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The map label for this gene is pilD [H]

Identifier: 71906277

GI number: 71906277

Start: 699018

End: 699872

Strand: Reverse

Name: pilD [H]

Synonym: Daro_0637

Alternate gene names: 71906277

Gene position: 699872-699018 (Counterclockwise)

Preceding gene: 71906278

Following gene: 71906276

Centisome position: 15.55

GC content: 57.54

Gene sequence:

>855_bases
ATGCTTCCTGAAACGCAGGGCGGCCTGGCTGCCATAGCTGGGCTACTTGGTTTGTGCGTCGGTAGTTTCCTCAACGTCGT
GATCCACCGACTACCCAAGATGATGGAGCGGGAGTGGCTCGGACAATGCGCGGAATTGCGGGGCGAAACCGCACCTGCCT
CCCTTGAAGCATTGTCGTTGGCAAAACCACGCTCCCGCTGCCCTGACTGTGGCCACCAAATAACAGCACTGGAAAACATT
CCGCTGCTAAGTTATCTATTTATCCTGAAGGGAAAATGCTCCGGGTGCGGATGCCGGATATCTCCGCGTTACCCGATCGT
CGAGGCGATTACCGGACTACTGTCTGCTTACGTTGCCTGGCACTTTGGCCCGACTACGCAGACAATCGGCGCTCTGCTGC
TGCTCTGGGCGCTGATCTCACTTTCTGCCATTGACCTGGACACCCAGCTCCTTCCTGACTCAATCACCCTGCCTTTGCTC
TGGCTGGGACTACTCTTCAACCTCTGGAACACTTATACCGACCTCCCGTCGGCCGTCATCGGCGCCGTCGCCGGCTACCT
AACCCTTTGGAGCGTCTTTTGGCTATTCAAGCTAGCCACGGGCAAGGAAGGCATGGGCTATGGCGACTTCAAGCTCCTTG
CTGCACTGGGGGCATGGCTAGGCTGGCAAATGTTGCCGGCGATCATCCTGCTCTCGTCTGTTGTTGGTGCCGTTGTCGGT
GTCACGCTGATCGTTGTCGCCCGTCACGGCCGCAACATTCCTATTCCTTTCGGCCCTTATCTGGCGGCAGCCGGAGGAAT
TGCCCTTTTCTGGGGCGCGCAGATCACGAACACTTACCTTGGCATGATCGCTTGA

Upstream 100 bases:

>100_bases
GCCTGATGGAACCAATCATCATGGTCGTGCTCGGCACCTTGATCGGCGGCATGGTCGTCGCCATGTACCTGCCCATCTTC
AAACTGGGATCGGTGGTCTG

Downstream 100 bases:

>100_bases
AATAACGCAAGTCGACCCAATATTGGGTATTCAGACTCTCGGTTATAATTCAAGGTTTTGGAGGACTCCCATGCCGATTT
ACGAATATCGCTGTGATTCC

Product: type 4 prepilin peptidase 1. Aspartic peptidase. MEROPS family A24A

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MLPETQGGLAAIAGLLGLCVGSFLNVVIHRLPKMMEREWLGQCAELRGETAPASLEALSLAKPRSRCPDCGHQITALENI
PLLSYLFILKGKCSGCGCRISPRYPIVEAITGLLSAYVAWHFGPTTQTIGALLLLWALISLSAIDLDTQLLPDSITLPLL
WLGLLFNLWNTYTDLPSAVIGAVAGYLTLWSVFWLFKLATGKEGMGYGDFKLLAALGAWLGWQMLPAIILLSSVVGAVVG
VTLIVVARHGRNIPIPFGPYLAAAGGIALFWGAQITNTYLGMIA

Sequences:

>Translated_284_residues
MLPETQGGLAAIAGLLGLCVGSFLNVVIHRLPKMMEREWLGQCAELRGETAPASLEALSLAKPRSRCPDCGHQITALENI
PLLSYLFILKGKCSGCGCRISPRYPIVEAITGLLSAYVAWHFGPTTQTIGALLLLWALISLSAIDLDTQLLPDSITLPLL
WLGLLFNLWNTYTDLPSAVIGAVAGYLTLWSVFWLFKLATGKEGMGYGDFKLLAALGAWLGWQMLPAIILLSSVVGAVVG
VTLIVVARHGRNIPIPFGPYLAAAGGIALFWGAQITNTYLGMIA
>Mature_284_residues
MLPETQGGLAAIAGLLGLCVGSFLNVVIHRLPKMMEREWLGQCAELRGETAPASLEALSLAKPRSRCPDCGHQITALENI
PLLSYLFILKGKCSGCGCRISPRYPIVEAITGLLSAYVAWHFGPTTQTIGALLLLWALISLSAIDLDTQLLPDSITLPLL
WLGLLFNLWNTYTDLPSAVIGAVAGYLTLWSVFWLFKLATGKEGMGYGDFKLLAALGAWLGWQMLPAIILLSSVVGAVVG
VTLIVVARHGRNIPIPFGPYLAAAGGIALFWGAQITNTYLGMIA

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=254, Percent_Identity=40.5511811023622, Blast_Score=151, Evalue=5e-38,
Organism=Escherichia coli, GI1789732, Length=142, Percent_Identity=53.5211267605634, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 30389; Mature: 30389

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPETQGGLAAIAGLLGLCVGSFLNVVIHRLPKMMEREWLGQCAELRGETAPASLEALSL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
AKPRSRCPDCGHQITALENIPLLSYLFILKGKCSGCGCRISPRYPIVEAITGLLSAYVAW
CCCHHCCCCCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
HFGPTTQTIGALLLLWALISLSAIDLDTQLLPDSITLPLLWLGLLFNLWNTYTDLPSAVI
HCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAVAGYLTLWSVFWLFKLATGKEGMGYGDFKLLAALGAWLGWQMLPAIILLSSVVGAVVG
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTLIVVARHGRNIPIPFGPYLAAAGGIALFWGAQITNTYLGMIA
HHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLPETQGGLAAIAGLLGLCVGSFLNVVIHRLPKMMEREWLGQCAELRGETAPASLEALSL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
AKPRSRCPDCGHQITALENIPLLSYLFILKGKCSGCGCRISPRYPIVEAITGLLSAYVAW
CCCHHCCCCCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
HFGPTTQTIGALLLLWALISLSAIDLDTQLLPDSITLPLLWLGLLFNLWNTYTDLPSAVI
HCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAVAGYLTLWSVFWLFKLATGKEGMGYGDFKLLAALGAWLGWQMLPAIILLSSVVGAVVG
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTLIVVARHGRNIPIPFGPYLAAAGGIALFWGAQITNTYLGMIA
HHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA