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The map label for this gene is ilvB [H]

Identifier: 71907251

GI number: 71907251

Start: 1737010

End: 1738680

Strand: Reverse

Name: ilvB [H]

Synonym: Daro_1620

Alternate gene names: 71907251

Gene position: 1738680-1737010 (Counterclockwise)

Preceding gene: 71907252

Following gene: 71907250

Centisome position: 38.63

GC content: 60.8

Gene sequence:

>1671_bases
ATGACTGAAACTCTCACCGGCGCGCAGATCACCGTTCGCCTGCTCGAACGCCAAGGCATTCGTACCGTTGCCGGTATTCC
CGGCGGCGCCATCCTGCCAATCTACGATGCCCTCGGCCAATCAAGCGCCATCCACCATGTACTGGCCCGCCACGAACAGG
GGGCCGGCTTCATGGCCCAGGGCATGGCCCGCTCTACCGGCCTGCCCGCTGTCTGCCTAGCGTCCTCCGGCCCAGGCGCG
ACCAACCTGCTGACGGCCATTGCCGATGCCAAGCTCGATTCGATCCCGTTGGTTGCCATCACCGGCCAAGTGCCGAAGGC
GATGATTGGTACCGATGCTTTTCAGGAGGTCGACACTTACGGACTCTCCATTCCGATCACCAAACACAACTTTCTCGTCT
CCTCCCCCGAGGAATTGCTGGCGGTGATTCCCCGCGCCTTCGAAATTGCCACCTCAGGCCGCCCCGGCCCGGTGCTGGTC
GACATCCCGAAAGATGTACAAACCCAGGCGATCGAAATTGGCGAATGGCCAGAGCCTGGTCGCGCCCAGCCAATCACTCC
GACAGACCCGGCGTTGATCGCGCAGGCTACAGCAATGATCAATGCTGCCGAACGCCCGATTCTTTATCTCGGTGGTGGCG
TTGTGCATTCCGGCGCAGCAAGGACGGCAGTCGATCTCGCCGAAAAATCCAATTTACCGACCGTCATGACCTTGATGGCA
CTCGGCACCATGCCGGTCGATCACCCGCTGTCGCTCGGCATGCTGGGCATGCACGCTGCCCGTTACACCAACCTCGCACT
TGAAGAATGCGACCTTCTGATCGCCGTCGGCGCCCGCTTCGATGACCGTGCCACCGGCAAGGTGGCCGCCTTTTGTCCCC
AGGCGAAAATTATCCATATCGATATCGACCCGGCTGAACTGGACAAGATCAAGACTGCTCATCTCGGCATCGCTGCTGAT
GTCCAGGAAGCGCTCACGCAGCTACTACCGGCCGTAGCTGACAACGACCGACAAGTCTGGGTGGATCGTAACAATACCTT
GAAGGCAGAATTTCCTTTCGAGATGCCGGGACTCGACAACCCGCGCTCTCACTTTGGCTTGATTCGCACCGTCGCCGCCT
GCCTGGACAATGAAGCCACTGTCACCACCGACGTCGGCCAGCACCAGATGTGGGTTGCCCAAGCCTACCCGCTGCGCCGG
CCACGCCAGTGGCTGACTTCTGGCGGGCTGGGCACCATGGGCTTTGGTGTACCAGCGGCCATCGGCGCCGCTCTGGCTGA
ACCGCAGCGCACCGTGGTCTGCTTCACCGGCGACGGTTCGATCCTGATGAACATCCAGGAACTGGTCACCGCCGCCGAGG
AAAACGTGAACGTGAAAATTGTGCTCATGGACAACGCAACGCTCGGTCTGGTGCACCAGCAGCAGACACTGTTCTACGGC
GAAAGACTGTTCGCCTCGCAATTCAAATCCTCGCCGGACTTCATCAAGATCGCACAAGGTTTCGGTATCGCAGCGGTCGA
CCTCGATCTGGCCGCCAACCCTTGTGCAGCCCTGATGGAAGCCATCGCTCGCCCCGGCCCCTGCCTAATCCACGCCAGTA
TCGATGCCGAGCAGAAGGTCTACCCCATGGTGCCACCGGGTGCCGCCAACCGTGACATGATTGGAGCCTGA

Upstream 100 bases:

>100_bases
AGCAGAAGCAGCACAGAACCCCAACCCCCGCCGGCGCAAACCGGCGGGGGTTTTGTTTTGGAGCCCCACCGGTTGAGCCA
CCAACCCAAAGGAAACCTCC

Downstream 100 bases:

>100_bases
CATGAACGCCATAACGAAAAATGAAAACGATGCCCTTGCCCGGGCAGTGCTGGAAATCAACGTCAATAACCACCCGGGCG
TGATGTCACACGTCGTCGGC

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS-I; Acetohydroxy-acid synthase I large subunit; ALS-I [H]

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MTETLTGAQITVRLLERQGIRTVAGIPGGAILPIYDALGQSSAIHHVLARHEQGAGFMAQGMARSTGLPAVCLASSGPGA
TNLLTAIADAKLDSIPLVAITGQVPKAMIGTDAFQEVDTYGLSIPITKHNFLVSSPEELLAVIPRAFEIATSGRPGPVLV
DIPKDVQTQAIEIGEWPEPGRAQPITPTDPALIAQATAMINAAERPILYLGGGVVHSGAARTAVDLAEKSNLPTVMTLMA
LGTMPVDHPLSLGMLGMHAARYTNLALEECDLLIAVGARFDDRATGKVAAFCPQAKIIHIDIDPAELDKIKTAHLGIAAD
VQEALTQLLPAVADNDRQVWVDRNNTLKAEFPFEMPGLDNPRSHFGLIRTVAACLDNEATVTTDVGQHQMWVAQAYPLRR
PRQWLTSGGLGTMGFGVPAAIGAALAEPQRTVVCFTGDGSILMNIQELVTAAEENVNVKIVLMDNATLGLVHQQQTLFYG
ERLFASQFKSSPDFIKIAQGFGIAAVDLDLAANPCAALMEAIARPGPCLIHASIDAEQKVYPMVPPGAANRDMIGA

Sequences:

>Translated_556_residues
MTETLTGAQITVRLLERQGIRTVAGIPGGAILPIYDALGQSSAIHHVLARHEQGAGFMAQGMARSTGLPAVCLASSGPGA
TNLLTAIADAKLDSIPLVAITGQVPKAMIGTDAFQEVDTYGLSIPITKHNFLVSSPEELLAVIPRAFEIATSGRPGPVLV
DIPKDVQTQAIEIGEWPEPGRAQPITPTDPALIAQATAMINAAERPILYLGGGVVHSGAARTAVDLAEKSNLPTVMTLMA
LGTMPVDHPLSLGMLGMHAARYTNLALEECDLLIAVGARFDDRATGKVAAFCPQAKIIHIDIDPAELDKIKTAHLGIAAD
VQEALTQLLPAVADNDRQVWVDRNNTLKAEFPFEMPGLDNPRSHFGLIRTVAACLDNEATVTTDVGQHQMWVAQAYPLRR
PRQWLTSGGLGTMGFGVPAAIGAALAEPQRTVVCFTGDGSILMNIQELVTAAEENVNVKIVLMDNATLGLVHQQQTLFYG
ERLFASQFKSSPDFIKIAQGFGIAAVDLDLAANPCAALMEAIARPGPCLIHASIDAEQKVYPMVPPGAANRDMIGA
>Mature_555_residues
TETLTGAQITVRLLERQGIRTVAGIPGGAILPIYDALGQSSAIHHVLARHEQGAGFMAQGMARSTGLPAVCLASSGPGAT
NLLTAIADAKLDSIPLVAITGQVPKAMIGTDAFQEVDTYGLSIPITKHNFLVSSPEELLAVIPRAFEIATSGRPGPVLVD
IPKDVQTQAIEIGEWPEPGRAQPITPTDPALIAQATAMINAAERPILYLGGGVVHSGAARTAVDLAEKSNLPTVMTLMAL
GTMPVDHPLSLGMLGMHAARYTNLALEECDLLIAVGARFDDRATGKVAAFCPQAKIIHIDIDPAELDKIKTAHLGIAADV
QEALTQLLPAVADNDRQVWVDRNNTLKAEFPFEMPGLDNPRSHFGLIRTVAACLDNEATVTTDVGQHQMWVAQAYPLRRP
RQWLTSGGLGTMGFGVPAAIGAALAEPQRTVVCFTGDGSILMNIQELVTAAEENVNVKIVLMDNATLGLVHQQQTLFYGE
RLFASQFKSSPDFIKIAQGFGIAAVDLDLAANPCAALMEAIARPGPCLIHASIDAEQKVYPMVPPGAANRDMIGA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=485, Percent_Identity=27.0103092783505, Blast_Score=149, Evalue=8e-36,
Organism=Homo sapiens, GI21361361, Length=545, Percent_Identity=23.8532110091743, Blast_Score=124, Evalue=3e-28,
Organism=Escherichia coli, GI1790104, Length=553, Percent_Identity=63.2911392405063, Blast_Score=712, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=41.8727915194346, Blast_Score=447, Evalue=1e-127,
Organism=Escherichia coli, GI1786717, Length=519, Percent_Identity=35.8381502890173, Blast_Score=308, Evalue=6e-85,
Organism=Escherichia coli, GI1787096, Length=551, Percent_Identity=27.2232304900181, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1788716, Length=481, Percent_Identity=28.2744282744283, Blast_Score=150, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17531299, Length=563, Percent_Identity=28.0639431616341, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17531301, Length=563, Percent_Identity=28.0639431616341, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=572, Percent_Identity=26.2237762237762, Blast_Score=133, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6323755, Length=576, Percent_Identity=41.8402777777778, Blast_Score=427, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6320816, Length=501, Percent_Identity=24.750499001996, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19922626, Length=483, Percent_Identity=27.9503105590062, Blast_Score=154, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 58751; Mature: 58620

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETLTGAQITVRLLERQGIRTVAGIPGGAILPIYDALGQSSAIHHVLARHEQGAGFMAQ
CCCCCCCHHHHHEEHHHCCCEEECCCCCCCEEHHHHHCCCCHHHHHHHHHCCCCCCHHHH
GMARSTGLPAVCLASSGPGATNLLTAIADAKLDSIPLVAITGQVPKAMIGTDAFQEVDTY
HHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHCCHHHHHHHHC
GLSIPITKHNFLVSSPEELLAVIPRAFEIATSGRPGPVLVDIPKDVQTQAIEIGEWPEPG
CCEEEEECCCEEECCHHHHHHHHCHHHEEECCCCCCCEEEECCCCCCCCEEECCCCCCCC
RAQPITPTDPALIAQATAMINAAERPILYLGGGVVHSGAARTAVDLAEKSNLPTVMTLMA
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEECCCHHHHHHHHHHCCCCHHHHHHH
LGTMPVDHPLSLGMLGMHAARYTNLALEECDLLIAVGARFDDRATGKVAAFCPQAKIIHI
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCEEEECCCCEEEEE
DIDPAELDKIKTAHLGIAADVQEALTQLLPAVADNDRQVWVDRNNTLKAEFPFEMPGLDN
ECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCCCCCCC
PRSHFGLIRTVAACLDNEATVTTDVGQHQMWVAQAYPLRRPRQWLTSGGLGTMGFGVPAA
CHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCHHHH
IGAALAEPQRTVVCFTGDGSILMNIQELVTAAEENVNVKIVLMDNATLGLVHQQQTLFYG
HHHHHCCCCEEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCCEEEEEECHHHHHHH
ERLFASQFKSSPDFIKIAQGFGIAAVDLDLAANPCAALMEAIARPGPCLIHASIDAEQKV
HHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCCCCE
YPMVPPGAANRDMIGA
ECCCCCCCCCCCCCCC
>Mature Secondary Structure 
TETLTGAQITVRLLERQGIRTVAGIPGGAILPIYDALGQSSAIHHVLARHEQGAGFMAQ
CCCCCCHHHHHEEHHHCCCEEECCCCCCCEEHHHHHCCCCHHHHHHHHHCCCCCCHHHH
GMARSTGLPAVCLASSGPGATNLLTAIADAKLDSIPLVAITGQVPKAMIGTDAFQEVDTY
HHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHCCHHHHHHHHC
GLSIPITKHNFLVSSPEELLAVIPRAFEIATSGRPGPVLVDIPKDVQTQAIEIGEWPEPG
CCEEEEECCCEEECCHHHHHHHHCHHHEEECCCCCCCEEEECCCCCCCCEEECCCCCCCC
RAQPITPTDPALIAQATAMINAAERPILYLGGGVVHSGAARTAVDLAEKSNLPTVMTLMA
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEECCCHHHHHHHHHHCCCCHHHHHHH
LGTMPVDHPLSLGMLGMHAARYTNLALEECDLLIAVGARFDDRATGKVAAFCPQAKIIHI
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCEEEECCCCEEEEE
DIDPAELDKIKTAHLGIAADVQEALTQLLPAVADNDRQVWVDRNNTLKAEFPFEMPGLDN
ECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCCCCCCC
PRSHFGLIRTVAACLDNEATVTTDVGQHQMWVAQAYPLRRPRQWLTSGGLGTMGFGVPAA
CHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCHHHH
IGAALAEPQRTVVCFTGDGSILMNIQELVTAAEENVNVKIVLMDNATLGLVHQQQTLFYG
HHHHHCCCCEEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCCEEEEEECHHHHHHH
ERLFASQFKSSPDFIKIAQGFGIAAVDLDLAANPCAALMEAIARPGPCLIHASIDAEQKV
HHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCCCCE
YPMVPPGAANRDMIGA
ECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2989782; 2989781; 7686882; 9278503 [H]