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The map label for this gene is aspS [H]

Identifier: 71911626

GI number: 71911626

Start: 1781584

End: 1783332

Strand: Reverse

Name: aspS [H]

Synonym: M5005_Spy_1813

Alternate gene names: 71911626

Gene position: 1783332-1781584 (Counterclockwise)

Preceding gene: 71911627

Following gene: 71911625

Centisome position: 97.0

GC content: 42.08

Gene sequence:

>1749_bases
ATGAAACGTAGCATGTATGCAGGGCGTGTTCGCGAAGAACACATTGGAACGACAATCACATTGAAAGGATGGGTCAGTCG
TCGTCGTGACTTGGGTGGTCTCATCTTTATTGATTTGCGTGACCGTGAGGGTGTGATGCAATTAGTCATTAACCCTGAGG
AAGTTTCCAGTAATGTGATGGCAACTGCTGAACGCTTACGTAGTGAATATGTGATTGAAGTGGAAGGTTTTGTTGAAGCT
CGACAACAAGCCAATGATAAGTTGGCGACAGGAATGGTTGAGTTAAAAGTATCTGCTTTGACCATTTTAAACACAGCTAA
AACGACGCCTTTTGAAATTAAAGATGACGTTGAAGTAAGTGATGACACGCGTTTACGTTACCGTTACTTAGATTTACGAC
GTCCAGAAATGTTAGAAAACTTCAAGCTACGTGCAAAAGTGACTCATTCTATCCGTAATTACCTTGATGACCTTGAGTTT
ATCGATGTCGAGACACCTATGTTGACAAAATCAACTCCAGAAGGTGCGCGTGACTATCTGGTACCAAGTCGTGTTAGCCA
AGGGCATTTCTATGCTTTGCCACAAAGTCCGCAAATAACCAAACAGTTATTGATGAATGCTGGCTTTGATCGCTATTATC
AAATTGTGAAATGTTTCCGCGATGAAGATCTACGTGGTGACCGTCAACCTGAGTTTACACAAGTTGACTTGGAAACGTCA
TTCCTTTCTGAACAAGAAATTCAAGACATCGTTGAAGGCATGATTGCTAAAGTGATGAAAGAGACAAAAGAAATAGATGT
AACCCTACCATTCCCACGTATGAGCTACGATGTAGCAATGAATAGCTACGGCTCTGATAAACCAGACACACGTTTTGAAA
TGCTTTTACAAGACTTGACAGTAACTGTCAAAGGAAATGACTTCAAGGTTTTCTCAGAAGCACCAGCTGTCAAGGCGATT
GTGGTGAAGGGAAATGCAGATCGATACTCTCGTAAAGATATTGACAAATTAACTGAGTTTGCCAAACAATTTGGTGCTAA
AGGCCTTGCCTGGGTTAAGGTGACAGATGGTCAATTGGCAGGCCCAGTAGCCAAGTTCTTGACAGCTATTGAGACAGAGT
TGTCAAGCCAATTAAAACTTGCTGAGAATGATTTGGTTTTATTTGTAGCGGATACTCTTGAGGTTGCTAATAATACATTA
GGTGCCCTTCGTAATCGTATTGCCAAGGACCTTGACATGATTGATCAGTCGCAGTTTAATTTCCTTTGGGTGGTTGATTG
GCCAATGTTTGAGTGGTCAGAAGAAGAAGGACGTTATATGTCAGCTCATCACCCCTTCACTCTTCCAACGCCAGAATCTG
CTCATGAGTTAGAGGGTGATTTGGCTAAGGTACGGGCTATTGCTTACGACATCGTTTTAAATGGTTACGAGCTTGGTGGT
GGTAGCCTGCGTATCAATCAAAAAGAGATGCAAGAGCGCATGTTCAAGGCTCTTGGCTTTACCGCTGATGAAGCTAACGA
CCAATTTGGTTTCTTACTAGAAGCGATGGACTACGGCTTTCCTCCTCATGGTGGCTTGGCAATTGGTCTTGACCGTTTTG
TCATGTTGCTTGCTGGAAAAGACAATATTCGAGAAGTTATTGCCTTCCCTAAAAATAACAAAGCCTCTGATCCAATGACG
CAAGCACCTAGTCTTGTTTCTGAAAACCAATTAGAAGAACTCAGTCTTCAAATAGAAAGTCATGATTAA

Upstream 100 bases:

>100_bases
TTGCTATAATAGTAGGGAATGAATTAATTGATTTATATAGTGAAAGGAATTGGAAACGAGCAGGTTTCACAGGATTCTGT
TTGTTTTAGGGAATTTATTT

Downstream 100 bases:

>100_bases
GAAAACAACCTATAAGAAAAAAGTTAAATATGTCATTAGTAGAGGGGCCAAGAAAGTTGGCCTACTCCACGCTCTAAGAA
GTATTTCAAGAGAAAAATAT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 582; Mature: 582

Protein sequence:

>582_residues
MKRSMYAGRVREEHIGTTITLKGWVSRRRDLGGLIFIDLRDREGVMQLVINPEEVSSNVMATAERLRSEYVIEVEGFVEA
RQQANDKLATGMVELKVSALTILNTAKTTPFEIKDDVEVSDDTRLRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDDLEF
IDVETPMLTKSTPEGARDYLVPSRVSQGHFYALPQSPQITKQLLMNAGFDRYYQIVKCFRDEDLRGDRQPEFTQVDLETS
FLSEQEIQDIVEGMIAKVMKETKEIDVTLPFPRMSYDVAMNSYGSDKPDTRFEMLLQDLTVTVKGNDFKVFSEAPAVKAI
VVKGNADRYSRKDIDKLTEFAKQFGAKGLAWVKVTDGQLAGPVAKFLTAIETELSSQLKLAENDLVLFVADTLEVANNTL
GALRNRIAKDLDMIDQSQFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPTPESAHELEGDLAKVRAIAYDIVLNGYELGG
GSLRINQKEMQERMFKALGFTADEANDQFGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKDNIREVIAFPKNNKASDPMT
QAPSLVSENQLEELSLQIESHD

Sequences:

>Translated_582_residues
MKRSMYAGRVREEHIGTTITLKGWVSRRRDLGGLIFIDLRDREGVMQLVINPEEVSSNVMATAERLRSEYVIEVEGFVEA
RQQANDKLATGMVELKVSALTILNTAKTTPFEIKDDVEVSDDTRLRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDDLEF
IDVETPMLTKSTPEGARDYLVPSRVSQGHFYALPQSPQITKQLLMNAGFDRYYQIVKCFRDEDLRGDRQPEFTQVDLETS
FLSEQEIQDIVEGMIAKVMKETKEIDVTLPFPRMSYDVAMNSYGSDKPDTRFEMLLQDLTVTVKGNDFKVFSEAPAVKAI
VVKGNADRYSRKDIDKLTEFAKQFGAKGLAWVKVTDGQLAGPVAKFLTAIETELSSQLKLAENDLVLFVADTLEVANNTL
GALRNRIAKDLDMIDQSQFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPTPESAHELEGDLAKVRAIAYDIVLNGYELGG
GSLRINQKEMQERMFKALGFTADEANDQFGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKDNIREVIAFPKNNKASDPMT
QAPSLVSENQLEELSLQIESHD
>Mature_582_residues
MKRSMYAGRVREEHIGTTITLKGWVSRRRDLGGLIFIDLRDREGVMQLVINPEEVSSNVMATAERLRSEYVIEVEGFVEA
RQQANDKLATGMVELKVSALTILNTAKTTPFEIKDDVEVSDDTRLRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDDLEF
IDVETPMLTKSTPEGARDYLVPSRVSQGHFYALPQSPQITKQLLMNAGFDRYYQIVKCFRDEDLRGDRQPEFTQVDLETS
FLSEQEIQDIVEGMIAKVMKETKEIDVTLPFPRMSYDVAMNSYGSDKPDTRFEMLLQDLTVTVKGNDFKVFSEAPAVKAI
VVKGNADRYSRKDIDKLTEFAKQFGAKGLAWVKVTDGQLAGPVAKFLTAIETELSSQLKLAENDLVLFVADTLEVANNTL
GALRNRIAKDLDMIDQSQFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPTPESAHELEGDLAKVRAIAYDIVLNGYELGG
GSLRINQKEMQERMFKALGFTADEANDQFGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKDNIREVIAFPKNNKASDPMT
QAPSLVSENQLEELSLQIESHD

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=592, Percent_Identity=40.3716216216216, Blast_Score=425, Evalue=1e-119,
Organism=Homo sapiens, GI5031815, Length=285, Percent_Identity=27.0175438596491, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI194272210, Length=285, Percent_Identity=27.0175438596491, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI45439306, Length=275, Percent_Identity=26.9090909090909, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI1788173, Length=590, Percent_Identity=49.1525423728814, Blast_Score=563, Evalue=1e-162,
Organism=Escherichia coli, GI1789256, Length=294, Percent_Identity=25.5102040816327, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI1790571, Length=291, Percent_Identity=23.3676975945017, Blast_Score=70, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI32566633, Length=589, Percent_Identity=35.9932088285229, Blast_Score=322, Evalue=5e-88,
Organism=Caenorhabditis elegans, GI71994340, Length=310, Percent_Identity=22.5806451612903, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17535925, Length=308, Percent_Identity=22.7272727272727, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17535927, Length=310, Percent_Identity=22.5806451612903, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17551876, Length=264, Percent_Identity=25.3787878787879, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71984122, Length=302, Percent_Identity=24.8344370860927, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325153, Length=626, Percent_Identity=32.1086261980831, Blast_Score=285, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6323011, Length=241, Percent_Identity=29.045643153527, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=197, Percent_Identity=29.4416243654822, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6321807, Length=267, Percent_Identity=24.7191011235955, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24584738, Length=600, Percent_Identity=36, Blast_Score=348, Evalue=6e-96,
Organism=Drosophila melanogaster, GI17136276, Length=239, Percent_Identity=28.4518828451883, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24640849, Length=286, Percent_Identity=26.9230769230769, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24640851, Length=286, Percent_Identity=26.9230769230769, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 66028; Mature: 66028

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRSMYAGRVREEHIGTTITLKGWVSRRRDLGGLIFIDLRDREGVMQLVINPEEVSSNVM
CCCCCCCCCCHHHHCCCEEEEHHHHHHHCCCCCEEEEEECCCCCEEEEEECHHHHHHHHH
ATAERLRSEYVIEVEGFVEARQQANDKLATGMVELKVSALTILNTAKTTPFEIKDDVEVS
HHHHHHCCCEEEEEHHHHHHHHHCCHHHHHCCEEEEEEEEEEEECCCCCCCCCCCCCCCC
DDTRLRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDDLEFIDVETPMLTKSTPEGARDYL
CCCEEEEEEECCCCHHHHHCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHCC
VPSRVSQGHFYALPQSPQITKQLLMNAGFDRYYQIVKCFRDEDLRGDRQPEFTQVDLETS
CCCCCCCCEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEECHHH
FLSEQEIQDIVEGMIAKVMKETKEIDVTLPFPRMSYDVAMNSYGSDKPDTRFEMLLQDLT
HCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHEECCCCCCCCCHHHHHHHHHCE
VTVKGNDFKVFSEAPAVKAIVVKGNADRYSRKDIDKLTEFAKQFGAKGLAWVKVTDGQLA
EEEECCCEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHH
GPVAKFLTAIETELSSQLKLAENDLVLFVADTLEVANNTLGALRNRIAKDLDMIDQSQFN
HHHHHHHHHHHHHHHHHHEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
FLWVVDWPMFEWSEEEGRYMSAHHPFTLPTPESAHELEGDLAKVRAIAYDIVLNGYELGG
EEEEEECCCCCCCCCCCCEEECCCCEECCCCCCHHHHHHHHHHHHHHHEEEEEECEEECC
GSLRINQKEMQERMFKALGFTADEANDQFGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGK
CEEEECHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCEEECHHHHEEEECCC
DNIREVIAFPKNNKASDPMTQAPSLVSENQLEELSLQIESHD
CCHHHHHCCCCCCCCCCCCHHCCCHHHHCCCEEEEEEEECCC
>Mature Secondary Structure
MKRSMYAGRVREEHIGTTITLKGWVSRRRDLGGLIFIDLRDREGVMQLVINPEEVSSNVM
CCCCCCCCCCHHHHCCCEEEEHHHHHHHCCCCCEEEEEECCCCCEEEEEECHHHHHHHHH
ATAERLRSEYVIEVEGFVEARQQANDKLATGMVELKVSALTILNTAKTTPFEIKDDVEVS
HHHHHHCCCEEEEEHHHHHHHHHCCHHHHHCCEEEEEEEEEEEECCCCCCCCCCCCCCCC
DDTRLRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDDLEFIDVETPMLTKSTPEGARDYL
CCCEEEEEEECCCCHHHHHCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHCC
VPSRVSQGHFYALPQSPQITKQLLMNAGFDRYYQIVKCFRDEDLRGDRQPEFTQVDLETS
CCCCCCCCEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEECHHH
FLSEQEIQDIVEGMIAKVMKETKEIDVTLPFPRMSYDVAMNSYGSDKPDTRFEMLLQDLT
HCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHEECCCCCCCCCHHHHHHHHHCE
VTVKGNDFKVFSEAPAVKAIVVKGNADRYSRKDIDKLTEFAKQFGAKGLAWVKVTDGQLA
EEEECCCEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHH
GPVAKFLTAIETELSSQLKLAENDLVLFVADTLEVANNTLGALRNRIAKDLDMIDQSQFN
HHHHHHHHHHHHHHHHHHEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
FLWVVDWPMFEWSEEEGRYMSAHHPFTLPTPESAHELEGDLAKVRAIAYDIVLNGYELGG
EEEEEECCCCCCCCCCCCEEECCCCEECCCCCCHHHHHHHHHHHHHHHEEEEEECEEECC
GSLRINQKEMQERMFKALGFTADEANDQFGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGK
CEEEECHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCEEECHHHHEEEECCC
DNIREVIAFPKNNKASDPMTQAPSLVSENQLEELSLQIESHD
CCHHHHHCCCCCCCCCCCCHHCCCHHHHCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]