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The map label for this gene is pulA

Identifier: 71911493

GI number: 71911493

Start: 1638902

End: 1639783

Strand: Reverse

Name: pulA

Synonym: M5005_Spy_1680

Alternate gene names: NA

Gene position: 1639783-1638902 (Counterclockwise)

Preceding gene: 71911494

Following gene: 71911492

Centisome position: 89.19

GC content: 41.72

Gene sequence:

>882_bases
TTGAATGTTATACTAAATGGAGGTCTTATGAAAAAGAAAGTCAACCAAGGATCAAAGCGCTATCAATATCTGTTAAAAAA
GTGGGGGATAGGTTTTGTAATCGCTGCAACTGGGACTGTCGTGTTAGGGTGCACCCCTAGTATCTTAACACATCAAGTTG
CTGCTAAAACCATTGTTGGACTAGCCCGCGATGAAGCTCAACAAGGAGATGGCAATGCTAAATCTGGTGATGGTCTTCAA
TCGTCTAGCAAGGAGGCAAAACCAGTTTTAGACAGCTCGTCAGCTAATCCTGCTAGTATTGCTGAGCATCATTTGCGTAT
GCATTTTAAAACATTGCCAGCTGGTGAGTCGCTAGGAAGCTTGGGACTTTGGGTGTGGGGAGATGTGGATCAACCTTCAA
AGGATTGGCCAAATGGTGCTATCACCATGACAAAAGCGAAAAAAGATGACTATGGCTATTATCTAGATGTGCCACTAGCA
GCTAAACACCGCCAGCAAGTGTCTTATCTCATTAATAATAAAGCTGGAGAGAATCTTTCAAAGGACCAGCACATCTCGCT
TCTCACGCCAAAAATGAATGAAGTTTGGATAGACGAGAATTACCATGCGCACGCTTATCGACCTTTGAAAAAAGGTTACC
TTCGAATCAACTACCACAATCAATCGGGACACTACGATAACTTAGCTGTCTGGACCTTTAAAGATGTCAAAACCCCAACG
ACCGACTGGCCAAATGGACTTGACTTGTCACATAAAGGGCATTATGGAGCTTATGTTGATGTCCCCTTAAAAGAAGGAGC
TAACGAAATCGGATTTTTAATCCTTGATAAAAGTAAGACAGGAGATGCTATTAAAGTGCAACCAAAAAGATTATCTATTT
AA

Upstream 100 bases:

>100_bases
AGATCCTATTAAGGGGTGATAGTCATTGTTTGAGCTGTGTTCTAAGTTGAAGTGCGGCATGTCTTGCAAGCGCTTGCGCA
ATGTGATAAGATAGTCTTGT

Downstream 100 bases:

>100_bases
AGAGTTAGACAATCATACTCAGGTTTTTGTCAAAGACACTGACCCAAAAGTTTACAACAATCCTTATTATATTGATCAGG
TTAGTCTCAAAGGAGCTGAA

Product: pullulanase

Products: NA

Alternate protein names: LPXTG-Motif Cell Wall Anchor Domain Protein; Alkaline Amylopullulanase; Cell Surface-Anchored Pullulanase; Type II Secretory Pathway Pullulanase PulA Glycosidase; Peptidoglycan Linked Protein Pullulanase; Surface-Anchored Amylopullulanase; Pullulanase Extracellular

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MNVILNGGLMKKKVNQGSKRYQYLLKKWGIGFVIAATGTVVLGCTPSILTHQVAAKTIVGLARDEAQQGDGNAKSGDGLQ
SSSKEAKPVLDSSSANPASIAEHHLRMHFKTLPAGESLGSLGLWVWGDVDQPSKDWPNGAITMTKAKKDDYGYYLDVPLA
AKHRQQVSYLINNKAGENLSKDQHISLLTPKMNEVWIDENYHAHAYRPLKKGYLRINYHNQSGHYDNLAVWTFKDVKTPT
TDWPNGLDLSHKGHYGAYVDVPLKEGANEIGFLILDKSKTGDAIKVQPKRLSI

Sequences:

>Translated_293_residues
MNVILNGGLMKKKVNQGSKRYQYLLKKWGIGFVIAATGTVVLGCTPSILTHQVAAKTIVGLARDEAQQGDGNAKSGDGLQ
SSSKEAKPVLDSSSANPASIAEHHLRMHFKTLPAGESLGSLGLWVWGDVDQPSKDWPNGAITMTKAKKDDYGYYLDVPLA
AKHRQQVSYLINNKAGENLSKDQHISLLTPKMNEVWIDENYHAHAYRPLKKGYLRINYHNQSGHYDNLAVWTFKDVKTPT
TDWPNGLDLSHKGHYGAYVDVPLKEGANEIGFLILDKSKTGDAIKVQPKRLSI
>Mature_293_residues
MNVILNGGLMKKKVNQGSKRYQYLLKKWGIGFVIAATGTVVLGCTPSILTHQVAAKTIVGLARDEAQQGDGNAKSGDGLQ
SSSKEAKPVLDSSSANPASIAEHHLRMHFKTLPAGESLGSLGLWVWGDVDQPSKDWPNGAITMTKAKKDDYGYYLDVPLA
AKHRQQVSYLINNKAGENLSKDQHISLLTPKMNEVWIDENYHAHAYRPLKKGYLRINYHNQSGHYDNLAVWTFKDVKTPT
TDWPNGLDLSHKGHYGAYVDVPLKEGANEIGFLILDKSKTGDAIKVQPKRLSI

Specific function: Unknown

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 32317; Mature: 32317

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVILNGGLMKKKVNQGSKRYQYLLKKWGIGFVIAATGTVVLGCTPSILTHQVAAKTIVG
CEEEECCCCHHHHHHCCHHHHHHHHHHCCCCEEEEECCEEEEECCHHHHHHHHHHHHHHH
LARDEAQQGDGNAKSGDGLQSSSKEAKPVLDSSSANPASIAEHHLRMHFKTLPAGESLGS
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEECCCCCCCCC
LGLWVWGDVDQPSKDWPNGAITMTKAKKDDYGYYLDVPLAAKHRQQVSYLINNKAGENLS
CEEEEEECCCCCCCCCCCCEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCC
KDQHISLLTPKMNEVWIDENYHAHAYRPLKKGYLRINYHNQSGHYDNLAVWTFKDVKTPT
CCCEEEEECCCCCEEEECCCCCCCEECCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCC
TDWPNGLDLSHKGHYGAYVDVPLKEGANEIGFLILDKSKTGDAIKVQPKRLSI
CCCCCCCCCCCCCCCCEEEEEEHHCCCCEEEEEEEECCCCCCEEEECCCCCCC
>Mature Secondary Structure
MNVILNGGLMKKKVNQGSKRYQYLLKKWGIGFVIAATGTVVLGCTPSILTHQVAAKTIVG
CEEEECCCCHHHHHHCCHHHHHHHHHHCCCCEEEEECCEEEEECCHHHHHHHHHHHHHHH
LARDEAQQGDGNAKSGDGLQSSSKEAKPVLDSSSANPASIAEHHLRMHFKTLPAGESLGS
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEECCCCCCCCC
LGLWVWGDVDQPSKDWPNGAITMTKAKKDDYGYYLDVPLAAKHRQQVSYLINNKAGENLS
CEEEEEECCCCCCCCCCCCEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCC
KDQHISLLTPKMNEVWIDENYHAHAYRPLKKGYLRINYHNQSGHYDNLAVWTFKDVKTPT
CCCEEEEECCCCCEEEECCCCCCCEECCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCC
TDWPNGLDLSHKGHYGAYVDVPLKEGANEIGFLILDKSKTGDAIKVQPKRLSI
CCCCCCCCCCCCCCCCEEEEEEHHCCCCEEEEEEEECCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA