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The map label for this gene is glcD [H]

Identifier: 71905910

GI number: 71905910

Start: 312773

End: 314161

Strand: Reverse

Name: glcD [H]

Synonym: Daro_0268

Alternate gene names: 71905910

Gene position: 314161-312773 (Counterclockwise)

Preceding gene: 71905911

Following gene: 71905909

Centisome position: 6.98

GC content: 63.64

Gene sequence:

>1389_bases
GTGACCAGAAAAGCCCTCCCCGAAGCACTGCAGCAAGCCCTCCAGCAACAATTCCAGCAACGTTTTTCGCAAGGCGAATC
GACCCGCCTGCAGCATGGCCGCGACGAATCCGTTCATGCGCCTGAACTGCCCGACGGCGTCGTCCTGGCCGAAAGCACCG
ACGAAGTGGCACAGGTTGTCCGCCTGTGCGCCGAACATGGCGTCCCGGTCATCCCCTATGGCGTCGGCAGTTCGGTCGAG
GGCCACGTGCTGGCGACCAGCGGCGGCATTTCGCTCGATCTTTCCGGCATGAACCAGGTGATCGCCATCCACGCCGAAGA
TGGCGACGCCACGGTGCAGGCCGGCGTCACCCGCAAGCAGCTGAACGATGCCCTGAAGGGCAGCGGCCTGTTCTTTCCGA
TCGACCCCGGCGCCGACGCAACAATTGGCGGCATGGCCGCCACCCGGGCTTCCGGCACCAATGCTGTCCGTTACGGCACG
ATGCGTGAAAACGTGCTGGCTACCACCACGGTGCTGGCCGATGGCCGCGTGATGAAAACCGGCGGCCGCGCCCGCAAGTC
CTCCGCCGGTTACGACCTGACCCGCTTGCTGGTCGGATCCGAAGGCACGCTCGGCATCATGACCGAACTGACGGTCAAGC
TCTACCCGATCCCGGAGGCCATTTCGGCCGCCGTGTGCACCTTCCCGAGCATCGAAGCGGCGGTGCAGACGGTGATCCAG
ACCATCCAGCTCGGCGTGCCGGTCGCCCGCATCGAACTGCTCGACGCGCTTTCGCTGAAGGCCATCAACCTGTTCAGCAA
GACCACGCTGGCCGAGGCGCCGACGCTGTTCTTCGAATTTCATGGCAGCCCATCCTCCGCCACCGAGCAGGCCGAAACCG
TGCAGGCCATTGCCGACGAACTGGGCGGCACCAATTTCGAATGGGCCGCCCGGCCGGAAGACCGCAGCCGCCTGTGGCAG
GCCCGTCATGATGCCTATTTCGCCTGCCTGCAATTGAAGCCGGGCTGCCGCTGCTTCCCGACCGATGTCTGCGTGCCGAT
CTCGCGACTGGCCGAATGCATTGCCGAAACGCAGGCCGACATTGCGCAGGTCAGCATTCCCATCGCGCTGTTCGGCCATG
TCGGCGATGGCAATTTCCATCTTGTCGTGCTGGTTGATACCGACAACGCGAAAGAGATGAAAGAAGCCGCCTGGATCAGC
CAGCGGGTGGTCGAACGGGCCATTGCCATGGAAGGCACCTGCACCGGCGAGCATGGGATTGGGCTGGGCAAGCGCAAATA
CCTGCCGCTTGAACACGGCGACGTTGCCGTCGACGTGATGCGGACGATCAAACAGGCGCTCGATCCAAACAATCTGTTGA
ACCCGGGCAAAATCCTGCCCCCGGGTTAA

Upstream 100 bases:

>100_bases
TCCGTATCGGCGAACCAGATCTGGTTTGGCGAAAATTCGTCACCGCGCTCTATGAAGGGCATACTCACACGGATCTCGAC
CAAGCCACCCGATTCCTCCC

Downstream 100 bases:

>100_bases
CCCGCCTTGGCCGGAACGTTCTGTTTCGGCCAAACTCAAATCTCCAAAGTGCCCGTTGTCACCACCCCCGACGACAGGGC
GAACCACCACCGGCCCACAA

Product: FAD linked oxidase, C-terminal:FAD linked oxidase, N-terminal

Products: pyruvate; ferrocytochrome c

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MTRKALPEALQQALQQQFQQRFSQGESTRLQHGRDESVHAPELPDGVVLAESTDEVAQVVRLCAEHGVPVIPYGVGSSVE
GHVLATSGGISLDLSGMNQVIAIHAEDGDATVQAGVTRKQLNDALKGSGLFFPIDPGADATIGGMAATRASGTNAVRYGT
MRENVLATTTVLADGRVMKTGGRARKSSAGYDLTRLLVGSEGTLGIMTELTVKLYPIPEAISAAVCTFPSIEAAVQTVIQ
TIQLGVPVARIELLDALSLKAINLFSKTTLAEAPTLFFEFHGSPSSATEQAETVQAIADELGGTNFEWAARPEDRSRLWQ
ARHDAYFACLQLKPGCRCFPTDVCVPISRLAECIAETQADIAQVSIPIALFGHVGDGNFHLVVLVDTDNAKEMKEAAWIS
QRVVERAIAMEGTCTGEHGIGLGKRKYLPLEHGDVAVDVMRTIKQALDPNNLLNPGKILPPG

Sequences:

>Translated_462_residues
MTRKALPEALQQALQQQFQQRFSQGESTRLQHGRDESVHAPELPDGVVLAESTDEVAQVVRLCAEHGVPVIPYGVGSSVE
GHVLATSGGISLDLSGMNQVIAIHAEDGDATVQAGVTRKQLNDALKGSGLFFPIDPGADATIGGMAATRASGTNAVRYGT
MRENVLATTTVLADGRVMKTGGRARKSSAGYDLTRLLVGSEGTLGIMTELTVKLYPIPEAISAAVCTFPSIEAAVQTVIQ
TIQLGVPVARIELLDALSLKAINLFSKTTLAEAPTLFFEFHGSPSSATEQAETVQAIADELGGTNFEWAARPEDRSRLWQ
ARHDAYFACLQLKPGCRCFPTDVCVPISRLAECIAETQADIAQVSIPIALFGHVGDGNFHLVVLVDTDNAKEMKEAAWIS
QRVVERAIAMEGTCTGEHGIGLGKRKYLPLEHGDVAVDVMRTIKQALDPNNLLNPGKILPPG
>Mature_461_residues
TRKALPEALQQALQQQFQQRFSQGESTRLQHGRDESVHAPELPDGVVLAESTDEVAQVVRLCAEHGVPVIPYGVGSSVEG
HVLATSGGISLDLSGMNQVIAIHAEDGDATVQAGVTRKQLNDALKGSGLFFPIDPGADATIGGMAATRASGTNAVRYGTM
RENVLATTTVLADGRVMKTGGRARKSSAGYDLTRLLVGSEGTLGIMTELTVKLYPIPEAISAAVCTFPSIEAAVQTVIQT
IQLGVPVARIELLDALSLKAINLFSKTTLAEAPTLFFEFHGSPSSATEQAETVQAIADELGGTNFEWAARPEDRSRLWQA
RHDAYFACLQLKPGCRCFPTDVCVPISRLAECIAETQADIAQVSIPIALFGHVGDGNFHLVVLVDTDNAKEMKEAAWISQ
RVVERAIAMEGTCTGEHGIGLGKRKYLPLEHGDVAVDVMRTIKQALDPNNLLNPGKILPPG

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=434, Percent_Identity=51.3824884792627, Blast_Score=471, Evalue=1e-133,
Organism=Homo sapiens, GI37595754, Length=457, Percent_Identity=48.7964989059081, Blast_Score=461, Evalue=1e-130,
Organism=Homo sapiens, GI119964728, Length=419, Percent_Identity=29.8329355608592, Blast_Score=176, Evalue=6e-44,
Organism=Homo sapiens, GI4501993, Length=219, Percent_Identity=32.8767123287671, Blast_Score=111, Evalue=2e-24,
Organism=Escherichia coli, GI1789351, Length=419, Percent_Identity=30.3102625298329, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1787977, Length=533, Percent_Identity=27.9549718574109, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI48994907, Length=454, Percent_Identity=26.6519823788546, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI48994914, Length=135, Percent_Identity=34.0740740740741, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71992373, Length=473, Percent_Identity=46.9344608879493, Blast_Score=440, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI71992381, Length=473, Percent_Identity=45.877378435518, Blast_Score=421, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI71992392, Length=381, Percent_Identity=50.9186351706037, Blast_Score=398, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17534361, Length=432, Percent_Identity=26.1574074074074, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17556096, Length=498, Percent_Identity=24.8995983935743, Blast_Score=122, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6320027, Length=467, Percent_Identity=40.2569593147752, Blast_Score=327, Evalue=2e-90,
Organism=Saccharomyces cerevisiae, GI6320023, Length=436, Percent_Identity=26.605504587156, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6320764, Length=432, Percent_Identity=25.2314814814815, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI18921117, Length=419, Percent_Identity=28.8782816229117, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24639277, Length=419, Percent_Identity=28.8782816229117, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24639275, Length=419, Percent_Identity=28.8782816229117, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24653753, Length=460, Percent_Identity=23.9130434782609, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.2.4

Molecular weight: Translated: 49210; Mature: 49078

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKALPEALQQALQQQFQQRFSQGESTRLQHGRDESVHAPELPDGVVLAESTDEVAQVV
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCEEEECCHHHHHHHH
RLCAEHGVPVIPYGVGSSVEGHVLATSGGISLDLSGMNQVIAIHAEDGDATVQAGVTRKQ
HHHHHCCCCEEEECCCCCCCCEEEEECCCCEEEECCCCEEEEEEECCCCCEEECCCHHHH
LNDALKGSGLFFPIDPGADATIGGMAATRASGTNAVRYGTMRENVLATTTVLADGRVMKT
HHHHHCCCCEEEEECCCCCCCCCCHHHCCCCCCCEEEECCHHCCHHEEEEEEECCEEEEC
GGRARKSSAGYDLTRLLVGSEGTLGIMTELTVKLYPIPEAISAAVCTFPSIEAAVQTVIQ
CCCCCCCCCCCCEEEEEECCCCCEEEEEEEEEEEEECCHHHHHHHHCCCCHHHHHHHHHH
TIQLGVPVARIELLDALSLKAINLFSKTTLAEAPTLFFEFHGSPSSATEQAETVQAIADE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHH
LGGTNFEWAARPEDRSRLWQARHDAYFACLQLKPGCRCFPTDVCVPISRLAECIAETQAD
HCCCCCEECCCCCHHHHHHHHHCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
IAQVSIPIALFGHVGDGNFHLVVLVDTDNAKEMKEAAWISQRVVERAIAMEGTCTGEHGI
HHHEEEEEEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GLGKRKYLPLEHGDVAVDVMRTIKQALDPNNLLNPGKILPPG
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRKALPEALQQALQQQFQQRFSQGESTRLQHGRDESVHAPELPDGVVLAESTDEVAQVV
CHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCEEEECCHHHHHHHH
RLCAEHGVPVIPYGVGSSVEGHVLATSGGISLDLSGMNQVIAIHAEDGDATVQAGVTRKQ
HHHHHCCCCEEEECCCCCCCCEEEEECCCCEEEECCCCEEEEEEECCCCCEEECCCHHHH
LNDALKGSGLFFPIDPGADATIGGMAATRASGTNAVRYGTMRENVLATTTVLADGRVMKT
HHHHHCCCCEEEEECCCCCCCCCCHHHCCCCCCCEEEECCHHCCHHEEEEEEECCEEEEC
GGRARKSSAGYDLTRLLVGSEGTLGIMTELTVKLYPIPEAISAAVCTFPSIEAAVQTVIQ
CCCCCCCCCCCCEEEEEECCCCCEEEEEEEEEEEEECCHHHHHHHHCCCCHHHHHHHHHH
TIQLGVPVARIELLDALSLKAINLFSKTTLAEAPTLFFEFHGSPSSATEQAETVQAIADE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHH
LGGTNFEWAARPEDRSRLWQARHDAYFACLQLKPGCRCFPTDVCVPISRLAECIAETQAD
HCCCCCEECCCCCHHHHHHHHHCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
IAQVSIPIALFGHVGDGNFHLVVLVDTDNAKEMKEAAWISQRVVERAIAMEGTCTGEHGI
HHHEEEEEEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GLGKRKYLPLEHGDVAVDVMRTIKQALDPNNLLNPGKILPPG
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; ferricytochrome c

Specific reaction: (R)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]