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The map label for this gene is thyA

Identifier: 71906233

GI number: 71906233

Start: 663441

End: 664235

Strand: Reverse

Name: thyA

Synonym: Daro_0593

Alternate gene names: 71906233

Gene position: 664235-663441 (Counterclockwise)

Preceding gene: 71906235

Following gene: 71906232

Centisome position: 14.76

GC content: 60.13

Gene sequence:

>795_bases
ATGCAGCCCTACCTCGACCTGATGCGCCACGTGCTGGACAACGGCAATGACAAATCCGACCGTACCGGCACTGGCACGCG
CTCCGTCTTCGGCTGGCAGATGCGCTTTGACCTGAGCCAGGGCTTCCCGGTGATGACGACCAAGAAGCTGCACCTGAAAT
CGATCATCCACGAACTGCTGTGGTTCATCCAGGGCGACACCAACATCAAGTACCTGCAGGAAAACGGCGTCAAGATCTGG
GACGAATGGGCCGATGAAAACGGCGATCTCGGCCCGGTCTACGGCAAGCAGTGGCGCCGCTGGGAAACGCCGGACGGCCG
CGTCATCGACCAGATCACGCAACTGGTCAACAGCCTGAAGAACAATCCGGATTCCCGCCGCCACATCGTCTCCGCCTGGA
ACCCCGGCGATGTGGACAACATGGCCCTGCCACCCTGCCACTGCCTTTTCCAGTTTTACGTCGCAAACGGCAAGCTGTCC
TGCCAGCTCTACCAGCGCAGCGCCGACATTTTCCTCGGCGTGCCATTCAACATCGCCTCCTATGCCCTGTTCACGATGAT
GCTGGCGCAGGTTTGCGGCTACCAGCCAGGCGAATTCGTCCATACCTTTGGCGACGCTCATCTTTACTCGAACCACTTCG
AGCAGGCAAAACTACAGCTTTCCCGCGACACCCGCCCCCTGCCAACCATGTGGATCAATCCGGAAGTGAAGGACCTGTTC
GCCTTCCGCTTCGAGGATTTCCGCCTCGAAGGCTACGACCCGCACCCGCACATCCCGGCCCCGGTGGCCGTTTAA

Upstream 100 bases:

>100_bases
CTATTTCCTGATCGCCCCAATCGCTACGGCTCCCGCCCCATGGGCCTCGGTCCACCATGTAAAATAAGACGATTAATTGA
TCGCCTGAAAGACCGACCCG

Downstream 100 bases:

>100_bases
GCAATGCTGCTCGGCGGCGATCTCGGCGGCACCAAGACGCTGCTGGCACTGGCCGAAGTGATTGATGGCCGCATCGAGAT
TGTCCGCCAGCAACGCTACG

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MQPYLDLMRHVLDNGNDKSDRTGTGTRSVFGWQMRFDLSQGFPVMTTKKLHLKSIIHELLWFIQGDTNIKYLQENGVKIW
DEWADENGDLGPVYGKQWRRWETPDGRVIDQITQLVNSLKNNPDSRRHIVSAWNPGDVDNMALPPCHCLFQFYVANGKLS
CQLYQRSADIFLGVPFNIASYALFTMMLAQVCGYQPGEFVHTFGDAHLYSNHFEQAKLQLSRDTRPLPTMWINPEVKDLF
AFRFEDFRLEGYDPHPHIPAPVAV

Sequences:

>Translated_264_residues
MQPYLDLMRHVLDNGNDKSDRTGTGTRSVFGWQMRFDLSQGFPVMTTKKLHLKSIIHELLWFIQGDTNIKYLQENGVKIW
DEWADENGDLGPVYGKQWRRWETPDGRVIDQITQLVNSLKNNPDSRRHIVSAWNPGDVDNMALPPCHCLFQFYVANGKLS
CQLYQRSADIFLGVPFNIASYALFTMMLAQVCGYQPGEFVHTFGDAHLYSNHFEQAKLQLSRDTRPLPTMWINPEVKDLF
AFRFEDFRLEGYDPHPHIPAPVAV
>Mature_264_residues
MQPYLDLMRHVLDNGNDKSDRTGTGTRSVFGWQMRFDLSQGFPVMTTKKLHLKSIIHELLWFIQGDTNIKYLQENGVKIW
DEWADENGDLGPVYGKQWRRWETPDGRVIDQITQLVNSLKNNPDSRRHIVSAWNPGDVDNMALPPCHCLFQFYVANGKLS
CQLYQRSADIFLGVPFNIASYALFTMMLAQVCGYQPGEFVHTFGDAHLYSNHFEQAKLQLSRDTRPLPTMWINPEVKDLF
AFRFEDFRLEGYDPHPHIPAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=281, Percent_Identity=51.2455516014235, Blast_Score=292, Evalue=2e-79,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=69.3181818181818, Blast_Score=392, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI71993377, Length=282, Percent_Identity=46.8085106382979, Blast_Score=266, Evalue=7e-72,
Organism=Saccharomyces cerevisiae, GI6324648, Length=292, Percent_Identity=42.8082191780822, Blast_Score=215, Evalue=7e-57,
Organism=Drosophila melanogaster, GI17137556, Length=283, Percent_Identity=53.356890459364, Blast_Score=301, Evalue=4e-82,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_DECAR (Q47II1)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_283820.1
- HSSP:   P0A884
- ProteinModelPortal:   Q47II1
- SMR:   Q47II1
- STRING:   Q47II1
- GeneID:   3568761
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_0593
- NMPDR:   fig|159087.4.peg.442
- eggNOG:   COG0207
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- ProtClustDB:   PRK01827
- BioCyc:   DARO159087:DARO_0593-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 30507; Mature: 30507

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 146-146

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPYLDLMRHVLDNGNDKSDRTGTGTRSVFGWQMRFDLSQGFPVMTTKKLHLKSIIHELL
CCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCCCEEECHHHHHHHHHHHHH
WFIQGDTNIKYLQENGVKIWDEWADENGDLGPVYGKQWRRWETPDGRVIDQITQLVNSLK
HHHCCCCCEEEEECCCCEEEEHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
NNPDSRRHIVSAWNPGDVDNMALPPCHCLFQFYVANGKLSCQLYQRSADIFLGVPFNIAS
CCCCCHHEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCEEEECCHHHHH
YALFTMMLAQVCGYQPGEFVHTFGDAHLYSNHFEQAKLQLSRDTRPLPTMWINPEVKDLF
HHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHH
AFRFEDFRLEGYDPHPHIPAPVAV
HHHHHHEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MQPYLDLMRHVLDNGNDKSDRTGTGTRSVFGWQMRFDLSQGFPVMTTKKLHLKSIIHELL
CCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCCCEEECHHHHHHHHHHHHH
WFIQGDTNIKYLQENGVKIWDEWADENGDLGPVYGKQWRRWETPDGRVIDQITQLVNSLK
HHHCCCCCEEEEECCCCEEEEHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
NNPDSRRHIVSAWNPGDVDNMALPPCHCLFQFYVANGKLSCQLYQRSADIFLGVPFNIAS
CCCCCHHEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCEEEECCHHHHH
YALFTMMLAQVCGYQPGEFVHTFGDAHLYSNHFEQAKLQLSRDTRPLPTMWINPEVKDLF
HHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHH
AFRFEDFRLEGYDPHPHIPAPVAV
HHHHHHEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA