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The map label for this gene is pflD [H]

Identifier: 71911556

GI number: 71911556

Start: 1703764

End: 1706136

Strand: Reverse

Name: pflD [H]

Synonym: M5005_Spy_1743

Alternate gene names: 71911556

Gene position: 1706136-1703764 (Counterclockwise)

Preceding gene: 71911557

Following gene: 71911555

Centisome position: 92.8

GC content: 44.63

Gene sequence:

>2373_bases
ATGACTCACTATCGTGAAGCTGTGTTAGACAAGAAACCTTATATTGATGCTGAGCGTGCTATTTTAGCCACTGAAGCCTA
TCAAAAACACCAAAATAAGCCAGCAAACCTCAAACGCGCTTACATGCTTCAAACTATTTTGGAAAACATGACCATCTATA
TTGAGGATGAAAGCCTCATTGCTGGTAACCAAGCCTCTTCAAACAAGGATGCACCGATTTTCCCAGAATACACTCTGGAA
TTTGTCCTCAACGAACTTGATCTTTTTGAAAAACGAGATGGGGATGTCTTCTACATTACCGAAGAAACCAAGCAACAGCT
CCGAGACATTGCCCCATTCTGGGAAAACAATAACCTGCGTGCTCGCTGCGGTGTCTTGCTACCAGAAGAAGTGCAAGTGT
ACATGGAAACAGGTTTCTTTGGCATGGAAGGCAAGATGAACTCTGGGGATGCTCATTTAGCGGTTAATTACCAAAAACTC
TTGGAGCATGGCTTAAAGGGATTTGAAGAACGGGCACGAGCTGCCAAAGCTGCGCTTGATTTGACCATTCCTGAAAACAT
TGACAAATACCATTTTTACGACTCTGTTTTTATTGTCATTGATGCAGTCAAAACTTATGCTAAACGCTATGCCAAGTTGG
CTAGAGAACTAGCAAAAACGGCTAAACCAGAACGTCAAGCAGAATTGTTAGACATCGCGCGCATTTGTGATAAAGTACCT
TACGAACCAGCGAAAACCTTTGCAGAGGCTGTGCAATCTGTCTGGTTCATTCAGTGTATTTTGCAAATTGAATCAAATGG
GCATTCCCTATCTTATGGCCGCTTTGACCAGTACATGTATCCTTATGTCAAAGCTGATTTGGAGGCTGGCCGTGAAACAG
AAGACACAATTGTTGAACGTTTGACCAACCTCTGGATTAAGACCTTGACCATTAATAAAGTACGGAGCCAAGCCCACACC
TTCTCATCAGCAGGTAGTCCACTTTACCAAAACGTGACAATCGGCGGTCAAACACGCGATAAAAAAGATGCGGTCAACCC
ACTGTCTTACCTTGTCCTACGGAGTGTGGCTCAAACCAAATTGCCACAACCTAACTTGACTGTTCGTTACCACAAAGGCC
TAGACAATACCTTTATGAACGAATGTATCGAGGTCATGAAACTTGGTTTTGGTATGCCAGCCATGAACAACGATGAAATC
ATCATTCCATCTTTCATCAAAAAAGGTGTCTCAGAAGAAGATGCTTATGATTACTCAGCCATTGGCTGTGTGGAAACAGC
AGTTCCTGGAAAATGGGGCTACCGTTGCACGGGGATGAGTTACATCAACTTCCCTAAAATCTTGCTGATTACCATGAATG
ACGGGATTGACCCAGCATCAGGCAAGCGCTTTGCAAAAGGACACGGTCACTTCAAAGACATGACCTCTTATGAAGAATTA
AAAGCAGCATGGGATGCCACCCTGCGCGAAATTACCCGCATGAGCGTCATTGTTGAAAATGCCATTGACCTCGGTCTTGA
ACGTGAAGTACCAGATATTCTTTGCTCAGCCTTGACAGATGATTGTATCGGTCGTGGCAAGACCTTAAAAGAAGGCGGAG
CTGTTTACGACTATATCTCAGGCCTTCAAGTTGGTATTGCCAACCTATCAGACTCCCTAGCAGCGCTGAAAAAATTAGTG
TTTGAAGAAGGTCGCTTGACCCCAGAGGAACTCTGGAAGGCCCTTGAAAGTGACTTTGCTGGAGAACGCGGAGAAGACAT
CCGCCAAATGCTCATCAATGACGCACCAAAATATGGTAATGATGATGACTACGCAGATAGTTTAGTCGTCGAAGCTTACG
ACACCTACATTGATGAAATTGCCAAATACCCAAATACCCGGTATGGCCGTGGCCCAATCGGAGGTATCCGCTATTCTGGG
ACATCATCAATCTCAGCCAATGTCGGACAAGGAAAAGGGACCTTGGCGACACCAGACGGTCGCCATGCAGGCACACCACT
AGCAGAAGGCTGTTCACCAGAACACAGCATGGACAAAAAAGGCCCGACTTCAGTATTGAAATCTGTTGCTAAATTACCAA
CAGATGAGATTGTCGGAGGAGTGCTACTCAACCAAAAAGTTAACCCACAAACCCTGGCCAAAGAAGAAGACAAACTGAAA
TTAATGGCCTTACTTCGTACCTTCTTTAATCGCCTACACGGTTATCATATCCAGTACAATGTCGTGTCACGTGAAACCTT
GATTGATGCCCAAAAACATCCTGAAAAGCACCGCGACCTCATTGTCCGTGTTGCCGGCTACTCAGCTTTCTTCAATGTGC
TCTCAAAAGCAACCCAAGATGACATTATTGAACGTACAGAACACACACTTTAG

Upstream 100 bases:

>100_bases
TAAAATAGTTACGAAAGAAACGTTTATAATTTCGTAAGAAAGAAGGAGACCCCGCATGACTGAAACAAAAAGCCCTTATT
TTGGACATTTAACAGACAGA

Downstream 100 bases:

>100_bases
GAGAAAATAAGTATGGAATACATGTTAGACACTTTAGATTTAGAAGCTATTAAAAAATGGCATCACATTTTGCCTCTCGC
AGGTGTGACCTCAAACCCTT

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase 3 [H]

Number of amino acids: Translated: 790; Mature: 789

Protein sequence:

>790_residues
MTHYREAVLDKKPYIDAERAILATEAYQKHQNKPANLKRAYMLQTILENMTIYIEDESLIAGNQASSNKDAPIFPEYTLE
FVLNELDLFEKRDGDVFYITEETKQQLRDIAPFWENNNLRARCGVLLPEEVQVYMETGFFGMEGKMNSGDAHLAVNYQKL
LEHGLKGFEERARAAKAALDLTIPENIDKYHFYDSVFIVIDAVKTYAKRYAKLARELAKTAKPERQAELLDIARICDKVP
YEPAKTFAEAVQSVWFIQCILQIESNGHSLSYGRFDQYMYPYVKADLEAGRETEDTIVERLTNLWIKTLTINKVRSQAHT
FSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLRSVAQTKLPQPNLTVRYHKGLDNTFMNECIEVMKLGFGMPAMNNDEI
IIPSFIKKGVSEEDAYDYSAIGCVETAVPGKWGYRCTGMSYINFPKILLITMNDGIDPASGKRFAKGHGHFKDMTSYEEL
KAAWDATLREITRMSVIVENAIDLGLEREVPDILCSALTDDCIGRGKTLKEGGAVYDYISGLQVGIANLSDSLAALKKLV
FEEGRLTPEELWKALESDFAGERGEDIRQMLINDAPKYGNDDDYADSLVVEAYDTYIDEIAKYPNTRYGRGPIGGIRYSG
TSSISANVGQGKGTLATPDGRHAGTPLAEGCSPEHSMDKKGPTSVLKSVAKLPTDEIVGGVLLNQKVNPQTLAKEEDKLK
LMALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSKATQDDIIERTEHTL

Sequences:

>Translated_790_residues
MTHYREAVLDKKPYIDAERAILATEAYQKHQNKPANLKRAYMLQTILENMTIYIEDESLIAGNQASSNKDAPIFPEYTLE
FVLNELDLFEKRDGDVFYITEETKQQLRDIAPFWENNNLRARCGVLLPEEVQVYMETGFFGMEGKMNSGDAHLAVNYQKL
LEHGLKGFEERARAAKAALDLTIPENIDKYHFYDSVFIVIDAVKTYAKRYAKLARELAKTAKPERQAELLDIARICDKVP
YEPAKTFAEAVQSVWFIQCILQIESNGHSLSYGRFDQYMYPYVKADLEAGRETEDTIVERLTNLWIKTLTINKVRSQAHT
FSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLRSVAQTKLPQPNLTVRYHKGLDNTFMNECIEVMKLGFGMPAMNNDEI
IIPSFIKKGVSEEDAYDYSAIGCVETAVPGKWGYRCTGMSYINFPKILLITMNDGIDPASGKRFAKGHGHFKDMTSYEEL
KAAWDATLREITRMSVIVENAIDLGLEREVPDILCSALTDDCIGRGKTLKEGGAVYDYISGLQVGIANLSDSLAALKKLV
FEEGRLTPEELWKALESDFAGERGEDIRQMLINDAPKYGNDDDYADSLVVEAYDTYIDEIAKYPNTRYGRGPIGGIRYSG
TSSISANVGQGKGTLATPDGRHAGTPLAEGCSPEHSMDKKGPTSVLKSVAKLPTDEIVGGVLLNQKVNPQTLAKEEDKLK
LMALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSKATQDDIIERTEHTL
>Mature_789_residues
THYREAVLDKKPYIDAERAILATEAYQKHQNKPANLKRAYMLQTILENMTIYIEDESLIAGNQASSNKDAPIFPEYTLEF
VLNELDLFEKRDGDVFYITEETKQQLRDIAPFWENNNLRARCGVLLPEEVQVYMETGFFGMEGKMNSGDAHLAVNYQKLL
EHGLKGFEERARAAKAALDLTIPENIDKYHFYDSVFIVIDAVKTYAKRYAKLARELAKTAKPERQAELLDIARICDKVPY
EPAKTFAEAVQSVWFIQCILQIESNGHSLSYGRFDQYMYPYVKADLEAGRETEDTIVERLTNLWIKTLTINKVRSQAHTF
SSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLRSVAQTKLPQPNLTVRYHKGLDNTFMNECIEVMKLGFGMPAMNNDEII
IPSFIKKGVSEEDAYDYSAIGCVETAVPGKWGYRCTGMSYINFPKILLITMNDGIDPASGKRFAKGHGHFKDMTSYEELK
AAWDATLREITRMSVIVENAIDLGLEREVPDILCSALTDDCIGRGKTLKEGGAVYDYISGLQVGIANLSDSLAALKKLVF
EEGRLTPEELWKALESDFAGERGEDIRQMLINDAPKYGNDDDYADSLVVEAYDTYIDEIAKYPNTRYGRGPIGGIRYSGT
SSISANVGQGKGTLATPDGRHAGTPLAEGCSPEHSMDKKGPTSVLKSVAKLPTDEIVGGVLLNQKVNPQTLAKEEDKLKL
MALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSKATQDDIIERTEHTL

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787044, Length=789, Percent_Identity=53.6121673003802, Blast_Score=852, Evalue=0.0,
Organism=Escherichia coli, GI1790388, Length=794, Percent_Identity=38.0352644836272, Blast_Score=490, Evalue=1e-139,
Organism=Escherichia coli, GI48994926, Length=590, Percent_Identity=27.9661016949153, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1787131, Length=559, Percent_Identity=27.7280858676207, Blast_Score=186, Evalue=7e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 88701; Mature: 88570

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00063 ALDOKETO_REDUCTASE_3 ; PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHYREAVLDKKPYIDAERAILATEAYQKHQNKPANLKRAYMLQTILENMTIYIEDESLI
CCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECCCEE
AGNQASSNKDAPIFPEYTLEFVLNELDLFEKRDGDVFYITEETKQQLRDIAPFWENNNLR
ECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCCCCCE
ARCGVLLPEEVQVYMETGFFGMEGKMNSGDAHLAVNYQKLLEHGLKGFEERARAAKAALD
EEECCCCHHHHHHHHHCCEECCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHEE
LTIPENIDKYHFYDSVFIVIDAVKTYAKRYAKLARELAKTAKPERQAELLDIARICDKVP
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
YEPAKTFAEAVQSVWFIQCILQIESNGHSLSYGRFDQYMYPYVKADLEAGRETEDTIVER
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
LTNLWIKTLTINKVRSQAHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLRSVAQTK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHC
LPQPNLTVRYHKGLDNTFMNECIEVMKLGFGMPAMNNDEIIIPSFIKKGVSEEDAYDYSA
CCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHCCCCCCCCCCHHH
IGCVETAVPGKWGYRCTGMSYINFPKILLITMNDGIDPASGKRFAKGHGHFKDMTSYEEL
HHHHHHCCCCCCCCEECCCCEECCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
KAAWDATLREITRMSVIVENAIDLGLEREVPDILCSALTDDCIGRGKTLKEGGAVYDYIS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
GLQVGIANLSDSLAALKKLVFEEGRLTPEELWKALESDFAGERGEDIRQMLINDAPKYGN
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCC
DDDYADSLVVEAYDTYIDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGKGTLATPDG
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCC
RHAGTPLAEGCSPEHSMDKKGPTSVLKSVAKLPTDEIVGGVLLNQKVNPQTLAKEEDKLK
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHCHHHHHH
LMALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSKATQD
HHHHHHHHHHHHCCCEEEEEEECHHHHHCCHHCCHHHHCEEEEEHHHHHHHHHHHHHHHH
DIIERTEHTL
HHHHHHHCCC
>Mature Secondary Structure 
THYREAVLDKKPYIDAERAILATEAYQKHQNKPANLKRAYMLQTILENMTIYIEDESLI
CCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECCCEE
AGNQASSNKDAPIFPEYTLEFVLNELDLFEKRDGDVFYITEETKQQLRDIAPFWENNNLR
ECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCCCCCE
ARCGVLLPEEVQVYMETGFFGMEGKMNSGDAHLAVNYQKLLEHGLKGFEERARAAKAALD
EEECCCCHHHHHHHHHCCEECCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHEE
LTIPENIDKYHFYDSVFIVIDAVKTYAKRYAKLARELAKTAKPERQAELLDIARICDKVP
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
YEPAKTFAEAVQSVWFIQCILQIESNGHSLSYGRFDQYMYPYVKADLEAGRETEDTIVER
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
LTNLWIKTLTINKVRSQAHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLRSVAQTK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHC
LPQPNLTVRYHKGLDNTFMNECIEVMKLGFGMPAMNNDEIIIPSFIKKGVSEEDAYDYSA
CCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHCCCCCCCCCCHHH
IGCVETAVPGKWGYRCTGMSYINFPKILLITMNDGIDPASGKRFAKGHGHFKDMTSYEEL
HHHHHHCCCCCCCCEECCCCEECCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
KAAWDATLREITRMSVIVENAIDLGLEREVPDILCSALTDDCIGRGKTLKEGGAVYDYIS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
GLQVGIANLSDSLAALKKLVFEEGRLTPEELWKALESDFAGERGEDIRQMLINDAPKYGN
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCC
DDDYADSLVVEAYDTYIDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGKGTLATPDG
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCC
RHAGTPLAEGCSPEHSMDKKGPTSVLKSVAKLPTDEIVGGVLLNQKVNPQTLAKEEDKLK
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHCHHHHHH
LMALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSKATQD
HHHHHHHHHHHHCCCEEEEEEECHHHHHCCHHCCHHHHCEEEEEHHHHHHHHHHHHHHHH
DIIERTEHTL
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]