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The map label for this gene is nifA [H]

Identifier: 71907119

GI number: 71907119

Start: 1603477

End: 1605126

Strand: Reverse

Name: nifA [H]

Synonym: Daro_1487

Alternate gene names: 71907119

Gene position: 1605126-1603477 (Counterclockwise)

Preceding gene: 71907120

Following gene: 71907117

Centisome position: 35.66

GC content: 63.82

Gene sequence:

>1650_bases
GTGACCCGATCTAAGAAAAACCACGAGAACAGCATGCACGAACACACCATCCACCCGCTTGGCGATGAATGCCCGCCCAC
CGGCGGCTTCTGCCGAACGCACGAGCTGAACATCCAGCTGCTGGCCGCGCTCTACGCCGTCAGCCGGGTGCTCAGCCGCT
CCCTCGACTTCAACGAAACGCTGCGCGATGTGCTGCGCGTGCTGCACGACGAGGCCGGACTGACCCGTGGCCTGATCAGC
GTGGTGGACCCGGACAGCGGCAAGCTGAACATCCACACCATCTACACGCCGGAAGGCCCGATCCTCGACGACAACCAGTA
CGGCCCGGGCGAAGGCGTCATTGGTCTGGTCCTCGAAAAACCACGCACCATCAAACTGGCCCGCGGCGCCGATGAGCCGC
ATTTCCTGAACCGCCACGGTGTCTATCAGCCCGACTTGCCCTTTATCGCCGTACCGATCAAGGTCGGCGGCGACCTGAAG
GGCGTCCTCGCCGTCCAGCCGGAAGCGCCGGAAGACGGCTTACTGGAAGAGCGCGCCCAGTTCGTCGAAATGGTTTCCAA
CCTGATCGGCCAGAGCTTGCGGCTGGCGATGGACGTTGCCCAGGAAAAATCGACCCTGCTCGAGGAGCGTGACCTGCTCC
GGCGCACCGTGCGCCACCAGTTCGGCTTCGACAGCATGGTCGGTCGCTCGGCCGTGATGCGCCGCGTCTTCGACCAGGCA
CGCATGGTCGCCAAGTGGAATACCACGGTACTGATCCGCGGCGAAACCGGCACCGGCAAGGAACTGATCGCCAATGCCAT
CCACTACAACTCGCCGCGCGCCCGCAACGCGCTGGTCAAGCTCAACTGCGCCGCGCTGCCGGAAAACCTGCTTGAGTCGG
AGCTCTTCGGCCACGAGCGCGGCGCCTTCACCGGTGCCGTCGAGTCACGCAAGGGTCGCTTCGAGCAAGCCCACGGCGGC
ACCCTCTTTCTCGATGAAATCGGCGAGGTTTCGCCAGCTTTCCAGGCCAAGATGCTGCGCATCCTGCAGGAAGGCGAATT
CGAGCGAGTCGGCGGCAGCAAGACGATCAAGGTCGATGTCCGCATCATCGCCGCCACCCACCGCGATCTGGAAACCGCCG
TTGAAATGGGCGACTTCCGCGAAGACTTGTTCTACCGCCTCAACGTCATGCCCCTCTTCCTGCCGCCGCTGCGCGAACGG
ATCGAGGACATCCCGGAAATCGCCCGTCACCTGCTCGGCAAGATCGGCAACGACCAGAAGCGCAAGCTGACCCTGACCGA
CATGGCCAATCGCCGCCTGGCCAGCCACGAATGGCCGGGCAATGTCCGTGAGCTGGAAAACTGCCTGGAACGCGCTGCGG
TGTTGTCCGACGATGGCCACATCGATGTCGACCTGATCCGCTTCCCCAGCGCCCGCGAACGAAGCAGCCCCCGCCCCCTG
CGCACCGCCCCATCAGCGTTCAGCCCGGCTTCTGCCTCAAGCCCGGAAATCGACATCGACGACCCGAACCTCTCGGAAAA
AGAACGCGTCATTGCGGCGCTGGAACAGGCAGGCTGGGTCCAGGCCAAGGCGGCGCGCATTCTCGGCATGACGCCTCGGC
AGATCGCCTACCGGATTCAGACGCTGAATATCGAGGTCAAGCAGTTCTAG

Upstream 100 bases:

>100_bases
TCGCGCGCCCAGCAGGTGGTGGCCGATCACGGCGGCATCATCGATCTCGATGACAGCCCGAGCGGCGGTTGCGCGGCGAT
TGTCGAATTCCGGGTCGACG

Downstream 100 bases:

>100_bases
GCTTCTTTGGCGGCCAGGGCCAAGCCGCCTGGACTTACCAGGCGCGGGCACCCATCAGGTAGCTGCGGGTTTCCTGCTTG
ACGGGAGGCTCCGCTACCGG

Product: helix-turn-helix, Fis-type:Nif-specific regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MTRSKKNHENSMHEHTIHPLGDECPPTGGFCRTHELNIQLLAALYAVSRVLSRSLDFNETLRDVLRVLHDEAGLTRGLIS
VVDPDSGKLNIHTIYTPEGPILDDNQYGPGEGVIGLVLEKPRTIKLARGADEPHFLNRHGVYQPDLPFIAVPIKVGGDLK
GVLAVQPEAPEDGLLEERAQFVEMVSNLIGQSLRLAMDVAQEKSTLLEERDLLRRTVRHQFGFDSMVGRSAVMRRVFDQA
RMVAKWNTTVLIRGETGTGKELIANAIHYNSPRARNALVKLNCAALPENLLESELFGHERGAFTGAVESRKGRFEQAHGG
TLFLDEIGEVSPAFQAKMLRILQEGEFERVGGSKTIKVDVRIIAATHRDLETAVEMGDFREDLFYRLNVMPLFLPPLRER
IEDIPEIARHLLGKIGNDQKRKLTLTDMANRRLASHEWPGNVRELENCLERAAVLSDDGHIDVDLIRFPSARERSSPRPL
RTAPSAFSPASASSPEIDIDDPNLSEKERVIAALEQAGWVQAKAARILGMTPRQIAYRIQTLNIEVKQF

Sequences:

>Translated_549_residues
MTRSKKNHENSMHEHTIHPLGDECPPTGGFCRTHELNIQLLAALYAVSRVLSRSLDFNETLRDVLRVLHDEAGLTRGLIS
VVDPDSGKLNIHTIYTPEGPILDDNQYGPGEGVIGLVLEKPRTIKLARGADEPHFLNRHGVYQPDLPFIAVPIKVGGDLK
GVLAVQPEAPEDGLLEERAQFVEMVSNLIGQSLRLAMDVAQEKSTLLEERDLLRRTVRHQFGFDSMVGRSAVMRRVFDQA
RMVAKWNTTVLIRGETGTGKELIANAIHYNSPRARNALVKLNCAALPENLLESELFGHERGAFTGAVESRKGRFEQAHGG
TLFLDEIGEVSPAFQAKMLRILQEGEFERVGGSKTIKVDVRIIAATHRDLETAVEMGDFREDLFYRLNVMPLFLPPLRER
IEDIPEIARHLLGKIGNDQKRKLTLTDMANRRLASHEWPGNVRELENCLERAAVLSDDGHIDVDLIRFPSARERSSPRPL
RTAPSAFSPASASSPEIDIDDPNLSEKERVIAALEQAGWVQAKAARILGMTPRQIAYRIQTLNIEVKQF
>Mature_548_residues
TRSKKNHENSMHEHTIHPLGDECPPTGGFCRTHELNIQLLAALYAVSRVLSRSLDFNETLRDVLRVLHDEAGLTRGLISV
VDPDSGKLNIHTIYTPEGPILDDNQYGPGEGVIGLVLEKPRTIKLARGADEPHFLNRHGVYQPDLPFIAVPIKVGGDLKG
VLAVQPEAPEDGLLEERAQFVEMVSNLIGQSLRLAMDVAQEKSTLLEERDLLRRTVRHQFGFDSMVGRSAVMRRVFDQAR
MVAKWNTTVLIRGETGTGKELIANAIHYNSPRARNALVKLNCAALPENLLESELFGHERGAFTGAVESRKGRFEQAHGGT
LFLDEIGEVSPAFQAKMLRILQEGEFERVGGSKTIKVDVRIIAATHRDLETAVEMGDFREDLFYRLNVMPLFLPPLRERI
EDIPEIARHLLGKIGNDQKRKLTLTDMANRRLASHEWPGNVRELENCLERAAVLSDDGHIDVDLIRFPSARERSSPRPLR
TAPSAFSPASASSPEIDIDDPNLSEKERVIAALEQAGWVQAKAARILGMTPRQIAYRIQTLNIEVKQF

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=532, Percent_Identity=37.218045112782, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI1790437, Length=321, Percent_Identity=46.7289719626168, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI1788550, Length=355, Percent_Identity=44.7887323943662, Blast_Score=274, Evalue=8e-75,
Organism=Escherichia coli, GI1789087, Length=327, Percent_Identity=45.565749235474, Blast_Score=258, Evalue=8e-70,
Organism=Escherichia coli, GI1788905, Length=244, Percent_Identity=50.4098360655738, Blast_Score=246, Evalue=4e-66,
Organism=Escherichia coli, GI1790299, Length=333, Percent_Identity=43.2432432432432, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI87082152, Length=232, Percent_Identity=53.448275862069, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI87081872, Length=317, Percent_Identity=41.9558359621451, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1787583, Length=256, Percent_Identity=44.53125, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1786524, Length=262, Percent_Identity=44.2748091603053, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1789233, Length=244, Percent_Identity=43.4426229508197, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1789828, Length=322, Percent_Identity=36.6459627329193, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI87081858, Length=349, Percent_Identity=30.3724928366762, Blast_Score=139, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 61302; Mature: 61171

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRSKKNHENSMHEHTIHPLGDECPPTGGFCRTHELNIQLLAALYAVSRVLSRSLDFNET
CCCCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCHHHH
LRDVLRVLHDEAGLTRGLISVVDPDSGKLNIHTIYTPEGPILDDNQYGPGEGVIGLVLEK
HHHHHHHHHHHCCHHHHHHEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCEEEEEECC
PRTIKLARGADEPHFLNRHGVYQPDLPFIAVPIKVGGDLKGVLAVQPEAPEDGLLEERAQ
CCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEECCCCCCCCHHHHHHH
FVEMVSNLIGQSLRLAMDVAQEKSTLLEERDLLRRTVRHQFGFDSMVGRSAVMRRVFDQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
RMVAKWNTTVLIRGETGTGKELIANAIHYNSPRARNALVKLNCAALPENLLESELFGHER
HHHEECCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCC
GAFTGAVESRKGRFEQAHGGTLFLDEIGEVSPAFQAKMLRILQEGEFERVGGSKTIKVDV
CCCCCCHHHHCCCCHHCCCCEEEEHHHCCCCHHHHHHHHHHHHCCCHHHCCCCEEEEEEE
RIIAATHRDLETAVEMGDFREDLFYRLNVMPLFLPPLRERIEDIPEIARHLLGKIGNDQK
EEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
RKLTLTDMANRRLASHEWPGNVRELENCLERAAVLSDDGHIDVDLIRFPSARERSSPRPL
CEEEHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCC
RTAPSAFSPASASSPEIDIDDPNLSEKERVIAALEQAGWVQAKAARILGMTPRQIAYRIQ
CCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHEEEE
TLNIEVKQF
EEEEEEECC
>Mature Secondary Structure 
TRSKKNHENSMHEHTIHPLGDECPPTGGFCRTHELNIQLLAALYAVSRVLSRSLDFNET
CCCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCHHHH
LRDVLRVLHDEAGLTRGLISVVDPDSGKLNIHTIYTPEGPILDDNQYGPGEGVIGLVLEK
HHHHHHHHHHHCCHHHHHHEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCEEEEEECC
PRTIKLARGADEPHFLNRHGVYQPDLPFIAVPIKVGGDLKGVLAVQPEAPEDGLLEERAQ
CCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEECCCCCCCCHHHHHHH
FVEMVSNLIGQSLRLAMDVAQEKSTLLEERDLLRRTVRHQFGFDSMVGRSAVMRRVFDQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
RMVAKWNTTVLIRGETGTGKELIANAIHYNSPRARNALVKLNCAALPENLLESELFGHER
HHHEECCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCC
GAFTGAVESRKGRFEQAHGGTLFLDEIGEVSPAFQAKMLRILQEGEFERVGGSKTIKVDV
CCCCCCHHHHCCCCHHCCCCEEEEHHHCCCCHHHHHHHHHHHHCCCHHHCCCCEEEEEEE
RIIAATHRDLETAVEMGDFREDLFYRLNVMPLFLPPLRERIEDIPEIARHLLGKIGNDQK
EEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
RKLTLTDMANRRLASHEWPGNVRELENCLERAAVLSDDGHIDVDLIRFPSARERSSPRPL
CEEEHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCC
RTAPSAFSPASASSPEIDIDDPNLSEKERVIAALEQAGWVQAKAARILGMTPRQIAYRIQ
CCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHEEEE
TLNIEVKQF
EEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2840552; 2450865 [H]