Click here to switch to the map view.

The map label for this gene is proS

Identifier: 71906284

GI number: 71906284

Start: 707027

End: 708757

Strand: Reverse

Name: proS

Synonym: Daro_0644

Alternate gene names: 71906284

Gene position: 708757-707027 (Counterclockwise)

Preceding gene: 71906288

Following gene: 71906283

Centisome position: 15.75

GC content: 58.12

Gene sequence:

>1731_bases
ATGCGCACTTCGCAGTTCTTTTTTACAACACTCAAGGAAGCCCCGGCCGACGCCGAGGTTATCAGCCAGAAGATGATGCT
GCGCGCCGGCTATATCAAGCGCGCCGCAGCCGGCATCTACACATGGATGCCACTTGGCCTGCGCGTGCTGCGCAAGGTAG
AAAACATTGTCCGCGAAGAGATGAACAACGCGGGCGCACTGGAATTATTGATGCCAGCCGTACAACCGGCCGAACTGTGG
CAAGAATCGGGGCGCTGGGAACAATATGGACCGGAATTGCTGCGTTTCAAGGATCGTCACCAGCGCGAATTCGTCATCGG
CCCAACGCATGAAGAAGTCATCACTGATGTCGTTCGCCGTGACGTGAAGAGTTATCGCCAATTGCCGATCCACCTGTATC
AAATCCAGACCAAATTCCGCGATGAAATCCGTCCCCGCTTCGGCGTCATGCGTGGTCGAGAGTTCCTGATGAAGGACGGC
TACTCGTTCCACGCCTCGTTCGACGATCTGAAGCGCGAATATGGCAACATGTACGACACCTACAGTCGTATTTTCAGCCG
TCTTGGCCTGAAATTCCGTGCGGTGGCCGCAGATACCGGTTCGATCGGCGGTACCGGTTCGCACGAATTCCATGTCCTGG
CCGATTCCGGCGAGGATGACATCGCCTTTTGCCCGGACTCCGGCTATGCCGCCAACGTCGAACTGGCCGAAGCCATGGCC
CCGAACGAGGCGCGTGCCGCCGCCTCCACGTTGATGGAAAAGACCCACACGCCGGGCAAGATGGCCTGCGCCGATGTCGC
CAAATTCCTCGAACTGCCGCTGGACAGAATCGTCAAATCGATTGCGGTCATGAGCGAGAAGGAAGATGGCAGCCAGACCT
TCGCACTGCTGCTGCTGCGTGGCGACCACGAACTGAACGAAATCAAGGCAAGCAAAATTGCCGCCATCAATCCTTTCCGC
TTCGCCACCGAAGAAGAAGTGATCGAGCGCCTCGGCTGCAAGCCAGGATTCATTGGTCCGGTTGCGATCGACGCGACGAA
GGTAGCCGTTTTTGCCGACCGTAGCGTGGCCAACATGAGCGATTTCGTCTGTGGCGCCAACGAGGCCGGCTATCACATGA
CGGGCGTCAATTTCGGCCGCGACCTGCCGGAACCGGAAGTCTTCGACATTCGCAACGTCGTCGCCGGCGACGCATCGCCG
GACGGCCAGGGTAAGCTTGAAATCCTGCGTGGCATCGAAGTCGGTCACATTTTCCAATTGCGCCAAAAATATGCTGAAGC
CTTGAACTGTGTCTATCTGGACGACAGCGGCAAGAGCCAGATCATGGAGATGGGCTGCTACGGCATTGGCGTTTCCCGCA
TCGTGGGCGCCGCTATCGAACAAGGTAACGACGACAAGGGCATCATCCTGCCGCCGGCCATTGCTCCGTTTGAAGTCTGT
ATCGTGCCAATGGGCTACCACAAGAGCGAAGCGGTCAAGGCAAGTGCCGACCAGCTTTATGCCGACCTGAAGAAGATCGG
CGTCGATGTCGTGCTCGATGACCGCAACGAGCGCCCGGGCGTCATGTTCGCCGACATGGAACTGATCGGCATCCCGCATC
GCGTGGTGATCGGCGAGCGTGGCCTGAAGGACGGGCTGTTCGAATACAAGAGCCGAATCGATGCCGAACCAACCATGGTT
GCCCAAACCGACATCGTCACCCTGCTGCAAGGGAAGCTGTGCGCCGCCTGA

Upstream 100 bases:

>100_bases
AAAATTAATAGTTACCCGGATTCTTTCACAAAGGGCGCCCACATGCAAAGAAGCCGGACCGTGTAAAATCAAGGTTTCGC
AACGAATTCAAGAGCATCCC

Downstream 100 bases:

>100_bases
TCGCGGCCCTGCTTTTTTGTGGCGCGTCGGCGGCCCACGCCGGCGCGCAGAAGTACGAGGTACTGTCGGCCAGCGCGCAG
GCCGCCCTGCACAAGGCAGT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 576; Mature: 576

Protein sequence:

>576_residues
MRTSQFFFTTLKEAPADAEVISQKMMLRAGYIKRAAAGIYTWMPLGLRVLRKVENIVREEMNNAGALELLMPAVQPAELW
QESGRWEQYGPELLRFKDRHQREFVIGPTHEEVITDVVRRDVKSYRQLPIHLYQIQTKFRDEIRPRFGVMRGREFLMKDG
YSFHASFDDLKREYGNMYDTYSRIFSRLGLKFRAVAADTGSIGGTGSHEFHVLADSGEDDIAFCPDSGYAANVELAEAMA
PNEARAAASTLMEKTHTPGKMACADVAKFLELPLDRIVKSIAVMSEKEDGSQTFALLLLRGDHELNEIKASKIAAINPFR
FATEEEVIERLGCKPGFIGPVAIDATKVAVFADRSVANMSDFVCGANEAGYHMTGVNFGRDLPEPEVFDIRNVVAGDASP
DGQGKLEILRGIEVGHIFQLRQKYAEALNCVYLDDSGKSQIMEMGCYGIGVSRIVGAAIEQGNDDKGIILPPAIAPFEVC
IVPMGYHKSEAVKASADQLYADLKKIGVDVVLDDRNERPGVMFADMELIGIPHRVVIGERGLKDGLFEYKSRIDAEPTMV
AQTDIVTLLQGKLCAA

Sequences:

>Translated_576_residues
MRTSQFFFTTLKEAPADAEVISQKMMLRAGYIKRAAAGIYTWMPLGLRVLRKVENIVREEMNNAGALELLMPAVQPAELW
QESGRWEQYGPELLRFKDRHQREFVIGPTHEEVITDVVRRDVKSYRQLPIHLYQIQTKFRDEIRPRFGVMRGREFLMKDG
YSFHASFDDLKREYGNMYDTYSRIFSRLGLKFRAVAADTGSIGGTGSHEFHVLADSGEDDIAFCPDSGYAANVELAEAMA
PNEARAAASTLMEKTHTPGKMACADVAKFLELPLDRIVKSIAVMSEKEDGSQTFALLLLRGDHELNEIKASKIAAINPFR
FATEEEVIERLGCKPGFIGPVAIDATKVAVFADRSVANMSDFVCGANEAGYHMTGVNFGRDLPEPEVFDIRNVVAGDASP
DGQGKLEILRGIEVGHIFQLRQKYAEALNCVYLDDSGKSQIMEMGCYGIGVSRIVGAAIEQGNDDKGIILPPAIAPFEVC
IVPMGYHKSEAVKASADQLYADLKKIGVDVVLDDRNERPGVMFADMELIGIPHRVVIGERGLKDGLFEYKSRIDAEPTMV
AQTDIVTLLQGKLCAA
>Mature_576_residues
MRTSQFFFTTLKEAPADAEVISQKMMLRAGYIKRAAAGIYTWMPLGLRVLRKVENIVREEMNNAGALELLMPAVQPAELW
QESGRWEQYGPELLRFKDRHQREFVIGPTHEEVITDVVRRDVKSYRQLPIHLYQIQTKFRDEIRPRFGVMRGREFLMKDG
YSFHASFDDLKREYGNMYDTYSRIFSRLGLKFRAVAADTGSIGGTGSHEFHVLADSGEDDIAFCPDSGYAANVELAEAMA
PNEARAAASTLMEKTHTPGKMACADVAKFLELPLDRIVKSIAVMSEKEDGSQTFALLLLRGDHELNEIKASKIAAINPFR
FATEEEVIERLGCKPGFIGPVAIDATKVAVFADRSVANMSDFVCGANEAGYHMTGVNFGRDLPEPEVFDIRNVVAGDASP
DGQGKLEILRGIEVGHIFQLRQKYAEALNCVYLDDSGKSQIMEMGCYGIGVSRIVGAAIEQGNDDKGIILPPAIAPFEVC
IVPMGYHKSEAVKASADQLYADLKKIGVDVVLDDRNERPGVMFADMELIGIPHRVVIGERGLKDGLFEYKSRIDAEPTMV
AQTDIVTLLQGKLCAA

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=226, Percent_Identity=46.0176991150442, Blast_Score=225, Evalue=8e-59,
Organism=Escherichia coli, GI1786392, Length=570, Percent_Identity=60.1754385964912, Blast_Score=709, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=279, Percent_Identity=36.2007168458781, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI193203271, Length=89, Percent_Identity=39.3258426966292, Blast_Score=85, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320931, Length=538, Percent_Identity=31.4126394052045, Blast_Score=256, Evalue=6e-69,
Organism=Drosophila melanogaster, GI24656200, Length=220, Percent_Identity=39.0909090909091, Blast_Score=177, Evalue=2e-44,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_DECAR (Q47ID0)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_283871.1
- ProteinModelPortal:   Q47ID0
- SMR:   Q47ID0
- STRING:   Q47ID0
- GeneID:   3568911
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_0644
- NMPDR:   fig|159087.4.peg.533
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- BioCyc:   DARO159087:DARO_0644-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 63864; Mature: 63864

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSQFFFTTLKEAPADAEVISQKMMLRAGYIKRAAAGIYTWMPLGLRVLRKVENIVREE
CCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNNAGALELLMPAVQPAELWQESGRWEQYGPELLRFKDRHQREFVIGPTHEEVITDVVRR
HCCCCCHHHHHHCCCHHHHHHHCCCCHHCCHHHHHHHHCCCCEEEECCCHHHHHHHHHHH
DVKSYRQLPIHLYQIQTKFRDEIRPRFGVMRGREFLMKDGYSFHASFDDLKREYGNMYDT
HHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHCCHHHH
YSRIFSRLGLKFRAVAADTGSIGGTGSHEFHVLADSGEDDIAFCPDSGYAANVELAEAMA
HHHHHHHHCCEEEEEEECCCCCCCCCCCEEEEEECCCCCCEEECCCCCCCCCCHHHHHCC
PNEARAAASTLMEKTHTPGKMACADVAKFLELPLDRIVKSIAVMSEKEDGSQTFALLLLR
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEEEEEE
GDHELNEIKASKIAAINPFRFATEEEVIERLGCKPGFIGPVAIDATKVAVFADRSVANMS
CCCHHHHHHHHHEEEECCCCCCCHHHHHHHHCCCCCCCCCEEECCEEEEEEECCCHHHHH
DFVCGANEAGYHMTGVNFGRDLPEPEVFDIRNVVAGDASPDGQGKLEILRGIEVGHIFQL
HHHCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHH
RQKYAEALNCVYLDDSGKSQIMEMGCYGIGVSRIVGAAIEQGNDDKGIILPPAIAPFEVC
HHHHHHHCEEEEECCCCHHHHHHHCCHHCCHHHHHHHHHHCCCCCCCEEECCCCCCEEEE
IVPMGYHKSEAVKASADQLYADLKKIGVDVVLDDRNERPGVMFADMELIGIPHRVVIGER
EEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECCCEECCCCEEEECCC
GLKDGLFEYKSRIDAEPTMVAQTDIVTLLQGKLCAA
CCHHHHHHHHHHCCCCCCEEEHHHHHHHHCCHHCCC
>Mature Secondary Structure
MRTSQFFFTTLKEAPADAEVISQKMMLRAGYIKRAAAGIYTWMPLGLRVLRKVENIVREE
CCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNNAGALELLMPAVQPAELWQESGRWEQYGPELLRFKDRHQREFVIGPTHEEVITDVVRR
HCCCCCHHHHHHCCCHHHHHHHCCCCHHCCHHHHHHHHCCCCEEEECCCHHHHHHHHHHH
DVKSYRQLPIHLYQIQTKFRDEIRPRFGVMRGREFLMKDGYSFHASFDDLKREYGNMYDT
HHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHCCHHHH
YSRIFSRLGLKFRAVAADTGSIGGTGSHEFHVLADSGEDDIAFCPDSGYAANVELAEAMA
HHHHHHHHCCEEEEEEECCCCCCCCCCCEEEEEECCCCCCEEECCCCCCCCCCHHHHHCC
PNEARAAASTLMEKTHTPGKMACADVAKFLELPLDRIVKSIAVMSEKEDGSQTFALLLLR
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEEEEEE
GDHELNEIKASKIAAINPFRFATEEEVIERLGCKPGFIGPVAIDATKVAVFADRSVANMS
CCCHHHHHHHHHEEEECCCCCCCHHHHHHHHCCCCCCCCCEEECCEEEEEEECCCHHHHH
DFVCGANEAGYHMTGVNFGRDLPEPEVFDIRNVVAGDASPDGQGKLEILRGIEVGHIFQL
HHHCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHH
RQKYAEALNCVYLDDSGKSQIMEMGCYGIGVSRIVGAAIEQGNDDKGIILPPAIAPFEVC
HHHHHHHCEEEEECCCCHHHHHHHCCHHCCHHHHHHHHHHCCCCCCCEEECCCCCCEEEE
IVPMGYHKSEAVKASADQLYADLKKIGVDVVLDDRNERPGVMFADMELIGIPHRVVIGER
EEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECCCEECCCCEEEECCC
GLKDGLFEYKSRIDAEPTMVAQTDIVTLLQGKLCAA
CCHHHHHHHHHHCCCCCCEEEHHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA