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The map label for this gene is rpfG [H]

Identifier: 71906609

GI number: 71906609

Start: 1054536

End: 1055852

Strand: Reverse

Name: rpfG [H]

Synonym: Daro_0970

Alternate gene names: 71906609

Gene position: 1055852-1054536 (Counterclockwise)

Preceding gene: 71906610

Following gene: 71906608

Centisome position: 23.46

GC content: 62.87

Gene sequence:

>1317_bases
ATGAGCGAAACACCCGCCCCCGCCACCCTGCTCTTCGTCGATGACGAACCGGGCATCCTTTCGGCCCTGCGCCGCCTGTT
CCGGCCGCATGGCTATCGCATCCTGATTGCCGAAAGCGGTGCCGCCGGCCTCAGCATCCTGGAGCAGGAGGCGGTCGACC
TGATCATTTCCGATATGCGCATGCCGGAAATGGATGGCGCCACCTTCCTCAAGCAGGTGCGCGGCCGCTGGCCGAACACG
ATGCGGATCCTGCTCACCGGCTACGCCGACGTCACCTCGACTGTCGCCGCCATCAACGAGGGCGAAATCTACCGCTACAT
TGCCAAACCCTGGGACGACGCCGAAATCGTCAACACGGTTCGGAATGCGCTGGAACGCCAGCAGCTGGAAGTGGAAAACC
GTCGCCTGACCGCGCTGACCCAGGCCCAGAACGAAGAACTGAAGGTGCTCAACGTTAATCTGGAGCAGAAGGTCGCCGAA
CGTACCGCCGAATTGCAGCAGGCGCTGACCTTTGTCGAGCAGTCGCACGGCGAACTCAAGAAATCCTTCCTGACCAGCGT
GCAGGTTTTCGCCGGCCTGATCGAGCTGCGGGCTGGCCAGAGCGGCAGCCGGATGGCCGGCCACGGCCGCCGCGTCGCTG
ACATGGCACGCACATTGGCCCAGCGCCTCGAACTGAGCGAGGTCGATATCCAGAACGTCATGCTTGCCGGCCTGCTGCAC
GACATTGGCAAACTCGGCCTGTCCGACGAAATCCTCAGCAAATCGTTCAATACGCTCAGCGCCGAGCAGCGGGCGCAGGT
CATGAAACACCCGCAGATCGGCCAAAACCTGCTGATGGCCATCGACCGTTGCAAGGAGGCGGCCATCCTTGTTCGCCACC
ACCACGAATGCTTCGACGGCAGCGGTTACCCGGACCGCCTGGCCGGCATTGCGATCCCGCTTGGCGCCCGCATTCTGGCC
GTGGTCAACGACTACGACGCCCTGCAGATCGGCACCCTGGTCCAGCGTCCCCTGCGCCCGGAAGAAGCACTGGCCTTCCT
GATCGACAATCGCGGCAAGCGCTACGATCCGACGGCGGTCGAGGCTTTCGTGCGCCTGATCGGCGAAACCCAGAAGAGCG
GCCCGGTCGAGTTGCCATTACGCACCATGCACCTGAAGCCGGGCATGGTCCTGACCCGTGAACTGGCTCACCGTGACGGC
TATCTGCTGCTGGCCAAGGGCAGTGTGCTGACGACGGATATCATCAATCAACTGGTCAGGCTGGAGCAGGCCGAACAGCA
CAACCTGACCCTCTACACCCGCTCGGAGGAAAAATGA

Upstream 100 bases:

>100_bases
ATCACGGTCGCATCGAGGTCGACAGTCAGCTCGGTGTCGGCTCGACCTTCCGCATCATTCTTCCCATCAATCAGAAAAAC
AGCCCACCGGAGACTTCGCG

Downstream 100 bases:

>100_bases
GCCGTATCATGCTCGTCGACGACGAAGAATCGATCCTCAAGTCCCTGAAACGCATCCTGCGCCTGGCCCCCTGTGCCTTT
GGCGGCAAGACCTTCCAGCT

Product: response regulator receiver

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MSETPAPATLLFVDDEPGILSALRRLFRPHGYRILIAESGAAGLSILEQEAVDLIISDMRMPEMDGATFLKQVRGRWPNT
MRILLTGYADVTSTVAAINEGEIYRYIAKPWDDAEIVNTVRNALERQQLEVENRRLTALTQAQNEELKVLNVNLEQKVAE
RTAELQQALTFVEQSHGELKKSFLTSVQVFAGLIELRAGQSGSRMAGHGRRVADMARTLAQRLELSEVDIQNVMLAGLLH
DIGKLGLSDEILSKSFNTLSAEQRAQVMKHPQIGQNLLMAIDRCKEAAILVRHHHECFDGSGYPDRLAGIAIPLGARILA
VVNDYDALQIGTLVQRPLRPEEALAFLIDNRGKRYDPTAVEAFVRLIGETQKSGPVELPLRTMHLKPGMVLTRELAHRDG
YLLLAKGSVLTTDIINQLVRLEQAEQHNLTLYTRSEEK

Sequences:

>Translated_438_residues
MSETPAPATLLFVDDEPGILSALRRLFRPHGYRILIAESGAAGLSILEQEAVDLIISDMRMPEMDGATFLKQVRGRWPNT
MRILLTGYADVTSTVAAINEGEIYRYIAKPWDDAEIVNTVRNALERQQLEVENRRLTALTQAQNEELKVLNVNLEQKVAE
RTAELQQALTFVEQSHGELKKSFLTSVQVFAGLIELRAGQSGSRMAGHGRRVADMARTLAQRLELSEVDIQNVMLAGLLH
DIGKLGLSDEILSKSFNTLSAEQRAQVMKHPQIGQNLLMAIDRCKEAAILVRHHHECFDGSGYPDRLAGIAIPLGARILA
VVNDYDALQIGTLVQRPLRPEEALAFLIDNRGKRYDPTAVEAFVRLIGETQKSGPVELPLRTMHLKPGMVLTRELAHRDG
YLLLAKGSVLTTDIINQLVRLEQAEQHNLTLYTRSEEK
>Mature_437_residues
SETPAPATLLFVDDEPGILSALRRLFRPHGYRILIAESGAAGLSILEQEAVDLIISDMRMPEMDGATFLKQVRGRWPNTM
RILLTGYADVTSTVAAINEGEIYRYIAKPWDDAEIVNTVRNALERQQLEVENRRLTALTQAQNEELKVLNVNLEQKVAER
TAELQQALTFVEQSHGELKKSFLTSVQVFAGLIELRAGQSGSRMAGHGRRVADMARTLAQRLELSEVDIQNVMLAGLLHD
IGKLGLSDEILSKSFNTLSAEQRAQVMKHPQIGQNLLMAIDRCKEAAILVRHHHECFDGSGYPDRLAGIAIPLGARILAV
VNDYDALQIGTLVQRPLRPEEALAFLIDNRGKRYDPTAVEAFVRLIGETQKSGPVELPLRTMHLKPGMVLTRELAHRDGY
LLLAKGSVLTTDIINQLVRLEQAEQHNLTLYTRSEEK

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=128, Percent_Identity=38.28125, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788550, Length=127, Percent_Identity=35.4330708661417, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1790299, Length=127, Percent_Identity=33.0708661417323, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1790437, Length=115, Percent_Identity=33.0434782608696, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 48802; Mature: 48671

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETPAPATLLFVDDEPGILSALRRLFRPHGYRILIAESGAAGLSILEQEAVDLIISDMR
CCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCC
MPEMDGATFLKQVRGRWPNTMRILLTGYADVTSTVAAINEGEIYRYIAKPWDDAEIVNTV
CCCCCHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHH
RNALERQQLEVENRRLTALTQAQNEELKVLNVNLEQKVAERTAELQQALTFVEQSHGELK
HHHHHHHHHHHHCCHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSFLTSVQVFAGLIELRAGQSGSRMAGHGRRVADMARTLAQRLELSEVDIQNVMLAGLLH
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
DIGKLGLSDEILSKSFNTLSAEQRAQVMKHPQIGQNLLMAIDRCKEAAILVRHHHECFDG
HHHHCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
SGYPDRLAGIAIPLGARILAVVNDYDALQIGTLVQRPLRPEEALAFLIDNRGKRYDPTAV
CCCCHHHHHEEECCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHH
EAFVRLIGETQKSGPVELPLRTMHLKPGMVLTRELAHRDGYLLLAKGSVLTTDIINQLVR
HHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHH
LEQAEQHNLTLYTRSEEK
HHHHHCCCEEEEECCCCC
>Mature Secondary Structure 
SETPAPATLLFVDDEPGILSALRRLFRPHGYRILIAESGAAGLSILEQEAVDLIISDMR
CCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCC
MPEMDGATFLKQVRGRWPNTMRILLTGYADVTSTVAAINEGEIYRYIAKPWDDAEIVNTV
CCCCCHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHH
RNALERQQLEVENRRLTALTQAQNEELKVLNVNLEQKVAERTAELQQALTFVEQSHGELK
HHHHHHHHHHHHCCHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSFLTSVQVFAGLIELRAGQSGSRMAGHGRRVADMARTLAQRLELSEVDIQNVMLAGLLH
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
DIGKLGLSDEILSKSFNTLSAEQRAQVMKHPQIGQNLLMAIDRCKEAAILVRHHHECFDG
HHHHCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
SGYPDRLAGIAIPLGARILAVVNDYDALQIGTLVQRPLRPEEALAFLIDNRGKRYDPTAV
CCCCHHHHHEEECCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHH
EAFVRLIGETQKSGPVELPLRTMHLKPGMVLTRELAHRDGYLLLAKGSVLTTDIINQLVR
HHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHH
LEQAEQHNLTLYTRSEEK
HHHHHCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]