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The map label for this gene is ykfB [H]

Identifier: 71907246

GI number: 71907246

Start: 1733571

End: 1734527

Strand: Reverse

Name: ykfB [H]

Synonym: Daro_1615

Alternate gene names: 71907246

Gene position: 1734527-1733571 (Counterclockwise)

Preceding gene: 71907247

Following gene: 71907243

Centisome position: 38.54

GC content: 58.93

Gene sequence:

>957_bases
GTGAACATCGCTGCAGCCGACTGGCAGCCCTACCAGCTCCCGTTAAATCGGCCTTGGCAAACCAGCCGGGGAACATTATT
CGAGCGTTGCGGCCGCCTGCTTCGACTTCAGACAACAGACGGATTGACCGGCTGGGGTGATGCGGCCCCGCTACCTGAAT
TCGGCATCTGCGAAGCCGCGGCAACAGCCTTTGCCGACGAAACTGCGCACCTCGATCTGGCCGCCCAAAAAGCGGGGCTT
CCTCTCCATGCCTGGCTGAGCGGGGAAGTGCCTATTCTTTCCATCAGGACGAACGCCAACTTGGGTGCGATTGACACCGT
CGACCATGTCGCGCTCAAGAGTGCTGTCGACGCCGGTTTTTCCGTCGTGAAAATCAAGGTTGGCCGACAAGCACTGGCCG
ACGAGATTACCTACTTGCAGCAACTCACCCGGGATCTGCCGCCAACACTGAGCTTTCGTCTGGATGCAAACCAGGCTTGG
CAATTTGCCGATGCGCGAGCCTTCATCGAAGCCTGCGCCGTTCTGCCTATTGAAGGTTTGGAAGAACCGCTCAGCGATCC
TGATCCGGTCTTGCTCGCCAAATTACAGTCGTTTGCAGCATTCCCGCTCGCCATCGACGAATCGATTAACCTCCTCGATA
CGCATTTTTTCCGGCACCCACCGGTACGCCGCATCATCATCAAACCAGCTCGCCATGGCGGCCTGCTGGCCAGCATTGAA
CTGGCCTTGCGCGCCCGTGCTTCGAGCATCGAAACCATAATTACCTCAAGCCTTGAAAGCAGCTGCGGTCTGCTCGCCTG
CGCCCAACTAGCGGCGGCTGTGGCGCCAGATTCGATACACGGGCTGGCAACAGCAGACTGGTTTGCGCAAGATACGGGTA
TGGCTCCGACCATCATCGGCGGCCGCCTGCAGCTACCGGGCACTCCTGGCCTCGGTTTTCAACCCGTAACCCGATAA

Upstream 100 bases:

>100_bases
ACGCCACCGGCCTCTTCTACATGAGCGAGGAAGGGCAGGAAGGCCGCAATGCCTGGCTCGAACGCCGGCCACCCGATTTT
GGCAAGTACCGCAAGCGGCC

Downstream 100 bases:

>100_bases
GGGCCATCACCTGCCCGACAGCGAAACTGATAGCGAGCAAAGCATCGCCGCATCATCACGATTGCCGCCCCTGCTGCCGA
ATGCCATGACAAACGCGGAG

Product: mandelate racemase/muconate lactonizing protein

Products: O-Succinylbenzoate; H2O. [C]

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MNIAAADWQPYQLPLNRPWQTSRGTLFERCGRLLRLQTTDGLTGWGDAAPLPEFGICEAAATAFADETAHLDLAAQKAGL
PLHAWLSGEVPILSIRTNANLGAIDTVDHVALKSAVDAGFSVVKIKVGRQALADEITYLQQLTRDLPPTLSFRLDANQAW
QFADARAFIEACAVLPIEGLEEPLSDPDPVLLAKLQSFAAFPLAIDESINLLDTHFFRHPPVRRIIIKPARHGGLLASIE
LALRARASSIETIITSSLESSCGLLACAQLAAAVAPDSIHGLATADWFAQDTGMAPTIIGGRLQLPGTPGLGFQPVTR

Sequences:

>Translated_318_residues
MNIAAADWQPYQLPLNRPWQTSRGTLFERCGRLLRLQTTDGLTGWGDAAPLPEFGICEAAATAFADETAHLDLAAQKAGL
PLHAWLSGEVPILSIRTNANLGAIDTVDHVALKSAVDAGFSVVKIKVGRQALADEITYLQQLTRDLPPTLSFRLDANQAW
QFADARAFIEACAVLPIEGLEEPLSDPDPVLLAKLQSFAAFPLAIDESINLLDTHFFRHPPVRRIIIKPARHGGLLASIE
LALRARASSIETIITSSLESSCGLLACAQLAAAVAPDSIHGLATADWFAQDTGMAPTIIGGRLQLPGTPGLGFQPVTR
>Mature_318_residues
MNIAAADWQPYQLPLNRPWQTSRGTLFERCGRLLRLQTTDGLTGWGDAAPLPEFGICEAAATAFADETAHLDLAAQKAGL
PLHAWLSGEVPILSIRTNANLGAIDTVDHVALKSAVDAGFSVVKIKVGRQALADEITYLQQLTRDLPPTLSFRLDANQAW
QFADARAFIEACAVLPIEGLEEPLSDPDPVLLAKLQSFAAFPLAIDESINLLDTHFFRHPPVRRIIIKPARHGGLLASIE
LALRARASSIETIITSSLESSCGLLACAQLAAAVAPDSIHGLATADWFAQDTGMAPTIIGGRLQLPGTPGLGFQPVTR

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of the other Ala-X dipepti

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788596, Length=293, Percent_Identity=31.740614334471, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: 4.2.1.- [C]

Molecular weight: Translated: 34032; Mature: 34032

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIAAADWQPYQLPLNRPWQTSRGTLFERCGRLLRLQTTDGLTGWGDAAPLPEFGICEAA
CCCCCCCCCCEECCCCCCCCCCCCHHHHHCCHHEEEEECCCCCCCCCCCCCCCCCHHHHH
ATAFADETAHLDLAAQKAGLPLHAWLSGEVPILSIRTNANLGAIDTVDHVALKSAVDAGF
HHHHHCCCHHEEHHHHHCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCC
SVVKIKVGRQALADEITYLQQLTRDLPPTLSFRLDANQAWQFADARAFIEACAVLPIEGL
EEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCHHHH
EEPLSDPDPVLLAKLQSFAAFPLAIDESINLLDTHFFRHPPVRRIIIKPARHGGLLASIE
HHCCCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHCCCCCEEEEEECCCCCCEEEHHH
LALRARASSIETIITSSLESSCGLLACAQLAAAVAPDSIHGLATADWFAQDTGMAPTIIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCEEEC
GRLQLPGTPGLGFQPVTR
CEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MNIAAADWQPYQLPLNRPWQTSRGTLFERCGRLLRLQTTDGLTGWGDAAPLPEFGICEAA
CCCCCCCCCCEECCCCCCCCCCCCHHHHHCCHHEEEEECCCCCCCCCCCCCCCCCHHHHH
ATAFADETAHLDLAAQKAGLPLHAWLSGEVPILSIRTNANLGAIDTVDHVALKSAVDAGF
HHHHHCCCHHEEHHHHHCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCC
SVVKIKVGRQALADEITYLQQLTRDLPPTLSFRLDANQAWQFADARAFIEACAVLPIEGL
EEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCHHHH
EEPLSDPDPVLLAKLQSFAAFPLAIDESINLLDTHFFRHPPVRRIIIKPARHGGLLASIE
HHCCCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHCCCCCEEEEEECCCCCCEEEHHH
LALRARASSIETIITSSLESSCGLLACAQLAAAVAPDSIHGLATADWFAQDTGMAPTIIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCEEEC
GRLQLPGTPGLGFQPVTR
CEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: @SUCC01.txt*2-Succinyl-6-Hydroxy-2,4-Cyclohexadiene-1- Carboxylate! [C]

Specific reaction: @SUCC01.txt*2-Succinyl-6-Hydroxy-2,4-Cyclohexadiene-1- Carboxylate! = O-Succinylbenzoate + H2O. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]