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The map label for this gene is dctA2 [H]

Identifier: 71906120

GI number: 71906120

Start: 541595

End: 542896

Strand: Reverse

Name: dctA2 [H]

Synonym: Daro_0480

Alternate gene names: 71906120

Gene position: 542896-541595 (Counterclockwise)

Preceding gene: 71906121

Following gene: 71906119

Centisome position: 12.06

GC content: 63.67

Gene sequence:

>1302_bases
ATGAAAATGAATCGCCTGACCACCTGGATCGGCATCGCCATGCTACTCGGCGTCATCGTTGGCTATGCCTGCAATGCGCT
CGCCCCGTCACCGGCCGCCGCCAAGGAAATCGCCGGCTACTTCGGCATCCTGACCGACATCTTCCTGCGCCTGATCAAGA
TGATCATCGCCCCGCTCGTCTTCGCCACCCTGGTCGCCGGGCTGGCCAACATGGGCGACTCGAAAACGGTCGGCCGCATC
GGCGGCAAGGCACTCGGCTGGTTCGTCGGCGCCTCCTTCTGCTCGCTGCTGCTTGGCCTGATCTTTGCCAACCTGCTGCA
ACCGGGCGTCGGCCTCAGCGTGCCGCTGCCGGAAGCGAGCGCGACACTGAATCTGAAAACCAGCGCGCTGAACCTGAAGG
ATTTCATCACCCATGTCTTCCCGAAGAACATCTTCGAAGCCATGGCGGCCAATGAAATCCTGCAGATCCTGGTCTTCGCC
GTGTTCTTCGGCCTGGCCCTCGGCCATCTGCACAACCAGATGGCGCGCAGCCTGGTGTGCACGATGGAGGAAGTCGTCCA
TGTCATGCTCAAGGTCACCGACTACGTGATGCGCTTCGCCCCGGTCGGGGTGTTTGGCGCAGTGGCCGGTGCGATCACGA
CCAACGGCCTGGGCATGCTGCTCGTTTTCGGCAAGCTGCTGGTCAGCTTCTACGCCGCCCTGGCCGCGCTGTGGATCGTG
CTGATCACTGCCGGTTACTTCGTGCTCGGCAAGGACATCTTCCGCCTGCTCAAGCTGATCCGCGGTCCGATGCTGGTCGG
TTTCTCGACGGCGAGCAGCGAATCGGTTTATCCGAAGCTGATGGAGCAGCTGGAAAAGTTCGGCATCCGCAACCGCGTCG
TCAGCTTCGTGCTGCCGCTCGGCTACTCGTTCAACCTCGACGGCTCGATGATCTACACCACCTTTGCCGCCCTGTTCGTG
GCCCAGGCCTACGATATCCCGATGACGCTGACCGCCCAGATCACCATGCTGCTGGTCCTGATGGTCTCGTCCAAGGGCAT
CGCCGGCGTGCCGCGCGCCTCGCTGGTCGTCGTCGCCGCCGTGCTGCCCATGTTCAACCTGCCGGAAGCCGGCCTGCTGC
TGGTGCTCGGCATCGACCATTTCCTCGACATGGGGCGCACCGTCACCAACGTGCTGGGCAACGCCATCGCCACCACGGTG
GTCGCCAAGTGGGAAAACGCCATCGACCCGGTCCCTCCCGAACTGGCCGACGCCGACGAGGCGCTGCCGGTCCCTGAAGA
GCCTGCCGCCCTGGCCGCCTGA

Upstream 100 bases:

>100_bases
AGTGCTGCGTCCCGAGGTGCTGACCCGCCCACAAGTCATCCCCCTGCCTGTCGCCGCCTGATCCGGGCGTCTTTCCACTC
AGCATCCCAAGGAAACCATC

Downstream 100 bases:

>100_bases
GTCTTTTTGTCTCCTCCGGGTTCGACTCAACCCTCGAACGTCGAACCCGCTTGTTTGCCCGGTGCACCGCACCGGGCTTT
TTTTCCCCTATCCTGACAAC

Product: Sodium:dicarboxylate symporter

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MKMNRLTTWIGIAMLLGVIVGYACNALAPSPAAAKEIAGYFGILTDIFLRLIKMIIAPLVFATLVAGLANMGDSKTVGRI
GGKALGWFVGASFCSLLLGLIFANLLQPGVGLSVPLPEASATLNLKTSALNLKDFITHVFPKNIFEAMAANEILQILVFA
VFFGLALGHLHNQMARSLVCTMEEVVHVMLKVTDYVMRFAPVGVFGAVAGAITTNGLGMLLVFGKLLVSFYAALAALWIV
LITAGYFVLGKDIFRLLKLIRGPMLVGFSTASSESVYPKLMEQLEKFGIRNRVVSFVLPLGYSFNLDGSMIYTTFAALFV
AQAYDIPMTLTAQITMLLVLMVSSKGIAGVPRASLVVVAAVLPMFNLPEAGLLLVLGIDHFLDMGRTVTNVLGNAIATTV
VAKWENAIDPVPPELADADEALPVPEEPAALAA

Sequences:

>Translated_433_residues
MKMNRLTTWIGIAMLLGVIVGYACNALAPSPAAAKEIAGYFGILTDIFLRLIKMIIAPLVFATLVAGLANMGDSKTVGRI
GGKALGWFVGASFCSLLLGLIFANLLQPGVGLSVPLPEASATLNLKTSALNLKDFITHVFPKNIFEAMAANEILQILVFA
VFFGLALGHLHNQMARSLVCTMEEVVHVMLKVTDYVMRFAPVGVFGAVAGAITTNGLGMLLVFGKLLVSFYAALAALWIV
LITAGYFVLGKDIFRLLKLIRGPMLVGFSTASSESVYPKLMEQLEKFGIRNRVVSFVLPLGYSFNLDGSMIYTTFAALFV
AQAYDIPMTLTAQITMLLVLMVSSKGIAGVPRASLVVVAAVLPMFNLPEAGLLLVLGIDHFLDMGRTVTNVLGNAIATTV
VAKWENAIDPVPPELADADEALPVPEEPAALAA
>Mature_433_residues
MKMNRLTTWIGIAMLLGVIVGYACNALAPSPAAAKEIAGYFGILTDIFLRLIKMIIAPLVFATLVAGLANMGDSKTVGRI
GGKALGWFVGASFCSLLLGLIFANLLQPGVGLSVPLPEASATLNLKTSALNLKDFITHVFPKNIFEAMAANEILQILVFA
VFFGLALGHLHNQMARSLVCTMEEVVHVMLKVTDYVMRFAPVGVFGAVAGAITTNGLGMLLVFGKLLVSFYAALAALWIV
LITAGYFVLGKDIFRLLKLIRGPMLVGFSTASSESVYPKLMEQLEKFGIRNRVVSFVLPLGYSFNLDGSMIYTTFAALFV
AQAYDIPMTLTAQITMLLVLMVSSKGIAGVPRASLVVVAAVLPMFNLPEAGLLLVLGIDHFLDMGRTVTNVLGNAIATTV
VAKWENAIDPVPPELADADEALPVPEEPAALAA

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=446, Percent_Identity=25.7847533632287, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI21314632, Length=430, Percent_Identity=26.9767441860465, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI66773030, Length=451, Percent_Identity=25.2771618625277, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI5032093, Length=414, Percent_Identity=27.0531400966184, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI40254478, Length=473, Percent_Identity=26.215644820296, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI4827012, Length=332, Percent_Identity=28.6144578313253, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI223468564, Length=289, Percent_Identity=29.4117647058824, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI223468566, Length=288, Percent_Identity=29.5138888888889, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI194239697, Length=257, Percent_Identity=29.1828793774319, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI262359914, Length=381, Percent_Identity=23.6220472440945, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI301601644, Length=177, Percent_Identity=30.5084745762712, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1789947, Length=383, Percent_Identity=42.0365535248042, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1790514, Length=371, Percent_Identity=40.1617250673854, Blast_Score=271, Evalue=8e-74,
Organism=Escherichia coli, GI1788024, Length=422, Percent_Identity=24.1706161137441, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789473, Length=232, Percent_Identity=28.448275862069, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17537407, Length=473, Percent_Identity=26.4270613107822, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI193206505, Length=457, Percent_Identity=26.0393873085339, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI71996953, Length=443, Percent_Identity=23.7020316027088, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI193206654, Length=486, Percent_Identity=24.8971193415638, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI71983099, Length=467, Percent_Identity=23.982869379015, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71983106, Length=449, Percent_Identity=24.2761692650334, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17541374, Length=408, Percent_Identity=25.7352941176471, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24583025, Length=477, Percent_Identity=23.2704402515723, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17137668, Length=477, Percent_Identity=23.2704402515723, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24583023, Length=477, Percent_Identity=23.2704402515723, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI281360483, Length=452, Percent_Identity=27.212389380531, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17137666, Length=452, Percent_Identity=27.212389380531, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI281360481, Length=451, Percent_Identity=27.2727272727273, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 46096; Mature: 46096

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCC
>Mature Secondary Structure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CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA