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The map label for this gene is nifK [H]

Identifier: 71907045

GI number: 71907045

Start: 1541439

End: 1543007

Strand: Reverse

Name: nifK [H]

Synonym: Daro_1413

Alternate gene names: 71907045

Gene position: 1543007-1541439 (Counterclockwise)

Preceding gene: 71907046

Following gene: 71907044

Centisome position: 34.28

GC content: 59.66

Gene sequence:

>1569_bases
ATGCAAACCGCAGACAACATCAAGCCCTGTTACCCGCTGTTCCGCGACGACGACTACAAGAAGAACCTCGCTGAAAAACG
CGAACTCTTCGAAGAAGGCCACGGCCCGGAAAAGGTCTGGGAAACCTTCGTCTGGACGACGACCAAGGAATATCAGGAAC
TCAACTTCAAGCGCGAAGCCCTGACCGTCGATCCGGCCAAGGCCTGCCAGCCGCTCGGCGCCGTCCTCTGCGCCTCCGGC
TTCCACAAGACCCTGCCCTACGTGCATGGCTCGCAAGGTTGTGTCGCCTATTTCCGCACCTACTTCAACCGTCACTTCAA
GGAACCGTGCTCCTGCGTGTCCGACTCGATGACGGAAGACGCCGCCGTGTTCGGCGGGCAGAAGAACATGCACGACGGTC
TGGCCAACGCCAAGGCCATCTACAAGCCGGACATGATCGCTGTCTCGACCACCTGCATGGCCGAAGTCATCGGTGACGAC
CTGAATGCCTTCATCAACAACTCCAAGAAGGACGGCCACATCCCCCAGGATTACCCGGTCCCCTTCGCCCACACCCCGTC
CTTCGTCGGTTCGCACACTACCGGCTGGGACAACATGCTCGAAGGCATCATCCGTTCCTTCACGCTGAACTTCATGGACG
GCAAGAAGGTCGGTTCGAATGGCAAGATCAACATCGTGCCGGGCTTCGAGACCTACCTCGGCAACTACCGTGTCATCAAG
CGCATGCTCGGTGAAATGGATGTCGATTTCACCTACCTGTCCGATCCTTCCGAAGTCTTCGACACCCCGGCCGACGGCGA
ATTCCGCATGTACTCCGGCGGCACGACGATGGACGAGGTCAAGGATGCGCCGAACGCCTACACCACCATCCTGCTGCAGC
CCACCCAGCTGGAAAAGACGAAGAAATTCGTCGAAGCCACCTGGAACCAGGACATCCCCAAGCTCAACATCCCGATGGGC
GTGGAATGGACCGACGAGTTCCTGATGAAGGTCTCCGAGATCACCGGCAAGCCGATCCCGGCCTCGCTGGAACTGGAACG
CGGCCGCTGCATGGACCTCGTCTCCGACAGCCACGCCTGGCTGCACGGCAAGAAGTTTGCCGTGTATGGTGACCCGGACT
TCGTGATGGGCGTCGTCAAGATCCTGCTCGAAGTCGGTGCCGAGCCGACCCACATCCTGGCCCACAACGGCAACAAGCGT
TGGGCCAAGACCATGGACAAGCTCCTCGCCTCCAGCCCGTTCGGTGTCAATGGCAAGGTGTATGCCGGCAACGACCTGTG
GCACATGCGCAGCCTGTGCTTCACGGACAAGCCGGATTTCCTGATCGGCAACTCCTACGGCAAGTACATCCAGCGCGACA
CCCGCCACCTGGGTGAAGAGTTCGAAGTACCGCTGATCCGCCTCGGCTTCCCGATCTTCGACCGCCATCACATGCACCGC
GCGACCACCATGGGCTACGAAGGGATGATGTCCGTAGTGACCCAGCTGACCAACGCTGTCCTTGAGCAACTCGACAAGGA
AACGATCGGCATGGGCACCACCGACTTCAACTTCGACCTGGTTCGCTAA

Upstream 100 bases:

>100_bases
TTGCTGCTGAAGAAGTCAAGAAGGCCGCCTGACCTAGCTCAGTAAATCCTTAACCTTGAGCCCGCAGCCGCGAATGAGCG
GGGCGGGCAAGGAGATAGCA

Downstream 100 bases:

>100_bases
ACGGACCACCGGCGGAGCCCTGGCTCCGCCGGTCATGCAGGAGCGTTACATGGCCAACATCATGATCCAGCAAGGCGACA
AGGGCGGGCTGGTGTTTTAC

Product: nitrogenase molybdenum-iron protein beta chain

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MQTADNIKPCYPLFRDDDYKKNLAEKRELFEEGHGPEKVWETFVWTTTKEYQELNFKREALTVDPAKACQPLGAVLCASG
FHKTLPYVHGSQGCVAYFRTYFNRHFKEPCSCVSDSMTEDAAVFGGQKNMHDGLANAKAIYKPDMIAVSTTCMAEVIGDD
LNAFINNSKKDGHIPQDYPVPFAHTPSFVGSHTTGWDNMLEGIIRSFTLNFMDGKKVGSNGKINIVPGFETYLGNYRVIK
RMLGEMDVDFTYLSDPSEVFDTPADGEFRMYSGGTTMDEVKDAPNAYTTILLQPTQLEKTKKFVEATWNQDIPKLNIPMG
VEWTDEFLMKVSEITGKPIPASLELERGRCMDLVSDSHAWLHGKKFAVYGDPDFVMGVVKILLEVGAEPTHILAHNGNKR
WAKTMDKLLASSPFGVNGKVYAGNDLWHMRSLCFTDKPDFLIGNSYGKYIQRDTRHLGEEFEVPLIRLGFPIFDRHHMHR
ATTMGYEGMMSVVTQLTNAVLEQLDKETIGMGTTDFNFDLVR

Sequences:

>Translated_522_residues
MQTADNIKPCYPLFRDDDYKKNLAEKRELFEEGHGPEKVWETFVWTTTKEYQELNFKREALTVDPAKACQPLGAVLCASG
FHKTLPYVHGSQGCVAYFRTYFNRHFKEPCSCVSDSMTEDAAVFGGQKNMHDGLANAKAIYKPDMIAVSTTCMAEVIGDD
LNAFINNSKKDGHIPQDYPVPFAHTPSFVGSHTTGWDNMLEGIIRSFTLNFMDGKKVGSNGKINIVPGFETYLGNYRVIK
RMLGEMDVDFTYLSDPSEVFDTPADGEFRMYSGGTTMDEVKDAPNAYTTILLQPTQLEKTKKFVEATWNQDIPKLNIPMG
VEWTDEFLMKVSEITGKPIPASLELERGRCMDLVSDSHAWLHGKKFAVYGDPDFVMGVVKILLEVGAEPTHILAHNGNKR
WAKTMDKLLASSPFGVNGKVYAGNDLWHMRSLCFTDKPDFLIGNSYGKYIQRDTRHLGEEFEVPLIRLGFPIFDRHHMHR
ATTMGYEGMMSVVTQLTNAVLEQLDKETIGMGTTDFNFDLVR
>Mature_522_residues
MQTADNIKPCYPLFRDDDYKKNLAEKRELFEEGHGPEKVWETFVWTTTKEYQELNFKREALTVDPAKACQPLGAVLCASG
FHKTLPYVHGSQGCVAYFRTYFNRHFKEPCSCVSDSMTEDAAVFGGQKNMHDGLANAKAIYKPDMIAVSTTCMAEVIGDD
LNAFINNSKKDGHIPQDYPVPFAHTPSFVGSHTTGWDNMLEGIIRSFTLNFMDGKKVGSNGKINIVPGFETYLGNYRVIK
RMLGEMDVDFTYLSDPSEVFDTPADGEFRMYSGGTTMDEVKDAPNAYTTILLQPTQLEKTKKFVEATWNQDIPKLNIPMG
VEWTDEFLMKVSEITGKPIPASLELERGRCMDLVSDSHAWLHGKKFAVYGDPDFVMGVVKILLEVGAEPTHILAHNGNKR
WAKTMDKLLASSPFGVNGKVYAGNDLWHMRSLCFTDKPDFLIGNSYGKYIQRDTRHLGEEFEVPLIRLGFPIFDRHHMHR
ATTMGYEGMMSVVTQLTNAVLEQLDKETIGMGTTDFNFDLVR

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005976 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 58837; Mature: 58837

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTADNIKPCYPLFRDDDYKKNLAEKRELFEEGHGPEKVWETFVWTTTKEYQELNFKREA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHCCCHHHH
LTVDPAKACQPLGAVLCASGFHKTLPYVHGSQGCVAYFRTYFNRHFKEPCSCVSDSMTED
EECCHHHHHHHHHHHHHHCCHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHH
AAVFGGQKNMHDGLANAKAIYKPDMIAVSTTCMAEVIGDDLNAFINNSKKDGHIPQDYPV
HHHHCCCCCHHHHHHCCCEEECCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PFAHTPSFVGSHTTGWDNMLEGIIRSFTLNFMDGKKVGSNGKINIVPGFETYLGNYRVIK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHCCHHHHH
RMLGEMDVDFTYLSDPSEVFDTPADGEFRMYSGGTTMDEVKDAPNAYTTILLQPTQLEKT
HHHCCCCCCEEEECCCHHHHCCCCCCCEEEEECCCCHHHHHCCCCCEEEEEECCHHHHHH
KKFVEATWNQDIPKLNIPMGVEWTDEFLMKVSEITGKPIPASLELERGRCMDLVSDSHAW
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHCCCCCE
LHGKKFAVYGDPDFVMGVVKILLEVGAEPTHILAHNGNKRWAKTMDKLLASSPFGVNGKV
ECCCEEEEECCHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCEE
YAGNDLWHMRSLCFTDKPDFLIGNSYGKYIQRDTRHLGEEFEVPLIRLGFPIFDRHHMHR
EECCCHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCHHCCCCEEEECCCHHHHHHHHH
ATTMGYEGMMSVVTQLTNAVLEQLDKETIGMGTTDFNFDLVR
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECC
>Mature Secondary Structure
MQTADNIKPCYPLFRDDDYKKNLAEKRELFEEGHGPEKVWETFVWTTTKEYQELNFKREA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHCCCHHHH
LTVDPAKACQPLGAVLCASGFHKTLPYVHGSQGCVAYFRTYFNRHFKEPCSCVSDSMTED
EECCHHHHHHHHHHHHHHCCHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHH
AAVFGGQKNMHDGLANAKAIYKPDMIAVSTTCMAEVIGDDLNAFINNSKKDGHIPQDYPV
HHHHCCCCCHHHHHHCCCEEECCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PFAHTPSFVGSHTTGWDNMLEGIIRSFTLNFMDGKKVGSNGKINIVPGFETYLGNYRVIK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHCCHHHHH
RMLGEMDVDFTYLSDPSEVFDTPADGEFRMYSGGTTMDEVKDAPNAYTTILLQPTQLEKT
HHHCCCCCCEEEECCCHHHHCCCCCCCEEEEECCCCHHHHHCCCCCEEEEEECCHHHHHH
KKFVEATWNQDIPKLNIPMGVEWTDEFLMKVSEITGKPIPASLELERGRCMDLVSDSHAW
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHCCCCCE
LHGKKFAVYGDPDFVMGVVKILLEVGAEPTHILAHNGNKRWAKTMDKLLASSPFGVNGKV
ECCCEEEEECCHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCEE
YAGNDLWHMRSLCFTDKPDFLIGNSYGKYIQRDTRHLGEEFEVPLIRLGFPIFDRHHMHR
EECCCHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCHHCCCCEEEECCCHHHHHHHHH
ATTMGYEGMMSVVTQLTNAVLEQLDKETIGMGTTDFNFDLVR
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644218; 3863780; 649581; 9163420; 9063865; 11170380; 11951047; 12215645 [H]