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The map label for this gene is endA [H]

Identifier: 71910399

GI number: 71910399

Start: 584152

End: 584691

Strand: Direct

Name: endA [H]

Synonym: M5005_Spy_0586

Alternate gene names: 71910399

Gene position: 584152-584691 (Clockwise)

Preceding gene: 71910398

Following gene: 71910400

Centisome position: 31.77

GC content: 42.22

Gene sequence:

>540_bases
ATGTTAACAAGGAAAAAACGAAAAAGGGTGCAAGAGCAAAAGGTTCCTAAAAGAGCTAATGCCTTACTTACTAAGTCGAC
ACGACAGTATCGAAATCGCTATCAAACGGATAATGCTTATCGTAACTTTAAGCCAGCAGGTTGGCACCGATTGCACCACT
TAAAAGGAAGTTATGATCACGCGGTTGATAGAGGGCATTTGCTAGGTTATGCTTTAGTTGGTGGACTGAAAGGTTTTGAT
GCTTCTACTGGTAATCCTGATAATATTGCCACTCAACTAAGTTGGGCTAATCAAGCAAATAAACCCTACTTGACAGGTCA
GAATTACTATGAAGGTTTAGTACGTCGTGCTTTAGATAAGGGGCATCGCGTCCGCTACCGTGTCACCCTACTTTATGATG
GTGATAATCTACTAGCCAGCGGTAGCCATTTGGAGGCTAAATCCTCTGATGACAGCTTGACTTTTAACGTGTTTGTTCCA
AATGTCCAAGCAGGATTGACCGTTGACTATCGGACGGGACAAATAGCTATCAATCTCTAA

Upstream 100 bases:

>100_bases
GTCCATGGCATATTTTATCTATGGATAAGTGGGCAGAGTTAGTGAATAAATTTACTGGAAAGTAGGCCTTGCCTACTTTT
TATAGCATCTGAGAGGAAGT

Downstream 100 bases:

>100_bases
GCGAAAAGTCACTGATTTAAGATTGTCACTAAGCACTTTTTTTGATAGAATAGAACTAATTGAATAAGTGTCTATGAGAC
TAGTACTAATAGAGAAATTA

Product: DNA-entry nuclease

Products: NA

Alternate protein names: Competence-specific nuclease [H]

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MLTRKKRKRVQEQKVPKRANALLTKSTRQYRNRYQTDNAYRNFKPAGWHRLHHLKGSYDHAVDRGHLLGYALVGGLKGFD
ASTGNPDNIATQLSWANQANKPYLTGQNYYEGLVRRALDKGHRVRYRVTLLYDGDNLLASGSHLEAKSSDDSLTFNVFVP
NVQAGLTVDYRTGQIAINL

Sequences:

>Translated_179_residues
MLTRKKRKRVQEQKVPKRANALLTKSTRQYRNRYQTDNAYRNFKPAGWHRLHHLKGSYDHAVDRGHLLGYALVGGLKGFD
ASTGNPDNIATQLSWANQANKPYLTGQNYYEGLVRRALDKGHRVRYRVTLLYDGDNLLASGSHLEAKSSDDSLTFNVFVP
NVQAGLTVDYRTGQIAINL
>Mature_179_residues
MLTRKKRKRVQEQKVPKRANALLTKSTRQYRNRYQTDNAYRNFKPAGWHRLHHLKGSYDHAVDRGHLLGYALVGGLKGFD
ASTGNPDNIATQLSWANQANKPYLTGQNYYEGLVRRALDKGHRVRYRVTLLYDGDNLLASGSHLEAKSSDDSLTFNVFVP
NVQAGLTVDYRTGQIAINL

Specific function: By degrading DNA that enters the cell, plays a role in the competence of cells to be transformed [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA/RNA non-specific endonuclease family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018524
- InterPro:   IPR001604 [H]

Pfam domain/function: PF01223 Endonuclease_NS [H]

EC number: 3.1.30.-

Molecular weight: Translated: 20302; Mature: 20302

Theoretical pI: Translated: 10.58; Mature: 10.58

Prosite motif: PS01070 NUCLEASE_NON_SPEC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRKKRKRVQEQKVPKRANALLTKSTRQYRNRYQTDNAYRNFKPAGWHRLHHLKGSYDH
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCHH
AVDRGHLLGYALVGGLKGFDASTGNPDNIATQLSWANQANKPYLTGQNYYEGLVRRALDK
HHCCCHHHHHHHHCCCCCCCCCCCCCCHHHEEEECCCCCCCCEECCCHHHHHHHHHHHCC
GHRVRYRVTLLYDGDNLLASGSHLEAKSSDDSLTFNVFVPNVQAGLTVDYRTGQIAINL
CCEEEEEEEEEECCCCEEECCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCEEEEEC
>Mature Secondary Structure
MLTRKKRKRVQEQKVPKRANALLTKSTRQYRNRYQTDNAYRNFKPAGWHRLHHLKGSYDH
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCHH
AVDRGHLLGYALVGGLKGFDASTGNPDNIATQLSWANQANKPYLTGQNYYEGLVRRALDK
HHCCCHHHHHHHHCCCCCCCCCCCCCCHHHEEEECCCCCCCCEECCCHHHHHHHHHHHCC
GHRVRYRVTLLYDGDNLLASGSHLEAKSSDDSLTFNVFVPNVQAGLTVDYRTGQIAINL
CCEEEEEEEEEECCCCEEECCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2359120; 11463916 [H]