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The map label for this gene is glpF.2 [H]

Identifier: 71911386

GI number: 71911386

Start: 1534285

End: 1535133

Strand: Reverse

Name: glpF.2 [H]

Synonym: M5005_Spy_1573

Alternate gene names: 71911386

Gene position: 1535133-1534285 (Counterclockwise)

Preceding gene: 71911387

Following gene: 71911384

Centisome position: 83.5

GC content: 39.93

Gene sequence:

>849_bases
ATGGAAATGACATGGACTGTGAAATACATCACAGAATTTATCGCAACGGCTTTTTTGATTATTTTGGGAAATGGAGCTGT
TGCTAACGTCGACTTAAAAGGAACAAAAGGACATAACTCAGGTTGGTTAGTGATTGCTTTTGGCTATGGCTTAGGAGTTA
TGATGCCAGCTTTGATGTTTGGTAATGTATCTGGAAACCATATTAATCCTGCTTTCACAGTTGGACTAGCAGTATCAGGC
TTGTTCCCATGGGCTCACGTCTTACAATACGTTGTGGCACAATTACTTGGAGCTATCTTTGGTCAGTTGGTAGTGGTAAT
GGTTTATAAACCTTACTTTATGAAAACTGAAAATCCAAATCATGTTTTAGGCTCATTCTCAACGATTTCATCTCTTGATA
ATGGTCAAAAAGACAGTCATAAAGCCTCTTATATCAATGGTTTCTTAAACGAATTTGTAGGTTCATTTGTTCTTTTCTTT
GGTGCTTTGGCTTTGACTAAAAACTACTTTGGGGTAGAGTTAGTTGGTAAATTGATTGAAGCAGGCTACGACCAAACAAC
TGCAGCAACACAAATTTCACCATACGTGACAGGCTCATTGGCGGTTGCTCACATTGGTATTGGTTTCTTAGTTATGGTCT
TGGTTACTTCATTAGGTGGACCAACTGGGCCAGCACTAAACCCAGCGCGTGATTTTGGGCCTCGTTTGTTACACCACTTC
TTACCAAAATCAGTTCTTGGTCAAGCCAAAGGTGATTCAAAATGGTGGTATGCATGGGTTCCTGTAGTTGCACCTATCTT
AGCGGCGATAGTAGCCGTAGCAGCGTTTAAATACCTTTATATCAGATAA

Upstream 100 bases:

>100_bases
AATGATCATTTTATTTATTATTTTATTACCTTTACATTCATCAATAAGCCTGTTAAAATGGTATTTGTTATATTTTATTA
TTTGGAGGAAACCAGAAAAC

Downstream 100 bases:

>100_bases
AAAAAGAACTAAAGCTTCTTTTAGACTCAAGACAAGCTCGCTTAAGGAACTTGTCTTTTTTATATGAGTTAAGCGAAATC
TAATACAAACTTGACTGTCT

Product: aquaporin

Products: glycerol [Cytoplasm] [C]

Alternate protein names: Aquaglyceroporin; Glyceroaquaporin [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MEMTWTVKYITEFIATAFLIILGNGAVANVDLKGTKGHNSGWLVIAFGYGLGVMMPALMFGNVSGNHINPAFTVGLAVSG
LFPWAHVLQYVVAQLLGAIFGQLVVVMVYKPYFMKTENPNHVLGSFSTISSLDNGQKDSHKASYINGFLNEFVGSFVLFF
GALALTKNYFGVELVGKLIEAGYDQTTAATQISPYVTGSLAVAHIGIGFLVMVLVTSLGGPTGPALNPARDFGPRLLHHF
LPKSVLGQAKGDSKWWYAWVPVVAPILAAIVAVAAFKYLYIR

Sequences:

>Translated_282_residues
MEMTWTVKYITEFIATAFLIILGNGAVANVDLKGTKGHNSGWLVIAFGYGLGVMMPALMFGNVSGNHINPAFTVGLAVSG
LFPWAHVLQYVVAQLLGAIFGQLVVVMVYKPYFMKTENPNHVLGSFSTISSLDNGQKDSHKASYINGFLNEFVGSFVLFF
GALALTKNYFGVELVGKLIEAGYDQTTAATQISPYVTGSLAVAHIGIGFLVMVLVTSLGGPTGPALNPARDFGPRLLHHF
LPKSVLGQAKGDSKWWYAWVPVVAPILAAIVAVAAFKYLYIR
>Mature_282_residues
MEMTWTVKYITEFIATAFLIILGNGAVANVDLKGTKGHNSGWLVIAFGYGLGVMMPALMFGNVSGNHINPAFTVGLAVSG
LFPWAHVLQYVVAQLLGAIFGQLVVVMVYKPYFMKTENPNHVLGSFSTISSLDNGQKDSHKASYINGFLNEFVGSFVLFF
GALALTKNYFGVELVGKLIEAGYDQTTAATQISPYVTGSLAVAHIGIGFLVMVLVTSLGGPTGPALNPARDFGPRLLHHF
LPKSVLGQAKGDSKWWYAWVPVVAPILAAIVAVAAFKYLYIR

Specific function: Mixed channel protein that transports both water and glycerol [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=281, Percent_Identity=31.3167259786477, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI22538420, Length=279, Percent_Identity=30.4659498207885, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI157266307, Length=273, Percent_Identity=28.5714285714286, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI4502187, Length=265, Percent_Identity=29.0566037735849, Blast_Score=78, Evalue=7e-15,
Organism=Escherichia coli, GI1790362, Length=288, Percent_Identity=30.5555555555556, Blast_Score=86, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17558372, Length=283, Percent_Identity=27.5618374558304, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71994009, Length=271, Percent_Identity=26.5682656826568, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71992966, Length=277, Percent_Identity=27.0758122743682, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17544068, Length=271, Percent_Identity=24.7232472324723, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17531431, Length=279, Percent_Identity=26.8817204301075, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17531429, Length=279, Percent_Identity=26.8817204301075, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321054, Length=229, Percent_Identity=31.8777292576419, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI45550503, Length=275, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 30324; Mature: 30324

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMTWTVKYITEFIATAFLIILGNGAVANVDLKGTKGHNSGWLVIAFGYGLGVMMPALMF
CCCEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHH
GNVSGNHINPAFTVGLAVSGLFPWAHVLQYVVAQLLGAIFGQLVVVMVYKPYFMKTENPN
CCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCC
HVLGSFSTISSLDNGQKDSHKASYINGFLNEFVGSFVLFFGALALTKNYFGVELVGKLIE
CHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AGYDQTTAATQISPYVTGSLAVAHIGIGFLVMVLVTSLGGPTGPALNPARDFGPRLLHHF
CCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
LPKSVLGQAKGDSKWWYAWVPVVAPILAAIVAVAAFKYLYIR
CCHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEMTWTVKYITEFIATAFLIILGNGAVANVDLKGTKGHNSGWLVIAFGYGLGVMMPALMF
CCCEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHH
GNVSGNHINPAFTVGLAVSGLFPWAHVLQYVVAQLLGAIFGQLVVVMVYKPYFMKTENPN
CCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCC
HVLGSFSTISSLDNGQKDSHKASYINGFLNEFVGSFVLFFGALALTKNYFGVELVGKLIE
CHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AGYDQTTAATQISPYVTGSLAVAHIGIGFLVMVLVTSLGGPTGPALNPARDFGPRLLHHF
CCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
LPKSVLGQAKGDSKWWYAWVPVVAPILAAIVAVAAFKYLYIR
CCHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]