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The map label for this gene is pit [H]

Identifier: 71906318

GI number: 71906318

Start: 748061

End: 749110

Strand: Reverse

Name: pit [H]

Synonym: Daro_0679

Alternate gene names: 71906318

Gene position: 749110-748061 (Counterclockwise)

Preceding gene: 71906320

Following gene: 71906317

Centisome position: 16.64

GC content: 64.95

Gene sequence:

>1050_bases
ATGCGCATGGATACCCTGTTCCTGCTCGCTGTCGCCACCGGCGTCATTGTCCTCGTCTTCGACTACACCAACGGTTTCCA
CGATGCGGCCAATATCGTTGCCACCGTGATTGCCTCGCGCGCCATGACCCCGGCCCAGGCAGTCCTCATCGTCGGCGTCT
TCGAGTTTCTCGGCCCACTGCTCGGCGGCACCGCCGTCGCCAACACCATCGGCACGTTCGTCGCGCTCGACGGCGTGGTC
CCCATCCTGTCGCTGTCCATTCTGCTTTGCGGCCTGATCGGCGCCATTGTCTGGAATCTCGGCACCTGGTATTTCGGCAT
TCCCTCCTCGTCGTCGCACGCGCTGGTCGGCGGACTGATCGGAGCAGTCGTCGTTTCCGTCGGTAGCGAGCATGTCGTCT
GGGGTTTTGCCGAACTGGCCAATGGCCACTTGACCGGCATCGTCAAGGTATTGGCCGCGCTGCTTCTGTCGCCACTGCTC
GGCTTCTGGCTGGGCTTCCTGACTCACCGGCTACTCACCGCCCTGCTGCTTGCCGCCAACCCGGCAGCCAACGCCCGACT
ACGTGGCCTGCAATATCTCACCGCCGCCGGTCTCGCCTTCTCGCACGGCGCCAACGATGCCCAGAAGAGCATGGGTATCC
TGACGCTGGTCCTGCTCCTTGGCGGGTTCATCCCGACTTTCGAGGTTCCGCTGTGGGTCATGATCGCCTGCGCCACGTCA
ATCACGCTCGGCATCATGTCCGGTGGCTGGCGCATCGTCCGCACCCTGGGTTTCGCCATCTACCGCGTGCGGCCGATCCA
CGCCCTTGGCTCGCAACTGACCTCCTCCGCCGTGATCCTCGCCGCTTCGGTCGCTGGCGCCCCGGTATCCACCACCCATG
TCGTCGCCACCTCGATCATGGGCATCGGCGCCTCGGAACGGCCACGCGCCGTGCGCTGGGCCAAGGCCAAGGACATCGCC
ACGACCTGGCTGATCACCATTCCCGGCGCCGCCATTGTCGCCATCCAGGCCTACGCCCTGGTCCATCTCTTTCTCGGAGG
CCCGCCATGA

Upstream 100 bases:

>100_bases
ACAAGACGCACGCAAACCCTCAAAACGCCGGCAATTCGGCGATATACTGGCCCAAACAATATATACCGAAACGGAGGCTG
GGCCTGGCACTGTCGGGCCA

Downstream 100 bases:

>100_bases
GCGAAGACAACAAGCCGTCGATCTTCCGCCGCCTGTTCGATCGAGTGTTCCCGAAGATGCCGGATTTCTTCTCCCTGCTG
TCCGAACAGTGCGAGCAGGT

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MRMDTLFLLAVATGVIVLVFDYTNGFHDAANIVATVIASRAMTPAQAVLIVGVFEFLGPLLGGTAVANTIGTFVALDGVV
PILSLSILLCGLIGAIVWNLGTWYFGIPSSSSHALVGGLIGAVVVSVGSEHVVWGFAELANGHLTGIVKVLAALLLSPLL
GFWLGFLTHRLLTALLLAANPAANARLRGLQYLTAAGLAFSHGANDAQKSMGILTLVLLLGGFIPTFEVPLWVMIACATS
ITLGIMSGGWRIVRTLGFAIYRVRPIHALGSQLTSSAVILAASVAGAPVSTTHVVATSIMGIGASERPRAVRWAKAKDIA
TTWLITIPGAAIVAIQAYALVHLFLGGPP

Sequences:

>Translated_349_residues
MRMDTLFLLAVATGVIVLVFDYTNGFHDAANIVATVIASRAMTPAQAVLIVGVFEFLGPLLGGTAVANTIGTFVALDGVV
PILSLSILLCGLIGAIVWNLGTWYFGIPSSSSHALVGGLIGAVVVSVGSEHVVWGFAELANGHLTGIVKVLAALLLSPLL
GFWLGFLTHRLLTALLLAANPAANARLRGLQYLTAAGLAFSHGANDAQKSMGILTLVLLLGGFIPTFEVPLWVMIACATS
ITLGIMSGGWRIVRTLGFAIYRVRPIHALGSQLTSSAVILAASVAGAPVSTTHVVATSIMGIGASERPRAVRWAKAKDIA
TTWLITIPGAAIVAIQAYALVHLFLGGPP
>Mature_349_residues
MRMDTLFLLAVATGVIVLVFDYTNGFHDAANIVATVIASRAMTPAQAVLIVGVFEFLGPLLGGTAVANTIGTFVALDGVV
PILSLSILLCGLIGAIVWNLGTWYFGIPSSSSHALVGGLIGAVVVSVGSEHVVWGFAELANGHLTGIVKVLAALLLSPLL
GFWLGFLTHRLLTALLLAANPAANARLRGLQYLTAAGLAFSHGANDAQKSMGILTLVLLLGGFIPTFEVPLWVMIACATS
ITLGIMSGGWRIVRTLGFAIYRVRPIHALGSQLTSSAVILAASVAGAPVSTTHVVATSIMGIGASERPRAVRWAKAKDIA
TTWLITIPGAAIVAIQAYALVHLFLGGPP

Specific function: Low-affinity inorganic phosphate transport (Probable) [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=172, Percent_Identity=29.0697674418605, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI5803173, Length=160, Percent_Identity=30, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI1789907, Length=221, Percent_Identity=37.10407239819, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1789360, Length=223, Percent_Identity=35.8744394618834, Blast_Score=118, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17539280, Length=169, Percent_Identity=24.8520710059172, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI71981576, Length=148, Percent_Identity=26.3513513513513, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25146401, Length=148, Percent_Identity=25.6756756756757, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319773, Length=149, Percent_Identity=27.5167785234899, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 36113; Mature: 36113

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMDTLFLLAVATGVIVLVFDYTNGFHDAANIVATVIASRAMTPAQAVLIVGVFEFLGPL
CCCHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LGGTAVANTIGTFVALDGVVPILSLSILLCGLIGAIVWNLGTWYFGIPSSSSHALVGGLI
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHH
GAVVVSVGSEHVVWGFAELANGHLTGIVKVLAALLLSPLLGFWLGFLTHRLLTALLLAAN
HHHHHHCCCCHHEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PAANARLRGLQYLTAAGLAFSHGANDAQKSMGILTLVLLLGGFIPTFEVPLWVMIACATS
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
ITLGIMSGGWRIVRTLGFAIYRVRPIHALGSQLTSSAVILAASVAGAPVSTTHVVATSIM
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHCCCCCCHHHHHHHHHH
GIGASERPRAVRWAKAKDIATTWLITIPGAAIVAIQAYALVHLFLGGPP
CCCCCCCCCHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRMDTLFLLAVATGVIVLVFDYTNGFHDAANIVATVIASRAMTPAQAVLIVGVFEFLGPL
CCCHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LGGTAVANTIGTFVALDGVVPILSLSILLCGLIGAIVWNLGTWYFGIPSSSSHALVGGLI
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHH
GAVVVSVGSEHVVWGFAELANGHLTGIVKVLAALLLSPLLGFWLGFLTHRLLTALLLAAN
HHHHHHCCCCHHEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PAANARLRGLQYLTAAGLAFSHGANDAQKSMGILTLVLLLGGFIPTFEVPLWVMIACATS
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
ITLGIMSGGWRIVRTLGFAIYRVRPIHALGSQLTSSAVILAASVAGAPVSTTHVVATSIM
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHCCCCCCHHHHHHHHHH
GIGASERPRAVRWAKAKDIATTWLITIPGAAIVAIQAYALVHLFLGGPP
CCCCCCCCCHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9696772; 11481430 [H]