
LADAATTAIREICGIETELSTTGGTSDGRFIAEICRQMLEIGPVNATSHKIDECIAVDAL
CHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
PKLSAIYRRILEQLMTA
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDTTLELAKRIIACASVTPEDAGCMDILIERLKPLGFSIEFINRNGVTNLWARRGTTAP
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCCE
LFVFAGHTDVVPTGPLDKWTSPPFAPEIRDGVLYGRGTADMKSSVAASVTAVEAFVAANP
EEEEECCCCCCCCCCCCCCCCCCCCCCHHCCEEECCCCHHHHHHHHHHHHHHHHHHHCCC
QHPGSLAFLLTSDEEGDANDGTIAVVEALKARGETLDFCIIGEPTSVDTLGDMVKNGRRG
CCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCCC
SLSGVLTVKGIQCHIAYPEKGRNPIHEAAPALAELAATEWDQGNEYYQPTTWQISNIHGG
CCCEEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCC
TGATNVVPGSVDIKFNFRFSTASTPEGLQQRLSAILEKHKLDYEIKWTLGARPFLTGRGP
CCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCEECCCCC
LADAATTAIREICGIETELSTTGGTSDGRFIAEICRQMLEIGPVNATSHKIDECIAVDAL
CHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
PKLSAIYRRILEQLMTA
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA