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The map label for this gene is echA8 [H]

Identifier: 71906198

GI number: 71906198

Start: 618970

End: 619758

Strand: Reverse

Name: echA8 [H]

Synonym: Daro_0558

Alternate gene names: 71906198

Gene position: 619758-618970 (Counterclockwise)

Preceding gene: 71906201

Following gene: 71906197

Centisome position: 13.77

GC content: 62.61

Gene sequence:

>789_bases
ATGACCCAGGAAGAACCCCTCGTTTTACGTCAGGACCGGGCCGATGGCCTGACCACGCTGACCCTGAACCGTCCGGGGCA
GTTCAATTCGCTGTCCAAGGACATGCTGACCGCCATCCAGGCCGAACTGGATGCCATCGCTGCCTCCGATAGCGTGCGCG
TTGTTGTCATCGCCGGTGCCGGCAAGGCCTTCTGCGCCGGGCATGACCTCAAGGAAATGCGCGGCAACCACAGCAAGGCT
TTCATGCAGGCGCTGTTCAAACAGTGCGGCAAGCTGATGCTGACGATCACCCAAATGCCGCAGCCGGTGATCGCCCGCGT
GCACGGCATCGCCACGGCGGCTGGCTGCCAGCTGGTCTCGATGTGCGATCTGGCGGTAGCCGCTGATGTGTCGAAATTCG
CCGTTTCCGGCATCAATGTCGGTCTCTTCTGCTCGACCCCGGCCGTCGGCCTGGCCCGCAACCTCGGCCGCAAGGCAGCG
CTGGAGATGCTGCTTACCGGCGAATTCATCGACGCCATGGAAGCCAAGGCCAAGGGCCTGGTCAATCGCGTCGTTCCGGC
CGATGCACTGGATGCAGAAATCGAACGTCTGGCTGGCAGCATCCTGGCCAAGAGCGCGGTCGCCGTGCGCATGGGCAAGG
GCATGTTCTACCAGCAGCTGGAAATGGGCCTGAGCGAAGCCTACGAGTACGCCAGCGAAGTGATGGCCTGCAACATGATG
AGCGATGACGCCGGCGAAGGCATCGACGCCTTCATGCAGAAGCGCAAGCCGGTTTATACCGGGCGCTAG

Upstream 100 bases:

>100_bases
TCCTTGCGATTTTACCGCGACCGGATATGGGCAGCCGATTACATTTCGTCGACGCATCGGCTAGATTAGCGGTTTCAAAT
AAAACAGCCGGAGACAAGCC

Downstream 100 bases:

>100_bases
ACGTCTTCGCTGAACCCGGCCTGTTGCGTCATCAGCATTCGGCCGGCTTCACATTCGCGGCTAGCCGCAATACCACCTTG
AAAGCCGGCGTCACGGCCGG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MTQEEPLVLRQDRADGLTTLTLNRPGQFNSLSKDMLTAIQAELDAIAASDSVRVVVIAGAGKAFCAGHDLKEMRGNHSKA
FMQALFKQCGKLMLTITQMPQPVIARVHGIATAAGCQLVSMCDLAVAADVSKFAVSGINVGLFCSTPAVGLARNLGRKAA
LEMLLTGEFIDAMEAKAKGLVNRVVPADALDAEIERLAGSILAKSAVAVRMGKGMFYQQLEMGLSEAYEYASEVMACNMM
SDDAGEGIDAFMQKRKPVYTGR

Sequences:

>Translated_262_residues
MTQEEPLVLRQDRADGLTTLTLNRPGQFNSLSKDMLTAIQAELDAIAASDSVRVVVIAGAGKAFCAGHDLKEMRGNHSKA
FMQALFKQCGKLMLTITQMPQPVIARVHGIATAAGCQLVSMCDLAVAADVSKFAVSGINVGLFCSTPAVGLARNLGRKAA
LEMLLTGEFIDAMEAKAKGLVNRVVPADALDAEIERLAGSILAKSAVAVRMGKGMFYQQLEMGLSEAYEYASEVMACNMM
SDDAGEGIDAFMQKRKPVYTGR
>Mature_261_residues
TQEEPLVLRQDRADGLTTLTLNRPGQFNSLSKDMLTAIQAELDAIAASDSVRVVVIAGAGKAFCAGHDLKEMRGNHSKAF
MQALFKQCGKLMLTITQMPQPVIARVHGIATAAGCQLVSMCDLAVAADVSKFAVSGINVGLFCSTPAVGLARNLGRKAAL
EMLLTGEFIDAMEAKAKGLVNRVVPADALDAEIERLAGSILAKSAVAVRMGKGMFYQQLEMGLSEAYEYASEVMACNMMS
DDAGEGIDAFMQKRKPVYTGR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=258, Percent_Identity=45.7364341085271, Blast_Score=233, Evalue=1e-61,
Organism=Homo sapiens, GI194097323, Length=251, Percent_Identity=32.2709163346614, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI4502327, Length=272, Percent_Identity=29.7794117647059, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI4757966, Length=231, Percent_Identity=26.4069264069264, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI51538581, Length=231, Percent_Identity=26.4069264069264, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI25453481, Length=231, Percent_Identity=26.4069264069264, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI51538573, Length=231, Percent_Identity=26.4069264069264, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI4757968, Length=231, Percent_Identity=25.5411255411255, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI49355787, Length=231, Percent_Identity=25.5411255411255, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI70995211, Length=254, Percent_Identity=27.1653543307087, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI221136756, Length=260, Percent_Identity=26.1538461538462, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI221136753, Length=260, Percent_Identity=26.1538461538462, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI221307494, Length=240, Percent_Identity=27.5, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI260274832, Length=249, Percent_Identity=24.4979919678715, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI45643119, Length=249, Percent_Identity=24.4979919678715, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI260275230, Length=249, Percent_Identity=24.4979919678715, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1787659, Length=262, Percent_Identity=33.969465648855, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1788597, Length=254, Percent_Identity=34.251968503937, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787660, Length=261, Percent_Identity=28.735632183908, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI221142681, Length=261, Percent_Identity=32.183908045977, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI87082183, Length=258, Percent_Identity=26.3565891472868, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788682, Length=186, Percent_Identity=30.1075268817204, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=229, Percent_Identity=44.5414847161572, Blast_Score=179, Evalue=9e-46,
Organism=Caenorhabditis elegans, GI17554946, Length=247, Percent_Identity=31.5789473684211, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI25145438, Length=256, Percent_Identity=30.078125, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17540714, Length=209, Percent_Identity=29.6650717703349, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17560910, Length=255, Percent_Identity=29.0196078431373, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17536985, Length=262, Percent_Identity=24.8091603053435, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17549921, Length=216, Percent_Identity=25.462962962963, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=256, Percent_Identity=25, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=252, Percent_Identity=24.6031746031746, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI25141363, Length=227, Percent_Identity=27.7533039647577, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=197, Percent_Identity=28.4263959390863, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922422, Length=254, Percent_Identity=40.9448818897638, Blast_Score=189, Evalue=1e-48,
Organism=Drosophila melanogaster, GI20129971, Length=245, Percent_Identity=33.0612244897959, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24653477, Length=245, Percent_Identity=33.0612244897959, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24653139, Length=267, Percent_Identity=33.7078651685393, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI19920382, Length=253, Percent_Identity=28.0632411067194, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24650670, Length=239, Percent_Identity=28.4518828451883, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI21357171, Length=186, Percent_Identity=32.258064516129, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27913; Mature: 27782

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
6.1 %Met     (Translated Protein)
8.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQEEPLVLRQDRADGLTTLTLNRPGQFNSLSKDMLTAIQAELDAIAASDSVRVVVIAGA
CCCCCCCEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
GKAFCAGHDLKEMRGNHSKAFMQALFKQCGKLMLTITQMPQPVIARVHGIATAAGCQLVS
CCHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
MCDLAVAADVSKFAVSGINVGLFCSTPAVGLARNLGRKAALEMLLTGEFIDAMEAKAKGL
HHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNRVVPADALDAEIERLAGSILAKSAVAVRMGKGMFYQQLEMGLSEAYEYASEVMACNMM
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
SDDAGEGIDAFMQKRKPVYTGR
CCCCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TQEEPLVLRQDRADGLTTLTLNRPGQFNSLSKDMLTAIQAELDAIAASDSVRVVVIAGA
CCCCCCEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
GKAFCAGHDLKEMRGNHSKAFMQALFKQCGKLMLTITQMPQPVIARVHGIATAAGCQLVS
CCHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
MCDLAVAADVSKFAVSGINVGLFCSTPAVGLARNLGRKAALEMLLTGEFIDAMEAKAKGL
HHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNRVVPADALDAEIERLAGSILAKSAVAVRMGKGMFYQQLEMGLSEAYEYASEVMACNMM
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
SDDAGEGIDAFMQKRKPVYTGR
CCCCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]