Click here to switch to the map view.

The map label for this gene is hupR1 [H]

Identifier: 71906608

GI number: 71906608

Start: 1053973

End: 1054539

Strand: Reverse

Name: hupR1 [H]

Synonym: Daro_0969

Alternate gene names: 71906608

Gene position: 1054539-1053973 (Counterclockwise)

Preceding gene: 71906609

Following gene: 71906607

Centisome position: 23.43

GC content: 59.61

Gene sequence:

>567_bases
ATGAGCCGTATCATGCTCGTCGACGACGAAGAATCGATCCTCAAGTCCCTGAAACGCATCCTGCGCCTGGCCCCCTGTGC
CTTTGGCGGCAAGACCTTCCAGCTCGAAGTGGAAACCTACACCTCACCCGAAGCTGCCCTTGAAGCGGCACACCGGGACA
GCTACGACCTGTTCATCAGCGATTACCGGATGCCGATCATGGACGGCATTGAGTTCCTCAAGGCGACCAAGGAGATCCAG
CCCGATGCGGCGCGCCTGATCCTGTCCGGCTATGCCGATCTGAATGCGCTGGTCCGGGCGGTGAACGAAGTCGGCATTGA
CCGCTTCATCGGCAAACCTTGGAACGACTACGACCTGATGTCAGCCATCGGCCAAGCCCTCGCCCACCGCGAACTGCTGC
TCGAAAACCGCCAACTGGCCAACCTGGTGCGCATGGAAATGGGCGATACCACCCCGGAACAGGTCGAGGCGGAACGGCTG
GAAGCCATCGAGCCGGGCATCACCGATGTCAATTGGGGACCGGACGGTTCAGTCATTCTCGACCCAGAGTTTCAGACAAA
AAGCTGA

Upstream 100 bases:

>100_bases
CCAAGGGCAGTGTGCTGACGACGGATATCATCAATCAACTGGTCAGGCTGGAGCAGGCCGAACAGCACAACCTGACCCTC
TACACCCGCTCGGAGGAAAA

Downstream 100 bases:

>100_bases
GCGATCATGACCGAGCGTCAGGACAACAGCCTAAGCTTCAAACTGGTCTACTACGGGCCGGCGCAGAGCGGCAAGACGAC
CAATCTGCTGCGTCTGCATG

Product: response regulator receiver

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MSRIMLVDDEESILKSLKRILRLAPCAFGGKTFQLEVETYTSPEAALEAAHRDSYDLFISDYRMPIMDGIEFLKATKEIQ
PDAARLILSGYADLNALVRAVNEVGIDRFIGKPWNDYDLMSAIGQALAHRELLLENRQLANLVRMEMGDTTPEQVEAERL
EAIEPGITDVNWGPDGSVILDPEFQTKS

Sequences:

>Translated_188_residues
MSRIMLVDDEESILKSLKRILRLAPCAFGGKTFQLEVETYTSPEAALEAAHRDSYDLFISDYRMPIMDGIEFLKATKEIQ
PDAARLILSGYADLNALVRAVNEVGIDRFIGKPWNDYDLMSAIGQALAHRELLLENRQLANLVRMEMGDTTPEQVEAERL
EAIEPGITDVNWGPDGSVILDPEFQTKS
>Mature_187_residues
SRIMLVDDEESILKSLKRILRLAPCAFGGKTFQLEVETYTSPEAALEAAHRDSYDLFISDYRMPIMDGIEFLKATKEIQP
DAARLILSGYADLNALVRAVNEVGIDRFIGKPWNDYDLMSAIGQALAHRELLLENRQLANLVRMEMGDTTPEQVEAERLE
AIEPGITDVNWGPDGSVILDPEFQTKS

Specific function: Probable member of the two-component regulatory system involved in the regulation of the [NiFe] hydrogenase activity. HupR1 is probably phosphorylated by the sensory component and then acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 21087; Mature: 20956

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIMLVDDEESILKSLKRILRLAPCAFGGKTFQLEVETYTSPEAALEAAHRDSYDLFIS
CCCEEEECCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCEEEEE
DYRMPIMDGIEFLKATKEIQPDAARLILSGYADLNALVRAVNEVGIDRFIGKPWNDYDLM
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
SAIGQALAHRELLLENRQLANLVRMEMGDTTPEQVEAERLEAIEPGITDVNWGPDGSVIL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEE
DPEFQTKS
CCCCCCCC
>Mature Secondary Structure 
SRIMLVDDEESILKSLKRILRLAPCAFGGKTFQLEVETYTSPEAALEAAHRDSYDLFIS
CCEEEECCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCEEEEE
DYRMPIMDGIEFLKATKEIQPDAARLILSGYADLNALVRAVNEVGIDRFIGKPWNDYDLM
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
SAIGQALAHRELLLENRQLANLVRMEMGDTTPEQVEAERLEAIEPGITDVNWGPDGSVIL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEE
DPEFQTKS
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1885559; 8497190; 2007559 [H]