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The map label for this gene is thiI [H]

Identifier: 71910444

GI number: 71910444

Start: 634655

End: 635869

Strand: Direct

Name: thiI [H]

Synonym: M5005_Spy_0631

Alternate gene names: 71910444

Gene position: 634655-635869 (Clockwise)

Preceding gene: 71910443

Following gene: 71910445

Centisome position: 34.52

GC content: 41.56

Gene sequence:

>1215_bases
ATGGACTATTCAGAAATTATGGTTCGCCATGGTGAACTATCAACTAAAGGGAAGAATCGCATGCGATTCATTAACAAACT
TAAAAACAATATTCAAGACGTACTGGCTCCTTTCCCAGCCATTACTGTCCGTTCAGATCGTGATAGGACCCATGTGTCTC
TAAATGGCACAGATTATCAGCCTATTGTAGAAGCTTTAAAGCTGGTGTTTGGTGTTCAAGCTCTATCTCCTGTTTATAAA
CTTGAAAAAAGTGTCCCTCTTTTGGTAACAGCTGTTCAAGATATCATGACTTCTCTTTATCGTGATGGACTGACGTTTAA
GATTGCCACTAAGCGTAGTGATCACGCGTTTGAATTGGATAGTCGTGAGCTTAACAGTCTTCTTGGTGGAGCTGTTTTTG
AGGTTTTACCAAATATCCAAGCCCAGATGAAACATCCCGATGTGACTCTCAAAGTCGAAATTCGTGATGAAGCAGCTTAC
ATTTCTTATGAAGAGATTAAGGGAACAGGCGGGTTACCGGTAGGAACGTCAGGGAAAGGGATGCTGATGTTATCGGGCGG
TATTGATTCACCAGTAGCTGGGTATTTAGCCCTTAAACGTGGCCTTGATATTGAAGTTGTTCATTTTGCTAGCCCTCCGT
ACACCAGTCCAGGTGCCTTGGCTAAAGCCCAAGACCTTACTCGTCGATTAACTCGTTTTGGTGGAAATATCCAATTTATC
GAGGTTCCTTTTACAGAAATTCAAGAAGAAATTAAAAATAAGGCACCAGAGGCTTACCTTATGACATTAACCCGCCGCTT
TATGATGCGCATCACAGATGCCATTCGTGAGCAACGTAAGGGTCTTGTTATTGTTAACGGTGAGAGCCTAGGTCAGGTTG
CTAGTCAAACCCTTGAAAGCATGCAGGCCATTAATGCGGTAACATCAACTCCAATTATTAGACCAGTCGTGACAATGGAT
AAACTTGAAATTATCGAGATGGCTCAGGCGATTGATACTTTTGACATTTCTATTCAACCGTTCGAAGATTGCTGTACTAT
TTTTGCACCAGACCGTCCTAAAACCAACCCTAAACTAGGTAATGCTGAAAAATATGAAGAACGCTTTGATATTGATGGTC
TTGTCCAGCGTGCTGTGTCAGGAATTGTTGTCACAGAAATCACACCTGAAATCGTCAATGATGAGGTTGAAAATCTCATT
GATGCTTTACTTTAA

Upstream 100 bases:

>100_bases
GTCTAGACGATGACAACGATATGGGACAAGTAGAGCAGTTTTTGACCATATTCAAACAGATTTATGATAAAACACAGAAA
GTTAGGTAAGATTAGGTAAG

Downstream 100 bases:

>100_bases
AAGAAAAGATCAAGTGTAAATCTTTTAGTAGTCAGTAATGATACTATTGAAAAGCCTCTATAAGGGCTTTTTTGCTGCAA
TGAAGGAGGAAAATTTTGAG

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MDYSEIMVRHGELSTKGKNRMRFINKLKNNIQDVLAPFPAITVRSDRDRTHVSLNGTDYQPIVEALKLVFGVQALSPVYK
LEKSVPLLVTAVQDIMTSLYRDGLTFKIATKRSDHAFELDSRELNSLLGGAVFEVLPNIQAQMKHPDVTLKVEIRDEAAY
ISYEEIKGTGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGLDIEVVHFASPPYTSPGALAKAQDLTRRLTRFGGNIQFI
EVPFTEIQEEIKNKAPEAYLMTLTRRFMMRITDAIREQRKGLVIVNGESLGQVASQTLESMQAINAVTSTPIIRPVVTMD
KLEIIEMAQAIDTFDISIQPFEDCCTIFAPDRPKTNPKLGNAEKYEERFDIDGLVQRAVSGIVVTEITPEIVNDEVENLI
DALL

Sequences:

>Translated_404_residues
MDYSEIMVRHGELSTKGKNRMRFINKLKNNIQDVLAPFPAITVRSDRDRTHVSLNGTDYQPIVEALKLVFGVQALSPVYK
LEKSVPLLVTAVQDIMTSLYRDGLTFKIATKRSDHAFELDSRELNSLLGGAVFEVLPNIQAQMKHPDVTLKVEIRDEAAY
ISYEEIKGTGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGLDIEVVHFASPPYTSPGALAKAQDLTRRLTRFGGNIQFI
EVPFTEIQEEIKNKAPEAYLMTLTRRFMMRITDAIREQRKGLVIVNGESLGQVASQTLESMQAINAVTSTPIIRPVVTMD
KLEIIEMAQAIDTFDISIQPFEDCCTIFAPDRPKTNPKLGNAEKYEERFDIDGLVQRAVSGIVVTEITPEIVNDEVENLI
DALL
>Mature_404_residues
MDYSEIMVRHGELSTKGKNRMRFINKLKNNIQDVLAPFPAITVRSDRDRTHVSLNGTDYQPIVEALKLVFGVQALSPVYK
LEKSVPLLVTAVQDIMTSLYRDGLTFKIATKRSDHAFELDSRELNSLLGGAVFEVLPNIQAQMKHPDVTLKVEIRDEAAY
ISYEEIKGTGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGLDIEVVHFASPPYTSPGALAKAQDLTRRLTRFGGNIQFI
EVPFTEIQEEIKNKAPEAYLMTLTRRFMMRITDAIREQRKGLVIVNGESLGQVASQTLESMQAINAVTSTPIIRPVVTMD
KLEIIEMAQAIDTFDISIQPFEDCCTIFAPDRPKTNPKLGNAEKYEERFDIDGLVQRAVSGIVVTEITPEIVNDEVENLI
DALL

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=384, Percent_Identity=28.90625, Blast_Score=135, Evalue=6e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 44814; Mature: 44814

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYSEIMVRHGELSTKGKNRMRFINKLKNNIQDVLAPFPAITVRSDRDRTHVSLNGTDYQ
CCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCHH
PIVEALKLVFGVQALSPVYKLEKSVPLLVTAVQDIMTSLYRDGLTFKIATKRSDHAFELD
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEECC
SRELNSLLGGAVFEVLPNIQAQMKHPDVTLKVEIRDEAAYISYEEIKGTGGLPVGTSGKG
HHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEEEECCCCEEEHHHHCCCCCCCCCCCCCE
MLMLSGGIDSPVAGYLALKRGLDIEVVHFASPPYTSPGALAKAQDLTRRLTRFGGNIQFI
EEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEE
EVPFTEIQEEIKNKAPEAYLMTLTRRFMMRITDAIREQRKGLVIVNGESLGQVASQTLES
ECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHH
MQAINAVTSTPIIRPVVTMDKLEIIEMAQAIDTFDISIQPFEDCCTIFAPDRPKTNPKLG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEECCHHHHHEEECCCCCCCCCCCC
NAEKYEERFDIDGLVQRAVSGIVVTEITPEIVNDEVENLIDALL
CHHHHHHHCCHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MDYSEIMVRHGELSTKGKNRMRFINKLKNNIQDVLAPFPAITVRSDRDRTHVSLNGTDYQ
CCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCHH
PIVEALKLVFGVQALSPVYKLEKSVPLLVTAVQDIMTSLYRDGLTFKIATKRSDHAFELD
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEECC
SRELNSLLGGAVFEVLPNIQAQMKHPDVTLKVEIRDEAAYISYEEIKGTGGLPVGTSGKG
HHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEEEECCCCEEEHHHHCCCCCCCCCCCCCE
MLMLSGGIDSPVAGYLALKRGLDIEVVHFASPPYTSPGALAKAQDLTRRLTRFGGNIQFI
EEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEE
EVPFTEIQEEIKNKAPEAYLMTLTRRFMMRITDAIREQRKGLVIVNGESLGQVASQTLES
ECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHH
MQAINAVTSTPIIRPVVTMDKLEIIEMAQAIDTFDISIQPFEDCCTIFAPDRPKTNPKLG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEECCHHHHHEEECCCCCCCCCCCC
NAEKYEERFDIDGLVQRAVSGIVVTEITPEIVNDEVENLIDALL
CHHHHHHHCCHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]