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The map label for this gene is aspA [H]

Identifier: 71906121

GI number: 71906121

Start: 542936

End: 544360

Strand: Reverse

Name: aspA [H]

Synonym: Daro_0481

Alternate gene names: 71906121

Gene position: 544360-542936 (Counterclockwise)

Preceding gene: 71906126

Following gene: 71906120

Centisome position: 12.09

GC content: 64.7

Gene sequence:

>1425_bases
ATGACCACCACTCCCTTCCGCCTCGAGCATGACCTGCTTGGTGACCGCGAGGTTCCGACCGCCGCCTACTACGGGGTACA
CACCCTGCGAGCCCTTGAGAATTTCCCGATCACCGGCATCTCGATCGCCGTCTATCCCGACCTGATCCGCGCCCTGGCCC
AGATCAAGAAGGCCGCCGCCCAGGCCAACCAGCAACTCGGCCTGCTCGATGCCCGGCGCGCCGGCGCCATCGCCGAAGCC
TGCGGCGAGATCATCGACGGCCAGTGGCACAACCAGTTCGTCGTCGATGTCATTCAGGGCGGCGCCGGCACCTCGACCAA
CATGAATGCCAACGAGGTGATCGCCAACCGTGCGCTGGAACTGCTCGGCAAGACGCGCGGCGACTACAAGACGCTGCACC
CGAACGAACACGTCAACATGAGCCAGTCGACCAACGACGTCTATCCGACGGCGCTGAAGCTGGCCACCTATGTCGGCATC
TTCCGCCTGGTCGAGGCCATGGCTTATCTGCGCAAAGCTTTCGAGCGCAAGGCCGACGAGTTCGCCGACGTGCTGAAGAT
GGGCCGTACCCAGTTGCAGGACGCCGTGCCGATGACCCTCGGCCAGGAGTTCTCGACCTACGCCGTGATGTTGGGCGAGG
ACGAAGAACGCCTGAAGGAAGCCGCACTGCTCATCCGCGAAATGAATCTCGGTGCCACCGCCATTGGCACCGGCATCAAC
GCCCACCCGGACTACGCCCCGCTGGTCTGCCGCAAGCTGGCCGAAATCAGCGGCATCCCGGTCGTCACCGCGCCCAACCT
GATCGAGGCGACGCAGGATTGCGGCAGCTTCGTGCAACTGTCCGGCGTCCTGAAGCGCGTCGCCGTCAAGCTATCCAAGG
TCTGCAACGACCTGCGCCTGCTCTCTTCCGGCCCGCGTGCCGGCTTCAACGAGATCAATCTGCCGCCGCGCCAGGCCGGT
TCGTCGATCATGCCGGGCAAGGTCAATCCGGTGATCCCGGAAGTGGTCAACCAGATCGCCTTCGAAGTGATCGGCAACGA
CACCACCGTCACTTTCGCCGCCGAAGCCGGCCAGCTGCAGCTCAATGCCTTCGAGCCGATCATCGCCCACAGCCTGTTCA
AGAGCGCCCTGCACCTGACCAACGGCTGCCGCGTGCTGGCCGACCATTGCGTCAATGGCATCACCGCCAACCGCGATCAG
CTGCGCGAATCGGTCGAGCGCTCGATCGGCATCGTCACCGCCCTCAACCCGTACATTGGTTACGCCAACGCCACCGAAGT
CGCTGCCGAAGCCTTCCGTAGCGGGCGCGGCGTGGCCGAGGTGGTCATCGAACGCGGTCTGATGAGCCCGGAACAGCTGG
CCGAAGTGCTGCGTCCCGAGGTGCTGACCCGCCCACAAGTCATCCCCCTGCCTGTCGCCGCCTGA

Upstream 100 bases:

>100_bases
CCACTTGCAACGCCGACAACCCCTGGCACGCCATGTGCAATAAAACAAGGCGCCCACGGCCTTGATGCCGGCGGCCTTGA
ACAAACAAAGGAGACAAACG

Downstream 100 bases:

>100_bases
TCCGGGCGTCTTTCCACTCAGCATCCCAAGGAAACCATCATGAAAATGAATCGCCTGACCACCTGGATCGGCATCGCCAT
GCTACTCGGCGTCATCGTTG

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MTTTPFRLEHDLLGDREVPTAAYYGVHTLRALENFPITGISIAVYPDLIRALAQIKKAAAQANQQLGLLDARRAGAIAEA
CGEIIDGQWHNQFVVDVIQGGAGTSTNMNANEVIANRALELLGKTRGDYKTLHPNEHVNMSQSTNDVYPTALKLATYVGI
FRLVEAMAYLRKAFERKADEFADVLKMGRTQLQDAVPMTLGQEFSTYAVMLGEDEERLKEAALLIREMNLGATAIGTGIN
AHPDYAPLVCRKLAEISGIPVVTAPNLIEATQDCGSFVQLSGVLKRVAVKLSKVCNDLRLLSSGPRAGFNEINLPPRQAG
SSIMPGKVNPVIPEVVNQIAFEVIGNDTTVTFAAEAGQLQLNAFEPIIAHSLFKSALHLTNGCRVLADHCVNGITANRDQ
LRESVERSIGIVTALNPYIGYANATEVAAEAFRSGRGVAEVVIERGLMSPEQLAEVLRPEVLTRPQVIPLPVAA

Sequences:

>Translated_474_residues
MTTTPFRLEHDLLGDREVPTAAYYGVHTLRALENFPITGISIAVYPDLIRALAQIKKAAAQANQQLGLLDARRAGAIAEA
CGEIIDGQWHNQFVVDVIQGGAGTSTNMNANEVIANRALELLGKTRGDYKTLHPNEHVNMSQSTNDVYPTALKLATYVGI
FRLVEAMAYLRKAFERKADEFADVLKMGRTQLQDAVPMTLGQEFSTYAVMLGEDEERLKEAALLIREMNLGATAIGTGIN
AHPDYAPLVCRKLAEISGIPVVTAPNLIEATQDCGSFVQLSGVLKRVAVKLSKVCNDLRLLSSGPRAGFNEINLPPRQAG
SSIMPGKVNPVIPEVVNQIAFEVIGNDTTVTFAAEAGQLQLNAFEPIIAHSLFKSALHLTNGCRVLADHCVNGITANRDQ
LRESVERSIGIVTALNPYIGYANATEVAAEAFRSGRGVAEVVIERGLMSPEQLAEVLRPEVLTRPQVIPLPVAA
>Mature_473_residues
TTTPFRLEHDLLGDREVPTAAYYGVHTLRALENFPITGISIAVYPDLIRALAQIKKAAAQANQQLGLLDARRAGAIAEAC
GEIIDGQWHNQFVVDVIQGGAGTSTNMNANEVIANRALELLGKTRGDYKTLHPNEHVNMSQSTNDVYPTALKLATYVGIF
RLVEAMAYLRKAFERKADEFADVLKMGRTQLQDAVPMTLGQEFSTYAVMLGEDEERLKEAALLIREMNLGATAIGTGINA
HPDYAPLVCRKLAEISGIPVVTAPNLIEATQDCGSFVQLSGVLKRVAVKLSKVCNDLRLLSSGPRAGFNEINLPPRQAGS
SIMPGKVNPVIPEVVNQIAFEVIGNDTTVTFAAEAGQLQLNAFEPIIAHSLFKSALHLTNGCRVLADHCVNGITANRDQL
RESVERSIGIVTALNPYIGYANATEVAAEAFRSGRGVAEVVIERGLMSPEQLAEVLRPEVLTRPQVIPLPVAA

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=462, Percent_Identity=41.5584415584416, Blast_Score=365, Evalue=1e-101,
Organism=Escherichia coli, GI87082375, Length=461, Percent_Identity=57.2668112798265, Blast_Score=523, Evalue=1e-150,
Organism=Escherichia coli, GI1787896, Length=457, Percent_Identity=41.3566739606127, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1790398, Length=293, Percent_Identity=27.9863481228669, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17553882, Length=469, Percent_Identity=42.0042643923241, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI32565146, Length=328, Percent_Identity=40.8536585365854, Blast_Score=275, Evalue=4e-74,
Organism=Saccharomyces cerevisiae, GI6324993, Length=464, Percent_Identity=39.4396551724138, Blast_Score=353, Evalue=5e-98,
Organism=Drosophila melanogaster, GI24640179, Length=468, Percent_Identity=41.2393162393162, Blast_Score=384, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24640177, Length=468, Percent_Identity=41.025641025641, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI78710009, Length=464, Percent_Identity=40.301724137931, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24662684, Length=462, Percent_Identity=41.1255411255411, Blast_Score=358, Evalue=3e-99,
Organism=Drosophila melanogaster, GI24583245, Length=463, Percent_Identity=36.2850971922246, Blast_Score=329, Evalue=2e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 51180; Mature: 51049

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTPFRLEHDLLGDREVPTAAYYGVHTLRALENFPITGISIAVYPDLIRALAQIKKAAA
CCCCCCEECHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHH
QANQQLGLLDARRAGAIAEACGEIIDGQWHNQFVVDVIQGGAGTSTNMNANEVIANRALE
HHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
LLGKTRGDYKTLHPNEHVNMSQSTNDVYPTALKLATYVGIFRLVEAMAYLRKAFERKADE
HHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FADVLKMGRTQLQDAVPMTLGQEFSTYAVMLGEDEERLKEAALLIREMNLGATAIGTGIN
HHHHHHHHHHHHHHHCCHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCHHHCCCCC
AHPDYAPLVCRKLAEISGIPVVTAPNLIEATQDCGSFVQLSGVLKRVAVKLSKVCNDLRL
CCCCHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSSGPRAGFNEINLPPRQAGSSIMPGKVNPVIPEVVNQIAFEVIGNDTTVTFAAEAGQLQ
HHCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCEEE
LNAFEPIIAHSLFKSALHLTNGCRVLADHCVNGITANRDQLRESVERSIGIVTALNPYIG
ECHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCHHEECCCCCC
YANATEVAAEAFRSGRGVAEVVIERGLMSPEQLAEVLRPEVLTRPQVIPLPVAA
CCCHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHCHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TTTPFRLEHDLLGDREVPTAAYYGVHTLRALENFPITGISIAVYPDLIRALAQIKKAAA
CCCCCEECHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHH
QANQQLGLLDARRAGAIAEACGEIIDGQWHNQFVVDVIQGGAGTSTNMNANEVIANRALE
HHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
LLGKTRGDYKTLHPNEHVNMSQSTNDVYPTALKLATYVGIFRLVEAMAYLRKAFERKADE
HHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FADVLKMGRTQLQDAVPMTLGQEFSTYAVMLGEDEERLKEAALLIREMNLGATAIGTGIN
HHHHHHHHHHHHHHHCCHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCHHHCCCCC
AHPDYAPLVCRKLAEISGIPVVTAPNLIEATQDCGSFVQLSGVLKRVAVKLSKVCNDLRL
CCCCHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSSGPRAGFNEINLPPRQAGSSIMPGKVNPVIPEVVNQIAFEVIGNDTTVTFAAEAGQLQ
HHCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCEEE
LNAFEPIIAHSLFKSALHLTNGCRVLADHCVNGITANRDQLRESVERSIGIVTALNPYIG
ECHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCHHEECCCCCC
YANATEVAAEAFRSGRGVAEVVIERGLMSPEQLAEVLRPEVLTRPQVIPLPVAA
CCCHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHCHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]