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The map label for this gene is moeB [H]

Identifier: 71906246

GI number: 71906246

Start: 676162

End: 676923

Strand: Reverse

Name: moeB [H]

Synonym: Daro_0606

Alternate gene names: 71906246

Gene position: 676923-676162 (Counterclockwise)

Preceding gene: 71906247

Following gene: 71906241

Centisome position: 15.04

GC content: 62.86

Gene sequence:

>762_bases
ATGACTGACGACCAGCTACTCCGCTATAGCCGCCATATCCTGCTCGACGCCCTGGGCATCGACGGCCAGGCAAAAATCCT
GGCCTCACACGCGCTAATCATCGGCGCCGGCGGCCTTGGCTCGCCAGCCGCGCTGTACCTCGCCTCGGCAGGCATTGGCA
AGATCACGCTCGTCGATGACGACACCGTCGATTTCACCAACCTGCAGCGGCAGATCCTGCATACGCAGAGTCGCGTCGGC
ATGGCCAAAACCGAATCGGGCCGACAGGCACTGAGCGCCATCAACCCGGAAATTTCCATCGTTCCGCTGCAACAGCGTCT
ATCCGGTACCGAGCTTGACGCTCTGGTCGCCAGCGCCGATATCGTGCTCGATTGCACCGACAATTTCGCGACCCGCCACG
CCATCAATCGCGCCTGCGTCCTTCACAAAAAATCGCTGATTTCCGGCGCCGCCATCCGTTTCGACGGCCAGATCAGCGTC
TACGACCTGCGCCGCGACGATTCGCCGTGCTACCACTGCCTGTTTCCGGAGGGCGAGGATGTCGAGGAAGTGCGCTGTGC
AGTCATGGGGGTTTTCGCCCCGCTGACCGGCATCATCGGCACCATGCAGGCAGCGGAAGCCCTCAAGCTGGCCGCCGGCA
TCGGCGAATCACTGACCGGCCGGCTGCTGCTACTCGACGCCTTAACGATGGAATGGCGAACCGTCCGCTTCAAGAAGGAC
AGCGGCTGCGCCGTGTGCGGGCCGAATGCTGACGCGCACTGA

Upstream 100 bases:

>100_bases
CACGCCGCTGGAGTGGCGCGAATACAAGTAGTTAACAGGATCGAGGATCGAGCTGTCAGCAAGACCGCTCGCCCCTAATC
ACCCGATCCCCGATCCTAAA

Downstream 100 bases:

>100_bases
TTGAGATGCGAAGACTGGCCGCAGGCAGGCGTCAGCGCGCGTCAGAATTGCCCAGGCGGGCAAGCACCCGAATATCTGCC
CCGACCTGACGCAGGTCACG

Product: UBA/THIF-type NAD/FAD binding fold:MoeZ/MoeB

Products: NA

Alternate protein names: MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MTDDQLLRYSRHILLDALGIDGQAKILASHALIIGAGGLGSPAALYLASAGIGKITLVDDDTVDFTNLQRQILHTQSRVG
MAKTESGRQALSAINPEISIVPLQQRLSGTELDALVASADIVLDCTDNFATRHAINRACVLHKKSLISGAAIRFDGQISV
YDLRRDDSPCYHCLFPEGEDVEEVRCAVMGVFAPLTGIIGTMQAAEALKLAAGIGESLTGRLLLLDALTMEWRTVRFKKD
SGCAVCGPNADAH

Sequences:

>Translated_253_residues
MTDDQLLRYSRHILLDALGIDGQAKILASHALIIGAGGLGSPAALYLASAGIGKITLVDDDTVDFTNLQRQILHTQSRVG
MAKTESGRQALSAINPEISIVPLQQRLSGTELDALVASADIVLDCTDNFATRHAINRACVLHKKSLISGAAIRFDGQISV
YDLRRDDSPCYHCLFPEGEDVEEVRCAVMGVFAPLTGIIGTMQAAEALKLAAGIGESLTGRLLLLDALTMEWRTVRFKKD
SGCAVCGPNADAH
>Mature_252_residues
TDDQLLRYSRHILLDALGIDGQAKILASHALIIGAGGLGSPAALYLASAGIGKITLVDDDTVDFTNLQRQILHTQSRVGM
AKTESGRQALSAINPEISIVPLQQRLSGTELDALVASADIVLDCTDNFATRHAINRACVLHKKSLISGAAIRFDGQISVY
DLRRDDSPCYHCLFPEGEDVEEVRCAVMGVFAPLTGIIGTMQAAEALKLAAGIGESLTGRLLLLDALTMEWRTVRFKKDS
GCAVCGPNADAH

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein moaD [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=250, Percent_Identity=45.6, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI4885649, Length=143, Percent_Identity=27.972027972028, Blast_Score=72, Evalue=4e-13,
Organism=Escherichia coli, GI1787048, Length=250, Percent_Identity=47.2, Blast_Score=237, Evalue=6e-64,
Organism=Escherichia coli, GI87082356, Length=252, Percent_Identity=48.8095238095238, Blast_Score=220, Evalue=6e-59,
Organism=Escherichia coli, GI1789177, Length=141, Percent_Identity=34.0425531914894, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17540406, Length=260, Percent_Identity=41.9230769230769, Blast_Score=203, Evalue=7e-53,
Organism=Caenorhabditis elegans, GI193203301, Length=155, Percent_Identity=31.6129032258064, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6321903, Length=252, Percent_Identity=35.7142857142857, Blast_Score=168, Evalue=9e-43,
Organism=Saccharomyces cerevisiae, GI6322639, Length=175, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6320598, Length=162, Percent_Identity=28.3950617283951, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24582879, Length=248, Percent_Identity=43.9516129032258, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24660640, Length=152, Percent_Identity=30.2631578947368, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24641311, Length=258, Percent_Identity=30.2325581395349, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR012730
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.-

Molecular weight: Translated: 26997; Mature: 26866

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDDQLLRYSRHILLDALGIDGQAKILASHALIIGAGGLGSPAALYLASAGIGKITLVDD
CCHHHHHHHHHHHHEEEECCCCCHHEEEEEEEEEECCCCCCCHHHEEEECCCCEEEEEEC
DTVDFTNLQRQILHTQSRVGMAKTESGRQALSAINPEISIVPLQQRLSGTELDALVASAD
CCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEEHHHHCCCCHHHHHHCCCC
IVLDCTDNFATRHAINRACVLHKKSLISGAAIRFDGQISVYDLRRDDSPCYHCLFPEGED
EEEECCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCEEEEEEECCCCCCEEEEECCCCCC
VEEVRCAVMGVFAPLTGIIGTMQAAEALKLAAGIGESLTGRLLLLDALTMEWRTVRFKKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHCCEEEEEEECC
SGCAVCGPNADAH
CCCEEECCCCCCC
>Mature Secondary Structure 
TDDQLLRYSRHILLDALGIDGQAKILASHALIIGAGGLGSPAALYLASAGIGKITLVDD
CHHHHHHHHHHHHEEEECCCCCHHEEEEEEEEEECCCCCCCHHHEEEECCCCEEEEEEC
DTVDFTNLQRQILHTQSRVGMAKTESGRQALSAINPEISIVPLQQRLSGTELDALVASAD
CCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEEHHHHCCCCHHHHHHCCCC
IVLDCTDNFATRHAINRACVLHKKSLISGAAIRFDGQISVYDLRRDDSPCYHCLFPEGED
EEEECCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCEEEEEEECCCCCCEEEEECCCCCC
VEEVRCAVMGVFAPLTGIIGTMQAAEALKLAAGIGESLTGRLLLLDALTMEWRTVRFKKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHCCEEEEEEECC
SGCAVCGPNADAH
CCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3045084; 8905232; 9278503 [H]