| Definition | Streptococcus pyogenes MGAS5005 chromosome, complete genome. |
|---|---|
| Accession | NC_007297 |
| Length | 1,838,554 |
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The map label for this gene is pulA [H]
Identifier: 71911492
GI number: 71911492
Start: 1636258
End: 1638939
Strand: Reverse
Name: pulA [H]
Synonym: M5005_Spy_1679
Alternate gene names: 71911492
Gene position: 1638939-1636258 (Counterclockwise)
Preceding gene: 71911493
Following gene: 71911491
Centisome position: 89.14
GC content: 42.99
Gene sequence:
>2682_bases ATGCTATTAAAGTGCAACCAAAAAGATTATCTATTTAAAGAGTTAGACAATCATACTCAGGTTTTTGTCAAAGACACTGA CCCAAAAGTTTACAACAATCCTTATTATATTGATCAGGTTAGTCTCAAAGGAGCTGAACAAACCACGCCAAATGAGATTA AAGCCATTTTTACGACCTTAGATGGGCTTGATGAAGATGCGGTGAAACAAAACATCAAGATCACTGACAAAGCAGGGAAA ACTGTTGCAATTGATGAGTTGACACTTGACAGGGATAAGTCTGTAATGACATTAAAGGGTGATTTTAAGGCGCAAGGTGC AGTCTACACGGTTACATTTGGAGAAGTTAGCCAAGTCGCTCGCCAATCCTGGCAATTAAAAGATAAACTCTATGCTTACG ATGGTGAACTTGGAGCTACCCTAGCTAAGGATGGTTCTGTTGATTTAGCGCTATGGTCTCCAAGTGCTGATACTGTTAAG GTTGTCGTTTATGATAAACAAGATCAGACAAGGGTGGTTGGTCAAGCTGATTTGACCAAGTCGGACAAGGGTGTTTGGAG AGCTCATCTAACTTCTGACAGTGTCAAGGGCATTAGTGATTACACAGGCTACTATTACCTTTATGAAATCACGCGCGGTC AGGAAAAAGTCATGGTTTTGGATCCTTACGCCAAATCTCTCGCTGCCTGGAATGATGCGACTGCTACTGATGACATCAAA ACAGCAAAAGCTGCCTTTATTGATCCAAGCAAACTAGGACCAACAGGCCTTGATTTTGCCAAAATTAACAACTTTAAAAA GCGTGAAGACGCTATTATCTATGAAGCACATGTGCGAGATTTTACGTCAGATAAGGCTCTAGAAGGCAAGTTAACACACC CTTTTGGGACTTTTTCAGCTTTCGTTGAACAGCTAGACTATCTCAAAGACTTGGGGGTTACCCACGTTCAATTGCTACCG GTTTTGAGTTATTTTTATGCCAATGAGCTGGACAAGAGCCGCTCAACAGCCTACACGTCTTCAGACAATAATTACAACTG GGGTTATGACCCACAACACTACTTTGCCCTTTCTGGCATGTATTCGGCAAATCCTAATGACCCTGCTTTACGTATCGCAG AGCTTAAAAACCTTGTCAATGAGATTCACAAACGTGGTATGGGTGTTATTTTTGATGTGGTTTATAACCACACGGCTAGA ACCTATCTCTTTGAAGATTTGGAACCCAACTACTATCATTTTATGAATGCTGATGGTACAGCTAGAGAGAGTTTTGGCGG AGGTCGTCTAGGAACGACACATGCCATGAGTCGTCGTATCTTGGTGGATTCGATTACTTATCTGACTCGTGAATTCAAGG TAGATGGTTTTCGTTTCGACATGATGGGTGACCATGATGCGGCAGCTATTGAGCAAGCCTTTAAGGCAGCCAAAGCCATT AATCCAAATACCATTATGATTGGCGAAGGCTGGCGTACCTACCAAGGTGATGAGGGGAAAAAAGAAATTGCGGCAGATCA AGATTGGATGAAAGCAACCAATACGGTCGGTGTTTTCTCTGATGATATCAGAAATACCCTCAAGTCAGGTTTTCCAAATG AAGGCACAGCAGCCTTTATTACTGGTGGCGCAAAAAATCTAGAAGGTTTATTCAAAACGATCAAAGCACAGCCTGGTAAC TTTGAAGCAGATGCCCCAGGAGATGTAGTGCAGTATATTGCAGCCCATGACAACCTGACCTTACATGATGTCATTGCCAA ATCCATCAATAAGGATCCTAAAGTGGCTGAAGAAGAGATTCACAAGCGTATTCGTCTAGGAAATACCATGATTTTAACTG CTCAAGGGACTGCCTTTATCCATTCTGGTCAGGAATATGGACGAACCAAGCAGCTTCTAAATCCCGACTACAAGACAAAG GCGTCTGATGACAAGGTGCCAAATAAGGCGACTCTGATTGATGCTGTAGCGCAATACCCTTACTTCATCCACGATTCTTA TGATTCGTCTGATGCGGTCAATCATTTTGACTGGGCAAAGGCAACAGATTCCATAGCTCACCCGATTAGCAACCAAACAA AAGCCTATACACAGGGACTAATTGCGTTGCGTCGCTCAACAGATGCTTTTACAAAAGCAACCAAAGCTGAGGTAGATCGG GATGTGACCTTGATCACCCAAGCAGGACAAGATGGTATTCAACAAGAGGACCTCATCATGGGTTACCAAACAGTGGCATC AAATGGAGATCGCTATGCTGTCTTTGTCAATGCAGACAACAAGACCCGCAAGGTAGTTTTACCTCAAGCCTACCGCTATT TGCTAGGAGCCCAAGTGCTTGTTGATGCTGAGCAAGCTGGTGTTACTGCCATTGCTAAGCCTAAGGGAGTCCAGTTTACC AAAGAAGGCTTGACTATTGAAGGCCTAACTGCCCTGGTCCTCAAAGTATCCTCAAAAACGGCTAATCCCTCTCAGCAAAA GAGTCAGACAGACAATCATCAAACCAAAACACCAGATGGCTCAAAAGACCTAGACAAATCATTAATGACTAGACCAAAAA GAGCTAAAACAAACCAAAAGCTCCCAAAAACGGGTGAAGCCTCCTCAAAAGGCTTATTAGCAGCTGGAATAGCTCTGCTT TTATTGGCTATTAGCCTGTTGATGAAGCGCCAAAAAGATTAG
Upstream 100 bases:
>100_bases TTGTCACATAAAGGGCATTATGGAGCTTATGTTGATGTCCCCTTAAAAGAAGGAGCTAACGAAATCGGATTTTTAATCCT TGATAAAAGTAAGACAGGAG
Downstream 100 bases:
>100_bases GGTGGTGTTAATAGAGACAAAAAGATTGCTGTTTGCGGATTCTTTTTGACGGCTATAAAAGCTCTGAGCTGTGAAAAGAC CTTGTGATTGAGAAAATTGC
Product: pullulanase
Products: NA
Alternate protein names: Alpha-dextrin endo-1,6-alpha-glucosidase; Pullulan 6-glucanohydrolase [H]
Number of amino acids: Translated: 893; Mature: 893
Protein sequence:
>893_residues MLLKCNQKDYLFKELDNHTQVFVKDTDPKVYNNPYYIDQVSLKGAEQTTPNEIKAIFTTLDGLDEDAVKQNIKITDKAGK TVAIDELTLDRDKSVMTLKGDFKAQGAVYTVTFGEVSQVARQSWQLKDKLYAYDGELGATLAKDGSVDLALWSPSADTVK VVVYDKQDQTRVVGQADLTKSDKGVWRAHLTSDSVKGISDYTGYYYLYEITRGQEKVMVLDPYAKSLAAWNDATATDDIK TAKAAFIDPSKLGPTGLDFAKINNFKKREDAIIYEAHVRDFTSDKALEGKLTHPFGTFSAFVEQLDYLKDLGVTHVQLLP VLSYFYANELDKSRSTAYTSSDNNYNWGYDPQHYFALSGMYSANPNDPALRIAELKNLVNEIHKRGMGVIFDVVYNHTAR TYLFEDLEPNYYHFMNADGTARESFGGGRLGTTHAMSRRILVDSITYLTREFKVDGFRFDMMGDHDAAAIEQAFKAAKAI NPNTIMIGEGWRTYQGDEGKKEIAADQDWMKATNTVGVFSDDIRNTLKSGFPNEGTAAFITGGAKNLEGLFKTIKAQPGN FEADAPGDVVQYIAAHDNLTLHDVIAKSINKDPKVAEEEIHKRIRLGNTMILTAQGTAFIHSGQEYGRTKQLLNPDYKTK ASDDKVPNKATLIDAVAQYPYFIHDSYDSSDAVNHFDWAKATDSIAHPISNQTKAYTQGLIALRRSTDAFTKATKAEVDR DVTLITQAGQDGIQQEDLIMGYQTVASNGDRYAVFVNADNKTRKVVLPQAYRYLLGAQVLVDAEQAGVTAIAKPKGVQFT KEGLTIEGLTALVLKVSSKTANPSQQKSQTDNHQTKTPDGSKDLDKSLMTRPKRAKTNQKLPKTGEASSKGLLAAGIALL LLAISLLMKRQKD
Sequences:
>Translated_893_residues MLLKCNQKDYLFKELDNHTQVFVKDTDPKVYNNPYYIDQVSLKGAEQTTPNEIKAIFTTLDGLDEDAVKQNIKITDKAGK TVAIDELTLDRDKSVMTLKGDFKAQGAVYTVTFGEVSQVARQSWQLKDKLYAYDGELGATLAKDGSVDLALWSPSADTVK VVVYDKQDQTRVVGQADLTKSDKGVWRAHLTSDSVKGISDYTGYYYLYEITRGQEKVMVLDPYAKSLAAWNDATATDDIK TAKAAFIDPSKLGPTGLDFAKINNFKKREDAIIYEAHVRDFTSDKALEGKLTHPFGTFSAFVEQLDYLKDLGVTHVQLLP VLSYFYANELDKSRSTAYTSSDNNYNWGYDPQHYFALSGMYSANPNDPALRIAELKNLVNEIHKRGMGVIFDVVYNHTAR TYLFEDLEPNYYHFMNADGTARESFGGGRLGTTHAMSRRILVDSITYLTREFKVDGFRFDMMGDHDAAAIEQAFKAAKAI NPNTIMIGEGWRTYQGDEGKKEIAADQDWMKATNTVGVFSDDIRNTLKSGFPNEGTAAFITGGAKNLEGLFKTIKAQPGN FEADAPGDVVQYIAAHDNLTLHDVIAKSINKDPKVAEEEIHKRIRLGNTMILTAQGTAFIHSGQEYGRTKQLLNPDYKTK ASDDKVPNKATLIDAVAQYPYFIHDSYDSSDAVNHFDWAKATDSIAHPISNQTKAYTQGLIALRRSTDAFTKATKAEVDR DVTLITQAGQDGIQQEDLIMGYQTVASNGDRYAVFVNADNKTRKVVLPQAYRYLLGAQVLVDAEQAGVTAIAKPKGVQFT KEGLTIEGLTALVLKVSSKTANPSQQKSQTDNHQTKTPDGSKDLDKSLMTRPKRAKTNQKLPKTGEASSKGLLAAGIALL LLAISLLMKRQKD >Mature_893_residues MLLKCNQKDYLFKELDNHTQVFVKDTDPKVYNNPYYIDQVSLKGAEQTTPNEIKAIFTTLDGLDEDAVKQNIKITDKAGK TVAIDELTLDRDKSVMTLKGDFKAQGAVYTVTFGEVSQVARQSWQLKDKLYAYDGELGATLAKDGSVDLALWSPSADTVK VVVYDKQDQTRVVGQADLTKSDKGVWRAHLTSDSVKGISDYTGYYYLYEITRGQEKVMVLDPYAKSLAAWNDATATDDIK TAKAAFIDPSKLGPTGLDFAKINNFKKREDAIIYEAHVRDFTSDKALEGKLTHPFGTFSAFVEQLDYLKDLGVTHVQLLP VLSYFYANELDKSRSTAYTSSDNNYNWGYDPQHYFALSGMYSANPNDPALRIAELKNLVNEIHKRGMGVIFDVVYNHTAR TYLFEDLEPNYYHFMNADGTARESFGGGRLGTTHAMSRRILVDSITYLTREFKVDGFRFDMMGDHDAAAIEQAFKAAKAI NPNTIMIGEGWRTYQGDEGKKEIAADQDWMKATNTVGVFSDDIRNTLKSGFPNEGTAAFITGGAKNLEGLFKTIKAQPGN FEADAPGDVVQYIAAHDNLTLHDVIAKSINKDPKVAEEEIHKRIRLGNTMILTAQGTAFIHSGQEYGRTKQLLNPDYKTK ASDDKVPNKATLIDAVAQYPYFIHDSYDSSDAVNHFDWAKATDSIAHPISNQTKAYTQGLIALRRSTDAFTKATKAEVDR DVTLITQAGQDGIQQEDLIMGYQTVASNGDRYAVFVNADNKTRKVVLPQAYRYLLGAQVLVDAEQAGVTAIAKPKGVQFT KEGLTIEGLTALVLKVSSKTANPSQQKSQTDNHQTKTPDGSKDLDKSLMTRPKRAKTNQKLPKTGEASSKGLLAAGIALL LLAISLLMKRQKD
Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]
COG id: COG1523
COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the glycosyl hydrolase 13 family [H]
Homologues:
Organism=Escherichia coli, GI2367229, Length=355, Percent_Identity=25.3521126760563, Blast_Score=89, Evalue=1e-18,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR006047 - InterPro: IPR004193 - InterPro: IPR017853 - InterPro: IPR013781 - InterPro: IPR013783 - InterPro: IPR014756 - InterPro: IPR005323 - InterPro: IPR011840 [H]
Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48; PF03714 PUD [H]
EC number: =3.2.1.41 [H]
Molecular weight: Translated: 98909; Mature: 98909
Theoretical pI: Translated: 6.66; Mature: 6.66
Prosite motif: PS50847 GRAM_POS_ANCHORING
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.1 %Cys (Translated Protein) 1.7 %Met (Translated Protein) 1.8 %Cys+Met (Translated Protein) 0.1 %Cys (Mature Protein) 1.7 %Met (Mature Protein) 1.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MLLKCNQKDYLFKELDNHTQVFVKDTDPKVYNNPYYIDQVSLKGAEQTTPNEIKAIFTTL CEEECCCCHHHHHHCCCCEEEEEECCCCCEECCCEEEEEEEECCCCCCCHHHHHHHHHHH DGLDEDAVKQNIKITDKAGKTVAIDELTLDRDKSVMTLKGDFKAQGAVYTVTFGEVSQVA CCCCHHHHHCCCEEEECCCCEEEEEEEEECCCCCEEEEECCCCCCCEEEEEEHHHHHHHH RQSWQLKDKLYAYDGELGATLAKDGSVDLALWSPSADTVKVVVYDKQDQTRVVGQADLTK HHHHHHHHHHEEECCCCCCEEECCCCEEEEEECCCCCEEEEEEECCCCCEEEECCCCCCC SDKGVWRAHLTSDSVKGISDYTGYYYLYEITRGQEKVMVLDPYAKSLAAWNDATATDDIK CCCCEEEEEECCCCCCCCHHCCCEEEEEEEECCCCEEEEECCHHHHHHHCCCCCCCCCHH TAKAAFIDPSKLGPTGLDFAKINNFKKREDAIIYEAHVRDFTSDKALEGKLTHPFGTFSA HHHHHCCCCHHCCCCCCCHHHHCCCCCCCCCEEEEEHHCCCCCCCCCCCCCCCCCHHHHH FVEQLDYLKDLGVTHVQLLPVLSYFYANELDKSRSTAYTSSDNNYNWGYDPQHYFALSGM HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCEEEEECE YSANPNDPALRIAELKNLVNEIHKRGMGVIFDVVYNHTARTYLFEDLEPNYYHFMNADGT ECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCHHEEEEECCCCCEEEEECCCCC ARESFGGGRLGTTHAMSRRILVDSITYLTREFKVDGFRFDMMGDHDAAAIEQAFKAAKAI CHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCEEEECCCCCHHHHHHHHHHHHHCC NPNTIMIGEGWRTYQGDEGKKEIAADQDWMKATNTVGVFSDDIRNTLKSGFPNEGTAAFI CCCEEEECCCCCCCCCCCCCHHHCCCHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCEEEE TGGAKNLEGLFKTIKAQPGNFEADAPGDVVQYIAAHDNLTLHDVIAKSINKDPKVAEEEI ECCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHH HKRIRLGNTMILTAQGTAFIHSGQEYGRTKQLLNPDYKTKASDDKVPNKATLIDAVAQYP HHHHHCCCEEEEEECCCEEEECCHHHCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCC YFIHDSYDSSDAVNHFDWAKATDSIAHPISNQTKAYTQGLIALRRSTDAFTKATKAEVDR EEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCC DVTLITQAGQDGIQQEDLIMGYQTVASNGDRYAVFVNADNKTRKVVLPQAYRYLLGAQVL CEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCEEEECHHHHHHHHCCHHE VDAEQAGVTAIAKPKGVQFTKEGLTIEGLTALVLKVSSKTANPSQQKSQTDNHQTKTPDG EEHHHCCEEEEECCCCCEEECCCCEECCHHEEEEEECCCCCCCCHHHHCCCCCCCCCCCC SKDLDKSLMTRPKRAKTNQKLPKTGEASSKGLLAAGIALLLLAISLLMKRQKD CHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC >Mature Secondary Structure MLLKCNQKDYLFKELDNHTQVFVKDTDPKVYNNPYYIDQVSLKGAEQTTPNEIKAIFTTL CEEECCCCHHHHHHCCCCEEEEEECCCCCEECCCEEEEEEEECCCCCCCHHHHHHHHHHH DGLDEDAVKQNIKITDKAGKTVAIDELTLDRDKSVMTLKGDFKAQGAVYTVTFGEVSQVA CCCCHHHHHCCCEEEECCCCEEEEEEEEECCCCCEEEEECCCCCCCEEEEEEHHHHHHHH RQSWQLKDKLYAYDGELGATLAKDGSVDLALWSPSADTVKVVVYDKQDQTRVVGQADLTK HHHHHHHHHHEEECCCCCCEEECCCCEEEEEECCCCCEEEEEEECCCCCEEEECCCCCCC SDKGVWRAHLTSDSVKGISDYTGYYYLYEITRGQEKVMVLDPYAKSLAAWNDATATDDIK CCCCEEEEEECCCCCCCCHHCCCEEEEEEEECCCCEEEEECCHHHHHHHCCCCCCCCCHH TAKAAFIDPSKLGPTGLDFAKINNFKKREDAIIYEAHVRDFTSDKALEGKLTHPFGTFSA HHHHHCCCCHHCCCCCCCHHHHCCCCCCCCCEEEEEHHCCCCCCCCCCCCCCCCCHHHHH FVEQLDYLKDLGVTHVQLLPVLSYFYANELDKSRSTAYTSSDNNYNWGYDPQHYFALSGM HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCEEEEECE YSANPNDPALRIAELKNLVNEIHKRGMGVIFDVVYNHTARTYLFEDLEPNYYHFMNADGT ECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCHHEEEEECCCCCEEEEECCCCC ARESFGGGRLGTTHAMSRRILVDSITYLTREFKVDGFRFDMMGDHDAAAIEQAFKAAKAI CHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCEEEECCCCCHHHHHHHHHHHHHCC NPNTIMIGEGWRTYQGDEGKKEIAADQDWMKATNTVGVFSDDIRNTLKSGFPNEGTAAFI CCCEEEECCCCCCCCCCCCCHHHCCCHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCEEEE TGGAKNLEGLFKTIKAQPGNFEADAPGDVVQYIAAHDNLTLHDVIAKSINKDPKVAEEEI ECCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHH HKRIRLGNTMILTAQGTAFIHSGQEYGRTKQLLNPDYKTKASDDKVPNKATLIDAVAQYP HHHHHCCCEEEEEECCCEEEECCHHHCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCC YFIHDSYDSSDAVNHFDWAKATDSIAHPISNQTKAYTQGLIALRRSTDAFTKATKAEVDR EEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCC DVTLITQAGQDGIQQEDLIMGYQTVASNGDRYAVFVNADNKTRKVVLPQAYRYLLGAQVL CEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCEEEECHHHHHHHHCCHHE VDAEQAGVTAIAKPKGVQFTKEGLTIEGLTALVLKVSSKTANPSQQKSQTDNHQTKTPDG EEHHHCCEEEEECCCCCEEECCCCEECCHHEEEEEECCCCCCCCHHHHCCCCCCCCCCCC SKDLDKSLMTRPKRAKTNQKLPKTGEASSKGLLAAGIALLLLAISLLMKRQKD CHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9453151; 10360571 [H]