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The map label for this gene is mak [H]

Identifier: 71905679

GI number: 71905679

Start: 43448

End: 44341

Strand: Reverse

Name: mak [H]

Synonym: Daro_0037

Alternate gene names: 71905679

Gene position: 44341-43448 (Counterclockwise)

Preceding gene: 71905680

Following gene: 71905678

Centisome position: 0.99

GC content: 62.53

Gene sequence:

>894_bases
ATGCGCCTCGGCATCGACCTTGGTGGGACCAAGATAGAGATCATCGCACTAGCCAGCGACGGTCACGAGTTGCTGCGCCA
GCGTATTGCAACGCCACAAGGTGATTACCTGGCCACCCTGATGGCCGTGGCTGGGCTGGTCGAGGCAAGCGAATCGAAAC
TCGGCCAGCGAGGCAGCGTCGGCGTCGGAATTCCCGGCGCCGAATCGCTGGCCACCGGGCTGATCAAGAATGCCAACTCG
ACCTGTCTGATCGGCAAGCCGCTCAAGCGCGACCTGCAGGCACTGTTGCGGCGAGAGGTCAGGCTGGCCAACGACGCCAA
CTGCTTTGCGCTGTCTGAAGCCATCGATGGCAGTGGTCGCGATGCCAATATCGTCTTCGGAATCATTCTTGGCACCGGCG
TCGGCGGCGGCATTGTGGTCAATCAACAGGTACTGGCCGGTGCCAATGCGATTGCCGGGGAATGGGGGCACAACCCATTG
CCCCTGGCAGCCGCTGACGATCTCCCCCTGCCCACCTGTTATTGCGGCCGGTCGGGGTGTATCGAAACCTATCTTTGCGG
CCCGGCGCTGACGACCGATCATTATCGCCATACCGGTGAAACGCTGGCGGCAGCCGAGATCGAGAAGCGCGCCATAGCCG
GCGATACGGCCTGCGAAGCCACGCTGCAGCGCTACGAGGACAGGCTGGGACGCGCCCTGGCTGGCGTCATCAACATCCTC
GATCCACAGGTCATTGTCATCGGTGGCGGCTTGTCGAACCTGCAACGGCTGTATCGCAACCTGCCTGAACGTTGCACCCC
GTACGTTTTCTCGGACGTCATCTACACGAAATTCCTGCCGCCCAGCCACGGGGATTCCTCGGGGGTCCGCGGGGCGGCGT
GGCTGTGGAATTGA

Upstream 100 bases:

>100_bases
AGCGCAACCTGTTCATGCTCCTGCTCGAAGCCGGCGCCAGCGTCCTGCGCACCCTGACCACCACCTACGGCAACGTCAAG
GGCATCGCCGTCCGGGTCTG

Downstream 100 bases:

>100_bases
TGGCAAAATCCGGAAACCATGGCAAACGTTTCCCTATTTATTGGCGGCATCCGGCCGCTCCCTGAATCCGGCCGACCAAC
CGGCATGTACAAGCAAGCGG

Product: N-acetylglucosamine kinase

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MRLGIDLGGTKIEIIALASDGHELLRQRIATPQGDYLATLMAVAGLVEASESKLGQRGSVGVGIPGAESLATGLIKNANS
TCLIGKPLKRDLQALLRREVRLANDANCFALSEAIDGSGRDANIVFGIILGTGVGGGIVVNQQVLAGANAIAGEWGHNPL
PLAAADDLPLPTCYCGRSGCIETYLCGPALTTDHYRHTGETLAAAEIEKRAIAGDTACEATLQRYEDRLGRALAGVINIL
DPQVIVIGGGLSNLQRLYRNLPERCTPYVFSDVIYTKFLPPSHGDSSGVRGAAWLWN

Sequences:

>Translated_297_residues
MRLGIDLGGTKIEIIALASDGHELLRQRIATPQGDYLATLMAVAGLVEASESKLGQRGSVGVGIPGAESLATGLIKNANS
TCLIGKPLKRDLQALLRREVRLANDANCFALSEAIDGSGRDANIVFGIILGTGVGGGIVVNQQVLAGANAIAGEWGHNPL
PLAAADDLPLPTCYCGRSGCIETYLCGPALTTDHYRHTGETLAAAEIEKRAIAGDTACEATLQRYEDRLGRALAGVINIL
DPQVIVIGGGLSNLQRLYRNLPERCTPYVFSDVIYTKFLPPSHGDSSGVRGAAWLWN
>Mature_297_residues
MRLGIDLGGTKIEIIALASDGHELLRQRIATPQGDYLATLMAVAGLVEASESKLGQRGSVGVGIPGAESLATGLIKNANS
TCLIGKPLKRDLQALLRREVRLANDANCFALSEAIDGSGRDANIVFGIILGTGVGGGIVVNQQVLAGANAIAGEWGHNPL
PLAAADDLPLPTCYCGRSGCIETYLCGPALTTDHYRHTGETLAAAEIEKRAIAGDTACEATLQRYEDRLGRALAGVINIL
DPQVIVIGGGLSNLQRLYRNLPERCTPYVFSDVIYTKFLPPSHGDSSGVRGAAWLWN

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI87081733, Length=300, Percent_Identity=51.6666666666667, Blast_Score=308, Evalue=2e-85,
Organism=Escherichia coli, GI1787363, Length=301, Percent_Identity=42.1926910299003, Blast_Score=212, Evalue=2e-56,
Organism=Escherichia coli, GI1786891, Length=245, Percent_Identity=24.8979591836735, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790522, Length=299, Percent_Identity=24.4147157190635, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787878, Length=241, Percent_Identity=24.0663900414938, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 31255; Mature: 31255

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLGIDLGGTKIEIIALASDGHELLRQRIATPQGDYLATLMAVAGLVEASESKLGQRGSV
CEEECCCCCCEEEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
GVGIPGAESLATGLIKNANSTCLIGKPLKRDLQALLRREVRLANDANCFALSEAIDGSGR
CCCCCCHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHCCCCC
DANIVFGIILGTGVGGGIVVNQQVLAGANAIAGEWGHNPLPLAAADDLPLPTCYCGRSGC
CCEEEEEEEEECCCCCCEEECHHHHHCCCHHCCCCCCCCCCCCCCCCCCCCEEECCCCCC
IETYLCGPALTTDHYRHTGETLAAAEIEKRAIAGDTACEATLQRYEDRLGRALAGVINIL
CHHEECCCCCCCCHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
DPQVIVIGGGLSNLQRLYRNLPERCTPYVFSDVIYTKFLPPSHGDSSGVRGAAWLWN
CCCEEEEECCHHHHHHHHHHCHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEECCC
>Mature Secondary Structure
MRLGIDLGGTKIEIIALASDGHELLRQRIATPQGDYLATLMAVAGLVEASESKLGQRGSV
CEEECCCCCCEEEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
GVGIPGAESLATGLIKNANSTCLIGKPLKRDLQALLRREVRLANDANCFALSEAIDGSGR
CCCCCCHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHCCCCC
DANIVFGIILGTGVGGGIVVNQQVLAGANAIAGEWGHNPLPLAAADDLPLPTCYCGRSGC
CCEEEEEEEEECCCCCCEEECHHHHHCCCHHCCCCCCCCCCCCCCCCCCCCEEECCCCCC
IETYLCGPALTTDHYRHTGETLAAAEIEKRAIAGDTACEATLQRYEDRLGRALAGVINIL
CHHEECCCCCCCCHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
DPQVIVIGGGLSNLQRLYRNLPERCTPYVFSDVIYTKFLPPSHGDSSGVRGAAWLWN
CCCEEEEECCHHHHHHHHHHCHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]