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The map label for this gene is pilB [H]

Identifier: 71906279

GI number: 71906279

Start: 701114

End: 702829

Strand: Reverse

Name: pilB [H]

Synonym: Daro_0639

Alternate gene names: 71906279

Gene position: 702829-701114 (Counterclockwise)

Preceding gene: 71906280

Following gene: 71906278

Centisome position: 15.61

GC content: 55.59

Gene sequence:

>1716_bases
ATGGCGGCAACCCCTCAAAATCCTCCACTTAGCGGCTTGGCGCGCGCGCTCGTCCAGGCCGGACATCTCAAGGAGGTAGA
GGCGGAACAATTGCTGGCCCAGGCCCACAGCACCAAGACTTCGCTGATCGAACAGATCATTACAAGCCAGAAATCCAGTG
CAATCGACATAGCGCGCTTTGTCGCCGATACCTTTGGCTATCCACTACTTGACCTCAACGCTTTCGATGAAGCCCACATT
CCGTCGGATGCCATCGACCGTAAGCTGATTGCGACGCACAAGGTCATTCCACTCAACAAGCGCGGAAACCGTTTATCGGT
AGCAATTGCTGACCCAACCAACCTTCGTGCGCTGGACGAAATTCGCTTCCAGACCGGTCTGGCTGTCGATCCGATTGTCG
TCGAACACCCAAAACTGGCGCCGCTCGTCAACAAATATGCCGAGACAGCCGCCGAAGCGCTGAAAAACTTCACCAGCGAG
GATCTCAACCTCGATTTTCTGGACGAAGAAACCTCCAGCAAAGCTGACGAAGCCGCAGGGCAGGAAATCGATGACGCACC
GGTCGTCAAATTCATCCAAAAAATGCTGCTCGATGCCATCAATGATGGAGCATCGGACATCCATTTCGAACCATACGAAA
AGTTTTATCGCATTCGCTTCCGCGTCGACGGCATCCTGCGCGAAGTAGCCACTCCACCGCTGGCCATCAAGGAAAAAATT
GCCTCGCGCATCAAGGTCATTTCCCGGCTTAATATCGCCGAAAAGCGCGTCCCGCAGGACGGCCGGATGAAACTGGTGCT
TTCGAAGACCCGTGCCATCGATTTCCGGGTCAGCACACTACCGACGCTTCAGGGCGAGAAAATCGTTATGCGTATTCTCG
ACCCAAGCTCAGCCACCTTGGGCATCGAGGCGCTAGGCTACGAGCCGGAGCAAAAAGCAGCAATAATGGACGCCATCAGC
CGCCCCTATGGGATGGTACTGGTCACCGGACCGACAGGTTCTGGCAAAACCGTTTCGCTCTACACCTGCCTCAACATCCT
GAATAAGGATGGCATCAACATTTCGACAGCGGAAGACCCGGCTGAAATCAATCTACCGGGTGTCAATCAAGTCAACGTCG
ACGACCGTGCTGGCCTGACCTTCCCTGTCGCACTAAAGGCATTCCTGCGCCAGGATCCGGACATCATCATGGTCGGCGAA
ATTCGTGACCTGGAGACCGCCGAGATTTCCATCAAGGCGGCACAAACGGGTCACCTGGTGCTCTCGACGCTGCACACCAA
CGATGCCCCGCAAACACTGACTCGACTGATGAACATGGGCGTCCCCATGTTCAATATCGCCTCCAGCGTGCTGTTGATCA
CCGCTCAGCGCTTGGCGCGACGGCTATGCAACTGCAAGAAACCGATGACCGTACCCGACCAGGCACTACTGGATGCAGGC
TACTCGGAAGCCGATCTCGACGGTTCGTGGACGCTGTTTGGCCCAGGAGGATGTGAACGGTGCAAAGGGACCGGCTACAA
GGGACGGGTCGGCATTTATCAGGTCATGCCCATTTCCGAAGCTATGCAACGCATGATCATGAGCGGCGCATCTGCACTGG
ACTTGGGCGCCCAAGCGAAAGCCGAAGGCGTGAAAAACCTCCGCGAATCCGGACTATTAAAAGTCAAACAAGGTGTGACC
TCGCTTGACGAGGTGCTCAGCACCACCAACGCTTAA

Upstream 100 bases:

>100_bases
TCCGCCCCGAAGCTGGCTGAGCAAAACCCGCCCCCAGCCTTTACAAACAATCGCCTACGTAGCATCATCAAGCGATCTAA
ACGGCATTTAACATCGATCA

Downstream 100 bases:

>100_bases
CGAAAAGGAACTTGTATGGCTACCGCCGCAAGACGTAAGGCCTCGGCAGTCAAGGAAAGCACCTTCCTCTGGGAAGGCAA
GAACAAGGATGGCAAGACCG

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 571; Mature: 570

Protein sequence:

>571_residues
MAATPQNPPLSGLARALVQAGHLKEVEAEQLLAQAHSTKTSLIEQIITSQKSSAIDIARFVADTFGYPLLDLNAFDEAHI
PSDAIDRKLIATHKVIPLNKRGNRLSVAIADPTNLRALDEIRFQTGLAVDPIVVEHPKLAPLVNKYAETAAEALKNFTSE
DLNLDFLDEETSSKADEAAGQEIDDAPVVKFIQKMLLDAINDGASDIHFEPYEKFYRIRFRVDGILREVATPPLAIKEKI
ASRIKVISRLNIAEKRVPQDGRMKLVLSKTRAIDFRVSTLPTLQGEKIVMRILDPSSATLGIEALGYEPEQKAAIMDAIS
RPYGMVLVTGPTGSGKTVSLYTCLNILNKDGINISTAEDPAEINLPGVNQVNVDDRAGLTFPVALKAFLRQDPDIIMVGE
IRDLETAEISIKAAQTGHLVLSTLHTNDAPQTLTRLMNMGVPMFNIASSVLLITAQRLARRLCNCKKPMTVPDQALLDAG
YSEADLDGSWTLFGPGGCERCKGTGYKGRVGIYQVMPISEAMQRMIMSGASALDLGAQAKAEGVKNLRESGLLKVKQGVT
SLDEVLSTTNA

Sequences:

>Translated_571_residues
MAATPQNPPLSGLARALVQAGHLKEVEAEQLLAQAHSTKTSLIEQIITSQKSSAIDIARFVADTFGYPLLDLNAFDEAHI
PSDAIDRKLIATHKVIPLNKRGNRLSVAIADPTNLRALDEIRFQTGLAVDPIVVEHPKLAPLVNKYAETAAEALKNFTSE
DLNLDFLDEETSSKADEAAGQEIDDAPVVKFIQKMLLDAINDGASDIHFEPYEKFYRIRFRVDGILREVATPPLAIKEKI
ASRIKVISRLNIAEKRVPQDGRMKLVLSKTRAIDFRVSTLPTLQGEKIVMRILDPSSATLGIEALGYEPEQKAAIMDAIS
RPYGMVLVTGPTGSGKTVSLYTCLNILNKDGINISTAEDPAEINLPGVNQVNVDDRAGLTFPVALKAFLRQDPDIIMVGE
IRDLETAEISIKAAQTGHLVLSTLHTNDAPQTLTRLMNMGVPMFNIASSVLLITAQRLARRLCNCKKPMTVPDQALLDAG
YSEADLDGSWTLFGPGGCERCKGTGYKGRVGIYQVMPISEAMQRMIMSGASALDLGAQAKAEGVKNLRESGLLKVKQGVT
SLDEVLSTTNA
>Mature_570_residues
AATPQNPPLSGLARALVQAGHLKEVEAEQLLAQAHSTKTSLIEQIITSQKSSAIDIARFVADTFGYPLLDLNAFDEAHIP
SDAIDRKLIATHKVIPLNKRGNRLSVAIADPTNLRALDEIRFQTGLAVDPIVVEHPKLAPLVNKYAETAAEALKNFTSED
LNLDFLDEETSSKADEAAGQEIDDAPVVKFIQKMLLDAINDGASDIHFEPYEKFYRIRFRVDGILREVATPPLAIKEKIA
SRIKVISRLNIAEKRVPQDGRMKLVLSKTRAIDFRVSTLPTLQGEKIVMRILDPSSATLGIEALGYEPEQKAAIMDAISR
PYGMVLVTGPTGSGKTVSLYTCLNILNKDGINISTAEDPAEINLPGVNQVNVDDRAGLTFPVALKAFLRQDPDIIMVGEI
RDLETAEISIKAAQTGHLVLSTLHTNDAPQTLTRLMNMGVPMFNIASSVLLITAQRLARRLCNCKKPMTVPDQALLDAGY
SEADLDGSWTLFGPGGCERCKGTGYKGRVGIYQVMPISEAMQRMIMSGASALDLGAQAKAEGVKNLRESGLLKVKQGVTS
LDEVLSTTNA

Specific function: Involved in the translocation of the type IV pilin (pilA) [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=413, Percent_Identity=44.5520581113801, Blast_Score=332, Evalue=4e-92,
Organism=Escherichia coli, GI1786296, Length=385, Percent_Identity=43.1168831168831, Blast_Score=319, Evalue=4e-88,
Organism=Escherichia coli, GI87082188, Length=246, Percent_Identity=30.0813008130081, Blast_Score=100, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 61955; Mature: 61824

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00046 HISTONE_H2A ; PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATPQNPPLSGLARALVQAGHLKEVEAEQLLAQAHSTKTSLIEQIITSQKSSAIDIARF
CCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
VADTFGYPLLDLNAFDEAHIPSDAIDRKLIATHKVIPLNKRGNRLSVAIADPTNLRALDE
HHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCEEEEEEECCCCCHHHHH
IRFQTGLAVDPIVVEHPKLAPLVNKYAETAAEALKNFTSEDLNLDFLDEETSSKADEAAG
HHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHCC
QEIDDAPVVKFIQKMLLDAINDGASDIHFEPYEKFYRIRFRVDGILREVATPPLAIKEKI
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ASRIKVISRLNIAEKRVPQDGRMKLVLSKTRAIDFRVSTLPTLQGEKIVMRILDPSSATL
HHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEEECCCCCCCCEEEEEEECCCCCEE
GIEALGYEPEQKAAIMDAISRPYGMVLVTGPTGSGKTVSLYTCLNILNKDGINISTAEDP
EEHCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCEEEHHHHHHHHCCCCCEEECCCCC
AEINLPGVNQVNVDDRAGLTFPVALKAFLRQDPDIIMVGEIRDLETAEISIKAAQTGHLV
CEECCCCCCEECCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEEEECCCCEE
LSTLHTNDAPQTLTRLMNMGVPMFNIASSVLLITAQRLARRLCNCKKPMTVPDQALLDAG
EEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCC
YSEADLDGSWTLFGPGGCERCKGTGYKGRVGIYQVMPISEAMQRMIMSGASALDLGAQAK
CCCCCCCCCEEEECCCCCHHCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCHHCCCCHHH
AEGVKNLRESGLLKVKQGVTSLDEVLSTTNA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AATPQNPPLSGLARALVQAGHLKEVEAEQLLAQAHSTKTSLIEQIITSQKSSAIDIARF
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
VADTFGYPLLDLNAFDEAHIPSDAIDRKLIATHKVIPLNKRGNRLSVAIADPTNLRALDE
HHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCEEEEEEECCCCCHHHHH
IRFQTGLAVDPIVVEHPKLAPLVNKYAETAAEALKNFTSEDLNLDFLDEETSSKADEAAG
HHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHCC
QEIDDAPVVKFIQKMLLDAINDGASDIHFEPYEKFYRIRFRVDGILREVATPPLAIKEKI
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ASRIKVISRLNIAEKRVPQDGRMKLVLSKTRAIDFRVSTLPTLQGEKIVMRILDPSSATL
HHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEEECCCCCCCCEEEEEEECCCCCEE
GIEALGYEPEQKAAIMDAISRPYGMVLVTGPTGSGKTVSLYTCLNILNKDGINISTAEDP
EEHCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCEEEHHHHHHHHCCCCCEEECCCCC
AEINLPGVNQVNVDDRAGLTFPVALKAFLRQDPDIIMVGEIRDLETAEISIKAAQTGHLV
CEECCCCCCEECCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEEEECCCCEE
LSTLHTNDAPQTLTRLMNMGVPMFNIASSVLLITAQRLARRLCNCKKPMTVPDQALLDAG
EEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCC
YSEADLDGSWTLFGPGGCERCKGTGYKGRVGIYQVMPISEAMQRMIMSGASALDLGAQAK
CCCCCCCCCEEEECCCCCHHCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCHHCCCCHHH
AEGVKNLRESGLLKVKQGVTSLDEVLSTTNA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1971619; 10984043 [H]